Objective To investigate the expression of adrenomedullin (ADM) and vascular endothelial growth factor( VEGF) in plasm of patients with lung cancer,their correlation,and clinical significance. To explore the potential value of ADM for lung cancer in clinic. Methods The levels of plasms ADM and VECF were measured by means of radioimmunoassay in 66 cases of lung cancer, 31 cases of benign lung disease and 16 cases of healthy persons.Results The expression levels of plasm ADM and VEGF were significantly higher in the patients with lung caner than those in benign lung disease and healthy persons (P <0.05) , ADM in benign lung disease was significantly higher than that in the healthy persons (P <0.05), while VEGF in benign lung disease and healthy persons had no significant difference (P >0.05). The levels of plasm ADM and VEGF in distant metastasis was significantly higher than those with local lymph node metastasis ( P < 0.05 ) ,local lymph node metastasis was significantly higher than those without lymph node metastasis (P <0. 05). The levels of plasm ADM and VEGF were closely related to the local lymph node metastasis, distant metastasis and TNM staging. Furthermore, the expression levels of plasm ADM and VEGF increase with the clinical stage (P < 0.05 ). There was a significantly positive correlation between ADM and VEGF in patients with lung cancer (r = 0.449, P < 0.01) , but there was any correlation between benign lung disease group and healthy group ( P > 0. 05 ). Conclusion ADM and VEGF have synergistic effects in the process of neovascularization in lung cancer; to detect the expression of ADM, VEGF in plasm may be helpful for diagnosis of lung cancer and benign lung, lung cancer clinical staging,to interfere with ADM and its signal transduction pathway may be another new targeted therapy of lung cancer .

Objective To discuss oral cavity nursing in senile diabetic patients with oral disease.Methods 80 senile diabetic patients were offered diet control and treatment for lowering blood glucose with simultaneous oral nursing for 4 weeks.The blood glucose,blood lipid before and after nursing were observed.Results The total effective rate reached 91.3% after oral nursing,blood glucose and blood lipid greatly lowered after nursing.Conclusions Prevention of oral disease is important for preventing diabetic complications,appropriate oral nursing contributes to prevention and treatment of diabetes mellitus complicated with oral disease.

Objective To understand the variation of the motality and death causes for diabetic inpatients in West China Hospital(WCH) during 1996～2004. Methods First,the amount of diabetic inpatients(14670) and died persons (9597)were got each year by computer search system of our hospital from 1996 to 2004,and the case code of died diabetic subjects (842)were obtained.Then,those medical records were investigated and reviewed in details to get useful information.The motality,death causes and its constituent ratio of dead diabetic inpatients were analysed. Data input and analysis were conducted using the tool Epidate3.0,packages SPSS13 and SAS8.0. Results There were totally 14760 inpatients and 842 deaths who were suffered from DM during 1996-2004.The mortality rate was 5.74% and the standardized mortality rate was 5.37%.Tumor was the first cause of deaths (22.3%),followed by infection(15%),fatal cardiovascular events(14.4%),cerebrovascular events(12.3%).Diabetic nephropathy(DN)(6.3%) took the 6th place just after COPD(8%).There were 9269 deaths in the hospital in total(≥15 years old) during 1996-2004 while DM patients contributed 9.08% to them.The proportion of diabetic patients among the total deaths kept increasing every year(χ2=71.9070,P<0.01). Conclusions The mortality proportion of diabetic inpatients among the total deaths in West China Hospital from 1996 to 2004 is increasing every year.It needs to pay more attention to the main death cause of tumor in diabetic patients

Objective: To research three-dimensional computed tomography angiography (3D-CTA) combined three-dimensional digital subtraction angiography (3D-DSA) in the diagnosis of intracranial aneurysms. Methods: 3D-CTA and 3D-DSA were performed in 27 cases with Highly suspected in patients with intracranial aneurysms, And compared with the final outcome of surgery. To evaluate 3D-CTA and 3D-DSA in the detection rate of aneurysms, three-dimensional shape and spatial relations ability to display. Research 3D-CTA combined 3D-DSA in the diagnosis of intracranial aneurysms. Results: Twenty-seven cases of suspected patients, 25 cases of surgical treatment of confirmed aneurysms, 28 aneurysms found. 3D-CTA and 3D-DSA rate of aneurysms was no significant difference. 3D-CTA of the aneurysm can be accurately detected clearly shows its three-dimensional shape and spatial relationship with the surrounding skull, 3D-DSA for aneurysm neck, the tumor and the aneurysm and parent artery relationship shows clear and accurate. Conclusion: Imaging of intracranial aneurysm diagnosis, 3D-CTA and 3D-DSA have their respective advantages, combined two methods can improve the detection rate of aneurysms. 3D-CTA combined 3D-DSA have support of design for operation.

