Objective To study the level and clinical value of differential display code 3 (DD3) in peripheral blood of patients with prostate cancer.Methods 27 patients with prostate cancer from April 2014 to April 2015 in Affiliated Hospital of Hangzhou Normal University were researched.26 patients with non prostate diseases were selected as control group.DD3 mRNA levels were detected in peripheral blood of all patients.DD3 absorbance value of three groups of patients,the relationship between the relative content of DD3 mRNA and the clinical characteristics of prostate cancer,DD3 mRNA in urine and peripheral blood of patients with prostate cancer and benign prostatic hyperplasia were observed.Results In the patients with benign hyperplasia of prostate and non prostate diseases,a total of 4 patients did not appear to be DD3 specific bands.In prostate cancer patients,all patients were found to have DD3 specific bands.The relative content of patients with non prostate diseases was (0.18 ± 0.05) copies/mL.The relative content of benign prostatic hyperplasia patients was (0.30 ± 0.09) copies/mL.The relative content of prostate cancer patients was (0.78 ± 0.23) copies/mL.The positive expression rates of DD3 mRNA in peripheral blood and urine of patients with prostate cancer were 85.18％ (23/27),51.85％ (14/27),respectively,which were significantly higher than those in patients with benign prostatic hyperplasia[21.42％ (6/28),7.14％ (2/27)] (x2 =22.416,13.319,all P ＜ 0.05).Conclusion The specific expression of DD3 mRNA in peripheral blood of patients with prostate cancer can be used as an effective basis for judging the patients' condition,and it has certain value for the treatment and prognosis of patients with prostate cancer.

Objective To investigate the changes of microRNA-223 (miR-223) in patients with complication of sepsis after mini-invasive surgery of upper urinary tract stones, and to approach the value of miR-223 in the early diagnosis of sepsis. Methods Patients with upper urinary tract stones underwent micro-invasive treatment in the Department of Urology Surgery of the Affiliated Hospital of Hangzhou Normal University from January 2014 to January 2017 were enrolled. There were 60 patients with sepsis within 24 hours after surgery were assigned in the sepsis group, and 60 patients without sepsis were included in the non-sepsis group. The clinical data of miR-223, CD4+CD25+regulatory T cells (CD4+CD25+ Treg), procalcitonin (PCT) interleukin-10 (IL-10), tumor necrosis factor-α(TNF-α), C-reactive protein (CRP) in the blood, etc were collected within 24 hours after surgery. The differences in above indexes were compared between the two groups. The receiver operating characteristic curve (ROC curve) was drawn to evaluate the diagnostic values of blood mir-223, PCT and CRP in the patients, predicting whether septic complication after urinary calculi surgery would occur. Correlation analyses were used to analyze the correlations between mir-223 and CD4+CD25+ Treg, IL-10 and TNF-α. Results The miR-223 expression level and the contents of CD4+CD25+ Treg, IL-10, TNF-α, PCT and CRP in sepsis group were obviously higher than those in non-sepsis group [2ΔΔCt (×10-4):2.81±1.04 vs. 2.13±0.91, CD4+CD25+ Treg(×10-2): 17.61±4.48 vs. 8.37±2.71, IL-10 (ng/L): 58.42±16.38 vs. 34.68±12.45, TNF-α (pg/L): 249.41±30.69 vs. 167.54±25.98, PCT (ng/L): 4.45±1.89 vs. 0.31±0.08, CRP (μg/L):10.29±3.63 vs. 4.13±1.57, all P < 0.05); in sepsis group, the miR-223 expression and the level of CD4+CD25+ Treg (r = 0.367, P = 0.004) and IL-10 (r = 0.516, P = 0.006) were also significantly positively correlated, but miR-223 and TNF-α were not markedly correlated (r = 0.237, P > 0.05). The area under ROC curve (AUC), sensitivity, specificity and 95% confidence interval (95%CI) of miR-223 predicting sepsis occurrence after urinary operation were higher than those of CRP, PCT (AUC: 0.923 vs. 0.547, 0.769, the sensitivity: 81.73% vs. 71.23%, 66.59%, specificity: 86.00%vs. 42.00%, 83.00%, 95%CI: 0.862-0.979 vs. 0.351-0.679, 0.682-0.927). Conclusions The expression levels of plasma miR-223 in patients with sepsis after mini-invasive treatment for upper urinary tract lithotrity can reflect their immune reaction status, and can be one of the early diagnostic markers of whether the sepsis complication may occur after the surgery.

Objective To innovate the new training model of excellent nurse talents pool, so as to improve the quality of nursing professional team. Methods We established excellent nursing talents pool and set up 3 subsidiary pool including core specific personnel pool and nursing management backup talent pool and specialist pool. For different pool, we confirmed the targets of training, strictly selected the members, regulated selection procedure, made training plan, and scientifically checked and evaluated. The selected sample and managers were provided investigation about the attitudes of awareness and training demands by questionnaire. Results The nursing staffs presented a positive attitude for the establishment of nurse specialists pool, and the selected people hoped to be utilized multiple channel, multiple forms, and enhanced, supplied, improved the construction of knowledge, advanced nursing management, professional skills, teaching research, nursing development ability, and hoped the check and evaluation carried in clinical practice. To establish nursing specialist pool promoted connotation construction, and formed systematically the training model, and protruded the training target of practical nursing talents. Conclusions To establish excellent nursing talent pool cultivates the selected objects fully promoted all kinds of talents, innovates personnel training model; and provides the system guarantee for the correct selection and appointment of nursing talents.

Objective To explore the efficiency of training of professional nurses.Methods A total of 31 nurses were enrolled in a 12-week training of a comprehensive and systematic teaching practice containing theory teaching,skills training and clinical practice 3 domains between September and November in 2014.Nurses′nursing theory on specialized nursing,specialized nursing skills before and after the training were compared.Results After training,the accumulated score and item score of trainees on 50 knowledge points of burn specialized nursing theory, such as burn ward management system,monitoring skills on critically ill patients,perioperative skills,medication knowledge on specific treatment,explanation of common inspection and examination results increased compared to those before the training(P <0.05).Total and each item score of 3 skills operation assessment (body position change using turn-over bed,suspension bed application,cardiopulmonary resuscitation)of trainees after training were higher than those before training(P <0.05).Total score of self-growth evaluation and each item of 4 programs (nursing theory,basic nursing skills,intensive care level,nursing competence)of the trainees were higher than those before training(P <0.05).Conclusions Systematic and comprehensive theory teaching and clinical practice training can increase the nurses′level of professional theory and skills.Scientific and practical training content combined with training form close to clinic can effectively raise their comprehensive ability in nursing practice.