In this essay,the market faults in health-care market are analyzed,which leads to a conclusion that the market relies strongly upon ethics,a kind of internal institution of a society,and hence,reveals the necessity of moral education for medical students.The cultivation of medical ethics among medical students is to meet the joint needs of social justice,development of the profession and benefits of every individual in it.

Objective To observe the clinical efficacy of LiQiDingChuan pill combined with Dexamethasone on the treatment of patients with acute asthma.Methods 93 patients with acute asthma from July 2015 to September 2016 were randomly divided into the observation group(47 cases)and the control group(46 cases).The control group were received dexamethasone, and the observation group were given LiQiDingChuan pill combined with Dexamethasone.The main symptoms disappeared time, forced expiratory volume in 1 second, peak expiratory flow rate value, hs-CRP, TNF-α and clinical efficacy were recorded and analyzed.Results ①After treatment, wheezing cough, chest tightness and wheezing disappeared time were(4.71±0.59)d,(2.85±0.49)d,(4.93±0.70)d in the observation group, which were shorter than(7.76±1.02)d,(5.16±0.74)d,(8.67±1.12)d in the control group, and the differences were statistically significant(P<0.05).② After treatment, FEV1 and PEF levels were(65.69±3.74)V/L,(218.92±16.09)L/s, which were better than(57.45±3.62)V/L,(176.34±15.14)L/s in the control group, and the differences were statistically significant(P<0.05).③ After treatment, hs-CRP and TNF-α levels were(6.76±0.78)mg/L,(7.99±1.87)pg/mL, which were better than(6.76±0.78)mg/L,(7.99±1.87)pg/Ml in the control group, and the differences were statistically significant(P<0.05).④The total effective rate in the observation group was 91.49%, which was significantly higher than 71.74% in the control group, and the differences were statistically significant(P<0.05).Conclusion Liqidingchuan pills combined with dexamethasone treatment has a good therapeutic effect on acute phase of asthma, which can reduce asthma related symptoms, reduce the inflammation level in the patients, optimize the forced expiratory volume in 1 second and peak expiratory flow rate.The combination is better than the single drug.

Pharmaco kinetics of probimane in rabbits was studied by using high-performance liguid chromatography. After the oral adiminis-tration of probimane 75mg/kg to rabbits,the phatmacokinetic characteristics are found to fit a two compartment open model. The pharmacokinetic parameters are: tl/2? = 0.1933h, tl/2? = 4.9568h, K21 =2.9410h-1, K10=0.6492h-1 K12=3.75l2h-1 AUC = 98.9l01h ? mg/L, Cls=0.7583L? kg-1 ? h-1, V/f(c) = 1.0924L ? kg-1, VSS=1.5114L. kg-1.

Objective To prepare monoclonal antibodies against human leukocyte immunoglobulin like receptor subfamilyB2(LILRB2) by hybridoma technique and preliminarily identify their activity in vitro, so as to provide a new strategy for targeting“cold”tumor therapy.MethodsThe extracellular region of human LILRB2 protein was expressed, purified by affinity chro-matography, and then used to immunize BALB/c mice to prepare anti-LILRB2 monoclonal antibodies with high specificityand affinity. The variable regions of light chain and heavy chain of mouse monoclonal antibodies were inserted into an expres-sion vector containing human constant regions by DNA recombination technique to produce chimeric antibodies, and theactivity in vitro was identified by ELISA, flow cytometry and bio-layer interferometry(BLI).ResultsSeven anti-LILRB2monoclonal antibodies with high purity were successfully prepared by hybridoma technique, all of which could recognizeLILRB2 protein with high specificity. Among them, 11C6-8, 43H7-2 and 53A6-11 had superior performance, with the EC_(50)values of 0. 272 3, 0. 431 8 and 0. 344 0 μg/mL, respectively. The chimeric antibodies obtained by humanized modificationexhibited higher binding ability to LILRB2 and effectively blocked the binding of LILRB2 to its ligand, human leukocyteantigen-G(HLA-G), among which the IC_(50)values of 11C6-8 and 53A6-11 were 0. 429 2 and 0. 283 6 μg/mL.ConclusionThe specific monoclonal antibodies against LILRB2 were successfully prepared, and expected to be developed into new anti-tumor immune antibody drugs, which provides a new idea for the development of therapy strategy targeting tumors.

Objective To investigate the effect of plant estrogen on serum hormone levels, NOS and oxidative stress products in patients with uterine fibroids with iron deficiency anemia.Methods 80 cases of hysteromyoma patientswith uterine fibroids with iron deficiency anemia were selected, according to the different medication were randomly divided into experiment group and control group,40 cases in each group.Two groups were treated with anemia correction , mifepristone oral ,and the experiment group were treated on the base of the control group with plant estrogen, 12 weeks for a course.serum sex hormone, NOS, SOD and MDA level were detected.ResuIts Compared with the control group, the indexes of patients in the experimental group were lower(P<0.05), level of serum NOS were higher(P<0.05), level of serum SOD were higher(P<0.05), level of serum MDA were lower ( P<0.05 ) .ConcIusion Plant estrogen can improve the serum levels of serum hormone, NOS and oxidative stress levels of the treatment of uterine fibroids, worthy of clinical promotion.

