1.Application of the NuMED covered CP stent in treatment of adult coarctation of the aorta
Changxue ZHAO ; Yanping SONG ; Rong FENG
Chinese Journal of Interventional Cardiology 2017;25(4):206-209
Objective To evaluate the NuMED covered CP stent for the treatment of adult aortic coarctation.Methods 6 patients with coarctation of the aorta received covered CP stent implantation.There were 4 males and 2 females cases with mean age of (20.13±5.16) years old.The size of the covered CP stents and balloons were determined by preoperative aortic CTA assessment.Stent implantation was per formed through femoral approach and the puncture site was sealed by vascular closure device after the operation.Results All the NuMED covered CP stents were implanted successfully.The pressure gradient dropped from (59.32±15.78) mmHg to (10.83±3.66) mmHg (P<0.001) after stenting.The diameter of the coarcation at the narrowest point increased from preoperative (5.1±1.2) mm to (19.7±2.4) mm after stenting (P<0.001).In 2 cases with PDA, the shunting disappeared spontaneously after operation with normalized of lower limb blood pressure.All the patients were followed up clinically for 12-36 months and all of them reported improvement in clinical symptoms post operation.Follow up Doppler echo or aorta CTA examinations at 3 months showed no restenosis.No mortality recorded during follow up.Conclusions NuMED covered CP stent for the treatment of aortic coarctation in adults showed satispactony short term outcome.Its long term outcome still requires further in vestigation.
2.Quality standard for Kudingcha Tablets
Mei LU ; Yanping RONG ; Daping MENG ; Hualing WEI
Chinese Traditional Patent Medicine 1992;0(03):-
AIM: To establish a quality standard for Kudingcha Tablets(Cratoxylum Prunifolium Dyer). METHODS: TLC was adopted for identification of Kudingcha Tablets.RP-HPLC was used to determine the contents of ursolic acid,quercetin and kaempferol in the preparation.The Nova-Pak C_(18) column(3.9 mm?150 mm,4.0 ?m) was used to determine the ursolic acid of content.The mobile phase was methanol-water-H_3PO_4(85∶15∶0.1).The detection wavelength was at 220 nm.The flow rate was 0.8 mL/min and the temperature was at 30 ℃.The Nova-Pak C_(18) column(3.9 mm?300 mm,4.0 ?m) was used to determine the contents of quercetin and kaempferol.The mobile phase was methanol-0.4%H_3PO_4(50:50).The detection wavelength was at 360 nm.The flow rate was 0.7 mL/min.The temperature was at 40 ℃. RESULTS: A good linear relation was obtained when the sample size of ursolic acid was in the range of 0.334-6.672 ?g.The linear range of quercetin was(0.012 99-0.649 6) ?g and the linear range of kaempferol was 0.013 34-0.667 2 ?g.The average recoveries of ursolic acid,quercetin and kaempferol were 97.70%,96.89% and 97.41%,respectively.The RSD were 0.87%,(1.06%) and 1.40%(n=6),respectively. CONCLUSION: This standard is accurate and reliable,and can effectively control the quality of Kudingcha Tablets.
3.Development and preliminary application of quality evaluation scale for graduation thesis of undergraduate from serf-study examination of nursing
Hong GAO ; Rong WANG ; Gongxiang DUAN ; Yanping WAN
Chinese Journal of Practical Nursing 2013;29(22):5-8
graduate from self-study examination of nursing,the quality evaluation scale for graduation thesis of undergraduate from serf-study examination of nursing was developed.Methods The quality evaluation scales,which involving the scale using for faculty advisers,the scale using for reviewing teachers and the scale using for thesis defense teachers,for graduation thesis of undergraduate from self-study examination of nursing were constructed by adopting literature method,and so on.The scales were evaluated by reliability analysis,validity analysis,correlation analysis and factor analysis.Results The scale using for faculty advisers included 5 first-level indicators and 16 second-level indicators.The scale using for reviewingteachers included 4 first-level indicators and 14 second-level indicators.The scale using for thesis defense teachers included 5 first-level indicators and 14 second-level indicators.Their content validity indexes were respectively 0.909,0.952 and 0.864.Their construct validity cumulative contribution rates of the dimensions factors were respectively 72.77%,66.19% and 63.79%.Their internal consistency reliability coefficients Cronbach α were respectively 0.953,0.810 and 0.926.The correlations between each factor and total score were significant.Conclusions three evaluation scales have good reliability and validity,which could be used to evaluate graduation thesis quality of undergraduate from self-study examination of nursing.
