Objective To explore the MSCT manifestations of chromophobe renal cell carcinoma (CRCC)and to improve its accu-racy of preoperative diagnose.Methods Clinical and MSCT finding were retrospectively reviewed in 14 patients with CRCC,which were confirmed by surgical pathology or biopsy.Results (1)On plain scanning,most of the CRCC lesions showed round or oval (86.7%,13/1 5)and isodensity (73.3%,1 1/1 5)mass;and the border of 66.7% (10/1 5)lesions were clear.53.3% (8/1 5)of the lesions were heterogeneous with lamellar cystic low density (8 lesions)and coarse ringed and/or foliated calcifications (4 lesions). (2)On contrast-enhanced sacanning,the CRCC lesions showed mild to moderate (86.7%,13/1 5)and heterogeneous (60%,9/1 5) enhancement,and 73.3% (1 1/1 5)of the lesions were persistent enhanced.(3)According to the pathology,80% (12/1 5)of the le-sions was the typical type,20% (3/1 5)was the eosinophilic type,and 0 was the hybrid type.Conclusion CRCC demonstrates cer-tain characteristics signs at MSCT examination.Lesion mostly shows well-circumscribed round or oval mass in the renal parenchy-ma,mild to moderate,heterogeneous and continuous enhancement on the contrast scanning.The diagnosis of CRCC should be con-sidered especially when the lesion has cystic change and coarse ringed and/or foliated calcifications.

Objective To observe the expression of heparanase(HPA)in kidney of diabetic nephropathy(DN)rats and to investigate the role of HPA in the pathogenesis of proteinuria in DN rals. Methods DM rat models induced by intraperitoneal injection with streptozotocin were constnmted.Twenty-six rats were randomly divided into heahhy control group(n=6),DM6-week group(n=10)and DM 12-week group(n=10).Relative kidney weight(RKW),blood glucose,BUN,Scr,24-hour urine volume and(t 24-hour proteinuria quantilation were measured,and renal morphology was observed after 6 and 12 weeks.The expression of HPA was examined by immunohistochemistry and reverse transcription PCR. Results (1)Conq)ared with the control group,RKW,blood glucose,BUN,24-hour urine volume and 24-hour proteinuria quantitation of DM groups increased markedly(P＜0.05 orP＜0.01).(2)Compared to the control group,the expression of HPA mRNA and protein in DM groups increused significantly(P＜0.01).(3)HPA protein and mRNA were positively correlated with the quantification of urinary prolein (r=0.783,P＜0.01;r=0.793,P＜0.01). Conclusion The increased expression of HPA maybe parlicipate in the pathogenesis of proteinuria in DN.

AIM　To investigate the antithrobosis effects of glycol mannate sulfate(GMS). METHODS　Vein thrombus was formed in rat by ligating postcava. Thrombus formation in vitro was observed by Chandlers method. Clotting indexes were measure by ACL200 congulation system. RESULTS　GMS dosage of 20、40 mg*kg-1 could inhibit the vein thrombus formation in rats (P<0.01). GMS could inhibit the thrombosis in rabbit, in vivo, and the inhibitory action was enhanced along with the increase of dosage. The CT,TT,CT,APTT,RT and PT were prolonged, and the fibrinogen and the activity of Ⅱ and Ⅱa were decreased. The activity of ATⅢ was enhenced. CONCLUSION　GMS has antithrombotic effect and the mechanism may be related to the anticongulat effect.

AIM:To investigate the role of peripheral blood leukocytes and polymorphonuclear neutrophils (PMNs) on intestinal injury following mesenteric ischemia/reperfusion (IR) in rats. METHODS: Twenty adult, male Sprague-Dawley rats, weighing 200-230 g, were randomly divided into two groups. The control group (CON) consisting of 10 rats was subjected to laparotomy and separation of superior mesenteric artery (SMA) only. The ischemia/reperfusion (IR) group consisting of 10 rats, was subjected to laparotomy, followed by occlusion of the superior mesenteric artery (SMA) by an atraumatic microvascular clamp for 30 min. At the end of ischemic period in IR, the microvascular clamp was removed and the intestinal segment was reperfused for 60 min. The pathological changes of the ileal mucosal tissue were evaluated. The apoptosis of intestinal mucosal epithelial cells was examined by terminal deoxylnucleotidy-l transferase mediated-dUTP nick end-labeling (TUNEL). The enzymatic activity of casapse-3 in mucosal cells was determined using a colorimetric assay. The percentages of apoptotic peripheral blood leukocytes and PMNs were measured by flow cytometry using Annexin-V/PI double staining assay. The numbers of peripheral blood leukocytes in each animal was measured at baseline, 30 min of ischemia, and 30 min and 60 min of reperfusion. RESULTS: (1) Compared to CON group animals, the most severe mucosal injury was observed in IR group under optical microscope. (2) The number of apoptotic mucosal epithelia cells and enzymatic activity of caspase-3 were significantly higher in IR than those in CON group (P

