Objective To prepare high-quality refined and depolymerized capsular polysaccharide(CPS) of Streptococcus pneumoniae for preparation of polysaccharide vaccine and polysaccharide conjugate vaccine against Streptococcus pneumoniae.Methods Refined CPS of type 6A was prepared by acid precipitation method from the inactivated fermentation broth of type 6A Streptococcus pneumoniae,and the final concentration of CaCl_2(80,160,240,320 mmol/L) and pH(2.5,3.0,3.5,4.0) were optimized.Three batches of type 6A refined CPSs were prepared by the optimized method,and the applicability of the method to polysaccharides of 5,7F,10A and 19F types was verified.After removing impurities from the inactivated broth of type 6 A Streptococcus pneumoniae by acid precipitation,the type 6 A depolymerized CPS was prepared by trifluoroacetic acid method,and the reaction temperature(8,25 and 40℃),the final concentration of trifluoroacetic acid(0.25,0.50,1.00 mol/L) and the reaction time(1,3,6 h) were optimized.Three batches of depolymerized CPSs were prepared by the optimized method,and compared with the high-pressure homogenization method,and then the applicability of trifluoroacetic acid method to depolymerized CPSs of 5,10A and 19F types was verified.The quality of refined and depolymerized CPSs was tested according to the methods specified in Chinese Pharmacopoeia(Vol Ⅲ,2020 edition).Results The optimum final concentration of CaCl_2 and pH of acid precipitation method were 160 mmol/L and 3.0 respectively.The prepared type 6A,5,7F,10A and 19F refined CPSs were all qualified.The optimum reaction temperature,reaction time and final concentration of trifluoroacetic acid were 25℃,3 h and 0.50 mol/L for trifluoroacetic acid method.The prepared type 6A,5,10A and 19F depolymerized CPSs were all qualified.Conclusion The refined and depolymerized CPSs of Streptococcus pneumoniae prepared in this study meet the requirements of Chinese Pharmacopoeia(Vol Ⅲ,2020 edition),and the preparation process is suitable for the preparation of various types of polysaccharides.

C.Conclusion: The drug releasing rate of Icariin Ointment made of emulsion ointment base is the fastest.

The increase of blood-brain barrier (BBB) permeability is a key link in causing the pathological changes in vasogenic brain edema and inflammatory reaction. In recent years, a number of studies have indicaded that a lot of mediators participated in the structural and functional changes and damage of BBB during the process of cerebral ischemia-reperfusion.

There exists different view in the goal of investigative study.Based on the self-fulfilling cycles of self-regulated learning,the author of the article puts forward that developing the ability of self-regulated learning by doing investigative study according to each phase characteristic of self-regulated learning is one of the important goals of investigative study.

Objective To study the tooth whitening effect of boron-dipyrromethene (BODIPY) as a photosensitive drug combining photodynamic therapy (PDT) and the influence on the surface of the enamel.Methods After oxidation of hydrogen peroxide or BODIPY by photodynamic therapy,the extracted teeth were evaluated by Vita shade guides matching.The change of surface enamel on the crystals and microstructure was observed by scanning electron microscopy (SEM).Results The teeth treated with BODIPY by PDT were smooth and whitened with the remineralization of teeth surface in morphology.While the teeth treated with hydrogen peroxide were whitened obviously but the surface morphology of the teeth was demineralized seriously.Conclusion The results suggest that PDT is effective for teeth whitening and can promote the remineralization of teeth surface enamel with BODIPY as a photosensitive drug.PDT is expected to become a new method for tooth whitening.

Objective To observe the correctly positioning rate of left-sided double lumen endobronchial tube by combined adjustment of auscultation,PETCO2 and airway pressure changes.Methods Sixty adult patients undergoing thoracic surgery were intubated with Robertshaw DLTs. DLT position is checked and adjusted by fiberoptic bronchoscope（FOB）. Results In the supine position,successful intubation is 57 cases by three combined methods,one of cases is shallower,two of cases are deeper,satisfactory rate is 95 percents;In the lateral decubitus position,successful intobution is 56 cases by three combined methods,two of cases is shallower,two of cases are deeper,satisfactory rate is 93.4 percents.Conclusion Three combined methods that are simple,reliable can be used repeatedly during postural changes without increasing the opportunities for injury,it is clinically a very good approach,but the absolute scope of safety is very small,FOB is necessary for positioning.

With the deepening of the study on traditional Chinese medicinal quality and the rising requirements of TCM quality control, the demand of setting TCM quality were emphasized,as well as higher education course. Surrounding the purpose of exploring the related issues of the traditional Chinesemedicinal courses setting and promoting the college curriculum innovation capability,the paper mainly discussed the course open orientation, the teaching characteristics, objectives, idea, the core content and the training scheme, as well as the plan of course and teaching material construction depended on these contents.

Objective To study the effect of methyl mercury on the development of rat offspring. Methods The male Wistar rats were divided into four groups randomly, each group was administrated methyl mercury by gavage every two days for two months, then, the rats were mated with healthy female rats. The development, growth and behaviors of offspring were investigated. Results Compared with the control, the body weights of offspring in groups of 1.170 mg/kg and 0.580 mg/kg were less and the development was tardy. The indexes that can reflect the level of development showed that the behaviors of offspring in experimental groups were damaged. Conclusion The male rats exposed to methyl mercury may produce an adverse effect on the development, growth and behaviors of their offspring.

Objective To explore the changes in serum leptin level in the elderly patients with chronic heart failure(CHF) and the relatonship between leptin and blood-pressure. Methods Serum leptin levels were studied in 35 male patients with CHF and 30 male normal controls.The relationship of serum leptin with blood pressure and LVEF were analyzed. Results Compared with controls,patients with CHF had higher serum leptin,syst olic blood pressure(SBP)and cliastolic blood pressure(DBP)(P

Objective:To investigate the expressions of CD44v3 and CD54 in oral premalignant epithelia and its significance in the carcinogenesis of oral mucosa.Methods:The expressions of CD44v3 and CD54 in 19 cases of normal mucosa and mucosal hyperplasia,22 case of dysplastic epithelia,26 cases of oral cancer mucosa were detected by using immunohistochemistry.Results:CD44v3 and CD54 proteins showed no expression or micro-expression in normal and hyperplastic epithelia.The expressions increased in the dysplastic epithelia and mucosa of oral cancer gradually.The difference between the expressions of the three groups was significant(P