OBJECTIVE:To provide reference for the effective integration of standardization strategy of traditional Chinese medicine (TCM) and intellectual property. METHODS:Through analyzing the conflict between standardization requirement of TCM and intellectual property system,the coordination between TCM standards and intellectual property especially the trade secret rights was explored. RESULTS:The information disclosure of TCM standards and intellectual property strategy especially protec-tion of TCM trade secrets faced confrontation and conflict in system and design. For the suggestions to solve it,one is to reconfirm the role and positioning of the government on the adaption of standardization in public law field;another is to establish coordina-tion principle of information disclosure and confidentiality in private law field,and achieve difference open and moderate open of information. CONCLUSIONS:The TCM standardization is not absolute,the pros and cons is the precondition to solve the conflict between the standardization strategy and trade secret system in TCM in terms of protection of information freedom and secret infor-mation.

Objective To established the method of increasihg the proportion of T regulatory cells(Tr) to T effector cells(Te), which could suppresses allogeneic ahtigen reaction, by in vivo of glucocorti-coid (dexamethasone, DXM) combined with IL-2. Methods After combined treatment to male C57BL/6N mice (donor) with DXM(5 mg. kg-1· d-1 ) and IL-2 (300 000 IU · mouse-1·d-1) for 3 d, spleen mono-nuclear cells were made and were carried out by flow cytometry analysis. Using the spleen cells of BALB/c mice as aliogeneic antigen to stimulate the spleen cells of male C57BL/6N mice for 7 d after combined treat-ment of glucoeorticoid and IL-2, the reaction of cell proliferation was detected. Results After the treatrment of DXM and IL-2, CD4+ CD25+ Foxp3+ Tr cells in the spleen of C57BL/6N mice increased abviously. The ratio of CD25+ Foxp3+ Tr to CD4+ T cells was 24.22%±7.60% in the group of DXM combined with IL-2, while the control group was 4.02% ±0. 84% ( P =0. 01 ). Compared with the control group (0. 14±0.01 ), the ratio of Tr to Te increased obviously in the group of DXM combined with IL-2 (0.43±0. 15 ) ( P = 0.01 ). The group of DXM combined with IL-2 also expressed more glucocorticoid-induced tumor necrosis factor receptor(GITR) and alloreaction was suppressed/n vitro obviously ( P < 0. 05 ). Conclusion DXM amplifies IL-2 induced the proportion of Tr to Te, and suppresses the cell proliferation stimulated by alloge-neic antigen.

Objective To explore the use of enzyme-linked immunosorbent assay(ELISA) to test the serological BP180NC16A-ELISA index in patients with bullous pemphigoid (BP) and investigate the relationship of this index with disease monitoring and therapeutic guidance.Methods The serological BP180NC16a-ELISA index at different times was examined in twenty BP patients with ELISA kit and monitor changes of pre-treatment BP180NC16a-ELISA index condition score and efficacy relationship..Results Twenty patients tested by ELISA had 19 cases of positive patients with positive rate of 95.0％ and one case negative with negative rate of 5.0％.After treatment,19 cases (19.60 ±4.63)d became stable,and condition score was (3.75 ± 1.37) points.After continued treatment (28.75 ± 6.11) d entering clinical remission,condition score was (2.25 ± 1.21)points.Compared with the score before treatment condition [(5.55 ± 1.61) points],differences were statistically significant (Z =-3.808,-3.965,P ＜ 0.01),and clinical remission score below clinically stable condition (Z =-3.808,P ＜0.01).A total of 19 cases of patients' pre-ELISA treatment index were positive correlation with after treatment stable period and clinical remission period (rs =0.788,0.752,P ＜ 0.01).Conclusions This study demonstrated that ELISA-detected BP180NCI6a-ELISA index was positively correlated with the BP disease severity and clinical course,which could be as the guideline to monitor and clinical treatment.

