AIM: To explore protective and therapeutic effects of the shenfu injection (SF) against renal ischemia reperfusionand and its possible mechanisms by studying the impact of SF on P38 mitogen activated protein kinase (P38MAPK), tumor necrosis factor alpha (TNF-?) and intercellular adhensive molecule-1 (ICAM-1). METHODS: 36 male SD rats were randomly divided into control group, ischemia reperfusion (IR) group and SF group. Rats were subjected to left renal pedicle occlusion followed by reperfusion with contralateral nephrectomy. The expression of P38MAPK and ICAM-1 were evaluated by Imunohistochemistry. The expression of TNF-? in the kidney tissue and plasma were analyzed by enzyme-linked immunoadsordent assay (ELISA). RESULTS: The expression of P38MAPK and ICAM-1, concentration of TNF-? in IR group were higher than that in the control group (P

Mammalian gonadotropin-releasing hormone(termed GnRHⅠ) is a decapeptide that plays key role in the process of reproduction.In addition to its well-known endocrine function, it has become evident that GnRHⅠ and the second GnRH subtype(termed GnRHⅡ) are potentially important autocrine and/or paracrine regulators in placental trophoblast cells.The effects of GnRHⅠ and GnRHⅡ on trophoblastic cell invasion were investigated in human choriocarcinoma cell line(JEG-3) by the methods of invasion assay, RNA interference, Western blot and Real-time PCR.The data revealed that exogenous native peptide of these two forms of GnRH significantly induced the expression of typeⅠ GnRH receptor(GnRHRⅠ) in JEG-3 cells.The specific siRNA for GnRHR Ⅰ was capable of blocking the invasion-promoting effect of GnRHⅠ, but did not influence the effect of GnRHⅡ.The data suggested that the two hormones may be elicited by distinct receptors.Interference of the activities of ERK and JNK was capable of attenuating GnRH Ⅰ and Ⅱ-induced MMP-2 expression and trophoblast invasion, indicating that activation of ERK and JNK are required in both GnRH Ⅰ and GnRH Ⅱ-mediated MMP-2 production and invasion-promoting effect in human trophoblastic cells.

Objective To investigate the production of reactive oxygen species and anti-oxidative protein of neutrophils in patients with coronary heart disease, and to evaluate the role of neutrophil activation in the pathogenesis of coronary heart disease. Methods Reactive oxygen species production was measured by chemiluminescence method, the degranulation of neutrophils was detected by the whole cell patch-clamp capacitance technique, the plasma and intracellular levels of myeloperoxidase(MPO) were quantified by photometry.Results The production of superoxide anion in neutrophils was increased in patients with stable or unstable angina more significantly than control group (344.21?64.4,325.65?65.1 vs 271.53?56.2,P

0.05). S.sobrinus strains had significantly lower initial adhesive ability to saliva coated hydroxyapatite in the absence of sucrose than S.mutans (P

砄bjective:To investigate the consistency of Streptococcus mutans and Streptococcus sobrinus isolates identified by colony morphology,biochemical tests and DNA base content analysis.Methods:46 clinical isolates (30 of S.mutans and 16 of S.sobrinus ) were identified by colony morphology,biochemical tests and determination of DNA base contents with reversed phase high performance liquid chromatography. S.mutans Ingbritt and S.sobrinus 6715 were used as the controls. Results:The DNA contents (G+C mol%) of S.mutans and S.sobrinus clinical isolates, identified by colony morphdogy and biochemical test, were 37.52 and 45.19 respectively,consistent with the expected 36～38 and 44～46 mol percent of G+C.Conclusion: S.mutans and S.sobrinus isolates,identified by colony morphology and biochemical tests ,were consistent with the DNA G+C content analysis. S.mutans and S.sobrinus can be easily and reliably identified by colony morphology and biochemical tests.