Objective To explore the effect of behavioral intervention on the quality of life of chronic heart congestive failure patients with urinary incontinence, to further provide basis for more effective clinical nursing measures. Methods 62 patients with chronic heart congestive failure combining urinary incontinence were divided into two groups by random digital table method, the control group and the intervention group, with 31 cases in each group. Patients in the control group received routine nursing care, while patients in the intervention group received behavioral intervention on the basis of routine care. The behavioral intervention included pelvic floor muscle training, pectineus exercise and reconstruction of micturition habits. The effect of the intervention on incontinence, quality of life and depression were observed in two groups. Results 61 patients completed the study, including 30 cases in the intervention group and 31 cases in the control group. There were no significant differences in the scores of the International Consultation on Incontinence Questionnaire-Urinary Incontinence Short Form (ICI-Q-SF), the MOS item Short Form Health Survey (SF-36) and Zung Self-rating Depression Scale ( SDS) between two groups before the intervention (P>0.05). After 6 weeks of continuous intervention, the scores of ICI-Q-SF and SDS in the intervention group were(8.69±1.94)points and (55.91±4.57) points, which were significantly lower than (11.07±2.14) points and (61.44±5.98) points of the control group (t=5.04, 3.18, P<0.01). About the SF-36, scores of physical functioning and social functioning in the intervention group were (76.77 ±10.34) points and (77.69±6.17) points, which were significantly higher than (60.39±10.07) points and (59.38±8.25) points in the control group (t=43.31, 120.36, P<0.01). Conclusions For chronic heart congestive failure patients with urinary incontinence, behavioral intervention can effectively ameliorate symptoms of urinary incontinence, improve the quality of life and relieve patients' depression, which thereby potentially promote patients′physical and mental health.

Medical imaging plays a more important role in diagnosis and treatment of diseases with the rapid development of big data technologies and hospital information systems.It is thus quite necessary to develop the effective and accurate medical imaging retrieval systems.The key techniques for text-based, contents-based and semantic-based medical imaging retrieval methods were thus elaborated and analyzed with the prospects put forward for the future development direction of medical imaging retrieval techniques.

OBJICTIVE: To prepare Breviscapine orally disintegrating tablet and to establish its quality control method. METHODS: The formula of the core of the tablet was optimized with disintegration time as a parameter by an orthogonal design, and the content and the related substances of Breviscapine orally disintegrating tablet were determined by HPLC. RESULTS: The prepared Breviscapine orally disintegrating tablet showed a good stability, and its characters, dissolution, content and the related substances were all in conformity with the related specifications stated in Chinese Pharmacopoeia (2005 edition). CONCLUSION: The methods of preparation and Quality control are simple and feasible.

Wearing transfemoral prosthesis is the only way to complete daily physical activity for amputees. Motion pattern recognition is important for the control of prosthesis, especially in the recognizing swing phase and stance phase. In this paper, it is reported that surface electromyography (sEMG) signal is used in swing and stance phase recognition. sEMG signal of related muscles was sampled by Infiniti of a Canadian company. The sEMG signal was then filtered by weighted filtering window and analyzed by height permitted window. The starting time of stance phase and swing phase is determined through analyzing special muscles. The sEMG signal of rectus femoris was used in stance phase recognition and sEMG signal of tibialis anterior is used in swing phase recognition. In a certain tolerating range, the double windows theory, including weighted filtering window and height permitted window, can reach a high accuracy rate. Through experiments, the real walking consciousness of the people was reflected by sEMG signal of related muscles. Using related muscles to recognize swing and stance phase is reachable. The theory used in this paper is useful for analyzing sEMG signal and actual prosthesis control.
Artificial Limbs ; Electromyography ; Humans ; Leg ; Muscle, Skeletal ; physiology ; Walking ; physiology

OBJECTIVE: To investigate the uptake of self-assembled sodium alginate(SA) nanoparticles labeled by FITC(sSAN-FITC) in Caco-2 cells and the influencing factors.METHODS: Fluorescence microscope was utilized to exam the uptake of sSAN-FITC in Caco-2 cells.Multifunction continuous spectrum enzyme-linked immunosorbent assay system was used for the detection of the fluorescence intensity of FITC in the cells and quantitative assay of the effect of sSAN concentration,its action time and pH on the uptake of sSAN-FITC in Caco-2 cells,with blank group as control.RESULTS: Pictures of Caco-2 cells taken by fluorescence microscope showed uptake of sSAN-FITC by Caco-2 cells.As compared with blank control group,among the 3 factors,the fluorescence intensity increased with the increase of the sSAN concentration and the prolonging of the action time(P

Background and purpose:Nowadays, the golden standard of diagnosis for malignant hydrothorax or ascites is exfoliocytology examination, but the missed diagnosis rate is too high. Other methods including immunologic test, telomerase activation test, conjugation of chromosome analysis with cytological examination test, and RT-PCR test. But none of them was widely used due to high cost or high false positive rate. Immunomagnetic beads (IMB) technique is a popular method all over the world in recent years. It was mainly used in isolating and purifying cells, but it was rarely used to detect cancer cells in patients with hydrothorax or ascites so far. Our aim was to fi nd an effi cient way to detect the cancer cells in patients with cancer related hydrothorax or ascites and to improve the corresponding diagnosis rate. Methods:In the experiments, both the traditional exfoliocytology examination method and IMB technique were used to detect the cancer cells in the hydrothorax or the ascites for comparison. Results:Using IMB technique and exfoliocytology method, the positive rates in 30 patients with cancers were 63.3% (19/30) and 23.3% (7/30), respectively, and the false positive rates in 30 patients without cancers were 3.3% (1/30) and 0.0% (0/30), respectively. It could be observed that the positive rate using IMB technique was much higher than that using exfoliocytology method (P0.05). Conclusions:The present study demonstrated that IMB technique is an accurate, sensitive, fast and economic method in detecting the cancer cells in patients with cancer related hydrothorax or ascites, especially for diagnosis and therapy in the early clinical stage. Due to the high effi ciency, IMB technique could be used after exfoliocytology examination to improve the diagnosis rate..