According to topographic anatomy, pathogenesis and by retrieving, summarizing and analyzing literature regarding needle-knife and needles with knife-edge for carotid cardiac syndrome, it is found out that clinical misdiagnosis rate of carotid cardiac syndrome is considerably high. Needle-knife and needles with knife-edge could significantly improve the clinical symptoms of carotid cardiac syndrome, showing characteristic and advantage in treatment, but it is deficient in technique standard and efficacy criteria that should be united and authoritative. Researches regarding pathogenesis of carotid cardiac syndrome are not systematic. Clinical observation regarding long-term efficacy and relapse of needle-knife and needles with knife-edge treatment is rare. It is believed that the awareness on carotid cardiac syndrome should be increased to reduce misdiagnosis; scientific and standardized technique standard and efficacy criteria should be established; systematic and comprehensive researches regarding mechanism of needle-knife and needles with knife-edge for carotid cardiac syndrome should be launched; besides, clinical discussion regarding its long-term efficacy should start to provide a better clinical guideline.
Acupuncture Therapy ; instrumentation ; methods ; Heart Diseases ; therapy ; Humans ; Needles ; Treatment Outcome

Objective To study the effectrve approach of the nutrition support in burn patients. Methods The means of immuno-precipitation-deduction,ELISA and fluore-photometion were used to test the change of activities,protein expression of the 19S regulator and the rate of protein degradation in skeletal muscle in scard rats with enteral feeding or parenteral nutrition. Results Compared with parenteral nutrition , enteral feeding could markedly reduce the activity and protein expression of the 19S regulator ,and the digeneration of skeletal muscle was also lower. Conclusions The early enteral feeding can distinctly inhibit the system of 26S proteasome , thereby reduce the protein degradation of skeletal muscle in scald rats,which may be benefical to the metabolic modulation of the burned patients.

Objective:To establish a method for the content uniformity ( CU) of fennel oil in Weitongning tablets to study the CU of fennel oil in Weitongning tablets. Methods: Methyl salicylate as the internal reference, the content of trans-anethole in fennel oil was determined by GC. The separation was performed on a DB-FFAP capillary column (30 m × 0. 32 mm, 0. 25 μm) at 145℃, the injection part temperature was 180℃ and the FID detector temperature was 230℃, nitrogen with high purity was used as the carrier gas at the flow rate of 0. 8 ml·min-1 . The air flow rate was 400 ml·min-1 and that of hydrogen was 35 ml·min-1 . The methodology validation was carried out, and the samples from different companies with different batches were determined. Results:Significant differ-ence showed in the CU of fennel oil in the samples with different batches from the same manufacturer or from different companies. The trans-anethole showed linear correlation within the range of 2. 189-35. 030 μg · ml-1 ( r =0. 999 9 ) and the average recovery was 100. 06% (RSD=0. 5%, n=9). Conclusion:The method is simple and quick. It is accurate in the CU determination of fennel oil and can be used for the quality control of Weitongning tablets.

Objective To explore the mechanism of negative nitrogen balance in the treatment after burns. Methods The means of immuno precipitation deduction and ELISA were used to test the activities and contents of 26S proteasome and 19S regulator in skeletal muscle of rats inflicted with 30%TBSAⅢ burns. Results TNF? markedly raised the activities and contents of 26S proteasome and 19S regulator in skeletal muscle after scalding. Conclusion TNF? activates the 26S proteasome system in skeletal muscle, thus it enhances the degradation of protein, which is associated with the development of negative nitrogen balance following scalding.