0.05).In spinal cord tissues of injury groups,the level of HIF-1? mRNA began to elevate at 3h after injury and reached the maximize on 3d,then it began to decline and returned to the basal level on 14d;the HIF-1? expression showed a time-dependent difference as followed: It began to increase at 3h after injury,peaked on 1d,then gradually decreased,and recovered to the normal level on 14d after injury.Conclusion The result suggested that hypoxia circumstance can enhance the stability of HIF-1? and the transcription of HIF-1? mRNA,and the time-dependent expression of HIF-1? and HIF-1? mRNA can be used to help the diagnosis of SCI and estimation of injury time.

Objectives To investigate the changes and signiifcance of serum tumor necrosis factor-α(TNF-α) and anti-ds-DNA antibody in systemic lupus erythematosus (SLE) and lupus nephritis (LN) in children. Methods Sixty-seven children with SLE among whom 52 children had LN were selected and 30 healthy children were selected as normal controls. The level of serum TNF-αwas measured by enzyme-linked immunosorbent assay, and the anti-ds-DNA antibody level was measured by radioimmunoassay. Results The serum levels of TNF-αand anti-ds-DNA antibody were signiifcantly higher in children with SLE or LN than those in healthy controls (P all<0.01). Furthermore, the serum levels of TNF-αand anti-ds-DNA antibody were signiifcantly higher in active phase than in stationary phase of SLE and LN (P all<0.01). The serum TNF-αlevel was positively correlated with SLE disease activity index score and serum anti-ds-DNA level in 67 SLE patients (P<0.05). Conclu-sions The serum levels of TNF-αand anti-ds-DNA antibody were increased in children with SLE and LN and were positively correlated with disease activity.

Objectives　To evaluate the reliability and validity of SF-36 in old people in community. Methods　 378 old people in community were investigated by SF-36 by face-to-face interview, and the score of items was analyzed.Reliability and validity of SF-36 were evaluated by item-internal consistency,item discriminant vadility,Cronbach's alpha coefficient, et al. Results　The clustering and ordering of the item means of SF-36 were the same as that hypothesized, except for items PF2,PF10,RP3,GH4. The correlation between an item and its hypothesized scale was 0.4 or above for all items. The Cronbach's alpha coefficient were above the standard of 0.7 for all except SF scale. Conclusions　SF-36 Health Survey Questionnaire is available for the elderly people. The present evaluation proposes some minor word and item changes which is more suitable for old people.

Objective:To investigate the effects of Qingxin Yangshen Chinese herbs on ?-adrenoceptor in cardiocytes of stress rats.Methods:Adopting the method of radoligand binding,the affinity of ?-adrenoceptor of experimental myocardium cells and density were analyzed.Meanwhile,RT-PCR was used to test mRNA of ?-adrenoceptor of myocardium cells.To observe the effect of Qingxin Yangshen Fang on ?-adrenoceptor of myocardium cells of stress rats,and the Zhusha Anshen Pills and Propranololas the control groups.Results:All the three groups above can increase the affinity of ?-adrenoceptor of experimental myocardium cells,promote its sensitivity and notably inhibit the decrease of density of receptors and control down-regulation of expression of ?1-ARmRNA induced by stress,and regulate up the expression of ?2-ARmRNA.Conclusion:The Chinese herbs of Qingxin Yangshen had the effect of acceptor inhibiting and can regulate the changes of ?-adrenoceptor of myocardium cells induced by stress.

ObjectiveTo explore the points of nursing cooperation during hand-assisted thoracoscopic surgery for simultaneous operation of bilateral lung metastasectomy.Methods The preoperative preparation,nursing cooperation during the surgery,the cleaning and maintenance of surgical instruments and equipment after operation were retrospectively summarized in 72 patients who underwent hand-assisted thoracoscopic surgery for simultaneous treatment of bilateral pulmonary metastases. ResultsAll the procedures of surgery were smooth.No patients were converted to thoracotomy or died of surgery. Conclusions It is an important challenge for nursing quality in the operation room and the cleaning and maintenance of surgical instruments and equipment in carrying out hand-assisted thoracoscopic surgery.The skilled nursing cooperation and standardized operating procedures could improve the quality of intraoperative nursing cooperation.It could ensure the smooth process of simultaneous operation of bilateral lung metastasectomy.