Objective To analyze clinical Value of serum homocysteine(Hcy)and cystatin C(Cys C) in patients with cerebral in‐farction .Methods From August 2010 to December 2014 ,our hospital 118 patients with cerebral infarction and 62 healthy subjects for the study were divided into the control group and the observation group .The observation group was further divided into light , medium and heavy groups .Test results for the two groups were related to the study of serum homocysteine and Cys C were ana‐lyzed separately and compared between two groups of Hcy levels Cys C contact with cerebral infarction .Results Serum Hcy ,Cys C levels significantly higher in the observation group than that of control group ,differences were statistically significant(P<0 .05);The serum Hcy ,Cys C of patients with severe cerebral infarction group were significantly higher than that of light and medium cer‐ebral infarction group;Serum Hcy ,Cys C levels of patients with medium cerebral infarction group were also significantly higher than the light cerebral infarction group ,differences were statistically significant(P<0 .05) .Conclusion The serum Hcy and Cys C has important clinical value of detecting the disease assessment and treatment of patients with cerebral infarction .

Objective To investigate the process of Stark cesarean section (CS) and analyze its key procedures and patients outcomes in order to understand its advantages and promote its standardization. Methods Elective Stark CS cases were divided into two groups according to time sequence and procedure difference. Group A refered to cases underwent modified approaches before standardization (n=362), and group B refered to cases afterward (n= 302). Duration of operation, time interval from incision to delivery, intraoperative hemorrhage, postoperative flatus time, postoperative morbidity, duration of retained urinary catheter, urinary tract irritation, wound infection, and abdominopelvie adhesion in the second operation were compared between the two groups. Results The average duration of the operations and time interval from incision to delivery in group B, which were (27.7± 10. 8) min and (4.92±1.21) min, respectively, were significantly shorter than those in group A, which were (35.6±15.2) min and (7.81±2. 79) min, respectively (P<0. 05). No significant differences was found with regard to average intraoperative hemorrhage between group A and B [(214.34±62. 1) ml vs (201.54-53.1) ml, P>0.05]. Postoperative flatus time in group B was significantly shorter than that in group A [(16.85±11.8) h vs (25.9±12. 7) h, P<0. 05]. Postoperative morbidity in group B was significantly lower than that in group A [1.3%(4/302) vs 4.7%(17/362), P<0.05]. The average duration of retained urinary catheter in group B was significantly shorter than that in group A [(15.6±5.3)h vs (26. 2±6.1)h, P<0. 05], and the urinary tract irritation rate in group B was also lower than that in group A [2. 5%(9/362) vs 0(0/302), P < 0. 05)], No incisional infection or delayed healing was found in either group. Rectus muscles, fascia and peritoneum adhesions were found in three cases with second surgery in group A and none in group B. No omentum, peritoneum and visceral peritoneum adhesion was found in either group. Conclusions Compared to the modified Stark operation, standardized procedure decreases operation associated complications and improves maternal outcomes. Therefore, standardized Stark CS should be promoted for better operative outcomes.

Clinical teaching and studying is suffering increasing hardship.This article analysises the present condition of clinical education problems and causes,and researches whether patients are obliged to coorperate clinical teaching and studying when they protect their rights.It also researches how to practice clinical teaching and studying in educational hospital and looks for effective pathway to go out of the clinical education problem.

Objective: To explore diagnostic value of dynamic electrocardiogram (DCG) on coronary artery disease. Methods: A total of 100 inpatients with “angina pectoris” history from our hospital were enrolled and received 24h DCG measurement. The DCG examination results were compared with those of coronary angiography (CAG) and analyzed then. Results: Among the 100 patients, there were 85 patients with positive DCG, including 70 cases with positive CAG and 15 cases with negative CAG; and 15 patients with normal (negative) DCG, including 10 cases with negative CAG and five cases with positive CAG. With CAG as golden standard, sensitivity, specificity, positive predictive value (accuracy) and negative predictive value of DCG in diagnosis of coronary heart disease were 93.3%, 40.0%, 82.4% and 66.7% respectively. Partial patients had “angina pectoris”, no stenosis in CAG but there’s slow blood flow. Conclusion: Dynamic electrocardiography is one of important measuring methods diagnosing coronary heart disease. It is simple, practical, safe, economic and appropriate for wide application in clinic, but its specificity is just 40.0%, so coronary heart disease cannot be excluded in negative patients.

Objective To analyse the efficacy of hysteroscopic resection and norethisterone in abnormal uterine bleeding.Methods 38 cases of submucos myoma of uterine body,26 cases of endometrium polyps and 16 cases of complex hyperplasis of endometrium were treated with hysteroscopic resection,and all the cases take norethisterone for one month before hysteroscopic resection and go on taking norethisterone for three months after operation.All the cases were followed up with the improvement of symptoms and the chang of uterine cavity.Results All the cases were followed up for 3～18 months after operation.The efficacy of submucous myoma of uterine body was 92.10%(36/38) and that of endometrium polyps was 84.61%(22/26) and that of complex hyperplasis of endometrium was 93.75%(15/16).Conclusion The treatment of hysteroscopic resection and norethisterone in submucous myoma,endometrium polyps and complex hyperplasis has advantages of satisfied results,slight trauma,fast recovery and fewer recurrence relapse.

