1.Effects of rosuvastatain on apolipoprotein A1 and B of patients with acute coronary syndrome
Lingji ZHANG ; Yanli YANG ; Hong YAN
Clinical Medicine of China 2013;(4):367-369
Objective To observe the effect of Rosuvastatin on apolipoprotein A1 (Apo A1),apolipoprotein B(Apo B) and Apo B/Apo A1 patients with acute coronary syndrome.Methods One hundred and fifty hospitalized patients with acute coronary syndrome were randomly divided into three groups,A group (Rosuvastatain,5 mg/d),B group (Rosuvastatain,10 mg/d),and C group (Simvastatin,20 mg/d).Before and at 4,8 weeks after treatments tested the three groups' cholesterol (TC),glycerin(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol (LDL-C),Apo A1,Apo B.Compare the blood lipids aforementioned before and after treatments in three groups.Results (1) After treatments for 4 weeks,TC,LDLC in three groups are significant lower than before (P < 0.05).After treatments for 8 weeks,TC,LDL-C,Apo B,Apo B/Apo A1 of three groups are lower than before and HDL-C 、Apo A1 is higher than before (P < 0.05).(2)After treatments for 8 weeks,comparing to A and C groups,TC,LDL-C,Apo B,Apo B/Apo A1 in B group were lower while HDL-C、Apo A1 were higher (P < 0.05).(3)The incidences of liver damage has no significant differences among three groups (x2 =1.25,P > 0.05).Conclusion Effects of 10 mg/d rosuvastatain were better than 5 mg/d rosuvastatain and 20 mg/d simvastatin in increasing Apo A1 and decreasing Apo B,Apo B/Apo A1,and then effectively control of ACS.
2.Advance in Cluster Needling of Scalp Acupuncture Combined with Rehabilitation for Stroke (review)
Yanli XING ; Yan WANG ; Luwen ZHU
Chinese Journal of Rehabilitation Theory and Practice 2011;17(4):304-306
The researches that stroke was treated by cluster needling of scalp acupuncture combined with rehabilitation in the past 10 years were reviewed, noting that it can significantly reduce the dysfunctions in stroke patients, prompting that the cluster needling of scalp acupuncture and modern rehabilitation techniques was combined, was a new method for stroke rehabilitation treatment.
3.Investigation and analysis about parturition related knowledge of patient-controlled epidural analgesia among pregnant women
Limin WANG ; Meiqin YAN ; Yanli HU
Chinese Journal of Practical Nursing 2015;31(12):889-892
Objective To know the awareness level of patient-controlled epidual analgesia in labor related knowledge,and to provide evidence for health education,develop a comprehensive intervention program to reduce the rate of cesarean section.Methods Investigation on knowledge of patient controlled epidural analgesia during labor for a total of 400 pregnant women on Maternal and Child Health Hospital of Taiyuan City,MCH Hospital of Shanxi Province by questionnaire.Compared different characteristics of the pregnant women's scores about control epidural analgesia childbirth knowledge by nonparametric test,knowledge score of multi-factor analysis by multiple linear regression analysis.Results The maximum score of pregnant women-controlled epidural analgesia delivery knowledge was 13,the minimum score was 0,the median score was 4,range interquartile was 5.Different pregnant women with different age,education level,number of weeks of gestation,pregnancy,family income,and condition of parnatal care had different scores of knowledge of patient-controlled epidural analgesia,the difference was significant,P<0.05.Multiple linear regression analysis showed that:pregnant women's education,gestational age,the average monthly household income can be explained by maternal knowledge score of 61.4% of the variation.Conclusions The level of pregnant women-controlled epidural analgesia childbirth knowledge is low,we need to strengthen the natural childbirth education,and take a variety of modes of propaganda and increase their level of knowledge,enhance confidence of natural childbirth in pregnant women,so as to achieve the purpose of reducing cesarean section rate.
4.Construction of goat germ cell specific reporting system pVASA-EGFP.
Guangyao YAN ; Peizhen LI ; Caifang REN ; Feng WANG ; Yanli ZHANG
Chinese Journal of Biotechnology 2015;31(9):1313-1324
To monitor the trans-differentiation from adult stem cells to germ cells, we analyzed the vasa expression of goat testicular tissues in different ages and constructed the germ cell specific reporting vector pVASA-EGFP. The expression of vasa was verified by RT-PCR and immunofluorescence. The vector pVASA-EGFP was constructed by molecular technology, then transfected into goat bone mesenchymal stem cells (BMSCs) by Lipofectamine 2000. Moreover, we observed the expression of the vector through green fluorescent protein (GFP). Immunofluorescence results show that Vasa was expressed in all groups of goat testicular tissues, RT-PCR results show that the levels of vasa mRNA in 3-month group and 10-month group were significantly higher than that in 10-day group. Sequencing and restriction enzyme results show that the vector was successfully constructed. After transfection and RA treatment, GFP expression was observed, which proved the validity of our reporting system. All the results proved that vasa was expressed in different ages in goat testicular tissues, and the vector pVASA-EGFP is efficient in monitoring the trans-differentiation in vitro, which paves the way for further characterization and screening of the trans-differentiation of goat BMSCs.