Objective To investigate the efficacy and safety of domestic Oseltamivir granules in the treatment of influenza A in children.Methods The research subjects were the hospitalized patients with influenza A coming from the Department of Infectious Disease in Guangzhou Women and Children's Medical Center.from January and March,2014 They were divided into 2 groups randomly.Patients in the treatment group received domestic Oseltamivir granules,while the patients in the control group received foreign Oseltamivir.Chi square test was performed to compare baseline characteristics and the difference in side effects.Paired t test was used to compare baseline characteristics and the difference in side effects.Results There were 44 cases in the study group,27 cases were male(61.36％),17 cases were female (38.64％),and the average age was (2.40 ± 1.97) years old.There were 40 cases in the control group,28 cases were male (70.00％),12 cases were female (30.00％),and the average age was (2.99-± 3.19) years old.The maximum age was 6 years old.The age and gender were not significant (P ＞ 0.05) between 2 groups.The fever remission time was (14.67-± 9.37) h and (11.80-± 9.98) h in the treatment group and the control group,and there was no significant difference between 2 groups (P ＞ 0.05);the flu symptoms completely remission time was (62.50 ± 25.03) h and (65.40 ± 36.74) h in the treatment group and the control group,and there was no significant difference between 2 groups (P ＞ 0.05);the adverse reaction was not observed in the treatment group,but 1 case in the control group had cough,and without special treatment cough alleviated gradually;the close contacts in 2 groups did not develop influenza A.Conclusions The domestic Oseltamivir granules are as effective and safe as the foreign Oseltamivir.And the domestic Oseltamivir granules are the granular type,and therefore more suitable for children

Objective To identify the features of the NCAM+ c-Kit+ subset of hepatic progenitor cells in the intrahepatic cholangiocarcinoma (ICC) cell line RBE.Method Magnetic activated cell sorting was used to isolate NCAM+ c-Kit+/NCAM-c-Kit-subset cells,which were tested for hepatic progenitor cell properties and proliferation,colony formation,and invasive abilities in nude mice.Resuits The cell proliferation ability of NCAM+c-Kit+ subset cells was stronger than that of NCAMc-Kit-subset cells (P＜0.01).In serum-free condition,the number of colonies formed by NCAM+c-Kit+ subset cells was more than that of NCAM-c-Kit-cells (P＜0.01).1 × 104 NCAM+c-Kit+ cells were enough to form tumors in nude mice after subcutaneous inoculation for two weeks,while 1 × 106 NCAM-c-Kit-cells were necessary to form tumors for three weeks.The tumor formation rate of NCAM+c-Kit+ cells was higher than that of NCAM-c-Kit-cells (P=0.04).Conclusions It is possible that NCAM+c-Kit+ subset cells in RBE have the properties of hepatic progenitor cells,and NCAM combined with c-Kit might be a valuable marker for isolating and purifying ICC stem/progenitor cells.

This study was aimed to find the fragmentation pathways of Schisandrin B using the Discovery Studio by electrospray ionization mass spectrometry (ESI-MSn). The first and multi-stage mass spectrum diagrams were obtained. The results showed that mass spectrometry fragments of Schisandrin B was analyzed under the positive mode, the cracking rings are mainly occurred, and free radicals such as H2O, -CH3 and -OCH3 were lost. It was concluded that this study enriched the mass spectral decomposition, and provided basis for the study on chemical constituents of lignan compounds.

Objective To investigate the circadian and seasonal distribution of cardiovascular events (CVE) and the relationship between average monthly temperature and the incidence of CVE. Methods A total of 5837 emergency patients with CVE were enrolled from 1997 to 2007. The exact admission time of each patient was registered. The average monthly temperature data from a regional weather station for this time period was supplied by the meteorological office of the city. The relationship between the average monthly temperature and the incidence of CVE was explored and the corresponding curves were plotted. Results The occurrence of CVE has obvious seasonal variation, and its a higher tendency of episodes was found in spring and winter periods. High incidence of acute myocardial infarction, angina pectoris, acute left-sided heart failure, hypertensive crisis, and sudden death occurred in spring and winter as wee as the time for a change of season, that is, January, February, March, April, October, November, and December. There was a significant negative correlation between the incidence and the average monthly temperature (P<0.005-0.001). Circadian rhythm of CVE was present, with a peak in the early morning and forenoon. Conclusion There are circadian and circannian rhythms of the incidence of CVE. Cold weather condition is a risk factor for the occurrence of CVE, which usually peaks in the early morning and forenoon.

