106 patients with liver fibrosis, 58 men (54.72%) and 48 women (45.28%), were treated with Xinganbao. All of these cases were diagnosed as portal hypertension of advanced schistosomiasis. The drug was given in a daily dose of 2.25g (250mg/capsule) 3 times a day for a succession of 2 months.The result of our study suggests that Xinganbao therapy can relieve and/or eliminate the clinical symptoms of advanced schistosomiasis with liver function recovered, serum albumin raised, ?-globulin decreased, immune function adjusted and caliber of portal and spleen vein much diminished. The total rate of efficacy was 89.62%, of which, 14.15% (15 cases) markedly improved while 75.47% (80 cases) moderately.

Cone beam computer tomography (CBCT) has advantages of high precision, low radiation and high image quality. It has been developing quickly since it was applied clinically. In order to control X-ray TUBE HEAD effectively in Dental CBCT, X-ray TUBE HEAD Control System was designed and realized in this study. This control system is the core of CBTC system, which includes the communication between CBCT system and computer, the control of X-ray tube head by CBCT system main control board and the synchronization between main control board and the flat panel detector. Control circuit of the control system and corresponding operating software were designed with PIC16F877A as the core. This control system has been put into use in current CBCT system successfully.
Algorithms ; Cone-Beam Computed Tomography ; instrumentation ; Equipment Design ; Software

OBJECTlVE To establish Tg(-6.3CYP3A65∶EGFP) transgenic zebrafish for quick, intuitive detection of heavy metals ( copper, cadmium and zinc) , dioxin-like PCBs ( PCB126) and other environmental pollutants. METHODS Tol2 transposon system was used to generate transgenic zebrafish lines Tg(-6.3CYP3A65∶EGFP) in which CYP3A65 promoter regualated labeled fluorescence. The effect of heavy mentals ( copper, cadmium and zinc ) and PCB126 on the relative amounts of CYP3A65 gene expression was determined by observing the change in fluorescence intensity. RESULTS The relative gene expression of CYP3A65 was significantly increased after 96 h exposure to copper 0.1 and 0.2μmol·L-1 , cadmium 0.35 and 0.7μmol·L-1 , zinc 1.5 and 3μmol·L-1 , and PCB126 2-32μmol·L-1 , respectively ( P<0.01) , but decreased after 96 h exposure to copper 0. 9 μmol·L-1 , cadmium 2. 7 and 5.4 μmol·L-1 , and zinc 24μmol·L-1 , respectively( P<0.01) . CYP3A65 gene expression was significantly increased after 168 h exposure to copper 0.1 and 0.2 μmol·L-1 , cadmium 0.35 and 0.7 μmol·L-1 , zinc 1.5 and 3 μmol·L-1, and PCB126 2-32 μmol·L-1, respectively(P<0.01), but decreased after 168 h exposure to copper 0.9 μmol·L-1, cadmium 2.7 and 5.4 μmol·L-1, and zinc 12 and 24 μmol·L-1( P<0.05) , in a concentration-dependent manner. CONCLUSlON The results suggest that zebrafish CYP3A65 gene expression and the CYP3A65 labeled fluorescence lines can be another candidate biomarker for detecting environmental pollutants.

The present study aimed to explore the neuroprotective effect and possible mechanisms of rhGLP-1 (7–36) against transient ischemia/reperfusion injuries induced by middle cerebral artery occlusion (MCAO) in type 2 diabetic rats. First, diabetic rats were established by a combination of a high-fat diet and low-dose streptozotocin (STZ) (30 mg/kg, intraperitoneally). Second, they were subjected to MCAO for 2 h, then treated with rhGLP-1 (7–36) (10, 20, 40 µg/kg i.p.) at the same time of reperfusion. In the following 3 days, they were injected with rhGLP-1 (7–36) at the same dose and route for three times each day. After 72 h, hypoglycemic effects were assessed by blood glucose changes, and neuroprotective effects were evaluated by neurological deficits, infarct volume and histomorphology. Mechanisms were investigated by detecting the distribution and expression of the nuclear factor erythroid-derived factor 2 related factor 2 (Nrf2) in ischemic brain tissue, the levels of phospho-PI3 kinase (PI3K)/PI3K ratio and heme-oxygenase-1 (HO-l), as well as the activities of superoxide dismutase (SOD) and the contents of malondialdehyde (MDA). Our results showed that rhGLP-1 (7–36) significantly reduced blood glucose and infarction volume, alleviated neurological deficits, enhanced the density of surviving neurons and vascular proliferation. The nuclear positive cells ratio and expression of Nrf2, the levels of P-PI3K/PI3K ratio and HO-l increased, the activities of SOD increased and the contents of MDA decreased. The current results indicated the protective effect of rhGLP-1 (7–36) in diabetic rats following MCAO/R that may be concerned with reducing blood glucose, up-regulating expression of Nrf2/HO-1 and increasing the activities of SOD.
Animals ; Blood Glucose ; Brain ; Diet, High-Fat ; Hypoglycemic Agents ; Infarction ; Infarction, Middle Cerebral Artery ; Malondialdehyde ; Neurons ; Neuroprotective Agents ; Phosphotransferases ; Rats* ; Reperfusion ; Reperfusion Injury* ; Streptozocin ; Superoxide Dismutase

【Objective】 To evaluate the factors affecting urinary stone detection rate using virtual unenhanced (VUE) images obtained from triphasic dual-energy CT urography (DECTU) based on Logistic regression analysis. 【Methods】 For this study, 150 patients who had suspected urinary stone and underwent triphasic DECTU were included. The true unenhanced (TUE) images were reconstructed as 120 kVp-like images, and VUE images at the portal venous phase [VUE(VP)] and excretory phase [VUE(EP)] were obtained using iodine removal technique from portal venous and excretory phase DECTU images, respectively. Two readers independently evaluated the above three types of images, and recorded the number of urinary stones, their anatomical locations, and whether there was residual iodine on the VUE images. Stone size and CT number were recorded only on the TUE images. Stone size, CT number, anatomical location, and iodine contrast agent were included in univariate and multivariate Logistic regression analyses to evaluate the factors affecting urinary stone detection rate using VUE images. Thresholds for detecting urinary stones on VUE images were determined using receiver operating characteristics (ROC) analysis. 【Results】 We detected 304 stones on TUE images, while the detection rates were 92.4% and 71.4% when using VUE (VP) and VUE (EP) images, respectively. Stone size and CT number were important factors influencing urinary stone detection rate using VUE (VP) and VUE (EP) images (P<0.01). The area under curve (AUC) of using stone size and CT number for detecting stones using the VUE (VP) images was up to 0.96, and as threshold values, stones with size larger than 3.52 mm and CT number greater than 469 HU were found to have high accuracy. However, the AUC decreased to 0.88 when we combined stone size, CT number and anatomical location using the VUE (EP) images. In addition, different contrast agents did not affect the detection rate of stones on the VUE (EP) images (P=0.57). The stone detection rate in the kidney was significantly lower than those on the VUE (EP) images (P<0.001). 【Conclusion】 VUE (VP) images provide better stone detection. Stone size and CT number have significant impacts on the stone detection rate using VUE images. The lower stone detection rate in the kidney on the VUE (EP) images is related to the residual iodine.