Animals
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Cell Transdifferentiation
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Genes, Reporter
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Genetic Vectors
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Germ Cells
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cytology
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Goats
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Green Fluorescent Proteins
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Male
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Mesenchymal Stromal Cells
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RNA, Messenger
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Testis
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metabolism
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Transfection
5.Clinical significance of N-terminal pro-B-type natriuretic peptide detection in evaluation of acute type A aortic dissection prognosis
Yanli LIU ; Tingting LI ; Yan WANG ; Li DING ; Yajun SONG
International Journal of Laboratory Medicine 2014;(9):1119-1121
Objective To investigate the clinical significance of N-terminal pro-B-type natriuretic peptide(NT-proBNP) detection in evaluation of acute type A aortic dissection (AAAD) prognosis .Methods Medical records of patients with confirmed diagnosis of AAAD were retrospectively analyzed .64 patients were enrolled in accordance with the inclusion and exclusion criteria ,and were di-vided into survival group(n=56) and death group(n=8) according to prognosis .Multivariate Logistic regression analysis was em-ployed to screen the independent risk factors which would affected the prognosis .Receiver operator characteristic ,ROC) curve was used to evaluate the predictive value of NT-proBNP for AAAD prognosis .Results Differences of history of smoking ,incidence sea-son ,disturbance of consciousness ,misdiagnosis ,low density lipid-cholesterol(LDL-C) ,fasting C-peptide(FCP) ,hypersensitive C-re-active protein(hs-CRP) ,cardiac troponin I(cTnI) ,NT-proBNP ,mean arterial pressure(MAP) and prothrombin time(PT) of pa-tients between the survival group and the death group showed statistical significance (P<0 .05) .Multivariate Logistic regression a-nalysis demonstrated that smoking history ,disturbance of consciousness ,high LDL-C ,high cTnI ,high NT-proBNP and low MAP were independent risk factors for patients with poor prognosis .ROC area under the curve(AUC) of NT-proBNP prediction for peri-operative mortality risk of patients with AAAD was 0 .697(P< 0 .05 ,95% CI:0 .643~0 .733) ,and its specificity and sensitivity were 75 .85% and 70 .07% ,respectively .When NT-proBNP was 909 .69 pg/mL ,its predictive value for AAAD was the best .Set the critical value as the threshold ,the patients above were divided into the threshold above group and the threshold below group . The survival rate of patients in the threshold below group [98 .18% (54/55)] was significantly higher than that in the threshold a-bove group[22 .22% (2/9)](χ2 = 7 .211 ,P< 0 .05) .Conclusion NT-proBNP level is closely related to the prognosis of patients with AAAD and NT-proBNP detection may be conducive to predicting high risk of AAAD .
6.EFFECT OF DRIED BIOOD POWDER FORTIFIED BISCUIT ON PREVENTION OF IRON DEFICIENCY ANEMIA
Xiuyun LIU ; Leyi YAN ; Xiaoming YU ; Furu LI ; Yanli SU
Acta Nutrimenta Sinica 1956;0(02):-
The amounts of iron and amino acids in dried pig powder were determined. It contained a large quantity of iron (230-260m/100g) and all essential amino acids. The blood fortified wheat flour biscuit containing 15mg Fe/100g was made and 30?2g of the biscuit was given to 65 primary school students (age 8-10) per capita per day during school day break for 40 days from Sep to Oct in 1984. For the control group, equal amount of the same biscuit without blood fortification was given to 34 subjects of the same age. Finger blood was taken for determination of hemoglobin, hematocrit, RBC count and serum ferritin before and after the experiment.there were significant differences (P
7.Matrine sustained release tablet, capsule and injection: comparison of pharmacokinetics and relative bioavailability
Yan LI ; Weiwei CAO ; Yanli YANG ; Hao ZOU ; Xueta JIANG
Academic Journal of Second Military Medical University 1985;0(06):-
