Objective The purpose of the study was to investigate the causes of hemoptysis and ex-plore the diagnostic procedures for hemoptysis in children. Methods The medical records of 42 children with hemoptysis admitted to the Respiratory Department of Hunan Children′s Hospital from June 2012 to June 2015 were collected by a retrospective study. The causes of hemoptysis were classified by pathogenesis,and the diagnosis of hemoptysis was evaluated in this study. The final diagnosis was divided into clear diagnosis and the cause was unknown. Results A total of 41(97. 6%) cases were diagnosed,1(2. 4%) case was not diagnosed. In 42 cases of hemoptysis,infection was the main cause of the disease,including 8(19. 0%) cases of pneumonia and 5(11. 9%) cases of bronchitis,and the following causes were bronchial artery fistula(10/42,23. 8%),idiopathic pulmonary hemosiderosis(8/42,19. 0%),bronchiectasis(5/42,11. 9%),pulmonary vascular malformation with pulmonary hypertension(3/42,7. 2%),pulmonary tuberculosis(2. 4%),bronchi-al foreign body(1/42,2. 4%) respectively. Thirteen cases of respiratory tract infection,and 5 cases of bron-chiectasis were diagnosed by chest CT,10 cases with pulmonary artery fistula were diagnosed by digital sub-traction angiography,8 cases of pulmonary hemosiderosis,1 case of pulmonary tuberculosis,and 1 case with bronchial foreign body were diagnosed by fiberoptic bronchoscopy. Three cases of pulmonary vascular malfor-mation and pulmonary hypertension were diagnosed by row helical CT angiography and cardiac color ultra-sound. Conclusion Hemoptysis in children is a heterogeneous group of respiratory disorders. Most causes could be identified by using the diagnostic procedures. Other factors, including medical history, CT, fiber bronchoscopy,multidetector CT angiography, cardiac color ultrasound and digital subtraction angiography, play important roles in the diagnosis of hemoptysis in children.

BACKGROUND: Medical order-obeying behavior(MOOB) in patients with essential hypertension(EH) cau directly affect the progression and prognosis of the disease. What factors, in turn, affect MOOB?OBJECTIVE:To analyze factors affecting MOOB in EH patients.DESIGN: Cross-sectional study.SETTING: Foreign Guests Ward of Qindu Stomatological Hospital, Fourth Military Medical University of Chinese PLA.PARTICIPANTS: A total of 205 patients with EH were admitted into the Foreign Guests Ward of Qindu Stomatological Hospital, Fourth Military Medical University of Chinese PLA from January 1998 to August 2000. Inclusion criteria: In accordance with WHO diagnostic criteria for hypertension. Exclusion criteria: Diabetes and other cardiovascular diseases. Altogether 164 patients with the mean age of (51 ± 5) years, 122 men and 42 women , accorded to the inclusion criteria.METHODS: The 164 patients were investigated with the self-designed questionnaire concerning MOOB in EH patients; patients' sex, educational level, family economic conditions, disease duration and other factors affecting MOOB were observed.MAIN OUTCOME MEASURES:Factors related to the patients' MOOB.RESULTS:53.0% (87/164) patients followed the medical orders and persisted in exercise therapy, 58.5 % ( 96 / 164 ) patients followed reasonable diet, 51.6% (32/164)patients quitted smoking and alcohol drinking, 82. 3% (135/164) received regular recheck-up, 64.6% (106/164) followed the medicinal treatment prescribed. Sex did not influence MOOB significantly. The rate of medical-order disobedience was higher in illiterate people and those with education below junior middle school level, but lower in those with education above senior middle school level(x2 =7.25, P < 0.01) . The disobedience rate was lower in patients with the mean income of > 500 yuan/month than in those with < 500 yuan/month(x2 = 39.4, P < 0.01),and was also lower in patients with disease duration < 1 year than those with disease duration > 1 year (x2 =5.66, P < 0.05).CONCLUSION; Medical order-obeying behavior in EH patients is poor, and it is significantly influenced by patients' educational level, family economic conditions and disease duration.

