10.3969/j.issn.2095-4344.2013.25.022

Thelper 17 ( Th17 ) cells and regulatory T (Treg) cells are resistant to each other in their functions and differentiation pathways.Th17 cells and Treg cells play different roles in the development,treatment and prognosis of leukemia.Their functions are stronger and(or) numbers are more increasing than in the healthy controls.Th17 cells mainly function to promote inflammation and improve the body's defense capability to leukemic cells.Treg cells mainly mediate immune suppression and immune tolerance,and then inhibit the body's immune response to leukemia cells,which can results in poor treatment outcomes.Both Th17 cells and Treg cells have close relationships with the development,treatment and prognosis of leukemia.We would deal with the leukemia through the intervention of naive T cell differentiation.

Objective The purpose of this study was to investigate the effects of a breast cancer screening educational program on women's knowledge and intention to seek breast cancer screening in China.Methods 60 patients were divided into the experimental group (30 cases) and the control group (32 cases).The experimental group received breast cancer screening educational program,while the control group did not.A pre-test and post-test were used in both the experimental and the control groups.A convenience sampling was used.Two structured questionnaires were used.Results The mean scores of knowledge of breast cancer screening and intention to seek breast cancer screening of the experimental group were statistically different from those of the control group after the education.Statistical difference also existed in the experimental group in the above aspects within the experimental group.Conclusions Application of breast cancer screening educational program can improve women's knowledge and intention to seek breast cancer screening,as well as supply reference for prevention item of breast cancer.

BACKGROUND:Light-cured resin is a new kind of material with beautiful color, high strength, wide range of applications, easy-to-use and inexpensive features. Patients are wil ing to accept it because of less or no grinding of the teeth.
OBJECTIVE:To observe the effect of light-cured resin to fil the interdental space between maxil ary anterior teeth.
METHODS:A total of 32 patients aged 21-52 years were enrol ed, 107 teeth with 75 oversized maxil ary spaces, including 15 males and 17 females. One space between bilateral central incisors was found in 14 cases;three spaces between bilateral central incisors, left lateral incisor, and left canine were found in three cases;three spaces between bilateral central incisors, right lateral incisor, and right canine were found in two cases;four spaces between left central incisor, left lateral incisor, left canine, right central incisor, and right lateral incisor were found in three cases;three spaces between bilateral central incisors and lateral incisors were found in six cases;four spaces between left central incisor, left lateral incisor, right central incisor, right lateral incisor, and right canine were found in four cases. Al the oversized maxil ary spaces were fil ed with light-cured resin. Then, crown morphology, adaptation, color, degree of wear and loosening were observed during fol ow-up.
RESULTS AND CONCLUSION:After 3-year fol ow-up, 17 of 32 cases were successful in tooth repair:the crown morphology and tooth adaptation were good, and there was no prosthesis loosening or detachment and discoloration. The tooth space was repaired basical y in another nine cases:the crown morphology and tooth adaptation were not good enough, no prosthesis loosening or detachment was found, but there were some defects at the gingival margin and contact surface of teeth as wel as mild discoloration. Tooth repair failed in the other six cases with worse crown morphology and adaptation, prosthesis loosening or detachment, and severe discoloration, which need to be repaired again.

As a member of homeobox gene family,HOXA5 is expressed in many organs and can regulate gene expression,cell differentiation and morphogenesis.Its structure and (or) function abnormalities are closely related to the development of leukemia,breast cancer,brain hemangioma,hepatocellular carcinoma and so on.Researching the relationships between HOXA5 and tumors contributes to cancer diagnosis,treatment and prevention.

Six taxane diterpenoids were isolated from the stem bark of Tax us cuspidata Sieb. et Zucc.. On the basis of physical and chemical properties and spectra analysis,they were identified as 2'-de sacetoxyaustrospicatine(Ⅰ). taxol(Ⅱ),cephalomannine (Ⅲ), 2-deacetoxyl taxinine J (Ⅳ),taxinine B(Ⅴ) and 10-deacetyl-7-0-?-Dxylosyl-taxol (Ⅵ).

Objective To explore the effect of scalp acupuncture combined with rehabilitation on acute cerebral infarction patient's movement disturbance synchronously.Methods Fugl-Meyer Assessment was used to assess the motor function of scalp acupuncture combined with rehabilitation synchronously and non synchronously.Results The motor function of the group for scalp acupuncture combined with rehabilitation synchronously was improved prominently and was superior to control group on the 20th day and 30 day of the period of treatment.Conclusion The therapy of scalp acupuncture combined with rehabilitation synchronously and non synchronously can all improve the patient's motor function obviously,and the effect can increase with prolong of the period of treatment in a certain period. The advantage of the two Methods being used synchronously was appread and increased gradurally with prolong of the period of treatment and the therapy is worthy of being generalized.

Objective To determine impacts of p53 with R213 mutation on the expression of its downstream target gene p21 in HCT116-/-colorectal cancer cell line.Methods Mutations of p53 at R213Q (R/Q) and R213K (R/K) were respectively constructed and transfected into the p53 null HCT116 cells.The promoter activity drived by p53 was detected by dual-luciferase reporter system.EMSA assay was used to detect the ability of p53 binding to the promoter of its target gene p21.The expression of p53 downstream target genes p21 was examined by real-time PCR and Western blot experiments.Results The mutation of R213K disrupted the efficiency of p53 transactivation(P<0.05).The mutants were less efficient in targeting the consensus binding sequences of p53 in the regulatory region of p21 gene(P<0.05), In addition, The R/K mutant attenuated the expression of p21 gene(P<0.05).The mobility of R213Q was increased.Conclusions The different mutations of Arginine 213 have different impact on p21 in colorectal cancer cells.It could exist diverse regulating mechanisms.

Using RT-PCR, we can detect measles virus hemagglutinin gene (H gene) of 635bp length directly from measles vaccine strain Shanghai-191, Edmonston strain, nasopharyngeal aspirates and throat swabs of the measles patients. The primers of RT-PCR based on H gene sequence of measles virus did not give the same products from rubella virus and influenza viruses (A1, A3, B). By second PCR amplification, the minimum quantity of measles virus required to give a positive signal was<0.01TCID50. Since the IgM antibody positive rate in early stage of measles patients is always low, the specificity and sensitivity of RT-PCR method may help diagnosing measles cases.

Objective To analyze the clinical characteristic of disseminated intravascular coagulation(DIC)complicated by placental abruption,improving the diagnosis and treatment level.Methods A retrospective analysis of clinical data of placental abruption complicated by DIC was made in 28 cases.Results In 28 cases,hypertensive disorders in pregnancy( n =15) was the main cause,persistent abdominal pain and vaginal bleeding was the most common complaint.There were 6 cases delivery through vaginal,and 22 cases delivery by cesarean section,complicated by hemorrhagic shock in 9 cases,accounting for 32.1％,concurrent stillbirth in 20 cases,accounting for 71.4％.In 28cases,there were live births in 8 cases,after childbirth and newborn severe asphyxia in 6 cases,including 2 cases after the rescue of neonatal death,and another 4 patients after the rescue of neonatal survival.The occurrence of uterine subtotal or total hysterectomy was in 6 cases,accounting for 21.4％,acute renal failure in 2 cases,no maternal death.Conclusions Placental abruption complicated with DIC made a serious threat to maternal and child life and health,and the pregnancy should be terminated immediately after clinical diagnosis.DIC was not a hysterectomy indications,coagulation factors can help to correct the coagulation disorders,and to preserve the uterus and maintain the maternal physical and mental health.