Mammalian gonadotropin-releasing hormone(termed GnRHⅠ) is a decapeptide that plays key role in the process of reproduction.In addition to its well-known endocrine function, it has become evident that GnRHⅠ and the second GnRH subtype(termed GnRHⅡ) are potentially important autocrine and/or paracrine regulators in placental trophoblast cells.The effects of GnRHⅠ and GnRHⅡ on trophoblastic cell invasion were investigated in human choriocarcinoma cell line(JEG-3) by the methods of invasion assay, RNA interference, Western blot and Real-time PCR.The data revealed that exogenous native peptide of these two forms of GnRH significantly induced the expression of typeⅠ GnRH receptor(GnRHRⅠ) in JEG-3 cells.The specific siRNA for GnRHR Ⅰ was capable of blocking the invasion-promoting effect of GnRHⅠ, but did not influence the effect of GnRHⅡ.The data suggested that the two hormones may be elicited by distinct receptors.Interference of the activities of ERK and JNK was capable of attenuating GnRH Ⅰ and Ⅱ-induced MMP-2 expression and trophoblast invasion, indicating that activation of ERK and JNK are required in both GnRH Ⅰ and GnRH Ⅱ-mediated MMP-2 production and invasion-promoting effect in human trophoblastic cells.

Objective To investigate the production of reactive oxygen species and anti-oxidative protein of neutrophils in patients with coronary heart disease, and to evaluate the role of neutrophil activation in the pathogenesis of coronary heart disease. Methods Reactive oxygen species production was measured by chemiluminescence method, the degranulation of neutrophils was detected by the whole cell patch-clamp capacitance technique, the plasma and intracellular levels of myeloperoxidase(MPO) were quantified by photometry.Results The production of superoxide anion in neutrophils was increased in patients with stable or unstable angina more significantly than control group (344.21?64.4,325.65?65.1 vs 271.53?56.2,P

AIM: To explore protective and therapeutic effects of the shenfu injection (SF) against renal ischemia reperfusionand and its possible mechanisms by studying the impact of SF on P38 mitogen activated protein kinase (P38MAPK), tumor necrosis factor alpha (TNF-?) and intercellular adhensive molecule-1 (ICAM-1). METHODS: 36 male SD rats were randomly divided into control group, ischemia reperfusion (IR) group and SF group. Rats were subjected to left renal pedicle occlusion followed by reperfusion with contralateral nephrectomy. The expression of P38MAPK and ICAM-1 were evaluated by Imunohistochemistry. The expression of TNF-? in the kidney tissue and plasma were analyzed by enzyme-linked immunoadsordent assay (ELISA). RESULTS: The expression of P38MAPK and ICAM-1, concentration of TNF-? in IR group were higher than that in the control group (P

Objectives　To evaluate the reliability and validity of SF-36 in old people in community. Methods　 378 old people in community were investigated by SF-36 by face-to-face interview, and the score of items was analyzed.Reliability and validity of SF-36 were evaluated by item-internal consistency,item discriminant vadility,Cronbach's alpha coefficient, et al. Results　The clustering and ordering of the item means of SF-36 were the same as that hypothesized, except for items PF2,PF10,RP3,GH4. The correlation between an item and its hypothesized scale was 0.4 or above for all items. The Cronbach's alpha coefficient were above the standard of 0.7 for all except SF scale. Conclusions　SF-36 Health Survey Questionnaire is available for the elderly people. The present evaluation proposes some minor word and item changes which is more suitable for old people.

砄bjective:To investigate the consistency of Streptococcus mutans and Streptococcus sobrinus isolates identified by colony morphology,biochemical tests and DNA base content analysis.Methods:46 clinical isolates (30 of S.mutans and 16 of S.sobrinus ) were identified by colony morphology,biochemical tests and determination of DNA base contents with reversed phase high performance liquid chromatography. S.mutans Ingbritt and S.sobrinus 6715 were used as the controls. Results:The DNA contents (G+C mol%) of S.mutans and S.sobrinus clinical isolates, identified by colony morphdogy and biochemical test, were 37.52 and 45.19 respectively,consistent with the expected 36～38 and 44～46 mol percent of G+C.Conclusion: S.mutans and S.sobrinus isolates,identified by colony morphology and biochemical tests ,were consistent with the DNA G+C content analysis. S.mutans and S.sobrinus can be easily and reliably identified by colony morphology and biochemical tests.

