Objective To discuss the effect of Jun laevis gauze on wound healing after hemorrhoids surgery. Methods 80 patients after hemorrhoids surgery were randomly divided into the control group and the observation group with 90 patients in each group. The observation group was applied with the Jun laevis gauze in the anal canal and anus everyday, the control group received Kau cream dressing change. The clinical effect was compared between the two groups. Results The observation group was significantly better than the control group in reducing postoperative wound pain, hemorrhage and edema, the average length of hospital stay and wound healing time was significantly shorter than the control group. Conclusions Jun toad gauze can alleviate hemorrhoid pain, bleeding, edema, symptoms, and promote wound healing, shorten hospital stays and reduce patient suffering.

Minimally invasive ablation is one of the most effective therapy methods for liver tumor. Eastern Hepatobiliary Surgery Hospital, Second Military Medical University is responsible for the training of refresher physicians in liver tumor minimally invasive ablation. However, due to the different professional background of the refresher physician, the conventional teaching method is not enough. Through the train-ing of clinical thinking, image reading ability, ultrasound guide operation and the management of intraop-erative and postoperative complications in many aspects targeted teaching, reaching the purpose of training, increasing the experience of popularities of minimally invasive ablation technology, and providing a reference basis for future refresher physician teaching in liver tumor minimally invasive ablation.

Vitamin D has a wide range of biochemical effects. Insufficiency/ deficiency of vitamin D increases the risk of insulin resistance by ways of inflammation,adipocytokines,oxidative stress and mitochondria function,it could lead to metabolic disorder of glucose and lipid,which closely relates to the incidence and development of obesity and metabolic syndrome on children. This paper presents the relationship between vitamin D with obesity and metabolic syndrome on children,states the possible mechanisms of regulation of glucose/ lipid metabolism and insulin resistance by vitamin D.

Objective:To establish an HPLC method for the simultaneous determination of olmesartan medoxomile, amlodipine be-sylate and hydrochlorothiazide in compound olmesartan medoxomile tablets. Methods:The desired chromatographic separation was a-chieved on a Phenomenex C18 (250 mm × 4. 6 mm, 5μm) column. With gradient elution, the optimized mobile phase consisted of ace-tonitrile as solvent A, and 50 mmol·L-1 potassium dihydrogen phosphate buffer (pH 3. 0, containing 0. 05% triethylamine) as sol-vent B. The flow rate was set at 1. 0 ml·min-1 , the column temperature was 30 ℃, the UV detection was carried out at 236 nm and the sample size was 20μl. Results:The compounds were separated well, the linearity between the peak area and the concentration was observed within the range of 4. 0-80. 0 mg·L-1(r=0. 999 9)for olmesartan medoxomile, 1. 0-20. 0 mg·L-1(r=0. 999 9)for amlo-dipine besylate and 1. 25-25. 0 mg·L-1(r=0. 999 9)for hydrochlorothiazide. The average recovery of olmesartan medoxomile, amlo-dipine besylate and hydrochlorothiazide was 99. 1%(RSD=1. 31%, n=9), 100. 3%(RSD=1. 21%, n=9) and 100. 2%(RSD=1. 06%, n=9), respectively. Conclusion:The method is specific and stable in the determination of olmesartan medoxomile, amlo-dipine besylate and hydrochlorothiazide in the tablets.

Objective To evaluate the role of computer-assisted surgical planning system in liver resection for patients with hepatocellular carcinoma (HCC).Methods Between January 2010 and January 2012,21 patients with HCC who underwent liver resection were included in this study.Computer assisted operative planning was performed before surgery.Results Computer-assisted surgical planning was performed successfully in all the 21 patients.The three dimensional anatomic evaluation was precise and quantitative.The predicted liver resection volume showed a significant correlation with the actual volume,with a mean difference of 3.5％.The types of liver resection included anatomic liver resection (15 patients) and local resection (6 patients).There was a 0％ mortality and a 14.3％ morbidity (3/21).Conclusions The computer-assisted surgical planning system reliably analyzed the 3D vascular structures and predicted accurately liver resection volumes.It can help to promote precise and safe liver resection.

