Objective:To evaluate the effect of special teaching on anti-bacterial drugs that applied the problem-based learning combined with case-based learning.Method:s A total of 100 pharmaceutical students who participated in internships in Qujing First People's Hospital of Yunnan Province from 2018 to 2019 were included. According to the career plan after graduation, the students were divided into experimental group (to be engaged in clinical pharmacy) and control group (to be engaged in drug dispensing). There were 50 people in the experimental group, who received the problem-based learning combined with case-based learning teaching mode on the basis of traditional theoretical teaching (basic theoretical knowledge teaching). There were 50 people in the control group, who received only the traditional teaching mode. After the internship, the quality of teaching was assessed through examinations and questionnaires.Result:s There were no significant differences in the overall scores and individual scores of the objective questions ( P>0.05). The overall scores and individual scores of the case analysis of the experimental group were significantly higher than those of the control group ( P<0.05). The enthusiasm and initiative, and ability of clinical thinking, innovative practice, communication, team work of the experimental group were significantly better than those of the control group ( P<0.01). Conclusion:The problem-based learning combined with case-based learning teaching method is superior to the traditional theoretical teaching mode, which can significantly improve the teaching quality of antimicrobials for pharmacy interns. It is worth being promoted in teaching.