AIM:To develope a reversed-phase high performance liquid chromatographic method for determing chlorogenic acid and caffeic acid in Fanhuncao Granule(Senecio cannabifolius Less). METHODS: The operation was carried out on Eclipse XDB-C_(18) column with the mobile phase consisting of a mixture of acetonitrile-0.4% phosphoric acid(12(∶)88,V/V).The flow rate of 1.0 mL/min and UV detection wavelength at 327 nm were set to determine the contents of chlorogenic acid and caffieic acid. RESULTS: There was good linear relationship between the concentrations and the peak-areas of chlorogenic acid and caffeic acid.The two kinds of standard solutions were both stable in 16 h(RSD=1.55% for chlorogenic acid,1.11% for caffeic acid) The average recovery was 100.1% and 99.8% for chlorogenic acid and caffeic acid,repectively. CONCLUSION: The method is simple,sensitive,rapid and accurate,and can be used for the quality control of Fanhuncao Granule.

Objective To analyze the overseas imported malaria situation of Shijiazhuang City from 2012 to 2015,so as to provide the evidence for exploring the prevention and control strategy. Methods The data of overseas imported malaria were collected and analyzed with the descriptive method including Plasmodium species,population characteristics,epidemic focus distribution,onset time,diagnosis and treatment in Shijiazhuang City from 2012 to 2015,and the time from the onset to first medical visit,time from first medical visit to being diagnosed,and time from onset to being diagnosed of different Plasmodium species were analyzed respectively with the statistical analysis method. Results A total of 92 overseas imported malaria cases were reported in Shijiazhuang City from 2012 to 2015,including 88 cases from African countries(falciparum malaria taking 53.41％),and 4 cases from Southeast Asian and other countries(vivax malaria taking 50％). Eighty-nine cases were distributed in 28 counties(districts)of 6 cities in Hebei Province,except 2 persons with foreign nationalities and 1 with Anhui Province cen-sus register. The male and young adults were dominant. The median time from the onset to seeing a doctor was one day and the median time from seeing a doctor to being diagnosed was five days. Most cases were reported by the Fifth Hospital of Shijia-zhuang which was the sentinel hospital. Totally 42.39％of the cases were misdiagnosed when the first visit to a doctor. All of the cases were laboratory confirmed and 100％of them received the standard treatment after diagnosis. Conclusions The overseas imported malaria cases are increasing rapidly with years and the malignant malaria cases were more than other malaria cases in Shijiazhuang City. It is necessary to further strengthen the long-term cooperation mechanism between the medical institutions and the entry-exit inspection and quarantine department. The technician training should be strengthened to avoid the severe cas-es and death cases.

Objective:[WT5BZ]The effect of different contents of zinc in feed on learning and memory function and brain somatostatin,zinc and calcium concentrations in rats were studied. [WT5HZ]Methods:[WT5BZ]The feeds contained 20, 50, 100, 200, 400, 800 mg/kg zinc respectively.The learning and memory function of animals were determined by radioimmunoassay.Their zinc and calcium contents were determined by atomic absorption spectrophotometry. [WT5HZ]Results:[WT5BZ]Feeds containing 100 and 200 mg/kg zinc were adequate for learning and memory function of rats,whereas other feeds resulted in poor learning and memory function,and depression in somatostatin concentration in hypothalamus,hippocampus and cerebrum cortex.Additionally,feeds containing lower than 100 mg/kg zinc decreased zinc concentrations in blood,hippocampus,cerebrum cortex and hypothalamus,and calcium concentration in cerebrum cortex. [WT5HZ]Conclusion:[WT5BZ]These results suggest that 100 200 mg/kg zinc in feed is adequate for maintaining learning and memory function in rats.

Objective: Three experiments were used to evaluate the effects of zinc deficiency on growth, learning and memory ability of rats. [WT5FZ]Methods: In experiment Ⅰ and experiment Ⅱ, the experimental rats were divided into three groups, which were zinc deficiency group (ZD), pair fed group (PF), and zinc deficiency and supplementation group (ZS). Their durations were 35 days and 28 days respectively. In experiment Ⅲ, the experimental rats were divided into ZD, PF and ad libitum (AL) group. Results: Zinc deficiency significantly decreased the growth and learning ability of rats, whereas zinc supplementation reversed the above status. Conclusion: The results suggest that zinc not only influence growth, but also the advanced function like learning and memory ability of brain.

Objective To explore the magnetic properties of permanent magnet wafers commonly used in magnetotherapy to provide a basis for the scientific and rational use of sources of magnetism.Methods A magnetometer was used to measure the magnetic induction intensities of the N and S polar centers of eight groups of magnet wafers (axial magnetization) with nominal magnetic induction intensities of 6 mT, 10 mT, 20 mT, 30 mT, 60 mT,100 mT, 200 mT and 350 mT.Results There were some differences between the nominal values and the measured mean magnetic induction intensities. In a few groups the differences were close to 20％. There were also differences within the same group. The magnetic induction intensities of the two polar surfaces were not always equal for the same magnet, and there were large differences among individual magnets.Conclusions The intensities of magnetic sources must be measured before use to ensure the consistency of magnetic induction intensities in therapeutic applications.