Objective In order to elucidate the mechanism of migrating motor complex (MMC) in prophylaxis and treatment of cholelithiasis,the relationship and interaction between MMC and the bile duct motility were investigated. Methods Forty five adult guinea pigs were allocated into three groups with 15 each. The guinea pigs in control,prucalopride and dibazol groups were fed with saline (0.15 ml/100 g),prucalopride (0.01 mg/100 g) or dibazol (0.6 mg/100 g),respectively. The myoelectrical activities of antrum,duodenum,gallbladder(GB) and sphincter of Oddi(SO) of guinea pigs were recorded simultaneously before and after administration via implanted silver electrodes of the muscle layer. Results Positive correlation in frequency and strength of spontaneous myoelectrical activity was demonstrated among antrum,duodenum,GB and SO before administration( P

Objective To investigate the changes of stress responses and fibrinolysis under three anesthesia techniques undergoing elective hysterectomy during perioperative period. Methods Thirty ASA Ⅰ or Ⅱ patients aged 31-57yr weighing 45-70 kg undergoing elective hysterectomy were divided randomly into 3 groups ( n = 10 each): group Ⅰ continuous epidural anesthesia (E); group Ⅱ general anesthesia combined with continuous epidural anesthesia (G + E) and group Ⅲ general anesthesia (G) . The patients in the 3 groups were all premedicated with midazolam 0.05 mg?kg-1 i.v. . In group G and G + E the patients received an additional scopolamine 0.3 mg i.v. . In group E and G + E an epidural catheter was placed through L1-2 interspace and 2% lidocaine 5 ml was given as test dose. The spinal segments blocked was extended from L3 to T6 using 0.75 % bupivacaine. In group G and G + E general anesthesia was induced with fentanyl 2-3 ?g ? kg-1, propofol 1.5-2.0 mg?kg-1 and vecuronium 0.1 mg?kg-1 and maintained with isoflurane inhalation and intermittent i.v. boluses of vecuronium. The patients were mechanically ventilated after tracheal intubation. BIS, ECG, BP, HR, SpO2 , PETCO2 and end-tidal isoflurane concentration were continuously monitored during operation. BIS value was maintained below 60. PCEA or PCIA was started as soon as the patients were awake. VAS scores were maintained below 3-4. Venous blood samples were taken before anesthesia (T0 baseline) at the end of operation (T1) and 24 and 72 h after operation (T2 , T3) for determination of plasma adrenaline (A) and noradrenaline (NA), D-dimer level (DD), tissue type plasminogen activator (t-PA ) and plasminogen activator inhibitor ( PAI-1) concentrations. Results The plasma NA and A concentrations in group G were significantly increased at T1-3 as compared to the baseline value at T0 and were significantly higher than those in group E and G + E. There was no significant difference in plasma NA concentration between group E and G + E. The DD level was significantly increased at T2 as compared to the baseline value at T0 in all 3 groups and remained high at T3 in group E and G + E. The t-PA and PAI-1 activities were significantly increased at T1 as compared to the baseline values at T0 in all three groups and then gradually decreasing at T2 and T3 . In group E the PAI-1 activity returned to baseline level at T3 . Conclusion Epidural anesthesia helps in maintaining fibrinolytic activity by attenuating the responses to surgical stress and inhibiting the increase in PAI-1 activity.

AIM: To establish a RP-HPLC for determing the contents of naringin,hesperidin and baicalin in Qingfei Huatan Pill(Arisaema Cumbile,Fructus Aurantii,Pericarpium Citrireticulatae,Radix Scutellariae,etc.). METHODS: Naringin,hesperidin and baicalin were separated on a RP C_(18) column.Acetonitrile-water(20(∶)80)(adjusted to pH 2.2 with phosphoric acid) was used as the mobile phase.The detective wavelength was set at 280 nm. RESULTS: The linear ranges were in 1.24-6.2 ?g for naringin (r=(0.999 7))、1.1-5.5 ?g for hesperidin(r=(0.999 8)) and 2.32-11.6 ?g for baicalin(r=(0.999 9)).The recoveries were 99.29% with RSD of 1.78% for naringin、100.08% with RSD of 1.44% for hesperidin and 98.76% with RSD of 1.42% for baicalin respectively. CONCLUSION: The method is simple,accurate and reproducible.It may well be used for the quality control of this product.

Objective: To work out a screening checklist for childhood autism and explore its reliability and validity. Methods: Items were selected based on the knowledge of clinical manifestation and diagnostic criteria of childhood autism. The autism diagnostic interview-revised version was used to refine the draft. Three groups (childhood autism, mental retardation and normal control) were used to test the reliability and validity of the new screening tool. Results: The checklist had satisfying reliability including good inter-rater reliability, test-retest reliability, split half reliability and internal consistency. The differential validity of the checklist and the differential diagnostic validity of the items were good, so as the construct validity and the criterion validity. The cut-off point for screening and diagnosis were 21 and 24 respectively. Conclusion: The childhood autism checklist has good reliability and validity, and fit for the situation of Chinese children with the disorder.

Objective To investigate the clinical value of laparoscopic treatment of myoma in the broad uterine ligament.Methods A retrospective comparison was made on clinical data between 26 patients who had received laparoscopic enucleation of myoma in the broad uterine ligament and 12 patients who had received traditional open resection in the same period.Results Both laparoscopic and traditional operations were successfully completed.As compared with open surgery,the laparoscopic surgery had longer operative time(92.5?15.8 min vs 63.0?15.8 min;t=5.350,P=0.000),similar blood loss during operation(99.2?32.9 m1 vs 91.7?30.7 m1; t=0.667,P=0.509),and shorter time of postoperative hospital stay(4.5?0.7 d vs 7.7?0.9 d;t=-11.960,P=0.000),respectively.Conclusions Laparoscopic enucleation of myoma in the broad uterine ligament is safe and effective and offers a rapid postoperative recovery.