Objective:To study the effect of survivin expression on the mature process of dendritic cells from mouse bone marrow. Methods:The cells in marrow as the precurosor of the dendritic cells were taken from mouse in bioclean condition. Then the iDC were induced by the precurosor of DCs with GM-CSF and IL-4 in medium. ThemDC were induced by the precurosor of DCs with GM-CSF,IL-4,and TNF-? in medium. Survivin was compared by RT-PCR,and Western-bolt among these three stages. Results:The RT-PCR showed that the expression ratios of survivin mRNA among the precurosor of DCs,iDC,and mDC were(0.735?0.081),(0.596?0.073,P

Objective To detect the serum level of interleukin (IL)-1 1, lymphocyte subsets and NK cells in patients with idiopathic thrombocytopenic purpura (ITP), and explore the related factors in the pathogenesis of ITP. Methods The serum level of IL-11, lymphocyte subsets and NK cells were detected by double antibody sandwich enzyme linked immunosorbent assay (ELISA) and flow cytometry in 50 ITP patients (ITP group) and 30 controls (control group). Results The platelet in ITP group [ (30.21 ± 19.40) ×109/L] was lower than that in control group [ (207.21 ± 31.55 ) × 109/L] obviously (P ＜ 0.05 ); the serum level of IL-11 in ITP group [(255.72 ± 163.43) ng/L] was significantly higher than that in control group [ (40.60 ± 5.57 ) ng/L ] (P ＜ 0.05 ). The correlation analysis indicated that the blood serum levels of IL- 11 had negative relationship with the platelet (r = -0.557 ,P ＜ 0.05). The percentage of CD3+ and CD4+ T lymphocyte percentage, CD4+/CD8+ in ITP group were lower and the percentage of CD8+ T lymphocyte was higher than those in control group obviously (P ＜ 0.05 ). The percentage of CD3- CD(16+56) +NK cell in ITP group was lower than that in control group (P ＜ 0.05). Conclusion IL-11, lymphocyte subgroup and NK cell change correlate with ITP morbidity closely, and the IL-11 level and the platelet possibly have the negative feedback control action.

Objective To analyze the clinical feature and diagnostic methods of patients with ischemic colitis(IC) for improving the recognition and the efficacy of therapeutic treatment. Methods Retrospective analysis of the clinical data of 30 cases of IC,including the medical history,clinical symptomatology,endoscopic appearance, histopa-thology,treatment and prognosis. Results IC occurred most of frequently in mid-aged and elders,the male-female ra-tio was 1:2.3,83.3% of the patients were over 60 years old, and most them had underlying disease. The most com-mon presenting features were sudden abdominal pain and bloody stools. Endoscopic lesions located mainly in left co-lon. Mucosal edema,erosin and submucosal bleeding were common pathological feature. Vasodilator drugs and compre-hensive medical therapy had good effects. The average duration for healing or improving was 10 days. Conclusions IC should be suspected in all elder patients with sudden abdominal pain and bloody stools. Colonoscopy is helpful for diagnosis. The prognosis is good in most cases and with rapid recovery.

This paper describes the relationship between standards and intellectual property rights,describes the importance of putting the intellectual property protection into the International standards of Chinese medicine by the examples.According to the Chinese medicine's own characteristics and actual conditions in our country,the author points out some suggestions on how to put the intellectual property protection into the International standards of Chinese medicine.

In this paper,we analyze the current situation of the national standardization on acupuncture needles and related patents.Through analyzing the international research focus on the acupuncture needle patents,we give some strategic suggestions on the intellectual property protection of acupuncture needles and traditional Chinese medicine.

objective: To study the effects of laser engineered net shaping(LENS) in the manufacture of dental prosthesis. Methods: An experiment LENS system, including a 5 kW continuous wave CO 2 laser, a four-axes numerical control working table and powder feeding system, was built up, by which, a rectangular LENS component was made from Rene95 alloy powder. The microstructure of the component was observed with scanning electron microscope and ESA analysis. Results: The size of the component was 120 mm?15 mm?15 mm , and the wall of the component was perpendicular to the base. The microstructure of the component was made up of fine columnar dendrite with the length of 10～20 ?m, 10 times less than that of the cast made from the same material. The main elements of the powder distributed homogeneously,the negative effect of segregation could be limited and the properties of the component could be improved.Conclusion: The microstructure of components made by LENS is much better than that of those made by casting from the same material.