0.05). S.sobrinus strains had significantly lower initial adhesive ability to saliva coated hydroxyapatite in the absence of sucrose than S.mutans (P

0.05).In spinal cord tissues of injury groups,the level of HIF-1? mRNA began to elevate at 3h after injury and reached the maximize on 3d,then it began to decline and returned to the basal level on 14d;the HIF-1? expression showed a time-dependent difference as followed: It began to increase at 3h after injury,peaked on 1d,then gradually decreased,and recovered to the normal level on 14d after injury.Conclusion The result suggested that hypoxia circumstance can enhance the stability of HIF-1? and the transcription of HIF-1? mRNA,and the time-dependent expression of HIF-1? and HIF-1? mRNA can be used to help the diagnosis of SCI and estimation of injury time.

Objectives To investigate the changes and signiifcance of serum tumor necrosis factor-α(TNF-α) and anti-ds-DNA antibody in systemic lupus erythematosus (SLE) and lupus nephritis (LN) in children. Methods Sixty-seven children with SLE among whom 52 children had LN were selected and 30 healthy children were selected as normal controls. The level of serum TNF-αwas measured by enzyme-linked immunosorbent assay, and the anti-ds-DNA antibody level was measured by radioimmunoassay. Results The serum levels of TNF-αand anti-ds-DNA antibody were signiifcantly higher in children with SLE or LN than those in healthy controls (P all<0.01). Furthermore, the serum levels of TNF-αand anti-ds-DNA antibody were signiifcantly higher in active phase than in stationary phase of SLE and LN (P all<0.01). The serum TNF-αlevel was positively correlated with SLE disease activity index score and serum anti-ds-DNA level in 67 SLE patients (P<0.05). Conclu-sions The serum levels of TNF-αand anti-ds-DNA antibody were increased in children with SLE and LN and were positively correlated with disease activity.

Objective To investigate the application effect of flexible management in nursing of general surgery department. Methods 21 nursing staff and 836 patients from January to October, 2007 were selected as research object, and were given flexible management. 21 nursing staff and 797 patients from January to December were selected as the control. The influence of flexible management on nurses and patients and satisfaction degree with nursing work before and after application of flexible management were compared. Results Working and learning enthusiasm greatly improved,educational background and professional titles of nurses increased after application of flexible management. The average hospitalization time was 28 days,the per capita cost was 12 131 yuan,satisfaction degree with nursing was 87.0% before application of flexible management, but the average hospital stay of patients decreased to 20 days, the per capita cost decreased to 8764 yuan and satisfaction degree with nursing increased to 97.0% after application of flexible management. Conclusions Application of flexible management in nursing care of general surgery department showed significant effect,it is suitable for more application in practice.

A method using dry ashing combined with triple-phase ion-exchange chromatography was developed to enrich chlorine in the plant, which could eliminate the effect of organic impurities on the determination of chlorine isotope by thermal ionization mass spectrometry. In the procedure, the recoveries indicated that there was no loss of chlorine and no fractionation of chlorine isotopes occur. The results showed that the composition of chlorine isotope in the tissues of plants in Qinghai-Tibet Plateau area and Shandong area ranged from-1 . 79‰ to ﹢4 . 77‰ with an average of 1 . 20‰. δ37Cl values of the two plant samples in Shandong area not more than 0‰indicated that 35 Cl was enriched in the organs of the two plants andδ37Cl values in Qinghai-Tibet Plateau area more than 0‰ indicated the deficiency of 35 Cl. Chlorine isotope composition fractionated significantly in the plant samples or in different tissues of a plant. This may be caused by the differences of the medium where the plants grow, the transport of chlorine or the physiological effect in the uptake of chlorine by plants , which put forward a new insight for the further investigation of chlorine behavior in plant and the global cycling of chlorine in the biogeochemistry.