Objective To investigate the value of nerve conduction velocity detection in early diagnosis of peripheral neuropathy in type 2 diabetes.Methods Motor nerve conduction velocity (MCV)and sensory nerve con-duction velocity (SCV)for 94 patients were detected who was the first time diagnosed type 2 diabetes in our hospital from May 2014 to May 2015.The statistic results of nerve conduction velocity were conducted and then compared with clinical diagnosis to analyze the diagnosis rate for diabetic peripheral neuropathy (DPN).Results The abnormal rate of lower limb nerve conduction velocity was higher in the examined patients,which in the SCV of superficial peroneal nerve was especially highest(69.1%).The diagnostic rate of nerve conduction velocity in DPN was 71.3%,and the clinical diagnosis rate was 33.0%.The difference was statistically significant between the two diagnostic methods (χ2 =8.195,P =0.004).Conclusion Nerve conduction velocity detection has important value for the early diagno-sis of DPN.Patients for the first time diagnosed with type 2 diabetes should be carried out this detection to improve the diagnosis rate of DPN.

Objective To identify the effect of decreasing the rejection using the biological semipermeable membrane combined with the privileged site xenotransplantation. Methods The rat ICCs encapsulated by biological semipermeable membrane were xenotransplanted into dog's brain. The pathological changes of implants and surrounding cerebral tissues were observed under light and electric microscopy. The ?-cells of implants were identified by immunohistochemistry. Results After 2 months of transplantation, The ?-cells were observed and the lymphocytes were dispersed in the grafts.The brain tissues near the grafts showed slight edema and glial hyperplasia. Conclusion The method using the biological semipermeable membrane in combination with the privileged site xenotransplantation have a beneficial effect on the inhibition of the rejection of heterogeneous ICCs implant.

Objective To explore effects of doctor-nurse integrated care management model on fast track (FT) perioperative management of colorectal cancer patients.Methods Between January 2014 and September 2014,112 colorectal cancer patients (innovation group) underwent doctor-nurse integrated care and FT management in West China Hospital were analyzed retrospectively;who were compared with 96 patients (control group) underwent FT management from the same period of 2011.Early postoperative rehabilitation indicators,length of hospital stay,30d readmission rates,total hospitalization costs and patients satisfaction were compared.Results Innovation group of patients for the first time of post-operative flatus,defecation,oral feeding,ambulation,gastric and drainage tubes removal time were all earlier than those in control group,P ＜0.05,while the difference of urinary catheter tube removal time between the two groups was not statistically significant,P ＞ 0.05;the length of hospital stay in innovation group(6.05±2.05) d was significantly shorter than that in control group (7.85±3.22) d,P ＜ 0.05;preoperative hospital stay in innovation group was (1.35±0.45) d,shorter than that in control group (2.56±0.63)d,P ＜ 0.05;the patients satisfaction points in innovation group was (94.t2±3.12) points,which was higher than that in control group (86.36±5.51) points,t=2.589,P ＜0.05.Conclusion Doctor-nurse integrated care management model can improve postoperative rehabilitation effects,can reduce average hospital stay,total hospitalization costs and improve patients satisfaction.

Objective: To explore the relationship between interpersonal forgiveness and revenge and some influential factors in college students. Methods: Rumination Scale,Empathy Scale, Tendency to Forgive Scale and Revenge Motivation Scale were administered to 194 college students. Results and Conclusion: ① There were significant gender differences in the degree of seeking revenge and rumination. ② Most of interpersonal transgression indeed happened between fellow students. ③ Correlation analysis showed significantly negative correlation between forgiving and hurtfulness, revenge motivations, rumination, and between revenging and tendency to forgive, empathy; and positive correlation between forgiving and empathy and between revenging and revenge motivation, hurtfulness. Further regression analysis revealed that revenge motivations, rumination and empathy were significant negative predictors of forgiving; hurtfulness and revenge motivations were significant positive predictors of revenging.

