OBJECTIVE:To establish a RP-HPLC method for determination of the content of baicaline in Qingkailing Injection,so as to control the quality of this preparation.METHODS:The column was C18.The mobile phase was methanol-acetic acid-water(45∶1∶55).The detection wavelength was 274nm.RESULTS:There was a good linear relationship between the concentration of baicaline and absorption area value in range of 0.25～2.5?g/ml,r=0.9 992.The average recovery was 98.4%(n=5) with RSD=1.5%(n=5).CONCLUSION:The method was highly repeatable and accurate.

OBJECTIVE: To optimize the extraction technology of Huanggan decoction. METHODS: The extraction technology of Huanggan decoction was optimized by L9(33) orthogonal design with the concentration of alcohol, the amount of solvent and the extraction time as factors taking the contents of chlorogenic acid as indexes. RESULTS: The optimal extraction process was as follows: adding six and five-fold of 70% alcohol, extracting for 60 min and 45 min. CONCLUSION: The extraction technology has been proved to be reasonable, simple and feasible, and it provides theory evidence for production.

ObjectiveTo compare the correlation and agreement of international normalized ratio (INR) measured by INRatio coagulometer and standard laboratory venous blood measurement in order to judge the clinical application value of measuring INR by INRatio coagulometer.MethodsOne hundred who received warfarin were chosen randomly.Venous blood and finger capillary blood were taken simultaneously for measurement of INR by laboratory coagulation analyzer SYSMEX CA-7000 and INRatio coagulometer respectively.The correlation and agreement of INR results tested by the two methods were analyzed.Results When INR ≤5,there was a good correlation between INRatio coagulometer and laboratory coagulation analyzer SYSMEX CA-7000 (r =0.898,P =0.000).The mean bias of the two methods was 0.36 and INR measured by INRatio coagulometer were higher than those tested by laboratory coagulation analyzer SYSMEX CA-7000.By Bland-Altman,it showed a good agreement for 91.2％ (62/68) of the difference INR located 95％ the limited of agreement when the mean INR 1.60 - 3.50,and a bad agreement for only 50.0％(1/2) of the difference INR located 95％ the limited of agreement when the mean INR ＞ 3.50.ConclusionsIn the therapeutic range,INR measured by INRatio coagulometer shows a good correlation and agreement with INR measured by the established laboratory method.INRatio coagulometer can be used to monitor INR in the patients received oral anticoagulant therapy.

Objective To study the incidence and risk factors of retinopathy of prematurity (ROP) in a single tertiary center during different times(Phase 1:2009 to 2011;Phase 2:2012 to 2014). Methods From 2009 to 2014, fundus examinations were performed on premature infants admitted to NICU of our hospital. The incidence and risk factors of ROP were compared between the two phases. Results During Phase 1,68 (11. 1% ) cases were diagnosed with ROP among 614 premature infants. During Phase 2, 121 (15. 2% ) cases were diagnosed with ROP among 794 premature infants. The incidences of ROP between the two phases were significantly different (P <0. 05). During Phase 1 and 2, 10 (14. 7% ) infants and 12 (9. 9% ) infants were diagnosed with Grade 3 and above or threshold ROP, respectively. The incidence of Grade 3 and above or threshold ROP between the two phases were also significantly different (P < 0. 05). Logistic analysis demonstrated that gestational age(GA), birth weight (BW), duration of oxygen therapy and mechanical ventilation(MV) were independent risk factors during hase 1, whereas GA, MV and blood transfusion were independent risk factors during Phase 2. Pulmonary surfactant (PS) was protective factor of ROP in both two groups. GA in Phase 2 was significant lower than Phase 1 (P <0. 05), while duration of oxygen therapy, incidence of MV, PS and blood transfusion were higher in Phase 2 ( P < 0. 05) . Conclusions The incidence of ROP has increased. Reducing the incidence of preterm delivery, duration of oxygen therapy, incidence of MV and blood transfusion has important role in preventing ROP. Reducing the use of MV and the oxygen concentrations, appropriate PS therapy and focusing on early ophthalmic screening are important preventing severe ROP.

Objective To summarize the experience of mammotome minimally invasive biopsy in detection of early breast cancer.Methods 1000 cases with breast lesions underwent minimally invasive surgery under imaging guided mammotome.Results By intraoperative frozen section,10 cases of early breast cancer was found,with 3 millimetre as the minimum lesion diameter,among whom 4 cases were intraductal carcinoma,5 cases were infiltrating ductal carcinoma,and 1 case was mixed carcinoma.Axillary lymph node metastasis was found in only 1 case.Conclusions Non-palpable small lesions cant be ignored.Ultrasound-guided mammotome minimally invasive surgery can accurately position and specifically diagnose non-palpable small lesions or multiple lesions,as well as suspicious malignant lesions.Benign lesions can be directly resected by uhrasound-guided mammotome minimally invasive surgery,which is of great value in detection of early breast cancer.

