Aim To study the effect of Semen Sojae Preparatum isoflavone(SSPI)on proliferation and JAK2/STAT3 signal transduction pathway in rat vascular smooth muscle cell(VSMC)induced by Angiotensin Ⅱ(AngⅡ).Methods A cell proliferating model of VSMC induced by AngⅡ was established.The proliferation activity of VSMC was analyzed by MTT method.The expressions of angiotensin Ⅱ receptor 1(AT1R) were detected by RT-PCR method.The expressions of JAK2,STAT3 and phosphorylation protein were detected by Western blot.Results 100 ?g?L-1, 200 ?g?L-1 SSPI significantly inhibited the proliferation of VSMC induced by AngⅡ,and down-regulated the mRNA expression of AT1R.200 ?g?L-1 SSPI could significantly down-regulate the protein expressions of p-JAK2,p-STAT3.Conclusions The proliferation of VSMC induced by AngⅡcan be inhibited by SSPI.The mechanisms might be related to down-regulating the expressions of AT1R,and arresting the phosphorylation of JAK2/STAT3 signal transduction pathway.

Objective:To study the feasibility of repair of partial maxillary defect by distraction osteogenesis(DO) in zygoma.Methods:Segmental maxilla- zygoma defect was made in 3 dogs. A submerged DO distractor was anchoraged on the zygoma and maxilla across the defect,the zygoma was distracted with a rate of 1.0 mm/d for ten days,the process of bone formation was examined by X-ray.Results:All dogs were in health condition within the experimental period.New bone formation was observed 2~4 weeks after operation. Complete repair of the defects was observed 8 weeks after the completion of distraction.Conclusion:The partial maxillary defect may be repaired by DO in zygoma.

BACKGROUND: The rehabilitation of maxillary loss is based on maxillary prosthesis,and the most difficult problem is that retention and support of maxillary prosthesis can hardly be achieved. The employment of implants in the zygoma region provides good basis for the retention and support of maxillary prosthesis. However,during implantation in the zygoma the pe ripheral tissues may be injured, and even induce failed implantation.OBJECTIVE: To study the bone structure of the zygoma so as to provide reference for implantation in this region.DESIGN:Randomized sampling for repeated observation measurement based on maxilla specimens.SETTING: The research was completed in the prosthodontic department of stomatological college and department of human anatomy of a military medical university.PARTICIPANTS:The experiment was carried out in the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA from March to May 2002. A total of 73 adult maxilla specimens(38 from males and 35 from females) were provided by the Department of Human Anatomy,Fourth Military Medical University of Chinese PLA.METHODS: Quantitative measurement of bone structure of the zygoma region was made with the cursor ruler in the 73 specimens. T-test was used to analyze the measurement data.MAIN OUTCOME MEASURES: The data of measurement: bone thickness of the zygoma, width of the lower part of the zygoma, length of the zygoma, and the distance between lower part of the zygoma and orbital floor.RESULTS: ① Bone thickness gradually decreased from the interior-inferior borderline to the exterior-superior part of the zygoma. In males, the average bone thickness 10 mm,12 mm,14 mm and 16 mm away from the interior-inferior borderline of the zygoma was 6.97 mm, 6.45 mm, 5.75 mm and 5. 01 mm, respectively, in the upper part, and 7.03 mm, 6.69 mm, 6.28 mm and 5.89 mm in the lower part. In females, the average bone thickness 4 mm,6 mm, 8 mm and 10 mm away from the interior-inferior borderline was 6.95 mm,6. 55 mm,6.28 mm and 5.31 mm in the upper part,and 6.60 mm,6. 39 mm,6.26 mm and 5.75 mm in the lower part. ② The lower part of the zygoma was 21.39 mm wide in males and 17.20 mm in females. ③ The average length of the zygoma was 21. 12 mm in males and 19.58 mm in females. ④ The average distance between lower part of the zygoma and orbital floor was 4. 17 mm in males and 3.71 mm in females.CONCLUSION:In maxillary loss,the zygoma is an optimal region for double implants at a distance of more than 15 mm. In males, the implants of 3.6 -4.0 mm in diameter and 10 - 12 mm or 14 - 16 mm in lengthcan be employed; in females, the implants of 3.4 - 3.8 mm in diameter and 4 - 6 mm or 8- 10 mm in length can be employed.The differences in bone quantity between males and females are statistically significant in the zygoma,males' better than females', so they should be treated differently during clinical practice.

The paper discussed a variety of experimental designs of compound compatibility law of traditional Chinese medicine (TCM): study of whole formula and different ingredients of formula. The latter includes study of single ingredient, study of functional ingredient group, orthogonal design, clustering analysis, homogeneous design, factorial analysis and so on. It was proposed that experimental designs of formula should be based on the theory of TCM, and combined with modern sciences.

Objective To study the mechanism of Hezhong Jiangni Formnla(HJF) in protecting esophageal mucosa.Methods Rat models of reflux esophagitis were established by cardioplasty,pyloric ligation and roux-Y type gastrojejunostomy to evaluate the degree of esophageal mucosa injury.After treatment of HJF,the gastric juice was collected with the method of pyloric ligation to determine its volume and acidity and the activity of pepsin.The gastrointestinal motility was observed after oral use of charcoal powder.The level of malondialydehyde(MDA)in esophageal mucosa was measured to evaluate the esophageal antioxidative ability.Results HJF relieved the injury of esophageal mucosa dose-dependently;it had no obvious influence on the volume and acidity of gastric juice and the activity of pepsin,but it could obviously improve the gastrointestinal motility.MDA level of esophageal mucosa in the model group was increased significantly as compared with the control group.Pretreatment with HJF could greatly inhibited the formation of MDA.Conclusion HJF has protective effect on esophageal mucosa,which may be related to the improvement of gastrointestinal motility and its antioxidant action.