Objective To investigate a new method of a long term culture for rat islets in vitro. Methods Rat islets marked by green fluorescent protein (GFP) were cocultured with Sertoli cell for 20 weeks. Histological studies were performed on islet group and coculture group in 1w, 3w, 10w, 15w, 20w of culture by light, fluorescent and electron microscopy. Insulin released was measured by radioimmunoassay. Results In islet group, islet viability and the number of insulin positive cells were significantly decreased after 3w of culture, cumulative quantities of insulin for 24 hours and the stimulation index also fell rapidly under this condition, meanwhile the ultrastructure of islets was destroyed. However, under coculture condition, culture time of islets was prolonged in vitro, islet viability and the number of insulin positive cells were significantly increased, cumulative quantities of insulin for 24 hours and the stimulation index maintained at high level, and the ultrastructure of islets remained normal even after 20w of culture. Conclusion Coculture of rat islets with Sertoli cells may promote islet growth and prolong culture time, and it is a new method of a long term culture of islets in vitro.

Objective To explore the characteristics of the gene expression in Cushing′s adrenal corticoadenoma and its associated molecular mechanism. Methods cDNA libraries of human normal adrenal and the adenoma were constructed. Large scale sequencing and bioinformatics were used. Results From the tumor library, 2089 expressed sequence tags (ESTs) were obtained with a successful rate of 98.9%. The functional distribution of the known genes in the tumor coincided with that in normal adrenal. Some synthases such as 3bHSDⅡ and P450c17A involved in steroidogenesis were expressed more highly in the adenoma than those in normal adrenal, especially 3bHSDⅡ. By comparison with the expression abundance of genes and statistic analysis, the differences were significant in 45 genes between the tumor and normal adrenal libraries. Interestingly, some genes associated with tumorigenesis, including heat shock protein 90, metallopanstimulin (MPS1), adenine nucleotide translocators etc, were highly expressed in the adenoma. However, those genes related to apoptosis and suppressing proliferation were not found in the tumor library. Conclusion The adenoma shows exuberant activities in steroid synthesis. Not only does the tumor highly express the genes associated with tumorigenesis, but also those related to apoptosis and suppressing proliferation lowly existed in the tumor.

Objective To evaluate the application of mobile ultrasonoscope in earthquake rescue. Methods According to the characteristic of environment and traumatic conditions in earthquake area, the mobile ultrasonoscope was used to diagnose for clinicians, and the advantages and disadvantages were evaluated. Results The mobile ultrasonoscope played an important role in routine diagnosis, such as organic injury and calculus, superficial organellae injury, soft tissue injury, injury of limbs and blood vessels, gynaecology and obstetrics and so on. As far as certain conditions were conferred in which the mobile ultrasonoscope was seldom used to diagnose in common practice, it played a dramatic role too in bone fracture, joint damage, craniocerebral injury and foreign objects. Conclusion In routine diagnosis or certain conditions, the mobile ultrasonoscope is very practical, especially in earthquake rescue.

Objective To investigate the significance of MMP-9 expression in the pathogenesis of hepatic encephalopathy.Methods 180 rats were randomly divided into the control group,hepatic encephalopathy 4,8 and 12h groups,45 rats in each group.In hepatic encephalopathy group,rats were intraperitoneally injected D-galactosamine (400mg/kg) with lipopolysaccharide (100μg/kg),and then the symptom was observed.Ammonia,the evans blue(EB) content,the brain water content and the MMP-9 mRNA in the brain tissue were assessed by real-time Q-PCR after 4,8 and 12h.Results 2h later the rats in hepatic encephalopathy groups began to appear the symptom.4 hours later,ammonia,the EB content,the brain water content and MMP-9 mRNA content of the hepatic encephalopathy groups began to increase [(74.27 ± 1.77) μ mol/L vs (33.85 ± 1.50) μ mol/L,F =5 983.36 ;(4.84 ±0.43)μg/g vs (3.96 ±0.48)μg/g,F =111.54; (78.63 ± 0.46)％ vs (76.07 ±0.39)％,F =737.25,and (1.037 ± 0.071) vs (0.829 ± 0.053),F =132.10,P ＜ 0.01],and with the extension of time increased obviously [(136.32 ± 3.88) μmol/L and (279.53 ± 9.78) μmol/L,F =5 983.36 ; (5.94 ± 0.35) μg/g and (6.71 ±0.50)μg/g,F=111.54;(80.50±0.32)％ and (82.14±0.30)％,F=737.25;(1.235 ±0.095) and (1.425± 0.113),F =132.10,P ＜ 0.01].Conclusion Blood brain barrier permeability enhancement and brain edema in hepatic encephalopathy rats may be related with MMP-9 mRNA increasing.