Objective: To analyze the prevalence of reduced visual acuity (VA) and to examine the association between reduced visual acuity and influencing factors among Chinese Han students, so as to provide bases for the prevention and treatment of reduced visual acuity.Methods: Subjects were from 2014 Chinese National Surveys on Students' Constitution and Health (CNSSCH).In this survey, 261 832 Han students aged 7-22 years participated.Unaided distance VA was measured using a retro-illuminated lgMAR chart with tumbling-E optotypes.Ametropia was determined by using serial bronchoscopy.Logistic regression was used to assess the association between reduced visual acuity and influencing factors.ArcMap 10 was used to map the regional distribution of reduced visual acuity.Results: Nationwide, the overall prevalence of reduced visual acuity was 66.6%, and was highest among the urban girls (73.5%).The prevalence of myopia was 60.8% and accounted for more than 90% among the students with reduced visual acuity.Compared with 2010 CNSSCH, the increments of reduced visual acuity were highest in the students aged 13-15 years (6.8 percentage points).The prevalence of reduced visual acuity ranged from 50.6% to 76.2% in each province (autonomous region, municipality) in 2014 CNSSCH.The top three of the prevalence of reduced visual acuity were Jiangsu (76.2%), Zhejiang (76.0%) and Shandong (75.9%), and the lowest three were Hainan (50.6%), Guizhou (53.9%) and Xinjiang (57.6%).Logistic regression showed that the girls, urban students, and students who spent ≥2 h per day in their after school homework were more likely to develop reduced visual acuity, but the students with physical activity time ≥2 h per day were less likely to develop reduced visual acuity.Conclusion: Although the situation was different in each province (autonomous region, municipality), reduced visual acuity has become a public concern affecting the health of students in China.Based on the intervention on the key populations, such as urban girls and pre adolescent students, we should also continue to strengthen the prevention and control for reduced visual acuity among all students.

Objective To investigate the effect of moderate pain on attentional bias towards emotional pictures among healthy subjects.Methods Thirty-two healthy college students aged from 17 to 26 (21.8±2.2;16 males and 16 females) participated in this study.A tourniquet was tied to each subject's left upper arm 1 to 2cm above the cubits horizontal grain.Pain was inflicted by inflating the tourniquet,and the pressure was maintained at 26.66kPa.While tourniquet was inflated (with pain) or not (no pain),each subject was asked to finish a pictorial dot-probe task with three kinds of pictures-emotionally positive,negative and neutral.In experiment 1,subjects performed the dot-probe tasks with the contralateral hand while the tourniquet was tied on the left upper arm without inflation.In experiment 2 the tourniquet was inflated until the subject completed the dot-probe task (for about 10min).The reaction times (RTs) and the error rates (Ers) in the recognition task were recorded,and the intensity of the subject's pain and discomfort were measured using a verbal rating scale.Results The subjects reported moderate to severe pain with the tourniquet inflated.The RT and ER data were analyzed using two-way analysis of variance (ANOVA) which showed a significant difference between the average RTs of the males (482±73ms without pain and 466±82ms with pain) and those of the females (536±90ms without pain and 519±100 ms with pain).The average ER was significantly different between the pain (2.38±1.49)％ and no pain (1.09±0.82)％ conditions in both groups.Holn-Sidak multiple comparison testing showed significant differences in both groups' average ER between the negative picture (3.81±1.73)％ and the positive picture (1.66±0.97)％,and between the negative and neutral pictures (1.68±0.8) ％ in the pain condition.Mild attentional avoidance was observed with the positive [pain condition (-5.1±4.8) ms and no pain (-4.6±4)ms] and negative pictures [pain condition (-3.43±6) ms and no pain (-0.79±4.1)ms],but no significant difference was found between the pain and no pain conditions.Conclusion The error rate in a pictorial dot-probe task is influenced by pain,especially with negative pictures.

Objective To investigate correlation of right ventricular hypertrophy and vitamin D , atrial fibrillation and NT-proBNP in patients with pulmonary heart disease.Methods 40 cases of pulmonary heart disease were selected, according to whether the right ventricular hypertrophy or not, and divided into hypertrophy group and non hypertrophy group.Echocardiography, serum 25 hydroxy vitamin D, 24 h dynamic electrocardiogram and NT-proBNP level were detected, and the correlation were detected between the right ventricular hypertrophy and influence factors.ResuIts The right ventricular anterior wall ( RVAW) and right ventricular diastolic diameter ( RVDd) in hypertrophy group were significantly greater than those in non hypertrophy group (P<0.05).The serum 25 hydroxy vitamin D levels in hypertrophy group was lower than that in non hypertrophy group (P<0.05), but there was no significant correlation of right ventricular hypertrophy and 25 hydroxy vitamin D levels(r=-0.189,P=0.424).The thickness of right ventricular anterior wall in atrial fibrillation group was greater than that in non atrial fibrillation group (P<0.05), but there was no significant correlation of right ventricular hypertrophy and atrial fibrillation times(r=0.178,P=0.452).Serum NT-proBNP levels in hypertrophy group was higher than that in non hypertrophy group (P<0.05), but there was no significant correlation of right ventricular hypertrophy and serum NT-proBNP levels(r=-0.105, P=0.660).ConcIusion Patients with right ventricular hypertrophy of pulmonary heart disease who has a lower serum 25 hydroxy vitamin D level and a higher NT-proBNP level.Patients with atrial fibrillation has a thicker RVAW.However, there was no correlation of above influence factors.

