Objective:To study the feasibility of repair of partial maxillary defect by distraction osteogenesis(DO) in zygoma.Methods:Segmental maxilla- zygoma defect was made in 3 dogs. A submerged DO distractor was anchoraged on the zygoma and maxilla across the defect,the zygoma was distracted with a rate of 1.0 mm/d for ten days,the process of bone formation was examined by X-ray.Results:All dogs were in health condition within the experimental period.New bone formation was observed 2~4 weeks after operation. Complete repair of the defects was observed 8 weeks after the completion of distraction.Conclusion:The partial maxillary defect may be repaired by DO in zygoma.

Aim To study the effect of Semen Sojae Preparatum isoflavone(SSPI)on proliferation and JAK2/STAT3 signal transduction pathway in rat vascular smooth muscle cell(VSMC)induced by Angiotensin Ⅱ(AngⅡ).Methods A cell proliferating model of VSMC induced by AngⅡ was established.The proliferation activity of VSMC was analyzed by MTT method.The expressions of angiotensin Ⅱ receptor 1(AT1R) were detected by RT-PCR method.The expressions of JAK2,STAT3 and phosphorylation protein were detected by Western blot.Results 100 ?g?L-1, 200 ?g?L-1 SSPI significantly inhibited the proliferation of VSMC induced by AngⅡ,and down-regulated the mRNA expression of AT1R.200 ?g?L-1 SSPI could significantly down-regulate the protein expressions of p-JAK2,p-STAT3.Conclusions The proliferation of VSMC induced by AngⅡcan be inhibited by SSPI.The mechanisms might be related to down-regulating the expressions of AT1R,and arresting the phosphorylation of JAK2/STAT3 signal transduction pathway.

BACKGROUND: The rehabilitation of maxillary loss is based on maxillary prosthesis,and the most difficult problem is that retention and support of maxillary prosthesis can hardly be achieved. The employment of implants in the zygoma region provides good basis for the retention and support of maxillary prosthesis. However,during implantation in the zygoma the pe ripheral tissues may be injured, and even induce failed implantation.OBJECTIVE: To study the bone structure of the zygoma so as to provide reference for implantation in this region.DESIGN:Randomized sampling for repeated observation measurement based on maxilla specimens.SETTING: The research was completed in the prosthodontic department of stomatological college and department of human anatomy of a military medical university.PARTICIPANTS:The experiment was carried out in the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA from March to May 2002. A total of 73 adult maxilla specimens(38 from males and 35 from females) were provided by the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA.METHODS: Quantitative measurement of bone structure of the zygoma region was made with the cursor ruler in the 73 specimens. T-test was used to analyze the measurement data.MAIN OUTCOME MEASURES: The data of measurement: bone thickness of the zygoma, width of the lower part of the zygoma, length of the zygoma, and the distance between lower part of the zygoma and orbital floor.RESULTS: ① Bone thickness gradually decreased from the interior-inferior borderline to the exterior-superior part of the zygoma. In males, the average bone thickness 10 mm,12 mm,14 mm and 16 mm away from the interior-inferior borderline of the zygoma was 6.97 mm, 6.45 mm, 5.75 mm and 5. 01 mm, respectively, in the upper part, and 7.03 mm, 6.69 mm, 6.28 mm and 5.89 mm in the lower part. In females, the average bone thickness 4 mm,6 mm, 8 mm and 10 mm away from the interior-inferior borderline was 6.95 mm,6. 55 mm,6.28 mm and 5.31 mm in the upper part,and 6.60 mm,6. 39 mm,6.26 mm and 5.75 mm in the lower part. ② The lower part of the zygoma was 21.39 mm wide in males and 17.20 mm in females. ③ The average length of the zygoma was 21. 12 mm in males and 19.58 mm in females. ④ The average distance between lower part of the zygoma and orbital floor was 4. 17 mm in males and 3.71 mm in females.CONCLUSION:In maxillary loss,the zygoma is an optimal region for double implants at a distance of more than 15 mm. In males, the implants of 3.6 -4.0 mm in diameter and 10 - 12 mm or 14 - 16 mm in lengthcan be employed; in females, the implants of 3.4 - 3.8 mm in diameter and 4 - 6 mm or 8- 10 mm in length can be employed.The differences in bone quantity between males and females are statistically significant in the zygoma,males' better than females', so they should be treated differently during clinical practice.

The paper discussed a variety of experimental designs of compound compatibility law of traditional Chinese medicine (TCM): study of whole formula and different ingredients of formula. The latter includes study of single ingredient, study of functional ingredient group, orthogonal design, clustering analysis, homogeneous design, factorial analysis and so on. It was proposed that experimental designs of formula should be based on the theory of TCM, and combined with modern sciences.

