AIM To investigate the effect of allicin on the regulation of VEGF mRNA expression in human hepatocellular carcinoma cells. METHODS Hepatocellular carcinoma cells were treated with the concentration 10 ?g?L -1 allicin in culture medium,and then the relative VEGF mRNA level at 8 h in human hepatocellular carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction using HPRT(hypoxanthine phosphoribosyltransferase)as an internal control standard. RESULTS The expression of VEGF gene mRNA was inhibited obviously by allicin. Compared with control group, the relative expression level of VEGF gene mRNA was decreased by about 66 36%( P

Objective To study the effects ofShenqi YizhiGranules (SQYZ) on learning and memory and content of Aβ1-42 of cerebral tissue in 5XFAD mice with Alzheimer’s disease; To discuss its mechanism on improving learning and memory ability of 5XFAD mice.Methods Four-month-old C57BL?6 wild type mice were randomly divided into NS control group and SQYZ control group, and the 5XFAD mice were randomly divided into model group, SQYZ group and huperzine-A (HupA) group, 15 mice in each group. Each group were given same volume for gavage for 60 d. After treatment, the learning and memory ability were evaluated by nesting test, passive avoidance and Morris water maze test. The senile plaques and content of Aβ1-42, ionized calcium binding adapter molecule 1 and glial fibrillary acidic protein in cerebral cortex and hippocampus were detected by immunohistochemical staining and immunofluorescence, respectively.Results Compared with NS control group, the score of nesting test in model group significantly decreased; the step-through latency in passive avoidance was shortened and the escape latentcy in Morris water maze test was prolonged; the quantity of senile plaques and content of Aβ1-42 increased in cerebral cortex and hippocampus; the activation of glial cells significantly increased. In the SQYZ group, the above-mentioned indexes reached or approached the level of wild type control mice. The difference between SQYZ group and model group was statistically significant (P<0.05,P<0.01).Conclusion SQYZ improved learning and memory ability in 5XFAD mice, which may be related to reduction of senile plaques, inhibition of over activation in glial cells and reduction of content of Aβ1-42 in cerebral cortex and hippocampus.

Objective To investigate the value of detection of 6 tumor markers:neuronspecific enolase (NSE),cytokeratin fragment 21-1 (CYFRA21-1),carbohydrate antigen 125 (CA125),carcinoembryonic antigen (CEA),Tumor Specific Growth Factor (TSGF) and squamous cell carcinoma antigen(SCC) in the clinical diagnosis of lung cancer.Methods From July 2013 to July 2014,174 blood samples were collected from 76 lung cancer patients,50 lung benign disease patients and 48 healthy controls.All the blood sample were detected for 6 tumor markers mentioned above by Immune chemiluminescence technique.Results Lung cancer patients have higher levels of 6 tumor markers than healthy controls(P ＜ 0.005) and have higher levels of 5 tumor markers except CA125 than patients with benign lung diseases(P ＜ 0.05).Among lung cancer patients,levels of 6 tumor markers were compared by 3 types of lung cancer as lung squamous cell carcinoma,lung adenocarcinoma and small cell carcinoma.Patients with lung squamous cell carcinoma have higher levels of SCC and CYFRA21-l than patients with other two types(P ＜ 0.05,P ＜0.01).Patients with lung adenocarcinoma have higher levels of CA125 and CEA than patients with other two types (both P ＜ 0.01).Patients with small cell carcinoma had the highest level of NSE(P ＜ 0.05).No significant differences of TSGF level were observed among the 3 types of lung cancer.Conclusion The combined detection of multiple tumor markers is very useful in the diagnosis of lung cancer and in the pathologic classification and better than single assay.