4.Sequence variability of human cytomegalovirus UL143 open reading frame gene in low-passage clinical isolates
Rong HE ; Qiang RUAN ; Ying QI ; Yanping MA ; Yaohua JI
Chinese Journal of Microbiology and Immunology 2009;29(6):491-494
Objective To explore the relationship between ULI43 sequence variability and clini-cal disease. Methods UL143 from samples obtained from suspected congenitally human cytomegalovirus (HCMV) infected symptomatic infants were PCR amplified and sequenced. Results There were not too much sequence variability of UL143 compared with Toledo. But no one was completely identical to Toledo, and all UL143 ORFs were shorter than Toledo for frame-shift. Conclusion HCMV-UL143 existed in moat of low passage isolates and sequences were variable. No obvious linkage was observed between UL143 poly-morphisms and outcome of suspected congenital HCMV infection.
5.Development and application of computer management system for nursing care knowledge
Rong WU ; Changsheng ZHU ; Hongwei FENG ; Yanping TANG
Chinese Journal of Nursing 2010;45(4):323-325
This paper introduced the development and application of a computer management system for nursing care knowledge,which could manage intra-and extra-nursing care knowledge respectively and realized information share,utilization and innovation. It motivated the nurses to study actively,helped them with knowledge accumulation and innovation,elevated nurses' job identification,optimized team culture construction and increased work efficiency.
6.Analysis of adrenoceptor agonist-octopamine in fish sauce by HPLC
Yanping RONG ; Tingting ZHU ; Shunsheng CHEN ; Jianzhong WANG ; Yinghong QU
Chinese Journal of Marine Drugs 1994;0(01):-
Objective To establish a HPLC method for the determination of octopamine in fish sauce.Methods A phenomenes luna C18 column was used.The mobile phase was 0.02?g?mL-1 citric acid-0.02?g?mL-1 sodium dihydrogen phosphate(7∶3,v/v) and detection wavelength was 274 nm.Results The linear range of octopamine was 104.0%,RSD=1.53%.The detection limit was 5.7ng?mL-1.Conclusion This method is simple,rapid and reliable.It could be used for the determination of octopamine and its related substances in fish sauce.The content of octopamine in Engraulis japonicus sauce is 1055?g?mL-1.
7.Studies on separation of octopamine in fish sauce and affects on flavour of fish sauce
Tingting ZHU ; Yanping RONG ; Shunsheng CHEN ; Yinghong QU ; Jianzhong WANG
Chinese Journal of Marine Drugs 1994;0(03):-
Objective To establish a technical process for the separation of octopamine.MethodsTaking the absorptive capacity and elution efficiency of octopamine as indexes,the absorption characteristics and elution parameters of separation with macroporous resin were investigated.Results The static adsorption capacity of H103 type macroporous resin was 2.925mg g-1.The static elution ratio was 98.04%.Conclusion H103 type macroporous resin is effective to separate the octopamine and improve the flavour of fish sauce.