Objective: To investigate the clinical characteristics of pertussis-associated pneumonia and analyze it's risk factors. Methods: Clinical data were taken from Shenzhen Children's Hospital with Bordetella pertussis infection and confirmed by culture or real-time polymerase chain reaction (PCR) of nasopharyngeal secretion from October 2013 to December 2015. Patients were divided into two groups, those with radiologically confirmed pneumonia in the course of their disease and those with not. Clinical data were retrospectively analyzed and compared. T test, Rank sum test or chi square test were used for comparison between groups. Risk factors were analyzed by unconditional Logistic regression analysis. Results: A total of 501 children hospitalized with Bordetella pertussis infection were included. Among them, 309 patients were diagnosed with pneumonia. The median age was 3 (2, 6) months. Symptoms were paroxysmal cough (n=252, 81.6%), tachypnea (n=69, 22.3%), and cyanosis (n=105, 34.0%). The time from onset of cough to radiologically confirmed pneumonia was between 1 and 66 days with a median of 9 (5.5, 15.0) days. The most common pathogen of coinfection was respiratory syncytial virus (RSV)(20 cases). Macrolides were used in 306 cases for (8.2±3.6) days. All cases showed significant improvement. There were more male children (62.1% (192/309) vs. 50.3% (95/189) , χ²=6.768, P=0.009), and more instances of comorbidities (13.3% (41/309) vs.5.8% (11/189) , χ²=6.957, P=0.008) in the pneumonia group than in the other. The age was younger (3 (2,6) vs.4 (2,6) months, Z=32.91, P=0.000) in pneumonia group than in the other. Male sex, younger age, and underlying disease were independent risk factors for pertussis-associated pneumonia (OR=1.648, 1.486, 2.695, P=0.008, 0.036, 0.005). Conclusions Pneumonia, as a complication of pertussis, is very easy to see in hospitalized children. The duration of hospitalization is extensive. It is more likely to happen in children who are male, young, and having underlying diseases. Pneumonia is easy to occur in the first 2 weeks of the course of disease.

Objective To investigate the value of serum interleukin-6 (IL-6) ,haptoglobin (HPT) and amy-loid A (SAA) in patients with acute exacerbation of chronic obstructive pulmonary disease (COPD) with pneumonia.Methods 87 cases of acute exacerbation of COPD without pneumonia and 76 cases with pneumo-nia ,admitted in the hospital from December 2015 to March 2017 ,were selected as COPD without pneumonia group and COPD with pneumonia group.62 healthy people who underwent the healthy assessment during the same period were selected as the control group.The changes of serum IL-6 ,HPT and SAA levels in three groups ,the value of serum IL-6 ,HPT and SAA levels in detecting COPD with pneumonia and the correlation of IL-6 ,HPT and SAA were compared.Results COPD patients with serum IL-6 ,HPT and SAA were higher than group COPD with pneumonia group and control group ,and COPD is not associated with serum IL-6 , HPT and SAA in pneumonia group than the control group (P＜0.05) ;IL-6+ HPT+SAA diagnostic specifici-ty ,sensitivity and positive predictive value is higher than IL-6 ,HPT and SAA (P＜0.05) ;IL-6 and HPT was positively related with SAA and HPT was positively correlated with SAA.The serum levels of IL-6 ,HPT and SAA in the COPD with pneumonia group were higher than those in the COPD without pneumonia group and the control group ,and the serum IL-6 ,HPT and SAA levels in the COPD without pneumonia group were higher than those in the control group ,and the difference was statistically significant (P＜0.05).The diagnos-tic specificity ,sensitivity and positive predictive value of the combined detection of IL-6 ,HPT and SAA levels were higher than those of single detection of IL-6 ,HPT and SAA level ,and the difference was statistically sig-nificant (P＜0.05) ;IL-6 level was positively correlated with HPT and SAA levels ,and HPT was positively correlated with SAA level.Conclusion The levels of serum IL-6 ,HPT and SAA in patients with acute exacer- bation of COPD increased.The combined detection of the three had relatively high sensitivity and specificity , and the levels of IL-6 ,HPT and SAA were positively correlated.