Objective To investigate the effects of the failure rate of laparoscopic apparatus with power through detecting the electrical properties before packaging it. Methods The laparoscopic apparatus with power used in the operation in January to June 2014 were randomly selected and divided into the control group and the observation group according to whether they underwent electricity performance detection. The control group did not receive testing of electrical properties before packing. The observation group tested the electrical conductivity of the single or double pole conductor, unipolar electric coagulation hook, bipolar electric coagulation forceps, the handle of electric knife by digital multimeter and the performance of the uterus rotary cutting machine by electric hysterectomy controller before packing. The difference of equipment in the number of failures, the type of failure, the number of cases and the satisfaction of medical personnel were observed between the two groups. Results In the observation group, 12 cases with unipolar and bipolar coagulation failure, 10 cases with electrocision handle failure, 2 cases with uterine rotary- cutting failure, 18 cases with adverse conductive or non-conductive, 6 cases with short circuit, which were significantly lower than those in the control group (36,25, 11, 48, and 26 cases), the differences were statistically significant (χ2=5.627-12.063, P<0.05). The satisfaction degree in the observation group was 94.74% (36/38), which was better than 76.32%(29/38) of the control group, the difference was statistically significant (Z=-2.267, P<0.05). Conclusions Testing the electric performance of laparoscopic apparatus with power before packing could reduce the incidence of intraoperative equipment failure rate, This method can provide safe and high quality surgical instruments.

ObjectiveTo explore the effect of health education on uncertainty in illness and hope level of patients with acute coronary syndrome.Methods100 patients with acute coronary syndrome were randomly divided into the control group and the observation group with 50 patients in each.The control group received routine treatment,the observation group was given health education based on routine treatment.After treatment Mishel Uncertainty in Illness Scale(MUIS)and Herth hope index(HHI)scale were used for effect evaluation.ResultsThere were no significant differences on MUIS and HHI scores between two groups before nursing intervention.But after nursing intervention,MUIS score was lower and HHI score was higher in the observation group than those of the control group.ConclusionsHealth education on patients with acute coronary syndrome can reduce the uncertainty in illness and improve the level of their hopes.

Objective To observe the effect of humanistic care on blood pressure control of elderly patients with hypertension. Methods 138 elderly patients with hypertension patients in our hospital were randomly divided into the intervention group and the control group with 69 cases in each group, the intervention group received anti-hypertensive medication as well as the system of nursing intervention,and the control group only was given simple drug treatment. The blood pressure control results were compared. Results SCL-90 factor of the intervention group was significantly higher than the Chinese module before intervention, and SCL-90 factors after intervention was significantly lower than before intervention. In the intervention group, the total effective rate of blood pressure control was 92.75%, significantly higher than the control group. Conclusions Humanistic nursing interventions to elderly patients with hypertension with various forms and more ways can effectively control the blood pressure, improve the therapeutic effects, improve nursing satisfaction degree and quality of care and is worthy of promotion and application.

Objective To explore whether an individual' s intention to gamble varied across different types of gambling. Methods A total of 373 Macau students completed a questionnaire survey on intention to gamble dealing with thirteen types of gambling,and exploratory factor analyses were conducted. Results The analyses showed two factors that had eigenvalues greater than one and explained a total of 59. 455 percent of the variance,with the largest factor explaining 32.59 percent. Participants' intentions to gamble were relatively lower in gambles of low counterparty with mean scores varying from ( 1.62 ± 1.13 ) to ( 2.20 ± 1.35 ), while the intentions were higher in gambles of high counterparty with mean scores varying from ( 2.34 ± 1. 39 ) to ( 3.02 ± 1.55 ). Conclusion Respondents' degree of intention to gamble was highly domain-specific, varying with the type of gambling.An individual' s intention to gamble is not consistent across all content domains, which implied that a potential gambler is not necessarily intended to gamble in all types of gambling.

Objective To compare the antitumor effect of self-developed albumin bound paclitaxel for injection ( PAB) and commercial albumin-bound paclitaxel for injection ( Abraxane ) . Methods The antineoplastic effects of PAB and Abraxane were evaluated in H22, Lewis and RM-1 allograft tumor mouse models after repeated intravenous injection (13. 4, 20. 0, 30. 0 and 45. 0 mg·kg-1 PAB and 20. 0 and/or 30. 0 mg·kg-1 Abraxane, respectively). Results PAB significantly inhibited H22 tumor growth at from the doses of 13. 4, 20. 0, 30. 0, and 45. 0 mg·kg-1,and the antitumor effect of PAB at 20. 0 mg·kg-1 was not significantly different from Abraxane at 20. 0 mg·kg-1 . PAB dose-dependently inhibited Lewis and RM-1 tumor growth at the doses of 20. 0, 30. 0, and 45. 0 mg·kg-1 . The no observed adverse effect level of PAB and Abraxane was 20. 0 mg· kg-1 in Lewis and RM-1 bearing C57 female mice. The antitumor effect and toxicity was not significantly different between PAB and Abraxane at equivalent doses. Conclusion At the same dose level, the antitumor activity and toxicity of PAB was equivalent to those of Abraxane.