In 2012, the contracted service with the general practitioners ( rural doctors) was implemented in Zhejiang Province. This paper conducted an analysis on the existing problems in the contracted service from three as-pects of policy background, policy objective, main measures and the following suggestions have been put forward:Strengthening the whole family team of doctors and information construction, and improving the multi sectoral coopera-tion mechanism in order to establish the classified diagnosis and treatment system as targeted to lay a good foundation.

Objective To study the expression and significance of fatty acid synthase ligand ( sFas ) and soluble fatty acid synthase receptor (sFasL) on the neoadjuvant chemotherapy of breast cancer.Methods 84 cases of patients with breast cancer neoadjuvant chemotherapy were recorded from January 2015 to January 2016 in our hospital as breast cancer group,another 84 cases of healthy subjects as control group,the two groups were measured sFas,the positive rate of sFasL.At the same time,respectively before and after neoadjuvant chemotherapy, detection of patients serum sFas, sFasL levels and breast cancer.According to the clinical curative effect were divided into effective group and ineffective group , compared two groups of serum sFas, sFasL levels.Results Breast cancer group called sFas and sFasL positive rate was 58.33% (49/84) and 100.00%(84/84),were significantly higher than that of control group 38.10%(32/84) and 0.00% (0/84);The level of sFas in patients with neoadjuvant chemotherapy was significantly lower than that of neoadjuvant chemotherapy, and the level of sFas in neoadjuvant chemotherapy group was significantly higher than that in ineffective group, the difference was statistically significant (P<0.05).Conclusion In the course of neoadjuvant chemotherapy for breast cancer,the efficacy of neoadjuvant chemotherapy can be effectively detected by detecting the level of serum sFas in patients with breast cancer , while sFasL has no significant change in the neoadjuvant chemotherapy of breast cancer.

Objective To study nuclear factor E-2 related factor 2(NrF-2) and heme oxygenase -1(Ho-1) expression and to explore its clinical significance.Methods From February 2016 to June 2016, 176 cases human gastric tissue paraffin blocks selected in department of pathology in Hangzhou Cancer Hospital, including 78 cases of gastric cancer, 78 cases of normal tissue were analyzed.And the expressions of NrF-2 and Ho-1 were detected by immunohistochemistry in two groups of human gastric tissue.Results The levels of NrF-2 and Ho-1 in gastric cancer group were(0.752 ± 0.098),(0.862 ±0.081) were significantly higher than those in normal group(0.381 ±0.068),(0.412 ±0.083);NrF-2, the positive expression rate of Ho-1 in gastric wall invasion degree of T3 +T4 with lymph node metastasis, NTM stage III and IV gastric cancer tissues were significantly increased.The differences were statistically significant ( P <0.05 ) .Conclusion NrF-2 and Ho-1 play the important role in the development of gastric cancer and NrF-2, the positive expression rate of Ho-1 in the gastric carcinoma and gastric wall invasion, lymph node metastasis and NTM staging are closely related.The clinical diagnosis if the combined detection of NrF-2 and Ho-1, is conducive to the early diagnosis of gastric cancer and the prognosis of the disease.

AIM: To observe the effects of ?-MSH on the expression of CD14 and TLR4 mRNA in mou se peritoneal macrophages induced by LPS and explore the mechanisms of ?-MSH ag ainst LPS. METHODS: BALB/C mouse peritoneal macrophages were cultured in the presence of LPS or LPS plus ?-MSH, and the expressions of CD14 and TLR4 mRNA were detected with the m ethod of reverse transcription polymerase chain reaction (RT-PCR). RESULTS: It was found that native murine macrophages o nly expressed a small amount of CD14 and TLR4 mRNA. Both CD14 and TLR4 mRNA expr ession to LPS in mouse macrophages increased significantly than those of contro l group at 6 h and maintained high level until 24 h when they reached to the pea k. Then the expression of CD14 mRNA backed to the normal gene expression baselin e, while TLR4 mRNA had excessive expression at 48 h. In presence of LPS and ?- MSH, the expression of CD14 and TLR4 mRNA decreased remarkably than those of L PS group (P0.05). Only when the dose s of ?-MSH attained to 1, 10 or 100 nmol/L,?-MSH could affect LPS-stimulated e xpression of CD14 and TLR4 mRNA (P0.05). CONCLUSION: These studies suggest that effects of ?-MSH against LPS are associated with its suppressing the critical receptor (CD14 and TLR4) expression of the LPS signal transduction and inhibiting the activation of macrophages.

Objective To observe the clinical contraeeption effects of GyneFix IN IUD,MCu IUD and TCu220C IUD.Methods From January 2005 to December 2006,comparative study was carried out by randomly applying 3 different types of IUDs(GyneFix IN,MCu and TCu220C IUD) for 300 healthy women.Follow-up survey was taken after 1,3,6,and 12 months.Results The pregnancy rates with IUD among 3 types of IUDs showed no significant difference(P>0.05).The expulsion rates of GyneFix IN IUD and MCu IUD were lower than that of TCu220C IUD(P<0.01).The removal rate of GyneFix IN IUD and MCu IUD were lower than that of TCu220C IUD(P<0.05).The 12 months cumulative continuation rates of GyneFix IN IUD and MCu IUD were higher than that of TCu220C IUD(P<0.01).Conclusions GyneFix IN IUD and MCu IUD can be recommended for clinical use based upon the advantages of lower pregnancy rate,lower removsl rate because of side-effect and higher cumulative continuation rate.Proper IUD should be selected depending on individuals,in order to increase the contraception effects and decrease the side-effects.