0.05). The effect of group A was better than that of group B in relieving symptoms and improving routine blood and urine examination indexes, MG and urinary creatinine levels, blood lipid content and renal function (P

ColV~+ strains of Escherichia coli produced larger inhibitory zones when these strains grown on nutrient agar containing phosphate after overlaid sensitive indicators. This appears that production of colicin V is increased by the addition of phosphate to nutrient agar. It was sure that stimulation of phosphate to colicin V formation results from its effect in reducing divalent cation levels in nutrient agar since adding EDTA to nutrient agar had the same effect as phosphate, but the addition of Mg~(2+) or Ca~(2+) had the oppsite effect. Therefore nutrient agar supplemented with phosphate can be used to isolate and identificate ColV~+ strains of E. coli.

Objective To investigate the clinical diagnostic value of transperineal ultrasound in women in late pregnancy with stress urinary inconti?nence. Method Totally 66 cases of women in late pregnancy were selected,who did not suffer from SUI(groupⅠ),and 54 cases of women in late pregnancy who suffered from SUI(groupⅡ). Another 63 cases of healthy childless women were used as normal control(groupⅢ). Transperineal ultrasound was used to dynamically observe their pelvic floor structure,and the ultrasonic parameters were recorded and analyzed,including the posi?tion of bladder neck in X axis and Y axis((Djx,Dzx,Djy,Dzy)during resting period and tension period,the vesicourethral angle(αj,αz),the Bladder angle(βj,βz). The moving of the bladder neck in X axis(ΔDx=Dzx-Djx)and Y axis(ΔDy=Dzy-Djy),as well as the bladder rotation an?gle(Δβ=βz-βj)were calculated and the movement of the bladder neck(ΔD)was measured,followed by comparative analysis. The threshold val?ues of all ultrasonic parameters were determined by the ROC curve analysis. Results The bladder neck of all three groups were backward during tension period. Compared with groupⅢ,ΔDy,Djx,Dzx obviously decreased in groupⅠand groupⅡ,whileβj,βz,αj increased significantly.ΔDx of groupⅡwas significantly larger than that of groupⅢ(P<0.05);There was significant difference in Djy,Dzy,ΔD,Δβandαz among the three groups(P<0.05);In ROC curve analysis,when the critical value of Djy,Dzy,ΔDy,ΔD,αz andΔβwas-1.75 cm,-1.71 cm,0.25 cm,0.29 cm,131.5° and 3.5° ,their sensitivity was 88.9%,88.9%,72.2%,83.3%,88.9%and 72.2%,and the specificity was 72.2%,81.8%,72.7%, 95.5%,86.3%and 68.2%,respectively. Conclusions The transperineal ultrasound could clearly show the pelvic floor of the women in late preg?nancy,the bladder urethral support structure damage or defect was more serious in the women in late pregnancy who were suffering from SUI,and the determination of the critical values of all ultrasonic parameters helped the screening of SUI in women in late pregnancy.

Coverage of internal iliac artery during endovascular abdominal aortic aneurysm repair may cause ischemic complications in buttock,intestine,lower extremity and genital.We reported nursing care of 72 patients in our center to investigate the key points of nursing.Our experience were as follows:individualized blood pressure management and doctor-nurse cooperation in pain management were key points in preoperative nursing;close postoperative observation was focused on ischemic colitis,buttock claudication and ischemic buttock skin;cooperation with ostomy care group improved pertinency of nursing care;rounding by integration of doctors and nurses obtained high satisfaction and improved patients' compliance.