Objective To evaluate the value of the new ovarian function marker serum anti-Mullerian hormone (AMH)and to clarify the effect of hysterectomy on the ovarian function of the younger women.Methods The serum AMH,follicle stimulating hormone (FSH)and luteinizing hormone (LH)levels in 35 women suffered uterus benign lesion aged 36-45 years and 35 women suffered hysteromyoma without operation were measured at different time.And the ovarian arterial blood flow resistance index (RI ) was measured by Doppler ultrasound. Results Compred with before operation, the serum AMH levels of the patients in hysterectomy group 2 d and 3 months after operation were significantly decreased (P<0.05 or P<0.01).Compared with control group,the serum AMH levels of the patients in hysterectomy group 2 d and 3 months after operation were also decreased (P<0.05).The ovarian arterial blood flow RI of the patients in hysterectomy group 1 and 3 months after operation were increased compared with control group (P<0.05).There were no significant differences in the serum FSH and LH levels in each group between different time points (P>0.05).Conclusion Hysterectomy can affect the ovarian function,and the serum AMH level 3 months after operation is decreased and the ovarian arterial blood flow RI is increased.AMH is superior to FSH or LH in evaluating the changes of ovarian function.

OBJECTIVETo investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development.

METHODSSpent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed.

RESULTSIn the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02).

CONCLUSIONELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.

Biomarkers ; chemistry ; Chorionic Gonadotropin ; chemistry ; Culture Media ; chemistry ; Embryo Transfer ; Embryonic Development ; Fertilization in Vitro ; Humans

Objective:To evaluate the relationship between hippocampal macrophage polarization and perioperative neurocognitive disorders in mice with tibial fractures.Methods:Forty-five clean-grade healthy male C57/BL6 mice, aged 5-7 months, were divided into 3 groups ( n=15 each) using the random number table method: control group (group C), anesthesia group (group A) and anesthesia surgery group (group AS). Group C received no treatment. Group A was anesthetized with isoflurane inhaled for 15 min. In AS group, intramedullary nail fixation of tibial fracture was performed under anesthesia through inhalation of 2% isoflurane. Morris water maze test and open field test were performed before anesthesia/on 1 day before surgery and after anesthesia/on 1, 3 and 7 days after operation. Five mice were randomly selected after the behavioral experiments were completed at each time point, and hippocampal tissues were taken after the animals were sacrificed for determination of the expression of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), IL-6, chemokine (c-c motif) ligand 12 (CCL2), CCL5, CCL8, iNOS and Arg-1 mRNA (by quantitative real-time polymerase chain reaction), expression of iNOS and Arg-1 proteins (by Western blot), and percentage of CD11b, CD45, CD86 and CD206 cells in hippcampal area (by immunofluorescence staining). Results:Compared with group C, the escape latency was significantly prolonged after operation, the number of crossing the platform was reduced after operation, the expression of TNF-ɑ, IL-6, CCL5 and CCL8 mRNA and iNOS protein and mRNA was up-regulated, the expression of Arg-1 protein and mRNA was down-regulated, the percentages of CD11b + CD45 + cells and CD11b + CD86 + cells in the hippocampus were increased, and the percentages of CD11b + CD206 + cells were decreased in AS group ( P<0.05), and no significant change was found in the parameters mentioned above in group A ( P>0.05). Compared with group A, the escape latency was significantly prolonged after surgery, the frequency of crossing the platform was reduced after surgery, the expression of iNOS mRNA was up-regulated, the percentages of CD11b + CD45 + cells and CD11b + CD86 + cells were increased, and the percentages of CD11b + CD206 + cells were decreased in AS group ( P<0.05). Conclusions:The occurrence of PND may be related to increased polarization to M1 macrophages in the hippocampus and decreased polarization to M2 macrophages in mice with tibial fracture, which further leads to central inflammatory responses.

Objective To investigate the effects of particle size, wind speed and dumping velocity on aerosol concentration distribution during powder dumping in a reprocessing plant. Methods CeO₂ powder was selected as the substitute material of PuO₂. FLUENT software was used to calculate the pouring process of CeO₂ powder under different operation conditions. Then the aerosol concentration distribution under different dumping speeds was measured by particle size spectrometer to verify the accuracy of simulation results. Results The particles with small particle size are more likely to be separated from the mainstream area by the drag force of the surrounding gas, and the radius of the diffusion range also increases with the decrease of the particle size. 2) When the ventilation speed is less than 1 m/s, the dust lifting can be reduced and the concentration of dust particles in the chamber can be reduced to a certain extent. 3) In the process of powder dumping, the spoon is rotated anticlockwise at a speed of 100° in 2～3 s, and less dust aerosol is produced on the right side of the tray. Conclusion When operating the powder particles with smaller particle size, more attention should be paid to the monitoring of aerosol at different positions; the change of air inlet velocity makes the flow field in glove box more complex, and the volume fraction of dust particles is related to the size and location of vortex formed by airflow. The greater the wind speed, the greater the impact on the powder dumping process. The experimental results are basically consistent with the simulation results, and the results show that the lower the dumping speed, the smaller the aerosol concentration near the tray.

Objective: To investigate current situation communication ability and training needs of standardized training nurses, in order to provide reference for improving the communication courses. Methods: A cross-sectional study was conducted and randomized sampling method was used to choose 100 nurses who participated in standardized training in Chengdu Fifth People′s Hospital. Nurses' communication ability was evaluated by Nurse-Patient Communication Ability Scale, and whose training needs of communication were investigated. Results: The average score of the Nurse-Patient Communication Ability Scale was 140.43±13.94. The educational background (t=-3.717, P<0.01), standardized training time (t=-2.812, P<0.01), and whether they had received communication training in the last year (t=2.231, P<0.05) had significant influence on nurse-patient communication ability. 88.00% (88/100) thought it necessary to carry out nurse-patient communication ability training during standardized training. Among them, 88.64% (78/88) hoped to carry out humanistic knowledge training, 65.91% (58/88) hoped that at least four training methods would be combined, and 44.32% (39/88) hoped that teachers from multiple disciplines would conduct training. Conclusions The nurse-patient communication ability of standardized training nurses needs to be improved. It is necessary to strengthen the theoretical research and curriculum design of training, pay attention to practical teaching, so that the training effect can be improved.