Objective To compare the number of endothelial progenitor cells (EPCs) from peripheral blood in patients with acute myocardial infarction of young man and healthy man.Methods Eighteen young men (18 ～50 years old) with acute myocardial infarction (AMI) who were admitted at the First Affiliated Hospital of Dalian Medical University from June 2010 to April 2011 in young man were enrolled,aged (65 ～ 85 years old) men with acute myocardial infarction in 18 cases,within 24 hours of onset collected blood 2 ml.Ten cases of healthy young men (30 ～50 years old) were used as control group,fasting venous blood 2 ml.A volume (400 μl) of blood was taken to red blood cell lysis buffer hemolysis labeled with vascular endothelial growth factor (VEGF),CD34,and CD133 antibodies,and then analyzed with flow cytometry.Results The number of EPCs in peripheral blood was measured in young male AMI group.The number of EPCs in peripheral blood was (0.58 ±0.83)％ in older men.The number of EPCs in peripheral blood of AMI group was (0.04 ± 0.03) ％.For healthy controls,the number of EPCs was (0.02 ± 0.02)％.The number of EPCs was significantly higher in AMI patients compared to control group (P ＜ 0.05).However,for AMI group,the increased number of EPCs in young men was significantly greater than young female (P ＜0.01).Conclusions The number of EPCs in peripheral blood in young man AMI patients is significantly increased within 24 hours.

0.05). A relatively low negative correlation was found between P~(2+) content in outer layer of dentin and dfs (r=-0.415 96, P=0.043 2). Conclusions:P~(2+) content in different part of deciduous teeth is different and it is not related to caries status.

Objective To compare the muscular relaxation effect and side effects of Cisatracuri-um and Succinylcholine for children on tracheal foreign body removal.Methods Forty ASA Ⅰ pediat-ric patients,undergoing electivetracheal foreign body removal,were randomly divided into two groups:group C,received cisatracurium 0.2 mg/kg for induction,0.05 mg/kg for maintenance,n=20;group S,received succinycholine 2 mg/kg for induction,0.4 mg/kg for maintenance,n=20.All patients were performed with controlled ventilation assisted by high-frequency jet ventilation (HFJV).We recorded bronchoscope condition,muscle relaxant supplements,extubation time and the incidence of muscle soreness in postoperative 24 h.Results There was no significant difference in bronchoscope condition between these two groups.The extubation time were significantly longer in group C than in group S (P<0.05).The incidence of muscle soreness in postoperative 24 h were sig-nificantly higher after the operation in group S than that in group C (P<0.01).All patients in group S needed muscle relaxant supplements,but none in group C (P <0.01).Conclusion Both Cisatra-curium and succinylcholine can provide good muscle relaxation effect on tracheal foreign body remov-al,but cisatracurium prolong the extubation time,and succinylcholine increase the incidence of muscle soreness.

Objective:To investigate the changes of gastric tissue COX2 after intestinal perforations due to abdominal firearm wound.And to study its relationship with plasma endotoxin levels and pathological change of gastric tissue.Methods:A total of 42 Chang-Bai piglets were assigned randomly into 7 groups:control group and wounded 1,2,4,8,12,24 hours group.The model of intestinal perforations due to abdominal firearm wound was established in wounded groups.Gastric tissue COX2 activity was measured with immunohisto-chemical staining and image analysis in all groups.The plasma endotoxin levels were measured by chromogenic limulus amebocyte lysate test.The alterations of gastric tissue were observed under light microscope in all groups.Results:The expressions of COX2 of gastric tissue in wounded groups were significantly increased compared with control group (P

Objective To study the clinical effect of sevoflurane combined with remifentanil in laparoscopic cholecystectomy (LC).Methods50 cases of laparoscopic cholecystectomy in Tianjin Haihe Hospital from February 2015 to September 2016 were selected as the research object, and randomly divided into the control group and the experimental group, each group had a total of 25 cases.The patients in control group were treated with continuous intravenous infusion of propofol.The experimental group received sevoflurane.Patients in the experimental group and the control group were given remifentanil after surgery.Compared with the experimental group and control group of patients with the degree of recovery and related Linchuan indicators.ResultsAfter the corresponding anesthesia, the control group of patients with recovery time was (13.4±3.1) minutes, the score was (4.1±1.2) points, extubation time was (14.6±3.7) minutes.However, the extubation time and recovery time of the control group were significantly longer than those of the experimental group, and the recovery score was lower than that of the experimental group, with statistical difference (P<0.05).There was no obvious adverse reaction in the experimental group and the control group, and there was no significant difference in the changes of cardiovascular factors.ConclusionSevoflurane combined with remifentanil for laparoscopic cholecystectomy can improve the anesthetic effect to a great extent, stable operation and postoperative patients with cardiovascular function, recovery time is short, the clinical significance of only one step promotion and application.

