Objective To evaluate the clinic effect of two kinds of interventional intracavitary closure combined with deligation of varicose veins of lower extremity. Methods Seventy-five sick legs of 68 cases,23 sick legs of 21 cases accepted interventional intracavitary closure combined with high deligation, while 52 sick legs of 47 cases accepted interventional catheter electric coagulation combined with high deligation. Results The average hospitalization time was(2.7±1.4)days, lyins in bed time was 6 hours,12 sick legs(16.0%)suffered superficial skin mild numbness. The follow-up time was(7.3±3.0)months, all the varicose veins were occluded by color Doppler and no local recurrence was found. Conclusion Interventional intracavitary closure is a kind of safe, convenient and effective therapy to varicose veins of lower extremity.

Objective To evaluate the effect of inverse ratio ventilation (I ∶ E =1 ∶ 1) on ventilatory function during pneumoperitoneum in the obese patients undergoing laparoscopic surgery.Methods Sixty patients,aged 30-64 yr,of ASA physical status Ⅰ or Ⅱ],with body mass index of 30-40 kg/m2,scheduled for elective laparoscopic radical resection of rectal carcinoma,were divided into 2 groups (n =30 each) using a random number table:I∶E =1∶1 mode of ventilation after pneumoperitoneum (group A),and I∶E =1∶2 mode of ventilation after pneumoperitoneum (group B).Anesthesia was induced with midazolam,fentanyl,propofol and vecuronium.The patients were endotracheally intubated and mechanically ventilated.Anesthesia was maintained with infusion of propofol and remifentanil,sevoflurane inhalation,and intermittent iv boluses of vecuronium.In group A,the patients received I ∶ E =1 ∶ 1 after pneumoperitoneum.In group B,the patients received I ∶ E =1 ∶ 2.PaO2,PaCO2,peak inspiratory pressure (PIP),mean airway pressure (Pmean),dynamic compliance (Cdyn),intrapulmonary shunt (Qs/Qt),PETCO2,RR,MAP and HR were recorded immediately after intubation,and at 30,60 and 120 min of pneumoperitoneum.Results Compared with group B,PaO2,Pmean and Cdyn were significantly increased,and PIP and Qs/Qt were decreased,and no significant changes were found in RR,PET CO2,PaCO2 and hemodynamic parameters in group A.Conclusion Inverse ratio ventilation (I ∶ E =1 ∶ 1) can effectively improve ventilatory function during pneumoperitoneum in the obese patients undergoing laparoscopic surgery without exerting adverse effects on hemodynamics.

Objective To analyze the main causes of early neonatal death in Qingdao Women &Children's Hospital in the latest 10 years.Methods The medical records of early neonatal death in the Qingdao Women&Children’s Hospital from January 2002 through December 201 1 were analyzed.Results A total of 16 314 neonates were available to study,and 525 of them died.The mortality of early neonatal death within a week after birth was 77.71% (408 cases),including 214 premature infants and 194 mature infants.A comparison of mortality between the first 5-year and the second 5-year showed that the underlying trend of mortality was downward (P <0.05 ).The mortalities of early neonatal death within 1 day,3 days, and 4-7 days after birth were 1 10 cases (27.00%),192 cases (47.06%),and 106 cases (25.98%), respectively,and most of the neonates died within 3 days after birth;and the number of severe neonates died within 24 hours was higher than that in other two groups (P <0.01).The leading causes of early neonatal death in 10 years were:preterm birth-related factors (97 cases,23.78%),severe asphyxia (91 cases, 22.3%),congenital malformations (66 cases,16.2%).From 2002 to 2006,the top three main causes of early newborn death were severe asphyxia,congenital malformations and acute respiratory distress syndrome (ARDS),whereas from 2007 to 2011,the leading causes of death were ARDS,severe asphyxia,and congenital malformations.In comparison of early neonatal death between different gestational ages,preterm infants’death occurred mainly in 32 weeks,accounting for 26.72%.Conclusions In our hospital,the early neonatal mortality was decreased in the latest 10 years,and majority of deaths occurred within 3 days after berth,and preterm infants’death occurred mainly in 32 weeks.The leading causes of overall early neonatal death in order of frequency were premature birth,severe asphyxia and congenital malformations,but in later 5 years this order of leading causes changed.

