AIM To explain the variation of blood vessel factors on pulmonary hypertension(PH) induced by monocrotaline(MCT). METHODS Rats were given an injection of MCT ih(60 mg?kg -1 ). The concentration of endothe line(ET), nitric oxide(NO), thromboxane B 2(TXB 2) and 6 keto prostaglandin F 1? (6 keto PGF 1? ) were measured by radioimmunoassay and colorimetric analysis after the model had been set up successfully for 4 wk. RESULTS MCT either raised 6 keto PGF 1? and NO or prevented the progression of ET and TXB 2. CONCLUSION The mechanism of PH induced by MCT probably associated with the variation of those blood vessel factors.

Objective: To evaluate the malignant potential of oral lichen planus (OLP) by measuring and analyzing the dimensions of spinous cell in mucosal epithelium.Methods:Paraffin-embedded samples of oral mucosa, including normal (N,7 cases), OLP(16 cases), oral leukoplakia(12 cases) with mild and moderate epithelial dysplasia (LK) and well differentiated oral squamous cell carcinoma (SCC,10 cases), were analyzed by MIAS-99 System.The planar and stereoscopic dimensions of spinous cells were measured and compared.Results:The values of morphometric and stereologic parameters in OLP were between those in normal mucosa and in LK, significantly different from those in SCC and not significantly different from those in normal epithelium.Conclusion:Based on the changes of cell morphometric parameter value, OLP is generally a benign lesion with some degree of malignant potentiality that is less than LK.Nucleus/cytoplasm volume ratio and volume density of cell nucleus of spinous cells in mucosal epithelium are of value in distinguishing benign from maliganant lesions.

Objective To investigate the ultrasonic features and differential diagnosis of superficial neurilemmoma ,and improve diagnostic accuracy of superficial neurilemmoma by ultrasound .Methods Retrospectively analyse the ultrasonic findings of 51 ca-ses ,which confirmed for superficial neurilemmoma by pathology ,and analyse the misdiagnosis reason from the aspects of size , shape ,boundary ,internal echo ,blood flow signals .Results Superficial neurilemmoma had some characteristics in the shape ,bound-ary ,internal echo ,blood flow signals and so on .However ,the key point to differentiate it from other diseases was to identify the re-lationship with peripheral nerve .In this study ,the coincidence rate of ultrasonoic diagnosis with pathologic diagnosis was 78 .4% . Conclusion Superficial neurilemmoma have some similarity with neurofibroma or sebaceous cyst on ultrasonographic images .When making diagnosis ,especial attention should be paid to identify the relationship of neurilemmoma with peripheral nerve .

Objective:To observe the effect of photodynamic therapy(PDT)on apoptosis of human lung adenocarcinoma cell line A549.Methods:Human lung adenocarcinoma cell line A549was used and the optimal parameters of PDT were se-lected by MTT colorometric assay.The experiment was divided into2groups:control group and PDT group.Twenty-four hours after PDT(HPD concentration10mg/L,laser dosage10J/cm 2 ),cell cycle and apoptosis rate were estimated by flow cytometry;agarose gel electrophoresis and TUNEL were used to observe the DNA fragments and apoptosis index(AI).Re-sults:The killing effect was saturated when HPD was10mg/L and laser was10J/cm 2 .Under the same laser(10J/cm 2 ),A549cells were exposed to6different duty time fabrications and the outcomes showed that their D 492 had no statistical differ-ence.Agarose gel electrophoresis of DNA showed DNA ladder,a biochemical hallmark of apoptosis in the PDT group.Flow cytometry showed that PDT group had G 0 /G 1 stage arrest.The apoptosis rate was(18.443?7.122)%in PDT group vs (0.301?0.361)% in control group(P

Objective To explore the magnetic properties of permanent magnet wafers commonly used in magnetotherapy to provide a basis for the scientific and rational use of sources of magnetism.Methods A magnetometer was used to measure the magnetic induction intensities of the N and S polar centers of eight groups of magnet wafers (axial magnetization) with nominal magnetic induction intensities of 6 mT, 10 mT, 20 mT, 30 mT, 60 mT,100 mT, 200 mT and 350 mT.Results There were some differences between the nominal values and the measured mean magnetic induction intensities. In a few groups the differences were close to 20％. There were also differences within the same group. The magnetic induction intensities of the two polar surfaces were not always equal for the same magnet, and there were large differences among individual magnets.Conclusions The intensities of magnetic sources must be measured before use to ensure the consistency of magnetic induction intensities in therapeutic applications.