Objective: To investigate the prevalence and influencing factors of latent tuberculosis infection (LTBI) among freshmen in a vocational college in Xinjiang Uygur Autonomous Region, so as to provide insights into tuberculosis control in vocational colleges. Methods : All freshmen enrolled in a vocational college in Xinjiang Uygur Autonomous Region in 2020 were recruited and participants' demographics, life style, history of tuberculosis contacts, frequency of ventilation and disinfection in dormitories and classrooms and awareness of core tuberculosis control knowledge were collected through questionnaire surveys. LTBI was detected among freshmen by means of purified protein derivative test (PPD skin test) and chest X-ray scans, and the factors affecting LTBI were identified using a multivariable logistic regression model. Results: A total of 5 463 freshmen were screened for LTBI, with a screening rate of 100.00%, and the participants included 2 151 men and 3 312 women, with a male/female ratio of 0.65︰1, and had a mean age of (16.83±1.05) years. A total of 388 freshmen were detected with LTBI, with a detection rate of 7.10%, and no active pulmonary tuberculosis was detected. The prevalence of LTBI was 9.44% in men and 5.59% in women. Multivariable logistic regression analysis identified males (OR=1.587, 95%CI: 1.281-1.965), smoking (OR=2.108, 95%CI: 1.355-3.278) and a history of tuberculosis of families (OR=2.851, 95%CI: 1.615-5.034) as risk factors for LTBI among freshmen, and knowing core tuberculosis control knowledge as a protective factor of LTBI among freshmen (OR=0.276, 95%CI: 0.218-0.350). Conclusions The risk of LTBI was associated with gender, smoking, history of tuberculosis of families and understanding of the core tuberculosis control knowledge among freshmen in this vocational college in Xinjiang Uygur Autonomous Region. Health education about the core tuberculosis control knowledge is recommended to be reinforced among students in vocational colleges in Xinjiang Uygur Autonomous Region.

Objective To evaluate the effects of body position and cuffed endotracheal tube (cETT) position on the intracuff pressure during tonsillectomy in the pediatric patients.Methods Sixty pediatric patients,aged 3-12 yr,scheduled for elective tonsillectomy under general anesthesia,were divided into group A and group B using a random number table,with 30 patients in each group.The cETTs were placed on one side of the blade of Davis retractor in group A.The cETTs were placed between the blade of Davis retractor and the body of tongue in group B.The intracuff pressure was recorded when the head was in the neutral position (T1),after changing to the head back position (T2) and when the retractor was placed for operation (T3).Results The intracuff pressure was significantly higher at T2 than at T1 in the two groups (P＜0.01).The intracuff pressure was significantly higher at T3 in group A and lower at T3 in group B than at T2 (P＜0.01).Compared with group A,the intracuff pressure was significantly decreased at T3 (P＜0.01),and no significant change was found in the intracuff pressure at T1,2 in group B (P＞0.05).Conclusion The intracuff pressure is affected by body position and cETT position during tonsillectomy in the pediatric patients,and routine monitoring of the intracuff pressure is recommended.