Objective To investigate the value of laparoscopic operation in the diagnosis and treatment of closed abdominal injury.Methods Forty-seven cases of closed abdominal trauma were diagnosed and treated under laparoscope from December 2003 to July 2006.Under general anaesthesia or epidural anesthesia,an incision about 1 cm was made below the umbilicus.Then artificial pneumoperitoneum was established through an open tunnel or the veress needle(according to patient's condition).The camera was inserted through a trocar to clarify the diagnosis.If a confirmative diagnosis was not obtained,one or two additional trocars were inserted for further exploration. When the diagnosis was identified,another two or three trocars were inserted according to the location of lesions and corresponding treatment was performed based on pathogenetic condition.Results All the 47 cases were confirmatively diagnosed under laparoscope,including 15 cases of hepatorrhexis,13 cases of splenic rupture,9 cases of small intestinal rupture,3 cases of mesenteric injury,2 cases of pancreatic injury,2 cases of retroperitoneal hematoma,and 3 cases of intraabdominal multiple organ injury.Laparoscopic treatment was completed in 28 cases,a conversion to open surgery was needed in 17 cases,and conservative treatment was given in 2 cases of retroperitoneal hematoma.No surgical related death or complications happened.Conclusions Laparoscopy can be used in closed abdominal trauma to achieve a high diagnostic accuracy and to give a timely,effective,and proper treatment.

Objective To investigate the expression of hepatocyte growth factor receptor (HGFR) in epiretinal membranes (ERM) of eyes with proliferative vitreoretinopathy (PVR) and cultured retinal pigent epithelium (RPE) cells. Methods Fifteen human epiretinal membranes were obtained from eyes undergone vitrectomy for rhegmatogenous retinal detachment complicated with PVR and observed by immunohistochemical examination to study the expression of HGFR. Using the immunohistochemical technique to evaluate the expression of HGFR in cultured RPE cells. Results In 6 membranes of PVR grade C, HGFR were expressed in 5/6, and 7 cases were detected in 9 membranes of PVR grade D.RPE cells express readily detectable levels of HGFR. Conclusion The findings indicate that HGF might be involved in the formation of epiretinal membranes in PVR.

Objective To investigate the effect of HGF on proliferation and migration in cultured human RPE cells. Methods Human RPE cells cultured in serum-free medium were treated with HGF(1,2,10,50,100 ?g/L), and MTT assay was used to detect the growth of the cells; an in vitro wound healing model was used to count the number of cells that had entered the denudate area in RPE migration treated with HGF (1,2,10,50,100 ?g/L) after 20 h. Results HGF(10,50,100 ?g/L) increased proliferation rates of cultured human RPE (18 2 % to 34 8 %), and at a concentration of 50 ?g/L on day 3 HGF induced the maximal increase of proliferation( P

Sepsis caused by complicated intra-abdominal infection has a poor prognosis since the mechanism of sepsis has not been fully clarified .Research about the effect of Receptor of Advanced Glycation Endproducts on sepsis was paid extensive attention . In this paper , the construction and function of RAGE , the relationship between RAGE and inflammation and action of RAGE during sepsis are summarized .

ObjectiveTo investigate the clinical application value of early enteral nutrition after laparoscopic hepatectomy for hepatocellular carcinoma (HCC).MethodsA total of 49 patients with HCC undergoing laparoscopic hepatectomy in Sun Yat-sen Memorial Hospital of Sun Yat-sen University between November 2013 and June 2014 were included in this prospective study. These patients were divided into the enteral nutrition group and the parenteral nutrition group according to the random number table method. Twenty-five patients were divided into the enteral nutrition group, among them, 20 were males and 5 were females with the average age of (49±17) years old. Twenty-four patients were divided into the parenteral nutrition group, among them, 18 were males and 6 were females with the average age of (51±14) years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. The patients in the enteral nutrition group were given Ensure nutrient solution orally 24 h after operation for 7 d and the patients in the parenteral nutrition group were given parenteral nutrition 24 h after operation for 7 d. The total human albumin supplemented volume, recover time of gastrointestinal function, length of hospital stay after operation and hospitalization expenses of the two groups were compared. The nutritional support associated complications were observed. The data of the two groups were compared usingt test or Wilcoxon rank-sum test.ResultsThe total human albumin supplemented volume after operation of the enteral nutrition group was (40±11) g, which was signiifcantly lower than (45±10) g of the parenteral nutrition group(t=-16.089,P<0.05). The recovery time of gastrointestinal function after operation of the enteral nutrition group was (25±8) h, which was signiifcantly shorter than (43±11) h of the parenteral nutrition group (t=-36.928,P<0.05). The length of hospital stay after hepatectomy of the enteral nutrition group was (8±2) d, which was signiifcantly lower than (10±3) d of parenteral nutrition group (t=-12.025,P<0.05). The total hospitalization expenses of the enteral nutrition group were RMB 45,000 (37,000-61,000) yuan, which were signiifcantly lower than RMB 49,000 (42,000-67,000) yuan of the parenteral nutrition group (Z=-18.495,P<0.05). Five patients in the enteral nutrition group and 8 patients in the parenteral nutrition group developed abdominal distention and diarrhea, which were alleviated by adjusting doses or infusion speed. ConclusionsEarly enteral nutrition after laparoscopic hepatectomy for HCC is beneifcial to the postoperative recovery of gastrointestinal function and may effectively reduce the ifnancial burden of patients. Moreover, its overall curative effect is better than that of parenteral nutrition.