Intrabeam,a newly developed intraoperative radiotherapy in recent years,has many advantages such as accurate positioning,small size,easy to move and requiring a lower of protection.It is widely used in the treatment of breast cancer,colorectal cancer,lung cancer,bone metastases tumors and other malignancies.Intrabeam as a new way of intraoperative radiotherapy,is expected to provide more options for the treatment of a variety of neoplasms.

Objective To explore the clinical significance of MDR1 gene expression inpatients with gastric cardia carcinoma.Methods Using reverse transcription-polymerase chain reaction(RT PCR), the genes expression of MDR1 and MRP, in 4 6 cases of gastric cardia cancer was determined.Results Positive expression of MDR1 and MRP gene were 6:3% and 50% respectively, which was muchhigher than that in tissue adjacent to 1ilo cancer site. In 9 cases of pre-operative chemotherapy , the levels ofMDR 1, and MRP gene expression were higher higher that without chemotherapy (P

Objective:To establish an HPLC method for the simultaneous determination of telmisartan and amlodipine besylate in tablets. Methods:The isocratic separation was achieved on a Phenomenex C18 column (150 mm × 4. 6 mm, 5 μm) with the mobile phase composed of 50 mmol·L-1 sodium dihydrogen phosphate buffer (pH 6. 0, 0. 5% triethylamine)-acetonitrile (40:60, v/v). The flow rate was 1. 0 ml·min-1 , the detection wavelength was 254 nm, the column temperature was 30℃ and the sample size was 20μl. Results:Telmisartan could be well separated from amlodipine besylate under the conditions mentioned above. The linearity be-tween the peak area and the concentration was obtained within the range of 4. 0-80. 0 μg·ml-1(r=0. 999 9) for telmisartan and 1. 0-20. 0 μg·ml-1(r=0. 999 9) for amlodipine besylate. The mean recovery of telmisartan and amlodipine besylate was 99. 90% and 100. 52%, and RSD was 0. 74% and 1. 48%, respectively (n=9). Conclusion:The method is specific, stable and accurate in the determination of compound telmisartan tablets.

The yeast one hybrid system is the new type system deriving from the yeast two hybrid system to study the interaction between DNA and protein.The principle and the technical line of this system was briefly introduced,and the progress of this system on the research of plant transcription factor gene resistant to osmotic stress was particularly summarized,such as cloning the transcription factor genes of osmosis resistance type;confirming the interaction between known DNA and protein;making sure the localization of the confirmed DNA binding domain with the interaction;also validating the transcription activation of the transcription factor gene.Furthermore,the problems existing in this system on the research of plant transcription factor gene resistant to osmotic stress was analyzed,and the development prospect according to the research was forecasted.

Purpose:To determine the efficacy and toxicity of gemcitabine plus cisplatin in anthracyclin- and taxane-pretreated patients with advanced breast cancer. Methods:Chemo-resistance was defined as no response while rece iving first-line and/or second-line chemotherapy. Patients with advanced and c hemo-resistant breast cancer, which was confirmed histologically, received gemc itabine 1,000 mg/m 2 on d1, 8 and cisplatin 25 mg/m 2 on d1-3, every 3 weeks.2 cycles were delivered at least. Results:30 female patients were enrolled in this trial. All pat ients were evaluable for response and toxicity. The median age was 46 years (ran ge, 34-58 years). After 2-4 (median, 3) cycles of chemotherapy, overall object ive response was 50.0%, with 5 CRs (Complete response 16.7%) and 10 PRs (Partial response 33.3%). Stable disease was seen in 13 patients (43.3%), and disease pr ogression in 2 patiens (6.7%). Toxicities included myelosuppression and gastroin testinal reaction mainly. Grade Ⅲ-Ⅳ toxicites developed in 50% patients. Ther e were no treat-related deaths. The median follow-up time was 12.5 (range: 2- 48) months. Median overall survival was 14 months, and median time to progressio n was 10 months. Conclusions:These data indicate that the combination of gemcita bine and cisplatin is an effective treatment for heavily pretreated breast cance r patients with manageable toxicity.