Purpose　To investigate relationship between androgen receptor and apoptotic index and to further understand the tumor biology of prostate cancer. Methods　Fifty-six patients with histologically proven prostate cancer and 20 cases with benign prostatic hyperplasia(BPH) were collected. Androgen receptor(AR) were stained by 2H12 monoclonal antibody using immunohistochemical method. The apoptotic index (AI) was determined by the terminal deoxynucleotidyl transterase-mediated dUTP biotin nick end labeling (TUNEL) technique on serial sections of formalin fixed, paraffin embedded tissues. Results　AR was not found significantly difference (P>0.05) between prostate cancer and BPH. The association of AR with AI according to Gleason score were not observed in prostate cancer. AI were significantly higher in prostate cancer compared to BPH (P<0.05).AI were also significantly higher in AR-positive prostate cancer than in AR-negative prostate cancer（P<0.05）. Conclusion　AR expression can induce prostate cancer cells to become apoptosis, and may be a useful predictor in functional classification and endocrine response of prostate cancer.

0.05). (2) There was a significant difference in the distribution frequency of ?_2-AR at locus +46 between mild, moderate and severe degrees of PIH (P

Objective: To investigate the survival status and prognostic factors of patients with leptomeningeal metastasis (LM) from lung adenocarcinoma. Methods: The survival rate and prognostic factors of 65 patients with LM from lung adenocarcinoma, who had complete follow-up data, were retrospectively analyzed. Results: The median survival time of the 65 patients was 7.4 months,and the 1-year survival rate was 6.2% (4/65). Univariate analysis demonstrated that gender, age, smoking history, timing of LM and whether in combination with brain metastasis had no significant correlations with overall survival (all P > 0.05); while the Eastern Cooperative Oncology Group (ECOG) performance status (PS) score, ventriculo-peritoneal (V-P) shunt, epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) targeted therapy, systemic chemotherapy (SC), whole-brain radiotherapy (WBRT), clinical signs of LM and EGFR gene status were associated with the overall survival (all P < 0.05). Multivariate analysis showed that EGFR gene status, ECOG PS score, SC and V-P shunt were independent prognostic factors of the prognosis of patients with LM from lung adenocarcinoma (all P < 0.05). Conclusion: The overall prognosis of patients with LM from lung adenocarcinoma is poor. The prognosis of patients with LM bearing EGFR mutation is relatively good. EGFR-TKI targeted therapy, SC and V-P shunt can prolong the survival time and improve the prognosis of patients with LM metastasis from lung adenocarcinoma.

Objective To measure the diameter of cochlear nerve(CN) in normal hearing children with different ages,and evaluate the diameter variations with age.Methods A total of 156 normal-hearing children were assessed with 3D-FIESTA sequence scanning of the inner ear.All the subjects were divided into 13 groups according to age,witha group of ≤6 months,a group of ≤1 year,and 11 groups from one year to 12 years.Each group had 12 cases,including 6 boys and 6 girls.The diameter of CN was measured at the entrance of the cochlea,the middle of internal auditory canal(IAC) and the fundus of IAC respectively on the axial and oblique sagittal images of 3.0 T MRI by two independent observers.The average values measured by them were the final results.The intraclass correlation coefficient was used to determine the consistency between the two independent observers.The t test was used to access differencesin CN diameter by sex and sides.One-way analysis of variance(ANOVA) and two-way ANOVA were used for comparisons among different groups.Spearman correlation coefficient was applied to find a correlation between the CN diameter and age.Results The diameters of normal-hearing children's CN at the middle of the IAC,IAC fundus and the entrance of the cochlea were (1.12 ± 0.08),(1.05 ± 0.06),(0.87 ± 0.14) mm respectively,and there was significant difference among the three measuring points (F=527.57,P＜0.05).The diameters of the CN had no significant differenc (P＞0.05) in age-groups,gender and sides(P＞0.05),and there was no correlation between the diameters of normal children's CN and age.Conclusions The diameters of normal-hearing children's CN change with different points of the IAC,of which the maximum value is at the middle of the IAC,followed by the IAC fundus,and the entrance of the cochlea is at the minimum,more over the normal size doesn't change with age.

Objective To determine the effects of non-thermal biological effect of low frequency pulsed electromagnetic field (LFPEF) with 15 Hz and 30 mT on ulcer wound during the plateau stress ulcer cure.Methods Thirty male Sprague-Dawley rats were assigned to three equal groups:control,normal cure group (NC) and exposure treatment groups (ET).The plateau rats stress ulcer models were constructed with the combination method of a hypoxic and low-pressure acclimation and the stimulation of absolute ethyl alcohol irrigating stomach.The LFPEF equipment generated the LFPEF of 15 Hz,40％ duty cycle and 30 mT to expose and treat the rats' stomach of ET group with the frequency of 3 hours each day.After 6 days of exposure treatment,the stress ulcer cure situation of each group was evaluated by the methods of macroscopic observation,the gastric juice pH values detection and ulcer index (UI) measurement.Results After 6 days of model,the macroscopic observation showed that the ulcer of NC group was worse than the that of ET group (P＜0.05).The pH values and UI of ET group were significantly different with those of NC group,and closed to those of the control group.Conclusion The nonthermal biological effect of LFPEF could promote the plateau stress ulcer cure.