Objective: To study the preventive effect of lycopene (LP) on hyperglycemia in alloxan-induced diabetic rats, and reveal its possible mechanism.Method:Forty Sprague-Dawley rats were randomized into 5 groups:three groups of LP at doses of 5, 20, 50 mg(/kg bw ?d)for 15d, one control group and one model group of salad oil. Then alloxan (ALx) were injected intraperitoneally to the rats at dose of 150 mg/kg bw. At D4 and D14 after ALx injection, an oral glucose tolerance test (OGTT) was performed and body weight was measured, and at D14 GSP, FINS, NEFA, SOD,GSH-Px and MDA in liver were also determined . Results:Compared with the indices in group with ALX injection only,both the 2h blood glucose and GSP in group with medium-dose LP decreased significantly(P

Based on previous report that the Chinese herb Ligustrum lucidum (LL) extract directly inhibited hepatitis C virus (HCV) replicase (NS5B) activity, the active components of LL extract to inhibit HCV NS5B activity and their inhibition mode were investigated in this study. LL extract was separated using ethyl acetate and thin layer chromatography (TLC). The inhibitory activity of separated fractions on HCV NS5B was analyzed by the inhibitory assay of NS5B activity. The results showed that only fractions 1 and 2 inhibited NS5B activity, and fraction 2 possessed higher inhibitory activity than fraction 1. HPLC analysis combined with inhibitory assays indicated that ursolic acid and oleanolic acid are the active components within fractions 1 and 2 to inhibit NS5B activity, separately. Moreover, oleanolic acid possessed higher inhibitory activity than ursolic acid. Further inhibition mode analysis found that both oleanolic acid and ursolic acid suppressed NS5B activity as noncompetitive inhibitors. The Ki values of ursolic acid and oleanolic acid were about 4.7 microg x mL(-1) (10 micromol x kg(-1)) and 2.5 microg x mL(-1) (5.5 micromol x kg(-1)), respectively. Taken together, these results demonstrated that oleanolic acid and ursolic acid suppressed NS5B activity as noncompetitive inhibitors, implying that the two natural products have potential value for HCV therapy.

Multi-layer perceptron (MLP) neural network belongs to multi-layer feedforward neural network, and has the ability and characteristics of high intelligence. It can realize the complex nonlinear mapping by its own learning through the network. Bipolar disorder is a serious mental illness with high recurrence rate, high self-harm rate and high suicide rate. Most of the onset of the bipolar disorder starts with depressive episode, which can be easily misdiagnosed as unipolar depression and lead to a delayed treatment so as to influence the prognosis. The early identifica- tion of bipolar disorder is of great importance for patients with bipolar disorder. Due to the fact that the process of early identification of bipolar disorder is nonlinear, we in this paper discuss the MLP neural network application in early identification of bipolar disorder. This study covered 250 cases, including 143 cases with recurrent depression and 107 cases with bipolar disorder, and clinical features were statistically analyzed between the two groups. A total of 42 variables with significant differences were screened as the input variables of the neural network. Part of the samples were randomly selected as the learning sample, and the other as the test sample. By choosing different neu- ral network structures, all results of the identification of bipolar disorder were relatively good, which showed that MLP neural network could be used in the early identification of bipolar disorder.
Bipolar Disorder ; diagnosis ; Humans ; Neural Networks (Computer)

Objective To explore the value of somatosensory cortex evoked potential(CSEP)monitoring in objective evaluation of spinal cord function and integrity as well as prognostication of neural function after surgery.Methods CSEP monitoring was performed before the operation,during surgical procedure and after the operation in 56 spinal cord surgery patients to observe and analyze latency and amplitude of cortical potential.The significant changes were amplitude reduction≥150%or latency prolongation≥10%.Results There were 71.43%(40/56)true negative finding,both latency and amplitude without abnormal changes during the operation and without new neural functional deficit after operation.There was 0.18%(1/56)false negative finding,latency and amplitude without abnormal changes during surgical procedure,but with new neural functional deficit after operation.There were 26.79%(15/56)true positive findings,where latency transient prolongation<10%and amplitude reduction<50%during surgical procedure were observed.After suspending procedure or changing direction during surgical procedure,the waveform restored or improved.Conclusion CSEP monitoring during surgical procedure could reflect the sensitiveness to distracting,ischemia and injury of spinal cord.A timely intervention might improve the CSEP.CSEP could reflect the function and integrity of spinal cord.CSEP possess an important value in preventing spinal cord injury and evaluating the prognosis of neural function.

Objective To investigate the clinical value of high frequency ultrasound in discovering secondary pathological changes in infant with developmental dysplasia of the hip (DDH).Methods Thirtyfour infants with 36 dislocation hips were involved.Among them,32 were with unilateral dislocation of hip joint,2 were with bilateral dislocation of hip joint.High frequency ultrasound were used to observe the morphological changes of hip glenoid labrum,adipose hyperplasia in acetabulum,thickness variation of articular capsule,and development of the ossification center of the femoral head.Besides,the morphological changes of hip glenoid labrum and adipose hyperplasia in acetabul were compared with those from MRI.Results The sensitivity and specificity of ultrasound in diagnosing glenoid labral introversion were 82 ％ and 100％ respectively.The coincidence rate of diagnosing glenoid labral introversion and glenoid labral extroversion were both 83％.Adipose hyperplasias in acetabul were clearly seen in 31 hips using ultrasound.The thickness of articular capsule in the affected hip and in the healthy hip was (2.8 ± 0.6)mm versus (1.7 ± 0.4) mm,and the difference was obviously significant (P ＜0.01).The ossification center of the femoral head in the 34 infants were classified as the following four types:① which was smaller in the affected side than in the healthy side (22/34);②which was shown in the healthy side (7/34) but not in the affected side;③ which in both sides were not shown (4/34);④ which in both sides were small (1/34).Conclusions Ultrasound is a method of simple,rapid,non-invasive in diagnosing DDH,and it can be used to discover secondary pathological changes in infant with DDH,which provide important reference for clinical diagnosis and treatment.