Objective This research aimed to investigate the function mechanism of lung injury after acute pulmonary embolism.Methods 30 healthy New Zealand rabbits were selected and randomly divided into five groups:control group (with venepuncture applied) and model groups including none-saline (NS) control group,exogenos laptin low doses group,medium dose group and high dose group.ELISA was applied to examine the TNF-αand IL-1β in serum.Results TNF-α and IL-1β content in saline group were higher than that in control group,and the results were statistically significant.In medium dose (50 μg/kg) group,TNF-α and IL-1β levels decreased apparently and the results were statistically significant.In low doses (20 μg/kg) group,TNF-α and IL-1β levels decreased,but the results were not statistically significant.In high dose (100 μg/kg) group,TNF-α and IL-1β levels increased on the contrary.Conclusion Suitable dose of leptin will lead the decrease of postoperative levels of TNF-α and IL-1β after acute pulmonary embolism,which thus have protection function to the injured lung.

Objective To learn the levels of serum 25-(OH)D in 0 12 years old chindren in Kunming.Methods Serum 25-(OH)D levels of children (4 498) were measured by using Roche cobas 8 000 / E602 automatic electrochemical luminescence immunoassay.Results As the increase of age,the levels of serum 25-(OH)D were gradually decreased,different age groups had significant difference (P＜0.05).The levels of 25-(OH)D between boys and girls had no significant difference (P＞0.05);The average level of sernm 25-(OH)D was 83.48 nmol/L,the level of serum 25-(OH)D was distributed in 0 ～ 1 years old group,and most concentrated in the 6 ～ 9 years old group.Conclusion The levels of 25-(OH)D in 0 ～ 12 years old chindren are in a good status,but with the increase of age,the level of 25-(OH)D is decreasing gradually,especially the levels of 25-(OH)D decreased significantly after the age of three,in shortage or lack of status,this should cause everybody's attention.

Objective To observe the leading effect of end-tidal pressure of carbon dioxide in artery ( PET CO2 ) on mechanical ventila-tion in New Zealand white rabbits, and to establish parameters for medical animal experiments in terms of hemodynamics, blood gas, blood glucose, electrolyte. Methods 31 anesthetized New Zealand rabbits were practiced tracheostomy tube and mechanical ventilation. Respira-tion rate was 40 breaths/min and tidal volume was adjusted so that PET CO2 was 29 mmHg. Invasive blood pressure, electrocardiogram and PET CO2 were monitored. Blood gas analysis, electrolyte, hemoglobin and blood glucose were tested. Results When PET CO2 was maintained at 29 mmHg, the results were as follows:PH (7.42 ±0.07), 95% confidenceinterval (7.40~7.45);PaCO2(38.5 ±5.8) mmHg, 95%confidenceinterval (36. 4~40. 6) mmHg;BE (1. 45 ± 2. 80) mmol/L,95% confidenceinterval (0. 43~2. 48) mmHg. Conclusion Moni-toring of PET CO2 is good to guide mechanical ventilation in New Zealand white rabbits.

Objective To evaluate the efficacy of laparoscopic operation combined with GnRh-a in treatment of en-dometriosis. Methods Selected 36 cases of laparoscopic operation in treatment of EMS patients as control group from January 2012 to January 2013 in our hospital, another seleceted 36 cases of EMS patients at the same period used GnRh-a combined laparoscopic operation treatment as a united group,after the treatment,followed up for 12 months,the recurrence rate,pregnancy rate were compared between two groups. Results The total effective rate of treatment group was 88.9%,significantly than the control group,the difference was significant (χ2=4.397,P<0.05). In 4 cases of recurrence within 1 years after discontinuation of the control group(1 cases in stageⅣ,3 cases in stageII),the recur-rence rate was 11.1%(4/36),manifested as dysmenorrhea or pelvic mass. A control group of 16 cases of infertility pa-tients 2 cases of pregnancy,the pregnancy rate was 12.5%, without the occurrence of ectopic pregnancy. Recurrence within 1 years after stopping drug combined group 1 cases (Ⅳ),the recurrence rate was 2.8%(1/36),the 18 infertile patients 8 cases of pregnancy,the pregnancy rate was 44.4%. the recurrence rate of combined group was lower than the control group,the difference was significant(χ2=7.354,P<0.05),the pregnancy rate in combined group was significantly higher than control group,the difference was significant(χ2=6.327,P<0.05). Conclusion The curative effect of GnRh-a combined with laparoscopic operation in the treatment of endometriosis is accurate,can reduce the recurrence rate,im-prove the pregnancy rate,worthy of promotion and application.