Objective To investigate the clinical significance of the detection of the levels of serum uric acid (UA) and serum eytokine tunor necrosis factor-oα (TNF-α),soluble intercellular adhesion molecule-1 (sICAM-1),interleukin (IL)-6 and IL-8 in patients with chronic obstructive pulmonary disease (COPD).Methods UA of 40 healthy people (control group) and 158 COPD patients (COPD group) in acute exacerbation and remission stage was detected respectively by automatic biochemical analyzer,and the levels of serum TNF-α,sICAM-1,IL-6 and IL-8 were detected by ELISA.The percentage of first second forced expiratory volume in expected value (FEV1％) of COPD patients in acute exacerbation and remission stage were detected.Results TNF-α,sICAM-1,IL-6,IL-8 and UA of COPD group in acute exacerbation were significantly higher than those of control group (P＜ 0.01 ),and FEV1％ was significantly lower than that of control group (P＜ 0.01 ).TNF-α,sICAM-1 and UA of COPD group in remission stage were significantly higher than those of control group (P ＜0.01 or ＜0.05),IL-6 and IL-8 had no statistical significance compared with those of control group (P ＞ 0.05),and FEV1％ was significantly lower than that of control group (P ＜ 0.01 ).Correlation analysis showed that TNF- α,sICAM-1,IL-6,IL-8,UA of COPD patients in acute exacerbation were negatively conrelated with FEV1％ (r =-0.681,-0.579,-0.545,-0.652,-0.357,respectively,P ＜ 0.01 or ＜ 0.05 ).In remission stage,TNF- α,sICAM-1,IL-6 and IL-8 of COPD patients were negatively correlated with FEV1％ (r =-0.623,-0.335,-0.349,-0.623,P＜0.01 or ＜0.05),and UA had no significant correlation with FEV1％ (r =-0.092,P ＞0.05).Conclusions The serum UA and cytokines TNF-α,sICAM-1,IL-6,IL-8 are involved in the progression of COPD and correlated with lung function.They can be used as the judge indexes of COPD.

ObjectiveTo explore the clinical application of pleural mesothelial cell (PMC) determination in the diagnosis of pleural effusion.MethodsPMC of 36 patients with malignant pleural effusion (malignant group) and 24 patients with benign pleural effusion (benign group) were counted to determine the clinical value of PMC in benign and malignant pleural effusion.ResultsIn malignant group,there was no case with PMC disappear,1 case(2.78％) with the percentage of PMC between 0 and 1％,1case(2.78％) between 1％ and 5％,34 cases (94.44％) more than 5％.In benign group,there were 11 cases (45.83％) with PMC disappear,8 cases (33.33％) with the percentage of PMC between 0 and 1％,2 cases (8.33％) between 1％ and 5％,3 cases( 12.50％ ) more than 5％.The percentage of PMC between two groups had significant difference (x2 =43.4069,P ＜ 0.01).95％ confidence intervals of PMC in benign and malignant group respectively were 0.28％ -5.10％ and 5.13％ -10.91％,which did not overlap.The percentage of PMC more than 5％ was defined as the diagnosis standard of identifying benign and malignant pleural effusion,the sensitivity of the diagnosis was 94.44％(34/36),the specificity was 87.50％(21/24) and the accuracy was 91.67％(55/60).ConclusionsPMC determination is a kind of easy,economical and fast way to diagnose benignand malignant pleural effusion with high sensitivity,specificity and accuracy.Therefore,it has reference value in clinical diagnosis.

WJ multipurpose guided puncture outfit in interventional ultrasound was developed.Guided puncture with this outfit was performed on 203 cases of various diseases.All 274 punctures were saccessful The study shows hat this guided puncture outfit can be set on various linear probes,fit-ted into puncture,biolpsy or catheter needles.It can actively adjust the puncture angle,precisely forecast,fix and calibrate the puncture angle and depth,perlorm guided puncture in real time etc.It is notnecessary to set line and tabulate the puncture angle.The outfit is easy and rapid to handle and also is reliable with high success rate

Objective To study the effect of calcitriol combined with metformin in the treatment of polycystic ovary syndrome(PCOS)and its influence on serum monocyte chemotactic factor -1(MCP -1).Methods 96 patients with PCOS were randomly divided into the control group(48 cases)and the the observation group(48 cases).The two groups were treated with metformin tablets,0.25g/time,2 times/d.On the basis of the above treatment,the observation group was given calcitriol,0.25μg/time,1 time /d.The two groups were treated for 3 months.Before and after treatment,the number of common symptoms of PCOS(menstrual scarce,acne,hairy,ovarian volume increase,polycystic change)and the determination of serum MCP -1 changes were summarized.Results Before treatment,the menstruation, acne,hairy,ovarian volume increase,polycystic change in control group were 46 cases,21 cases,32 cases,19 cases, 47 cases,respectively,which were 18 cases,11 cases,18 cases,9 cases and 10 cases after treatment.Before treatment, the menstruation,acne,hairy,ovarian volume increase,polycystic change in observation group were 47 cases,25 cases, 29 cases,22 cases,45 cases,respectively,which were 10 cases,6 cases,10 cases,4 cases and 5 cases after treatment. After treatment,the two groups were treated with less menstruation,acne,hair,ovarian volume and polycystic change (χ2 =9.86,7.25,9.06,10.17,13.42;14.28,12.62,11.28,16.52,19.66,all P <0.05).And after treatment, which of the observation group were significantly better than the control group (χ2 =4.06,4.86,5.62,4.71,9.27,all P <0.05).The serum MCP -1 levels of the control group before and after treatment were (159.64 ±27.12)μg/L, (118.54 ±21.38)μg/L,which of the observation group were (162.57 ±29.06)μg/L,(82.59 ±10.72)μg/L.After treatment,the serum levels of MCP -1 in the two groups were significantly decreased(t =2.968 2,4.163 8,all P <0.05),and the serum MCP -1 level in the observation group was significantly lower than that in the control group (t =2.661 2,P <0.05).Conclusion Calcitriol combined with metformin can significantly improve the efficacy of patients with PCOS,and decrease the expression of MCP -1 in serum.