ObjectiveTo explore relations between self-control and aggressive behavior in college students.Methods 310 students were surveyed with Buss-Perry aggression questionnaire and self-control questionnaire(SCQ).Results①High score group in SCQ was higher with physical aggression,verbal aggression,anger,hostility and aggression scores ( respectively ( 25.86 ± 5.04 ),( 15.27 ± 2.76 ),( 20.12 ± 4.26 ),( 23.87 ±4.45 ),( 85.11 ± 11.75 ) ) than the low group ( respectively (20.26 ± 5.04 ),( 14.04 ± 3.35 ),( 13.76 ± 4.68 ),( 19.37 ±5.31 ),(67.43 ± 13.56) ),the difference was statistically significant (P＜ 0.01 ).②Self-control scores had significant positive correlations with aggressive behavior scores( r=0.60,P＜ 0.01 ).③Self-control dimension (self-emotional,simplistic tendency,impulsive risk-taking) were significant predictors of aggressive behavior(P ＜0.01 ).ConclusionSelf-control is closely related with aggressive behavior,and it is a significant predictor of aggressive behavior.

AIM To investigate the anti-atherosclerosis mechanisms of arecoline. METHODS Fifty-eight male rats were assigned to control, model, arecoline 1 mg?kg -1 and arecoline 5 mg? [FQ(12。46,X-WZ]-kg -1 groups randomly. The rats in model and arecoline-supplied groups were fed with hypercholesterol diet. The following variables were measured: HE staining of rat aorta; serum level of TC, LDL-CHO, HDL-CHO, IL-8, ET-1 and NO; expression of eNOS protein on rat aorta; expression of MCP-1, ICAM-1, CXCR-2 and eNOS mRNA on rat aorta. RESULTS Arecoline increased plasma level of NO and the expression of eNOS protein and mRNA, decreased plasma level of IL-8 and the expression of ICAM-1, MCP-1 and CXCR-2 mRNA on rat aorta. CONCLUSION The anti-atherogenic effects of arecoline seems to be closely involved in increasing plasma level of NO and eNOS protein and mRNA expression, decrease in plasma IL-8 level and down-regulation of the expression of ICAM-1, MCP-1 and CXCR-2 genes.-

AIMTo investigate the anti-atherosclerosis mechanisms of the novel compound PPVP. METHODSThirty-six male rats were a ssigned to control, model and PPVP 5 mg?kg -1 groups randomly. The rats in model and PPVP 5 mg?kg -1 groups were fed with hypercholesterol diet for inducing atherolcerosis model. The following variables were measured: HE stainin g of rat aorta; serum level of TC, LDL-CHO, HDL-CHO, IL-8, ET-1, PGI 2, TXA 2 and NO. RESULTSPPVP increased serum level of NO, decreased plasma level of TXA 2 and inhibited the over expression of IL-8. CONC LUSIONThe anti-atherogenic effect of PPVP seems to be closely involved in increasing serum NO level, decreasing plasma level of TXA 2, and inhibiting over expression of IL-8. The result also suggests that the anti-atherogenic ef fect of PPVP in high cholesterol-fed rat may not due to the regulation of plasm a lipid profile, plasma level of ET-1 and PGI 2.

Objective To investigate the expression of phosphorylated mammalian target of rapamycin (p-mTOR) and phosphorylated eukaryotic translation initiation factor 4E binding protein 1 (p-4EBP1) in pathologic scar,and comprehend its role and significance in the pathologic scar formation.Methods SP immunohistochemical method was used to detect the expression of p-mTOR and p-4EBP1 in 20 cases of keloid,20 cases of hypertrophic scar,20 cases of non-pathologic scar and 20 cases of normal skin tissue.The positive rates of expression in different tissues were analyzed,and the relationship in pathological scar was explored.Results The positive rates of p-mTOR and p-4EBP1 in keloidand hypertrophic scar were 75.0％ (15/20),60.0％ (12/20) and 60.0％ (12/20),50.0％ (10/20),nom-pathologic scars were 20％ (4/20),10％ (2/20);normal skin tissue were 10％ (2/20),5％ (1/20),which were higher respectively compared with the two control groups (P＜ 0.05);there was a highly positive correlation between p-mTOR and p-4EBP1 expression in pathologic scar (r=0.323,P＜0.05).Conclusions p-mTOR and p-4EBP1 might be involved and cooperated in promoting the progress of pathologic scars.