Objective:To compare the pharmacokinetics and relative bioavailability between matrine sustained release tablet,matrine conventional capsule and matrine injection. Methods: Dogs were given single oral dose and multiple doses of matrine sustained release tablet, capsule or injection in a randomized crossover way. Matrine concentrations in dog plasma were determined by RP-HPLC method. Results: The results showed that the t max and c max were 300 min and (5.088?0.490) ?g/ml for matrine sustained release tablet, (85?12) min and (6.360?0.215) ?g/ml for the conventional capsule, and 10 min and (6.500?0.404) ?g/ml for the injection. The relative bioavailability of the sustained release tablet was (153.7?9.4)% compared with that of the conventional capsule; the absolute bioavailability of the sustained release tablet was (73.5?14.2)% compared with that of the injecetion. The in vivo absorption rate of the sustained release tablet was significantly correlated with the in vitro release rate(r=0.981 2,P
8.Combined impacts of blood glucose level and glucose metabolism-related factors on liver 18F-FDG uptake
Yan HU ; Guobing LIU ; Yanli LI ; Jie XIAO ; Hongcheng SHI
Chinese Journal of Nuclear Medicine and Molecular Imaging 2017;37(8):470-474
Objective To evaluate the combined impacts of blood glucose and its related metabolic factors on 18F-FDG uptake by liver.Methods A total of 544 subjects (384 males and 160 females, age range 24-73 years) undergoing 18F-FDG PET/CT were recruited in this retrospective study.SUVmean of the right lobe of liver was calculated.Two-sample t test and one-way analysis of variance were performed to compare SUVmean between patients with different genders and BMI levels.Linear correlation analysis, partial correlation analysis and multiple linear regression analysis were conducted to evaluate the relationship between age, injected 18F-FDG dose, blood glucose, serum T3, T4, FT3, FT4, BMR, BMI and liver SUVmean.Results The SUVmean of the liver in males and females were 1.89±0.42 and 1.92±0.38 (t=0.693, P>0.05), but it was significantly different among BMI groups (F=3.056, P<0.05).Age, blood glucose and FT3 were significantly associated with liver SUVmean (r′ values: 0.108, 0.140 and 0.105, all P<0.05) and were independent factors that indicated variation of liver SUVmean (β values: 0.006, 0.070 and 0.088, all P<0.05).Blood glucose was the strongest powerful predicting variable of liver SUVmean (β′=0.154, P<0.001).Conclusions Blood glucose and its related metabolic factors can affect the liver 18F-FDG uptake.Age, FT3, blood glucose are independent factors predicting variation of liver SUVmean.The impact of glucose metabolism status should be considered when assessing liver 18F-FDG uptake.
9.Effects of different kinds of opiate receptor on oxycodone-induced hyperlococomotor and analgesia in mice
Yanli LIU ; Lingdi YAN ; Chunfu WU ; Zehui GONG
Chinese Pharmacological Bulletin 2003;0(11):-
Aim To investage the effects of ?、?、?-receptor on hyperlocomotor and analgesia in mice.Methods Locomotor activity experiment and hot-plate pain experiment were measured.Results Oxycodone dose-dependently enhanced locomotor response in mice.Naloxone,a unselective opioid receptor antagonists and naltrindole(?-selective opioid receptor antagonist)could attenuate the increase of locomotor acitivity induced by oxycodone,but naloxonazine,a ?-receptor antagonist had no such effect.?-receptor antagonist nor-Binltorphimine increased hyperlocomotion induced by oxycodone.In antinociceptive experiment,naloxonazine and naltrindole could not attenuate the that of oxycodone,but nor-Binaltorphimine could attenuate the antinociceptive effects of oxycodone.Conclusion Oxycodone-hyperlocomotor is probably mediated by ? opioid receptor,and antinocieptive effects of oxycodone is probably mediated by ? opioid receptor.
10.Effects of miRNA-181a on proliferation and migration of multiple myelo-ma cells
Xiao YAN ; Yanli ZHANG ; Guifang OUYANG ; Qitian MU ; Lixia SHENG
Chinese Journal of Pathophysiology 2017;33(1):33-37
AIM:To investigate the effect of miRNA-181a inhibition on the proliferation, migration and cell cycle of the human multiple myeloma cell line RPMI 8226.METHODS:Real-time PCR was used to detect miRNA-181a expression in serum samples from multiple myeloma or healthy subjects .After transfection with miRNA-181a inhibitor, the cell viability was examined by CCK-8 assay and colony formation assay .The cell migration ability was analyzed by wound healing assay .The cell cycle was detected by flow cytometry .Moreover , the protein level of cyclin D 1 and the phosphoryla-tion of PI3K and Akt were determined by Western blot .RESULTS:The expression of miRNA-181a was significantly in-creased in the serum from multiple myeloma patients as compared with healthy group .Inhibition of miRNA-181a expression by transfection with miRNA-181a inhibitor remarkably decreased the cell viability , migratory ability, the population of G0/G1 phase and cyclin D1 protein expression in the RPMI8226 cells.However, the population of S phase and the phosphory-lation of PI3K and Akt were reduced .CONCLUSION:Down-regulation of miRNA-181a inhibits the viability and migra-tory ability in the RPMI8226 cells via inhibition of cell cycle and PI 3K/Akt signaling pathway .