Objective To investigate the prevalence of thalassemia gene carried by child-bearing age people in Meizhou area. Methods Use regular blood tests and hemoglobin electrophoresis to screen the suspect thalassemia patients in couples of child-bearing age who underwent premarital examination or pregnancy check in the hospital.Samples with positive results were tested by using hybridization reverse dot blot to make a definite diagnosis of thalassemia.Results 208 samples were screened to be positive, among which there were 64 cases ofα-thalassemia carrier(30.67%),32 cases ofβ-thalassemia carrier(15.38%),3 case of combina-tion thalassanemia(1.92%).Conclusion The study may provide a reference for taking effective measures to prevent the birth of children with thalassemia.

Objective To study the difference of cerebral infarction patients plasma oxidized low density lipoprotein,circulating endothelial cells,nitric oxide levels and normal people,and study the correlation detection index.Methods 50 patients with cerebral infarction were selected as the observation group,21 cases of normal people were selected as the control group.The oxidized low density lipoprotein,circulating endothelial cells,and nitric oxide were detected.Results The plasma oxidized low density lipoprotein,circulating endothelial cells,nitric oxide levels of the observation group were (687 ±169)μg/L,(9.0 ±1.7)cells/0.9 μL,(52.3 ±16.6)μmol/L respec-tively,which of the control group were (350 ±173)μg/L,(2.5 ±0.5)cells/0.9 μL,(77.4 ±21.1)μmol/L,there were statistifically significant differences between the two groups(t =5.278,8.982,4.371,all P <0.05).Correlation analysis showed that ox -LDL and NO(P =0.013,r =0.782),ox -LDL and CEC(P =0.019,r =0.793),CEC and NO(P =0.021,r =0.952),there were significant correlation.Conclusion Vascular endothelial injury degree will affect the plasma oxidized low density lipoprotein in patients with test results,so the clinical treatment should pay attention to the protection and antiinflammatory treatment on the function of endothelial cells.

GATA4 gene mutations are common in children with congenital heart disease.More than 120 mutations have been found in ventricular septal defect,atrial septal defects and the tetralogy of fallot.But the relationship between genotype and clinical phenotype has not yet been elucidated.This article summarizes the published germline and somatic mutations of GATA4 gene in human congenital heart disease,and provides insight into the phenotypic spectrum of GATA4 gene mutation.

Objective:To observe the effect of GSK-3? on the cell cycle and metastasis of human gastric carcinoma cell.Methods:Influencing the GSK-3? activity of human gastric carcinoma cell line SCG-7901 by LY294002 with or with not LiCl treatment.The expression of total GSK-3? and p-GSK-3?ser9 were detected in protein level by western blotting,cell proliferation and cell cycle distribution were tested by MTT and FCM respectively,the expression of E-cadherin and ?-catenin were measured with S-P immunohistochemistry and the cell-ground substance adhesion was tested by cell adherence assay.Results:The expression of total GSK-3? was constant and the protein level of p-GSK-3? ser9 was down regulated after exposured to LY294002 by 72 h,the cell proliferation was inhibited,cell cycle was arrest at G0/G1 phase,the E-cadherin level was positively regulated,the expression of ?-catenin and the adhesion rate were decreased.The effect on the proliferation、cell cycle、the expression of E-cadherin and ?-catenin and the adhesion by the LY294002 were partially abrogated by lithium chloride.Conclusion:GSK-3?could negatively regulate the proliferation of human gastric carcinoma cell by leading cells to G1-S arrest and inhibit its metastasis by positively regulating the expression of E-cadherin.

Objective:To probe the mechanism of inhibition in gap junction-mediated intercellular communication (GJIC) of rabbit lens epithelia cells(RLECs) irradiated by UVA. Methods:RLECs of 2～3 generation were divided into normal control group and ultraviolet irradiation group (The UV intensity was 0.2 mW/cm2 under a UV lamp with fixed position 50 cm, RLECs were exposed to ultraviolet lamp at different times: 5,10,15 minutes). Western blot techniques were employed to detect the protein levels of Cx43 irradiated by UVA. By indirect immunofluorescence histochemistry we analyzed the localization of Cx43 in the RLECs. Results:In UVA irradiation group and normal control group,there was expression of Cx43. With the increasing intensity,the expression of Cx43 was decreasing. The immunnostaining of Cx43 in RLECs revealed that the internalization of Cx43 from membrance to plasma and nuclear was found in UVA irradiated RLECs. Conclusion: Cell molecule level study proved UVA irradiation can reduce GJIC of RLECs,which wis associated with perturbations in localization and number of Cx43 among RLECs.

Objective:To study the deficiency ofshort stature homeobox containing (SHOX)gene deficiency ofshort stature and the relationship between genotypes and its corresponding phenotypes. Methods:Microsatellite analysis and direct sequencing were selected to analyze the deletions and mutations of SHOX gene in 203 cases of short stature;in addition ,anthropometric measures were assessed and radiographs of the left forearms and wrists in patients were collected to observe their phenotypes. Results:one mutation and eleven deletions were found;Detailed examination revealed certain dysmorphics,such as short forearm and lower leg,et al. Conclusion:In our study,the prevalence of SHOX deficiency in patients with short stature was 5.9% ,and certain correlations had been found between genotypes and corresponding phenotypes。