Objective To study the clinical effect of ceramic cover anterior on teeth beauty.Methods 138 teeth in 34 patients with dental fluorosis were divided into two groups,the control group (69 teeth in 16 patients) was given traditional PFM cover repair,the treatment group (69 teeth in 18 patients) was given ceramic cover,they were followed up for three years.The teeth remaining rate and patient satisfaction were compared,the match color,edge fit,healthy gums,restoration integrity were compared according to the Ryge standard.Results Teeth remaining rate in treatment group was 95.7％,which was significantly higher than control group (P ＜ 0.05).The patient satisfaction was 94.4％ in treatment group and 87.5％ in the control group,the difference was significant (P ＜ 0.05).The match color,edge coloring,healthy gums and teeth sensitivity were not different between the two groups (P ＞ 0.05).The restoration integrity and marginal fit of treatment group was better than control group (P ＜ 0.05).Conclusion The clinical effect of ceramic faced in the anterior teeth beauty repair,especially in the repair of dental fluorosis is remarkable,and the remaining teeth rate higher,repair,dental restoration integrity and marginal fit is significantly better than that of resin repair,patient satisfaction is higher,the water area from non-beryl popularization and application.

Objective: To prepare modern tradional Chinese patent medicine Lemai capsules with a low dosage. Methods: Comparing the applications of seven kinds of macroporous adsorption resin in refinement of Lemai capsules' extracts in danshensu and paeoriflorin determined by HPLC as markers. Results: After refinement, the solid content of extracts decreased greatly. The content of danshensu became much lower than that before refinement The retention rate of AB 8、 X 5 and WLD for paeoriflorin is up to 80%. Conclusion: 7 kinds of macroporous adsorption resin are not proper to danshensu but some of them can be used to paeoriflorin.

AIM: To improve the quantitive method of sarsasapogin in Kangbindu Tablet(Rhizoma Anemarrhenae, Fructus Forsythiae, etc.) METHODS: Applying HPLC-ELSD to replace TLCS. RESULTS: The new method was better than the old one in precise and repeat. CONCLUSION: The new method HPLC-ELSD is more useful.

TCM is unique and e ective on senile dementia in both theory and practice;therefore it is signi cant to generalize TCM understanding and experience on senile dementia for speci c therapy to reduce patients' cognition impairment and enhance their living quality.Sorting of TCM understanding on senile dementia pathogenesis and causes,it was clear that seniledementia was often seen in aging people characterized by de ciency in origin and excess in super ciality.Its de ciency in origin was de ciency of kidney essence,marrow and lucid yang failing to ascend.The origin aspect of senile dementia was believed to be the insu ciency of kidney.The de ciency of ve zang-organs was closely related to it.While the super ciality aspect was thought to be turbid phlegm and stagnant blood blocking brain and cerebral meridian with ve zang-organs all concerned.On one hand,the de ciency of kidney assisted by the de ciency of ve zang-organs could result in phlegm and blood stasis,that is to say excess resulted from de ciency.On the other hand,the stagnation of phlegm and blood stasis could block the generation,transformation and transportation of qi,blood and body uid,which would further consume the essence of the body.This is so-called de ciency transformed from excess.The above two aspects a ected each other and causing lingering pathogenic factors.

Acute abdominal pain is a major clinical symptom of acute abdomen,and vasogenic acute abdomen caused by vascular diseases is rare and difficult to diagnose at its early stage with atypical clinical symptom and high mortality.Therefore,there is an urgent need to strengthen and improve the knowledge of surgeons about vasogenic acute abdomen.

AIM: To observe the effects of hypertriglyceridemia and fenofibrate on CD40L expression in platelets in vitro and in vivo. METHODS: In vivo experiments, according to its own strict standards, 20 patients were respectively selected for hypertriglyceridemia group and control group, before and after treatment of fenofibrate for hypertriglyceridemia patients. The CD40 ligand positive rates of platelets by flow cytometry and plasma soluble CD40 ligand by ELISA were examined under the same conditions as control group. The CD40L and sCD40L in each group were compared. In in vitro experiments, all 6 objects plasma was chosen in the same condition except for triglyceridemia, after the co-incubation of these plasma with the same healthy platelets was performed and the interference with wy14643, the CD40 ligand positive rate of platelets by flow cytometry and total platelets CD40 ligand protein content by Western blotting were examined under the same conditions in all objects. The CD40L positive rate and total CD40L content in each group were compared, respectively. RESULTS: The platelet CD40L positive rate and plasma sCD40L concentration in hypertriglyceridemia group were significant higher than those in control group (P<0.01). Followed the TG concentration decreased, the platelet CD40L positive rate and plasma sCD40L concentration decreased after the treatment of fenofibrate, the same as the total platelets CD40L content which was significant higher in hypertriglyceridemia group than that in control group in vitro (P<0.05). No effect of wy14643 on the total CD40L content expression was observed in vitro. CONCLUSION: Hypertriglyceridemia plasma stimulates immune-activation of platelets both in vitro and in vivo. sCD40L may mainly come from CD40L on platelet membrane. PPARα activator of fenofibrate may inhibit the immune-activation of platelets by reducing the concentration of plasma TG, but PPARα activator WY14643 cant inhibit the expression of CD40L and CD40L in vitro.