8.A qualitative research on gestational diabetic patients' primary use of real-time blood glucose monitoring
Lifang YANG ; Fei XIAO ; Rong HUANG ; Yanping XU ; Lingli DING
Modern Clinical Nursing 2017;16(7):39-42
Objective To explore the inner feelings of gestational diabetes at their primary use of the real-time blood glucose monitoring. Method Using the qualitative method, 10 gestational diabetic patients monitored their blood glucose using the real-time blood glucose monitoring for the first time and then were interviewed, the acquired results treated with phenomenological analysis. Result The inner feelings of the 10 patients were summarized including 5 themes:worry about the safety of the fetus, fear on alarming at monitoring, different views on four times input of finger blood glucose values, worry about inaccuracy in blood glucose monitoring data, changes in mental conditions and diets. Conclusion Health care workers should provide the gestational diabetic patients with targeted care strategies based on presence of psychological status and knowledge needs so as to improve their compliance, reduce the psychological burden of patients and control their blood glucose level.
9.Study on the safety margin of esphagus in the radical gastrectomy of adenocarcionma of esophagogastric junction
Haining MI ; Xingwen LI ; Rong YANG ; Yanping YANG ; Tao WANG
Clinical Medicine of China 2017;33(4):308-311
Objective To discuss decision of the safety margin of esophagus in the radical gastrectomy of adenocarcionma of esophagogastric junction.Methods The length of esophageal resection margin of intraoperative,postoperative and pathological large slice under the microscope of 50 cases adenocarcionma of esophagogastric junction underwent radical gastrectomy were measured,and the proximal invasive length of esophagus in large pathological slice were also measured,and the relationship between it and pathological factors was analyzed.Determined the minimum safety cut edge distance.Results In 46 of 50 patients,proximal intramural spread of the tumor was observed.The invasive length of tumor from 0.1 cm to 3.5 cm.The invasive length of tumor was <1.0 cm with 11 patients,1.0-1.5 cm with 19 patients,1.5-3.0 cm with 15 patients,in only 1 patient was the distance of spread 3.5 cm.Invasive length of adenocarcionma of esophagogastric junction had a significant difference between the groups in different pathological factors(P<0.001).Conclusion The length of 3.5 cm is the safety margin of esophagus in the radical gastrectomy of adenocarcionma of esophagogastric junction(SiewertⅡ,Ⅲ type) by abdominal incision.
10.Validation Research And Regulation Exploration Of High Fat-introduced Hyperlipidemia Model In Rat
Yanping WANG ; Danhong PENG ; Xiaoqi LIU ; Rong XIE ; Xiantao LI
Chinese Journal of Comparative Medicine 2017;27(1):5-10
Objective To investigate the feasibility of high fat-introduced hyperlipeidemia model in male SD rat and study the time rule of molding .Methods 30 Male adult rats of SD Strain bred in the animal house of the institute were divided into 3 groups after 1 week adaptation , group 1:control group, normal diet;group 2:model 1 group, high fat high cholesterol diet;group 3:model 2 group, high fat high cholesterol diet .The period of experiment was 8 weeks.Food and water intake were measured everyday and body weight were measured every four days .Blood were collected by orbital venous at the end of fourth ,sixth,eighth week to test their serum lipid level .At the end of experiment ,animals were killed to collect liver and aorta tissue for HE stain .Results Compared with control group ,the food intake of model 1 was higher and model 2 was significant lower , water intake of model 2 was significant lower , the ratio of liver/weight of two model groups were significant heavier ,and weight of model groups were higher .High fat diet significantly increased TC levels of model groups at the end of fourth ,sixth week.The level of LDL-c in model 1 group were higher and the HDL-c were lower compared with control group .HE stain showed the livers of control group were regular ,arrangements of the liver cells were trim, dyeing present uniformity .The two model groups showed a large range of hepatocyte fatty change ,a few liver blood sinus were in congestion and infiltrated with inflammatory cells .Aorta HE stain showed no significant change among 3 groups.Conclusions The method of high fat-introduced hyperlipeidemia model in male SD rat is feasible and the model turned out to present hypercholesterolemia with severe fatty liver .On the other hand,levers of serum lipid increased within an increase—inter-adjustment—increase state .In the process of modeling ,how to overcome the symptom of anorexia and the state of cholesterol inter-adjustment in animals is the key to successfully establish hyperlipeidemia model .