Objective To study the distribution of HBV genotypes and the relationship between HBV genotypes and the clinical characteristics in Hangzhou area. Methods The genotype, content of HBV-DNA, serological indicators and AFP were detected by RT-PCR, time resolved immunofluorescence method respectively in 146 cases of HBV-DNA positive patients. The relationship between HBV genotype and the clinical characteristics were analyzed by the statistical analysis.Results In the HBV genotyping, there were 75 cases of type B (51.4%),62 cases of type C (42.5%),9 cases of no B or no C type (6.2%);In type C, the HBV-DNA, HbsAg and HbeAg content were significantly higher than the type B.The content of AFP in type C was higher than type B, but with no statistical difference(P>0.05). Conclusion In Hangzhou area, the priority genotype of HBV is type B. Genotype has a certain relationship with the clinical character-istics and the severity of the disease.

Abstract: A high-performance liquid chromatographic (HPLC) method for the assay of pregabalin gastric retention sustained-release tablets was established, successfully solving the problem of low recovery of pregabalin through a special sample pretreatment method. By comparing salting-out and excipient dispersion, the pretreatment methods to overcome the viscosity of the test solution were established. Both methods can be used for the determination of the product content, but the salting-out method is easier to operate. The HPLC conditions were Inertsil ODS-3 (4.6 mm×0.25 m, 5 µm) column with mobile phase of 3.4 g/L potassium dihydrogen phosphate (pH adjusted to 6.3 by ammonia) and methanol (85︰15);the column temperature was 30 ℃;the flow rate was 1.0 mL/min;the sample size was 50 µL;and the detection wavelength was 210 nm. Through the validation of the salting-out method, the average recovery of the drug was 99.74% and the RSD was 0.43%; the precision test RSD was 0.77%;the test solution was stable within 12 h;the chromatographic system had good durability;and the excipient did not interfere with the content detection.The method is stable, reliable and suitable for the assay of pregabalin gastric retention sustained release tablets.

Objective: To study the application value of improved early warning score in the practice of theory and practice interactive infiltration training mode for neurosurgery interns. Methods: A total of 84 neurosurgery interns were selected as study samples and randomly divided into control group and intervention group by random double blind method, each group had 42 interns. The control group adopted the interactive infiltration model of theory and practice. The Intervention group adopted the interactive infiltration training mode of theory and practice under the improved early warning scoring system design. Statistics and comparison of the performance of the two groups of trainee nurses, the evaluation of teaching teachers and patients, the self-evaluation of trainee nurses, and the occurrence of nursing errors during the internship. Results: The related examination results between the two groups showed significance (t value was 7.182,5.462,5.530, P<0.01). The evaluation for nursing students by teachers and patients also showed significant difference (t value was 6.989-8.790, P<0.01). The incidence of error event in the intervention group was 9.52% (4/42), lower than that in the control group [30.95% (13/42)]. There was statistical difference between two groups (χ² value was 14.227, P<0.01). Conclusions The application of the improved early warning score in the theory and practice interactive infiltration training mode of neurosurgery interns can improve the satisfaction of teachers and patients to interns, enhance the self-satisfaction of interns and reduce the incidence of nursing errors of interns.