Objective To observe the outcomes of satellite ganglion block(SGB)via posterior edge of stcmoclcidomastoid approach.Methods SGB was performed in 2 400 patients,who wererandomly divided into two groups with l 200 cases each.The puncture of SGB in group SPA was via posterior edge of sternocleidomastoid approach and that in group TPA via traditional paratracheal approac}L The same local anesthetics was used in two groups.The success rate(Horner'S syndrome appearance)and complications were compared.Results The success rate was higher in group SPA than that in group TPA(97% VS.92%)(P<0.05).Repeated puncture was needed in 16 cases ingroup SPA,which were less than 98 cases in group TPA(P<0.05).Laryngeal nerve block wasoccurred in 24 cases in group SPA.which were less than 37 cases in group TPA(P<0.05).Conclusion Compared to the traditional paratracheal approach,SGB via posterior edge ofsternocleidomastoid approach has the advantages of clear positioning,easy puncture,higher success rate and less complications.

OBJECTIVE To study the classification the ?-lactamases produced by Providencia spp.METHODS To detect the ?-lactamases produced by 19 strains of Providencia spp with nitrocefin test,a multi-disk test(synergy test,antagonized test for the inducible AmpC ?-lactamases(IABLs),AmpC ?-lactamases(ABLs) phenotype test and extended-spectrum ?-lactamases(ESBLs) screening test,carbapenem-hydrolyzing ?-lactamases(CHBLs).RESULTS The nitrocefin tests of all of 19 strains were positive,producing the ?-lactamases(100.0%);eight strains produced broad-spectrum ?-lactamases(BSBLs,42.1%) and ESBLs(42.1%),respectively,one strain produced overexpression of AmpC,3 strains produced CHBLs.CONCLUSIONS The rate of ?-lactamases produced by the 19 strains of Providencia spp is very high,there are three kinds of ?-lactamases,mainly BSBLs and ESBLs.

Objective To compare local infiltration anesthesia with different concentrations of ropivacaine for postoperative analgesia in pediatric patients undergoing tonsillectomy.Methods Sixty ASA physical status Ⅰ patients of both sexes,aged 6-12 yr,weighing 18-41 kg,scheduled for elective tonsillectomy,were randomly divided into 3 groups (n =20 each) using a random number table:control group (group C); 0.2％ ropivacaine group (group R1) ; 0.5 ％ ropivacaine group (group R2).Anesthesia was induced with inhalation of sevoflurane,injection of sufentanil and cisatracurium and maintained with inhalation of sevoflurane and iv infusion of remifentanil.The tonsil was locally infiltrated vith 0.2 ％ and 0.5％ ropivacaine (3-5 ml on each side of the tonsil) before surgery in R1 and R2 groups,respectively.Tonsillectomy was performed under general anesthesia.At 1,3,6,12,and 24 h after surgery,pain was assessed using faces pain scale-revised (FPS-R).Paracetamol 5 mg/kg was used as rescue analgesic when FPS-R scores ≥4.The interval between awake extubation and the first request for analgesic,requirement for postoperative analgesic and development of adverse effects were recorded.Results Compared with group C,the interval between awake extubation and the first request for analgesic was significantly prolonged,and the requirement for postoperative analgesic was decreased in R1 and R2 groups (P ＜ 0.05).Compared with group R1,the interval between awake extubation and the first request for analgesic was significantly prolonged,and the requirement for postoperative analgesic was decreased in R2 group (P ＜ 0.05).There was no significant difference in the incidence of adverse effects between the two groups (P ＞ 0.05).Conclusion The optinum concentration of locally infiltrated ropivacaine is 0.5 ％ for postoperative analgesia in pediatric patients undergoing tonsillectomy.

Objective To evaluate the role of angiotensin Ⅱ receptors in the development of neuropathic pain (NP) in rats.Methods Seventy-five male Sprague-Dawley rats,weighing 230-270 g,were randomly divided into 5 groups (n =15 each) using a random number table:sham operation group (group S),group NP,telmisartan (angiotensin Ⅱ receptor antagonist) 2 mg group (group T1),telmisartan 5 mg group (group T2),and telmisartan 10 mg group (group T3).NP was induced by chronic constrictive injury (CCI) in chloral hydrateanesthetized rats.The sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread.In S group,the sciatic nerves were exposed but not ligated.Telmisartan 2,5 and 10 mg/kg were perfused via stomach once a day for 14 consecutive days in T1,T2 and T3 groups,respectively,while the equal volume of normal saline was given instead of telmisartan in S and NP groups.Mechanical and thermal pain thresholds were measured at 1 day before CCI and 3,7 and 14 days after CCI in each group.Results The mechanical and thermal pain thresholds were significantly decreased after CCI in NP,T1,T2 and T3 groups as compared with group S.The mechanical and thermal pain thresholds were significantly increased after CCI in T1,T2 and T3 groups as compared with group NP.The mechanical and thermal pain thresholds were significantly higher after CCI in T2 and T3 groups than in group T1.There was no significant difference in the mechanical and thermal pain thresholds between T2 and T3 groups.Conclusion Angiotensin Ⅱ receptors are involved in the development of NP in rats.