BACKGROUND:Cerebral white matter demyelination is outstanding in the images of delayed encephalopathy after acute carbon monoxide (CO) poisoning. Since Nogo-A and Nogo-receptor are expressed in onoligodendrocytes and neurons respectively, we infer that Nogo-A system is involved in brain injury after acute CO poisoning and related to delayed encephalopathy after acute CO poisoning. Endogenous CO is a gaseous messenger, which is the metabolic product of hemeoxygenase. There is no report about the CO effect on Nogo-A system til now. OBIECTIVE: To in vitro culture oligodendrocytes using endogenous CO, inhibit the activity of hemeoxygenase system using zinc protoporphyrin-IX (ZnPPIX) and observe the variation of Nogo-A in oligodendrocytes at mRNA and protein levels. METHODS: Rat oligodendrocytes cultured in vitro were divided into control, CO, ZnPPIX groups. Cels in the CO and ZnPPIX groups were treated with 1% CO directly, In the ZnPPIX group, 10 μmol/L ZnPPIX was added into the culture medium before CO treatment. The expressions of Nogo-A mRNA and protein at 6, 24, 48 hours after culture were compared. Differences in the peak levels of Nogo-A mRNA and protein between CO and ZnPPIX groups were detected using RT-PCR and immunohistochemistry respectively. RESULTS: The expression levels of Nogo-A mRNA and protein were significantly higher in the CO group than the control group and reached the peak at 24 hours of culture. Compared with the CO group, oligodendrocytes cultured with ZnPPIX showed higher expressions of Nogo-A mRNA and protein at 24 hours of culture. These findings suggest that except the influence of hypoxia occurring in CO poisoning, exogenous CO increases the expression of Nogo-A in cultured oligodendrocytes in vitro, and the heme oxygenase system can inhibit the expression of Nogo-A mRNA and protein.

Aim Toinvestigatethepro-angiogenic effects of Danshensu derivative ADTM and explore its underlying possible signaling pathway using zebrafish embryosasinvivomodels.Methods Theangiogenesis activities of ADTM were determined in experimental models of normal and VEGFR tyrosine kinase inhibitorⅡ(VRI )-induced vascular defective zebrafish embry-os.Embryos were treated with various concentrations (50,100,200 μmol · L-1 ) of ADTM for indicated time.The diameter and the numbers of endothelial cells of zebrafish SIVs were evaluated,respectively.In VRI model,the number of intact and defective ISVs in each zebrafish embryo was counted.The total RNA of zebrafish embryos was extracted and transcriptional profiling was analyzed by deep sequencing.Quantita-tive real-time PCR(qPCR)was performed to 4 genes selected from transcriptional profiling to validate the data collected from transcriptome analysis.Results ADTMsignificantlyincreasedsubintestinalvessels (SIVs)diameter in a concentration-dependent manner in normal zebrafish as well as restored VRI-induced blood vessels defect in VRI-exposed zebrafish. The transcriptome data analysis demonstrated that 19 signif-icantly changed genes were mapped to insulin signaling pathway.The qPCR data are in good agreement with those obtained by deep sequencing and support the consistency between the two methods for determining relative expression levels in the zebrafish model.Con-clusion Inzebrafishmodel,ADTMexhibitsthe effects of angiogenesis and blood vessel restoration. The underlying mechanism may be involved in the acti-vation of insulin signaling pathway.

Objective:To evaluate the rapid nucleic acid amplification detection of Mycoplasma pneumoniae (MP)-DNA and MP-RNA in the diagnosis of MP infection and therapeutic values in children. Methods:Patients who were diagnosed with pneumonia were enrolled from the Department of Respiration, Children′s Hospital of Capital Institute of Pediatrics from January 2018 to December 2018.Specimens were detected using the MP and Macrolide-Resistant isolates Diagnostic Kit (PCR Fluorescence Probing, Jiangsu Mole Bioscience Co., Ltd.) and MP Diagnostic Kit (Isothermal RNA amplification, Shanghai Rendu Biotechnology Co., Ltd.).Results:Among them, 42.1%(840 cases) of the 1 994 cases were positive for MP-DNA, and the macrolide associated gene mutations were detected in 96.0% (806/840 cases) of them, while 33.9% (551 cases) of 1 624 cases were positive for MP-RNA.Seven hundred and fifty-eight specimens were simultaneously detected by adopting MP-DNA and MP-RNA, and the positive rate was 43.1% (327/758 cases) and 36.7% (278/758 cases), accordingly, which were inconsistent (Kappa=0.604) in 613 (80.9%, 613/758 cases) cases, with significant differences ( χ2=6.60, P=0.01). Part of the specimens were rechecked with the interval of 7 days: MP-RNA was negative in 70.1% (47/67 cases) specimens and MP-DNA was negative in 36.1% (22/91 cases) specimens ( χ2=33.20, P<0.01). Conclusions:The positive detection rate of MP was at a high level in 2018, in Beijing, China.The results of MP-DNA and MP-RNA are consistant.But RNA detection can help to diagnose MP in the early stage, and monitor the survival of MP and its efficiency.