Mesenchymal stem cells(MSCs), which can be introduced in brain tissue by the strategies of brain parenchyma, blood and neurolymph transplantation, to repair tissue injury and promote functional recovery of neural system, have been considered as the ideal seed cells for the treatment of central nervous system diseases. However, as the special structure exists in the blood-brain barrier, study on correlation between MSCs and blood-brain barrier permeability will make it possible for more MSCs to pass the barrier to perform the treatment. This paper gives a review on the latest research development in the field.

Objective To analyse the etiology and clinical characteristics of 26 critically ill children with severe hand foot and mouth disease (HFMD) of Shanxi province in 2010.Methods We retrospectively analysed the clinical data of 26 children with severe HFMD from Mar to Sep 2010.Nucleic acid of enterovirus 71 ( EV71 ) and Coxsackie virus A 16 ( CoxA16) were detected in 20 out of 26 children with HFMD by reversed real time polymerase chain reaction (rRT-PCR),and the whole VP1 gene of EV71 deriving from 6 different areas of Shanxi province was amplified,sequenced,and compared with strains from other areas in china.Results EV71 nucleic acid were positive in 18 out of 20 children,while the other two were negative for EV71 and CoxAl6.Among all the critical cases,20 cases (76.9％) occurred in Weinan area,four in Xianyang area,and two in Xi'an urban area.Compared with those of Fuyang Anhui,Hong Kong China,Guangzhou,Shenzhen,Shandong,Beijing,the homology of the whole VP1 gene sequence from 6 strains of Shanxi area was 96％ ～ 100％.Most of the critical children were under 3-year-old,and the incidence rate of male children was higher than that of female children.All affected children had persisted fever,poor energy,hyperarousal,hypersomnia,and limb shaking.Meanwhile their peripheral blood leukocytes,C-reactive protein and blood glucose were markedly increased,but renal injuries were rare.Eighteen children clinically recovered on discharge,among which 2 cases had sequelae of limb activity obstacle,and 8 cases died.Conclusion Weinan is the area with the highest incidence rate of critical HFMD cases in Shanxi Province,and the major etiological organism is EV71,which is highly homologous to EV71 found in other regions of mainland China.As many cases are in dangerous condition,thus early identification and intervention could inhibit the disease progression,and play a key role in reducing the mortality.

AIM: To study the effect of high fat diet on the expression of sterol regulatory element biding protein-1 (SREBP-1) and transforming growth factor β_1 (TGF-β_1) in renal tubular cells and rosiglitazone intervention. METHODS: Wistar rats were treated with high fat diet and rosiglitazone for 3 months. The serum glucose, serum insulin and serum triglyceride were detected. Oil Red O staining was used to observe the renal lipid deposit and Masson staining was for the detection of ECM accumulation. SREBP-1, TGF-β_1 and FN protein were determined by the methods of immunohistochemistry and Western blotting. SREBP-1 mRNA was detected by in situ hybridization. RESULTS: Rosiglitazone prevented effectively the increase in serum glucose, serum insulin and serum triglyceride resulted from high fat diet. High fat diet led to lipid droplet formation in renal tubular cells and interstitial ECM accumulation, which was decreased by rosiglitazone treatment. Compared to normal rats, SREBP-1 protein and SREBP-1 mRNA showed high expressions in high fat diet rats that were lowered by rosiglitazone. The precursor segment and mature segment of SREBP-1 protein were decreased by 27.39% and 27.32%. Similarly, the high expressions of TGF-β_1 and FN protein in kidney of high fat diet rats were also prevented by rosiglitazone intervention. Compared to high fat diet rats, the expression of TGF-β_1 in rosiglitazone treatment rats was lowered by 19.14%. CONCLUSION: Rosiglitazone prevents effectively the over-expression of SREBP-1 and TGF-β_1 in renal tubular cells, and decreases lipid accumulation and ECM production in rats fed with high fat diet.