AIM: To explore the relationship between berberine content of Banxiaxiexin Decoction in rat serum and gastric motility. METHODS:Berberine content of Banxiaxiexin Decoction was determined by HPLC; Gastric residue was observed in all the rats with a marker of dextran blue 2000 to determine the capacity of gastric evacuation. RESULTS: Banxiaxiexin Decoction markly promoted gastric evacuation ( P

AIM: To investigate the contractile effect of berberine on antral smooth muscle cells in rat stomach. METHODS: Smooth muscle cells were isolated from the antrum of the SD rat stomach. Different doses of berberine were added into cell suspension. Contractibility of smooth muscle cells were measured by micrometry. RESULTS: Compared with the control group the length of the treated cells(0.5g?L -1 ,1g?L -1 ) was significantly short( P

Objective To observe the effects of uric acid (UA) on mitochondrial oxidative damage and apoptosis in renal tubular epithelial cells (HK-2),and investigate the possible mechanism.Methods HK-2 cells were exposed to UA (480 μmol/L,720 μmol/L) for different time (0 h,24 h,48 h)in vitro.The mitochondrial ROS production was detected by MitoSOX staining.The mitochondrial membrane potential was measured by JC-1 staining.The expressions of prohibitin and AIF were examined by Western blotting and irnmunofluorescence cytochemistry.The cell apoptosis was measured by Annexin V-FITC/PI staining.Results The mitochondrial ROS production in HK-2 cells exposed to 480 μ mol/L UA was increased than that of control group at 24 h (P ＜ 0.05),and increased gradually with UA concentration and incubation time increasing,while the mitochondrial membrane potential was reduced at the same time.There were no significant changes in AIF expression and apoptosis rate of HK -2 cells exposed to 480 μmol/L UA for 24 h compared with that of control group (P ＞ 0.05),while both of them were up-regulated when HK-2 cells were exposed to 480 μmol/L UA for 48 h and 720 μmol/L JA for 24 h and 48 h (P ＜ 0.05).The prohibitin expression in HK-2 cells exposed to 480 μmol/L UA was reduced than that of control group at 24 h (P ＜ 0.05),and down-regulated gradually with UA concentration and incubation time increasing.Conclusion Uric acid can induce the mitochondrial ROS production increased,the mitochondrial membrane potential reduced,the prohibitin expression down-regulated and the mitochondrial apoptosis pathway activated in HK-2 cells.

Objective To investigate the expression of SOX2 in gastric carcinoma and to analyze its relationship with clinicopathological features.Methods Immunohistochemistry method was used to detect the level of SOX2 in 67 cases of gastric carcinoma group and 30 cases of normal gastric mucosa group.Results The positive expression rate of SOX2 in gastric carcinoma group (52.24 ％) was obviously lower than that in normal gastric mucosa group (93.33 ％) (x2 =16.326,P ＜ 0.01).The SOX2 expression was significantly correlated with differentiation,the depth of invasion,lymph node metastasis and TNM stage of the tumor (all P ＜ 0.05).Conclusions The low expression of SOX2 may contribute to the early carcinogenesis of gastric carcinoma,and the expression level of SOX2 is closely related with lymph node metastasis and TNM stage.The expression level of SOX2 is a useful marker for predicting the prognosis of gastric carcinoma.

Objective To investigate the effect of benazepril on intergrin-linked kinase (ILK) and α-smooth muscle actin (α-SMA) expression in glomerular mesangial cells induced by high-glucose.Methods The mesangial cells from SD rat (HBZY-1) were cultured conventionally and randomly divided into four groups:normal glucose (D-glucose 5.5 mmol/L,group NG),mannitol-treated group (mannitol 20 mmol/L,group MG),high glucose (D-glucose 30 mmol/L,group HG),Benazepril-treated high glucose group (D-glucose 30 mmol/L + Benazepril 10 μmol/L,group ACEI).Cells from NG,MG,HG,ACEI gronps were harvested after 3,6,12,24,48 and 72 hours of treatment respectively.The mRNA expressions of ILK and α-SMA were detected by RT-PCR.The protein levels of ILK and α-SMA were detected by Western blotting and immunofluorescence.Results The expressions of ILK mRNA and protein in HG group were significantly increased compared with those in NG group (all P ＜ 0.05).The increased expressions of ILK and α-SMA in HG group were time-dependent and the expression reached the peak at 48 h (ILK,P ＜ 0.05) or 72 h (α-SMA,P ＜ 0.01).The expressions of ILK and α-SMA in ACEI group were lower than those in HG group (all P ＜ 0.01),but failed to rescue to the same level as those in NG.There was no significant differences of ILK expressions between MG group and NG group at the same time point (P ＞ 0.05).The expressions of α-SMA mRNA and protein in MG were higher than that in NG (P ＜ 0.05),which suggest that high osmotic pressure could cause the increasing of α-SMA.Conclusions Benazepril can decrease the expressions of ILK and α-SMA to inhibit the process of fibrosis in DN and mediate the phenotypic transformation of glomerular mesangial cells.The phenotypic transformation of glomerular mesangial cells in glucose may also depend on high osmotic pressure in DN.