Objective To investigate the correlations of serum levels of NOD-like receptor family CARD domain containing 3(NLRC3)and extracellular matrix protein 1(ECM1)with the develop-ment of acute respiratory distress syndrome(ARDS)in patients with sepsis.Methods A total of 133 patients with sepsis were enrolled in sepsis group,and 80 healthy individuals during the same pe-riod were included in control group.The sepsis group was further divided into ARDS group(52 cases)and non-ARDS group(81 cases)based on the presence or absence of ARDS.Serum levels of NLRC3 and ECM1 expression were measured using enzyme-linked immunosorbent assays(ELISA).Multivari-ate Logistic regression analysis was used to identify factors associated with the development of ARDS in sepsis patients.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the predictive value of serum NLRC3 and ECM1 levels for ARDS in sepsis patients.Results Compared with the control group,the sepsis group had significantly lower serum NLRC3 level and higher ECM1 level(P＜0.05).The incidence of ARDS in the 133 sepsis patients was 39.10％(52/133).Com-pared with the non-ARDS group,the ARDS group had significantly lower serum NLRC3 level and higher ECM1 level(P＜0.05).Independent risk factors for the development of ARDS in sepsis pa-tients were increased Sequential Organ Failure Assessment(SOFA)score,elevated blood lactate level and increased ECM1 level,while increased NLRC3 level was an independent protective factor(P＜0.05).The area under the curve for the combined prediction of serum NLRC3 and ECM1 expres-sion in sepsis patients with ARDS was 0.887,which was greater than 0.811 and 0.792 predicted by serum NLRC3 and ECM1 expression alone(P＜0.05).Conclusion Decreased serum NLRC3 lev-el and increased ECM1 level are closely associated with the development of ARDS in sepsis patients.The combined assessment of serum NLRC3 and ECM1 level has a high predictive value for ARDS in sepsis patients.

Objective To investigate the correlations of serum levels of NOD-like receptor family CARD domain containing 3(NLRC3)and extracellular matrix protein 1(ECM1)with the develop-ment of acute respiratory distress syndrome(ARDS)in patients with sepsis.Methods A total of 133 patients with sepsis were enrolled in sepsis group,and 80 healthy individuals during the same pe-riod were included in control group.The sepsis group was further divided into ARDS group(52 cases)and non-ARDS group(81 cases)based on the presence or absence of ARDS.Serum levels of NLRC3 and ECM1 expression were measured using enzyme-linked immunosorbent assays(ELISA).Multivari-ate Logistic regression analysis was used to identify factors associated with the development of ARDS in sepsis patients.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the predictive value of serum NLRC3 and ECM1 levels for ARDS in sepsis patients.Results Compared with the control group,the sepsis group had significantly lower serum NLRC3 level and higher ECM1 level(P＜0.05).The incidence of ARDS in the 133 sepsis patients was 39.10％(52/133).Com-pared with the non-ARDS group,the ARDS group had significantly lower serum NLRC3 level and higher ECM1 level(P＜0.05).Independent risk factors for the development of ARDS in sepsis pa-tients were increased Sequential Organ Failure Assessment(SOFA)score,elevated blood lactate level and increased ECM1 level,while increased NLRC3 level was an independent protective factor(P＜0.05).The area under the curve for the combined prediction of serum NLRC3 and ECM1 expres-sion in sepsis patients with ARDS was 0.887,which was greater than 0.811 and 0.792 predicted by serum NLRC3 and ECM1 expression alone(P＜0.05).Conclusion Decreased serum NLRC3 lev-el and increased ECM1 level are closely associated with the development of ARDS in sepsis patients.The combined assessment of serum NLRC3 and ECM1 level has a high predictive value for ARDS in sepsis patients.