ObjectiveTo explore the clinical application of pleural mesothelial cell (PMC) determination in the diagnosis of pleural effusion.MethodsPMC of 36 patients with malignant pleural effusion (malignant group) and 24 patients with benign pleural effusion (benign group) were counted to determine the clinical value of PMC in benign and malignant pleural effusion.ResultsIn malignant group,there was no case with PMC disappear,1 case(2.78％) with the percentage of PMC between 0 and 1％,1case(2.78％) between 1％ and 5％,34 cases (94.44％) more than 5％.In benign group,there were 11 cases (45.83％) with PMC disappear,8 cases (33.33％) with the percentage of PMC between 0 and 1％,2 cases (8.33％) between 1％ and 5％,3 cases( 12.50％ ) more than 5％.The percentage of PMC between two groups had significant difference (x2 =43.4069,P ＜ 0.01).95％ confidence intervals of PMC in benign and malignant group respectively were 0.28％ -5.10％ and 5.13％ -10.91％,which did not overlap.The percentage of PMC more than 5％ was defined as the diagnosis standard of identifying benign and malignant pleural effusion,the sensitivity of the diagnosis was 94.44％(34/36),the specificity was 87.50％(21/24) and the accuracy was 91.67％(55/60).ConclusionsPMC determination is a kind of easy,economical and fast way to diagnose benignand malignant pleural effusion with high sensitivity,specificity and accuracy.Therefore,it has reference value in clinical diagnosis.

Objective To investigate the clinical significance of the detection of the levels of serum uric acid (UA) and serum eytokine tunor necrosis factor-oα (TNF-α),soluble intercellular adhesion molecule-1 (sICAM-1),interleukin (IL)-6 and IL-8 in patients with chronic obstructive pulmonary disease (COPD).Methods UA of 40 healthy people (control group) and 158 COPD patients (COPD group) in acute exacerbation and remission stage was detected respectively by automatic biochemical analyzer,and the levels of serum TNF-α,sICAM-1,IL-6 and IL-8 were detected by ELISA.The percentage of first second forced expiratory volume in expected value (FEV1％) of COPD patients in acute exacerbation and remission stage were detected.Results TNF-α,sICAM-1,IL-6,IL-8 and UA of COPD group in acute exacerbation were significantly higher than those of control group (P＜ 0.01 ),and FEV1％ was significantly lower than that of control group (P＜ 0.01 ).TNF-α,sICAM-1 and UA of COPD group in remission stage were significantly higher than those of control group (P ＜0.01 or ＜0.05),IL-6 and IL-8 had no statistical significance compared with those of control group (P ＞ 0.05),and FEV1％ was significantly lower than that of control group (P ＜ 0.01 ).Correlation analysis showed that TNF- α,sICAM-1,IL-6,IL-8,UA of COPD patients in acute exacerbation were negatively conrelated with FEV1％ (r =-0.681,-0.579,-0.545,-0.652,-0.357,respectively,P ＜ 0.01 or ＜ 0.05 ).In remission stage,TNF- α,sICAM-1,IL-6 and IL-8 of COPD patients were negatively correlated with FEV1％ (r =-0.623,-0.335,-0.349,-0.623,P＜0.01 or ＜0.05),and UA had no significant correlation with FEV1％ (r =-0.092,P ＞0.05).Conclusions The serum UA and cytokines TNF-α,sICAM-1,IL-6,IL-8 are involved in the progression of COPD and correlated with lung function.They can be used as the judge indexes of COPD.

Objective To study the difference of cerebral infarction patients plasma oxidized low density lipoprotein,circulating endothelial cells,nitric oxide levels and normal people,and study the correlation detection index.Methods 50 patients with cerebral infarction were selected as the observation group,21 cases of normal people were selected as the control group.The oxidized low density lipoprotein,circulating endothelial cells,and nitric oxide were detected.Results The plasma oxidized low density lipoprotein,circulating endothelial cells,nitric oxide levels of the observation group were (687 ±169)μg/L,(9.0 ±1.7)cells/0.9 μL,(52.3 ±16.6)μmol/L respec-tively,which of the control group were (350 ±173)μg/L,(2.5 ±0.5)cells/0.9 μL,(77.4 ±21.1)μmol/L,there were statistifically significant differences between the two groups(t =5.278,8.982,4.371,all P <0.05).Correlation analysis showed that ox -LDL and NO(P =0.013,r =0.782),ox -LDL and CEC(P =0.019,r =0.793),CEC and NO(P =0.021,r =0.952),there were significant correlation.Conclusion Vascular endothelial injury degree will affect the plasma oxidized low density lipoprotein in patients with test results,so the clinical treatment should pay attention to the protection and antiinflammatory treatment on the function of endothelial cells.