Objective To investigate the effects of salvianolic Acid A and B(Sal A,Sal B)on acute myocardial ischemia of rats.Method The rat model with acute myocardial ischemia was established by coronary ligation and intravenous injection of posterior pituitary.The effect of Sal A 10,5,2.5 mg/kg and Sal B 10 mg/kg on the electrocardiogram of different time points,serum creatine kinase(CK),lactate dehydrogenase(LDH)and myocardial infarction area were observed.Results To the rat model was established by coronary ligation,Sal A 10,5,2.5 mg/kg and Sal B 10 mg/kg could lower the ST-segment elevation,reduce infarct size and serum CPK,LDH.The effect of Sal A 10,5 mg/kg was significantly higher than that of Sal B.The role of Sal A 2.5 mg/kg was equivalent to Sal B 10 mg/kg.To the rat model was established by intravenous injection of posterior pituitary,Sal A 10,5,2.5 mg/kg and Sal B 10 mg/kg could lower the ST-segment elevation.The effect of Sal A 10,5 mg/kg was significantly higher than that of Sal B.The role of Sal A 2.5 mg/kg and sal B 10 mg/kg had no significant difference.Conclusion Treatment with Sal A 10,5 mg/kg was more effective in the rat models with acute myocardial ischemia than Sal B 10 mg/kg.Sal A 2.5 mg/kg improved acute myocardial ischemia in rats was equivalent to Sal B 10 mg/kg.

OBJECTIVE To study the feasibility of no-hair-shaving before knee arthroscopy surgery.METHODS Totally 160 patients preparing for knee arthroscopy surgery were divided into both test and control groups randomly.The test group was no-hair-shaving before operation,and the control group was hair-shaving the day before their operation.Patients of the test group washed their skin with soap water or bathed at the operation day and patients in control group did this the day before operation.Bacterial culture was done in the operative site both before and after sterilization,and observed whether infection occurred in the incision after operation.RESULTS Bacterial culture in skin before sterilization was statistical significance between the two groups and no statistical significance after sterilization.All the incision healed well and no infection was found.CONCLUSIONS No-hair-shaving preoperatively can decrease patients′ discomfort and reduce colonies on skin surface effectively.The method is easy and safety manipulated.

Objective To investigate the effect of docetaxel combined capecitabine chemotherapy and psychological intervention after radical mastectomy. Methods 80 cases of radical mastectomy for breast cancer treated in our hospital from January 2015 to January 2017 were treated with docetaxel combined with capecitabine chemotherapy. According to different nursing methods, the patients were divided into 2 groups. The observation group was treated with psychological nursing, while the control group received routine nursing care. The anxiety and depression scores in two groups before and after nursing were observed and compared. Results There were no significant difference in the scores of anxiety and depression between the two groups before and after treatment. After the intervention group, patients in observation group were significantly improved, and better than the control group patients; The nursing satisfaction rate in the observation group was 97.5%,which was significantly higher than that in the control group (72.5%) (P<0.05). Conclusion After radical mastectomy of breast cancer with docetaxel combined with capecitabine chemotherapy and psychological clinical observation of dry pre found in breast cancer resection and chemotherapy as the basis, to patients with psychological intervention can improve the clinical situation of patients, promote their mental status improved, improve nursing satisfaction rate of patients, so it is worthy of reference.

Objective To investigate the mechanism of the apoptotic effect of brucine on human multiple myeloma. Methods MTT, morphology, flow cytometry, and RT-PCR were used to observe the apoptotic pathways of brucine on human multiple myeloma cell line-U266. Results The apoptotic effect of brucine showed a dose and time dependent manner, 48 hour IC50 0.16 mg/ml. The cells treated with brucine showed significant feature associated with apoptosis by Hoechst 33258 at fluorescence microscope.Mitochondrial membrance potential showed no statistical significant difference in different concentration with Rhodamine 123 by flow cytometry (P ＞0.05). The Caspase-3 expression detected by RT-PCR was increased at 12, 24 and 48 hours treated with brucine, and its gray value was (0.2597±-0.020), (0.5488±0.016), (0.6205±0.006), (0.6533±0.009) (P ＞ 0.05), detection of added z-IETD-fmk, z-LEHD-fmk (Caspase-8 and Caspase-9specific inhibitor) the expression of Caspase-3, the gray scale values were (0.7118±0.006), (0.2637±0.003)(P ＜0.01); and (0.7182±0.004) (0.7195±0.003) (P=0.836). Caspase-8 was activated. Conclusion Within the 0.4 mg/ml concentration brucine can induce apoptosis in U266 cells. Brucine induced apoptosis on cell line U266 through Caspase-8 activation by death-receptor pathway.