Objective To understand the status of mycoplasma infection and drug susceptibility to provide reference basis for clinical rational drug use.Methods The reagents kits produced by Shanghai Aopu Biopharmaceutical Co.,Ltd.were adopted to per-form the mycoplasma culture,identification and susceptibility test.Results Among 1 745 specimens,896 cases of mycoplasma in-fection were detected with the total detection rate of 51 .35%,including Ureaplasma urealyticum (Uu)infection in 841 cases (48.19% ),Uu combined Mycoplasma hominis(Mh)infection in 92 cases(5.27% )and Mh infection in 17 cases(0.97%).The de-tection rate was 9.46% in males and 41 .89% in females,the infection rate in females was obviously higher than that of males.The results of drug susceptibility test showed that the sensitive rate to doxycycline,josamycin and minocycline was higher than 95%, while the resistant rate to norfloxacin and lincomycin was higher than 75%.Conclusion Mycoplasma infection in this hospital is dominated by Uu.Doxycycline,josamycin and minocycline can be used as the empirical medication to treat mycoplasma infections in this hospital.But before clinical treatment,the mycoplasma culture and the drug susceptibility test should be performed as possible for rational and standardized medication.

Objective To explore the 5,10-methylenetetrahydrofolate reductase( MTHFR) and 5-methyltet-rahydrofolate-homocysteine methyltransferase reductase( MTRR) gene polymorphisms among the Han women in Laiwu City.Methods A total of 559 Han women were recruited.And their oral epithelial cells were collected to extract genome DNA in order to detect gene polymorphisms of MTHFR and MTRR using fluorescence quantitative PCR.Then the results were compared with those in other cities in China.Results The frequency of MTHFR 677CC,677CT and 677TT of Han women in Laiwu city was 14.3%,46.7%and 38.1%,respectively.The frequency of MTHFR 677TT among Laiwu women was significantly different to those of Zhenjiang, Wuhan, Kunming, Deyang, Huizhou, Qionghai (P<0.05).The frequency of AA,AC,CC gene type on MTHFR A1298C was 78.2%,19.7% and 2.1%,respec-tively, the frequency was significantly different to those of Zhenjiang, Wuhan, Kunming, Deyang, Huizhou, Qionghai (P<0.05).The frequency of AA,AG,GG gene type on MTRR A66G was 53.3%,38.8%and 7.9%,respectively. The frequency of MTRR 66GG was significantly different to that of Qionghai(P<0.05).Conclusion The MTHFR, MTRR polymorphism distribution of Han women in Laiwu City has the characteristic of region specificity,respectively.

Objective To observe the effects of permanent magnetic fields with different magnetic inductions on oxidative damage to human brain microvascular endothelial cells (HBMECs) under normal and hypoxic conditions. Methods HBMECs were cultured in vitro under normal and hypoxic conditions,then divided into a control group and groups receiving magnetic induction at 8.1,16.5,20.3,26.0,27.3,62.5,110.7 and 215.6 mT.Changes in superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in each group were measured 72 h after exposure to the magnetic fields. Results Under normal conditions the SOD activities of the magnetic groups were not significantly different from that of the control group. Only the MDA content of the 215.6 mT group was statistically different (slightly higher) than that of the control group (P ＜ 0.05 ).Under hypoxic conditions,there was again no statistically significant difference in SOD activity,but the MDA content of the 110.7 mT group was significantly lower. Conclusions Under normal conditions,a 215.6 mT permanent magnetic field has an effect on MDA in HBMECs; under hypoxic conditions a 110.7 mT permanent magnetic field has such an effect.

Objective To investigate the correlation between hepatitis B virus(HBV)markers and AFU,ALT,GGT.Methods 570 eases of HBV-infected people were divided into 4 groups according to the different performance modes.150 healthy people were considered as control group.rate method was used to detect AFU,ALT and GGT.Estimate the differences between the groups were compared.Results The comparison between the AFU,ALT,GGT in different performance mode of HBV and the control group showed:the differences of group 1,group 2 and group 3 were statistically significant(P＜0.01),the difference of group 4 was not significant(P＞0.01).The correlation between AFU,ALT,GGT of different performance mode showed:the changes of AFU and ALT,GGT in group 1,group 2 and group 3 were positively change-related.But the changes of AFU,ALT and GGT were not clearly related.The distribution of the rise of serum enzyme activity in different performance mode showed:the AFU,ALT and GGT rised in different proportion in group 1,group 2 and group 3.Conclusions The AFU,ALT and GGT activity of HBVinfected persons should be detected at the same time,and evaluate the damage of liver cells.This will do help to predict the progression of hepatitis.

Objective To observe and explore clinical efficacy ofTianma-Gouteng decoction combined valsartan in the treatment of patients with renal hypertension and its effects on renal function. Methods A total of 130 patients with renal hypertension were enrolled and randomly divided into a study group (68 patients) and a control group (62 patients). Both groups were given prescription of lower sodium diet, exercising and enalaprilscheme. On this basis, the control group plus valsartan, and the study group was further added withTianma-Gouteng decoction. After 2 courses treatment, renal function, and blood pressure of both groups were compared, and clinical efficacy on blood pressure were evaluated.Results After treatment, the SBP (126.8 ± 9.1 mmHg vs. 134.1 ± 8.8 mmHg,t=4.648), DBP (82.4± 5.0 mmHgvs. 85.3 ± 5.4 mmHg, t=3.167), Scr (148.5 ± 46.3μmol/Lvs. 172.1 ± 52.0μmol/L, t=2.723), BUN (8.3 ± 2.7 mmol/Lvs. 9.7 ± 3.1 mmol/L,t=2.734) and 24hAlb (1.7 ± 0.6 gvs. 1.9 ± 0.7 g,t=2.209) in the study group were significantly lower than control group (P<0.05 orP<0.01). The total effective rate in the study group was significantly increased than that in the control group (91.2%vs. 79.0%;χ2=0.383,P=0.050).Conclusion Valsartan combined with Tianma-Gouteng decocntion can reduce blood pressure, and alleviate the kidney damage effectively.