Objective:To observe the effect of GSK-3? on the cell cycle and metastasis of human gastric carcinoma cell.Methods:Influencing the GSK-3? activity of human gastric carcinoma cell line SCG-7901 by LY294002 with or with not LiCl treatment.The expression of total GSK-3? and p-GSK-3?ser9 were detected in protein level by western blotting,cell proliferation and cell cycle distribution were tested by MTT and FCM respectively,the expression of E-cadherin and ?-catenin were measured with S-P immunohistochemistry and the cell-ground substance adhesion was tested by cell adherence assay.Results:The expression of total GSK-3? was constant and the protein level of p-GSK-3? ser9 was down regulated after exposured to LY294002 by 72 h,the cell proliferation was inhibited,cell cycle was arrest at G0/G1 phase,the E-cadherin level was positively regulated,the expression of ?-catenin and the adhesion rate were decreased.The effect on the proliferation、cell cycle、the expression of E-cadherin and ?-catenin and the adhesion by the LY294002 were partially abrogated by lithium chloride.Conclusion:GSK-3?could negatively regulate the proliferation of human gastric carcinoma cell by leading cells to G1-S arrest and inhibit its metastasis by positively regulating the expression of E-cadherin.

Objective:To study the deficiency ofshort stature homeobox containing (SHOX)gene deficiency ofshort stature and the relationship between genotypes and its corresponding phenotypes. Methods:Microsatellite analysis and direct sequencing were selected to analyze the deletions and mutations of SHOX gene in 203 cases of short stature;in addition ,anthropometric measures were assessed and radiographs of the left forearms and wrists in patients were collected to observe their phenotypes. Results:one mutation and eleven deletions were found;Detailed examination revealed certain dysmorphics,such as short forearm and lower leg,et al. Conclusion:In our study,the prevalence of SHOX deficiency in patients with short stature was 5.9% ,and certain correlations had been found between genotypes and corresponding phenotypes。

Abnormity of spirit, mind and intelligence are related to phlegm heat disturbing orifices. This article listed several diseases about phlegm heat disturbing orifices through three medical records, expounded the mechanism of applyingGuntan Pills, and proposed the matters needing attention to using the medicine, with a purpose to promote wider application ofGuntan Pills in clinic.

Objective To investigate the clinical characteristics ,diagnosis and prognosis of primary small intestine tumor . Methods Retrospective analysis was performed on the clinical data collected from the 47 cases of primary small intestine tumor . Results The clinical characteristics were non‐remarkable ,the preoperative diagnosis rate was 34 .0% (16/47) .Six cases (12 .8% ) were benign tumor ,adenoma was the main pathological type and the prognosis was good .Thirty‐three cases (70 .2% ) were malig‐nant tumor ,manifested mainly as anemia ,adenocarcinoma was the main pathological type ,originated from the duodenum mostly and the prognosis was poor .The 1 ,2 ,3 and 5 year survival rates of 26 cases in small intestine adenocarcinoma were 67 .4% ,42 .4% , 29 .4% and 22 .8% ,respectively ,and the median survival time was 20 .3 months .Eight cases(17 .0% ) were gastrointestinal stromal tumor ,most originated from the jejunum ,abdominal mass was the mainly clinical feature ,targeted drugs could help to improving the prognosis .Conclusion The incidence of primary small intestine tumor is low ,but most of them are malignant and the prognosis is poor .Find lesion in early stage with effective examinations and undergoing surgical resection are the key of treatment .

Objective To investigate the prevalence of thalassemia gene carried by child-bearing age people in Meizhou area. Methods Use regular blood tests and hemoglobin electrophoresis to screen the suspect thalassemia patients in couples of child-bearing age who underwent premarital examination or pregnancy check in the hospital.Samples with positive results were tested by using hybridization reverse dot blot to make a definite diagnosis of thalassemia.Results 208 samples were screened to be positive, among which there were 64 cases ofα-thalassemia carrier(30.67%),32 cases ofβ-thalassemia carrier(15.38%),3 case of combina-tion thalassanemia(1.92%).Conclusion The study may provide a reference for taking effective measures to prevent the birth of children with thalassemia.

GATA4 gene mutations are common in children with congenital heart disease.More than 120 mutations have been found in ventricular septal defect,atrial septal defects and the tetralogy of fallot.But the relationship between genotype and clinical phenotype has not yet been elucidated.This article summarizes the published germline and somatic mutations of GATA4 gene in human congenital heart disease,and provides insight into the phenotypic spectrum of GATA4 gene mutation.

Objective To detect the expression of insulin-like growth factor Ⅱ mRNA-binding protein 3 (IMP3) and p16 in cervical carcinoma and investigate their clinical significance.Methods Immunohistochemical Envision method was used to detect the expression of IMP3 and p16 proteins in 67 cases of carcinoma of the uterine cervix,58 cases of cervical intraepithelial neoplasia (CIN) and 15 cases of normal cervical epithelium.Results IMP3 was mainly dedected in the cytoplasm,whose positive expression rate was 85.07％ (57/67) in cervical carcinoma,12.07％ (7/58) in CIN (x2 =66.32,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =44.38,P ＜ 0.05).No significance variations of the expression of IMP3 were observed in cervical adenocarcinoma and squamous cell carcinoma (x2 =0.35,P ＞ 0.05).p16 was found both in nucleus and cytoplasm of cervical carcinoma,whose expression rate was 83.58％ (56/67),higher than 41.37％ (24/58) in CIN (x2 =24.85,P ＜ 0.05) and 0 (0/15) in cervical epithelium (x2 =42.61,P ＜ 0.05).Conclusion Expression of IMP3 and p16 is high in cervical carcinoma,maybe can be used as a potential biomarker of metastasis and progression.