AIM: To explore the relationship between berberine content of Banxiaxiexin Decoction in rat serum and gastric motility. METHODS:Berberine content of Banxiaxiexin Decoction was determined by HPLC; Gastric residue was observed in all the rats with a marker of dextran blue 2000 to determine the capacity of gastric evacuation. RESULTS: Banxiaxiexin Decoction markly promoted gastric evacuation ( P

AIM: To investigate the contractile effect of berberine on antral smooth muscle cells in rat stomach. METHODS: Smooth muscle cells were isolated from the antrum of the SD rat stomach. Different doses of berberine were added into cell suspension. Contractibility of smooth muscle cells were measured by micrometry. RESULTS: Compared with the control group the length of the treated cells(0.5g?L -1 ,1g?L -1 ) was significantly short( P

Objective To study the mechanism of Hezhong Jiangni Formnla(HJF) in protecting esophageal mucosa.Methods Rat models of reflux esophagitis were established by cardioplasty,pyloric ligation and roux-Y type gastrojejunostomy to evaluate the degree of esophageal mucosa injury.After treatment of HJF,the gastric juice was collected with the method of pyloric ligation to determine its volume and acidity and the activity of pepsin.The gastrointestinal motility was observed after oral use of charcoal powder.The level of malondialydehyde(MDA)in esophageal mucosa was measured to evaluate the esophageal antioxidative ability.Results HJF relieved the injury of esophageal mucosa dose-dependently;it had no obvious influence on the volume and acidity of gastric juice and the activity of pepsin,but it could obviously improve the gastrointestinal motility.MDA level of esophageal mucosa in the model group was increased significantly as compared with the control group.Pretreatment with HJF could greatly inhibited the formation of MDA.Conclusion HJF has protective effect on esophageal mucosa,which may be related to the improvement of gastrointestinal motility and its antioxidant action.

Objective To investigate the expression of SOX2 in gastric carcinoma and to analyze its relationship with clinicopathological features.Methods Immunohistochemistry method was used to detect the level of SOX2 in 67 cases of gastric carcinoma group and 30 cases of normal gastric mucosa group.Results The positive expression rate of SOX2 in gastric carcinoma group (52.24 ％) was obviously lower than that in normal gastric mucosa group (93.33 ％) (x2 =16.326,P ＜ 0.01).The SOX2 expression was significantly correlated with differentiation,the depth of invasion,lymph node metastasis and TNM stage of the tumor (all P ＜ 0.05).Conclusions The low expression of SOX2 may contribute to the early carcinogenesis of gastric carcinoma,and the expression level of SOX2 is closely related with lymph node metastasis and TNM stage.The expression level of SOX2 is a useful marker for predicting the prognosis of gastric carcinoma.

Objective To evaluate and compare the efficacy of holmium laser enucleation of the prostate(HoLEP) and plasmakineticenucleation of the prostate(PKEP) for treating benign prostatic hyperplasia (BPH).Methods A total of 86 cases of BPH were selected from the Shandong Energy Zibo Mining Group Co.Ltd Central Hospital from January 2013 to June 2014.The patients received either HoLEP (40 cases) or PKEP(46 cases) treatment.Clinical data including postvoid residual volume (PVR),international prostate symptom score(IPSS),quality-of-life score(QOL score),maximum urinary flow rate (MFR) were evaluated before and 3 months after operation.Blood loss in operation,operation time,weight of resected prostate tissue,bladder irrigation time,catheterization time and length of hospital stay were also collected from the perioperative period.Results Three months after surgery,PVR,IPSS,QOL and MFR were all significantly improved in both HoLEP and PKEP groups compared with the data before operation (P =0.000),while there was no significant difference between the two groups(P＞0.05).Patients from the HoLEP group showed markedly lower values of bone loss in operation than that of PKEP group((69.5±23.6) ml vs.(87.5±38.0) ml,P=0.011).There were no significant differences in terms of other indexes (P＞ 0.05).Conclusion HoLEP and PKEP have similar efficiency in treating BPH,while HoLEP is suggested superior in regard of bone loss.

Objective To investigate the effect of hyperbaric oxygen therapy on serum procalcitonin (PCT), white blood cells(WBC) and high-sensitivity C-reactive protein (hs-CRP) levels in children with necrotising fasciitis (NF) and its efficacy, and explore the diagnosis value of above indicators.Methods From March 2011 to June 2014,50 cases children with acute necrotic fasciitis treated in Children's Hospital of Hebei Province as study group,which were randomly divided into hyperbaric oxygen group (n =25) and routine group (n =25) .The routine group received the routine therapy of incision and drainage to clear the lesion, hyperbaric oxygen group received hyperbaric oxygen therapy on the basis of routine group,while 50 healthy children were selected as control group.The serum PCT, WBC, hs-CRP levels, efficacy, complications, death and hospitalization time were observed and compared.Results The serum PCT, WBC and hs-CRP levels pre-treatment in study group were higher than those in control group(P<0.05).The area under the ROC curve of PCT and hs-CRP was 1.000,respectively, and WBC was 0.804, there were significant difference between PCT and WBC (Z=5.250,P=0.000), between hs-CRP and WBC (Z=5.037,P=0.000).After treatment, the wounds of 23 case patients (92.00%) were cured in hyperbaric oxygen group, and 21 cases in routine group (84.00%) , there were no significant difference in cure rate between two groups.There were six cases(24.00%) of complications and one case (4.00%) of death in hyperbaric oxygen group,while nine cases (36.00%) of complications and two cases (8.00%) of death, there were no significant difference in complications rate and death rate between two groups.The hospitalization time in hyperbaric oxygen group was (39.17 ±6.73) d, which was significantly lower than (52.13 ±4.28) d in routine group(P<0.05).Conclusion PCT and hs-CRP have certain value in diagnosis of children with acute necrotizing fasciitis; incision and drainage combined with hyperbaric oxygen therapy has a better clinical effect in the treatment of children with acute necrotizing fasciitis.