Objective To evaluate the diagnostic value of quantitative tissue velocity imaging ( QTVI ) in the detection of right ventricle function in a rabbit model of pulmonary artery embolism .Methods Twenty-two healthy adult rabbits were used in this study .The animal models of acute pulmonary embolism were created by injecting gelatin sponge into the ear vein of rabbits .The changes of right ventricle function were dynamically observed at immediately , 1 week, and 3 weeks after the establishment of acute pulmonary embolims models .QTVI images of the 4-chamber view at cardiac apex were recorded and analyzed by off-line analysis.Results The VP was significantly reduced immediately after the models were built compared with the data before modeling in both the basal and middle segments , the TQ-S was prolonged , and Ve/Va>1 (P<0.05).The VP at1 week after modeling was not significantly decreased than the data before modeling in both the basal and middle segments , and the TQ-S was prolonged, but the Ve/Va ratio was <1 (P<0.05).At 3 weeks after modeling, the VPwas decreased compared with the data before modeling , and the Ve/Va ratio was <1 (P<0.05). The intra-group comparison showed that the V P of the basal segment of right ventricle free wall was enhanced than that of the middle segment (P<0.05), butTQ-S was not obviously prolonged in both the two segments .Conclusions Quantitative tissue velocity imaging can reflect the contraction and relaxation of right ventricle in rabbits precisely .It is a good method to evaluate the function of right ventricle in animal models of pulmonary artery embolism.

graduate from self-study examination of nursing,the quality evaluation scale for graduation thesis of undergraduate from serf-study examination of nursing was developed.Methods The quality evaluation scales,which involving the scale using for faculty advisers,the scale using for reviewing teachers and the scale using for thesis defense teachers,for graduation thesis of undergraduate from self-study examination of nursing were constructed by adopting literature method,and so on.The scales were evaluated by reliability analysis,validity analysis,correlation analysis and factor analysis.Results The scale using for faculty advisers included 5 first-level indicators and 16 second-level indicators.The scale using for reviewingteachers included 4 first-level indicators and 14 second-level indicators.The scale using for thesis defense teachers included 5 first-level indicators and 14 second-level indicators.Their content validity indexes were respectively 0.909,0.952 and 0.864.Their construct validity cumulative contribution rates of the dimensions factors were respectively 72.77％,66.19％ and 63.79％.Their internal consistency reliability coefficients Cronbach α were respectively 0.953,0.810 and 0.926.The correlations between each factor and total score were significant.Conclusions three evaluation scales have good reliability and validity,which could be used to evaluate graduation thesis quality of undergraduate from self-study examination of nursing.

Objective To study the behavioral changes of the spinal cord injury model to provide important data for neuroal factor therapy of the impaired spinal cord.Methods In SD rat,aged 8 weeks,(T7~T8 spinous processes as control),after the spinous process and corresponding lamina of vertebra were removed,the spinal cord was cut by using scissors to prepare the complete transversal abruptly spinal model.The behavioral score of the rat was recorded at different time.The evaluation was made in imageology and pathology.Results The score of SD rat in the experiment group was higher than the control group at different control time.The pathobiology and imageology alterations were very apparent in the damaged spinal area.Conclusion GAP-43 has neuroprotective effect on the recovery of spinal cord injury.

Objective:To investigate the mainstream view and statusof people on body donation in Yunnan province, and provide references. Methods:Random sampling questionnaire survey was used, the obtained data was statistically analyzed. Results:Only 48. 0% of the people understand the body donation, 20. 6% of people know body donation ways and procedures, 27. 8% of people have heard of body receiving station, 11. 4% of people know the body receiving station in yunnan province, 17. 2% of the people to understand body donation of relevant laws and regulations. 47. 4% of people that are willing to donate his body. Conclusion:Make perfect laws and reg-ulations, respect for the body, giving donors and their families allowance, body donation monument, to carry out education and publicity of the body donation form to the whole society support and participate in the good atmos-phere of body donation has great benefits.

Objective To investigate the therapeutic effect of Losartan on ADM,ET-1 and their receptors in the kidney of diabetic rats.Methods Experimental diabetes was induced in uninephrectomized rats by STZ.The animals were divided into three groups: group A(control group),group B(diabetic group) and group C(Losartan-treated group).Immunohistochemistry,Western bloting,in situ hybridyzation and RT-PCR were used to detect the protein and mRNA expressions of ADM,ADMR,ET-1 and EDNR-A.Results Compared with group A,the expressions of ADM,ADMR,ET-1 and EDNR-A in group B significantly increased.After Losartan treatment,those indicators decreased.Conclusion The expressions of ADM,ET-1 and their receptors may be responsible for the renal protective effect of Losartan in diabetic rats.