Objective To determine the impact of baccalaureate nursing students' psychological capital on core competency.Methods A total of 280 baccalaureate nursing students from a certain third-level and grade-A hospital in Tianjin were tested by the Chinese version CINS Scale and Chinese version of PsyCap Scale.The relationship between psychological capital and core competency was analyzed.Results The mean score of psychological capital was (4.365±0.773),the mean score of core competency was (5.379±0.744).There were significant positive correlations between psychological capital and core competency.Regression analysis showed that the self-efficacy,hope and optimism could explain 42.6％ of the variance for baccalaureate nursing students' core competency.Conclusions Nursing educator and administrator should focus on the shortcomings of psychological capital,and take effective measures to enhance nursing students' psychological capital and improve the level of core competency.

Objective To investigate the level and influencing factors of baccalaureate nursing students' core competency,and supply scientific reference for sustainable development of nursing career.Methods From May to June 2013,totally 280 baccalaureate nursing students from a third-level hospital in Tianjin were tested by the Chinese version of CINS edited by Li-Ling Hsu.Results The score of nursing students' core competency was (5.379±0.744),and the highest score was the moral and responsibility ability dimension (5.840 ±0.827),the lowest score was the critical thinking ability dimension (4.794 ± 1.105).Multiple linear regression analysis showed that ever finishing homework and preparing for the exam carefully,deciding the service units after graduation and gender were the influencing factors of nursing students' core competency.Conclusions Nursing students' core competency needs to be further improved.Nursing educators and managers should pay more attention to cultivate the nursing students' core competency,and develop strategies to enhance the level of baccalaureate nursing students' core competency.

The objective of the experiment was to separate neural stem cells from fetal rat brain by using immunomagnetic beads, and to study their morphology and marker on cell membrane. Cell suspension of the fetal rat cerebral cortex was incubated with antibody against Nestin and the labeled cells were separated through magnetic field by immunomagnetic beads coated with the second antibody. We determined the purity of the separated cells and the cell activity by light microscopy and detected the Nestin expression ratio of the sorted cells by flow cytomety. The morphology of neural stem cells was observed with a scanning electron microscope. The results showed that the purity of the separated cells was 87 8%%+2 4%, the activity of the cells was 94 8%+2 3%, and the Nestin expression ratio of the sorted cells was 88 3%+1 04%. Binding of the sorted cells to beads was shown under scanning electron microscope.Our conclusions are the magnetic cell sorting system we used can effectively separate neural stem cells from the cerebral cortex cell suspension and maintain their activity. The sorted cells can be used in the subsequent experiments.

Objective To further explore the effects of acupuncture and Fuyuan Mixture(FM) on learning and memory. Methods One hundred and twenty_nine NIH male mice were allocated to six groups:Group A (normal control group),Group B(model group),Group C(treated with acupuncture),Group D(treated with FM),Group E(FM and acupuncture),and Group F(treated with nimodipine). Model mice with acquired memory disorder were induced by scopolamine.Baihui,Dazhui,Shenting and Zusanli were chosen as the acupoints.FM is composed of Cornu Cervi Pantotrichum,Radix Ginseng,Rhizoma Polygonati,Fructus Lycii,Fructus Alpiniae Oxyphyllae,Radix Salviae Miltiorrhizae,etc..The methods of platform jumping and finding food in complex maze were used to observe their learning and memory. Results Compared with Group B,incubation period was prolonged,electric shock time shortened and errors reduced at 20 min and 24h after modeling in Group C and Group E,and time for finding food in complex maze was shortened in Group C and Group D(P

Objective To investigate the effects of sevoflurane ( Sevo ) on dendritic development and the expression of collapsin response mediator proteins ( CRMP ) in the hippocampus of developing rats. Methods Twenty-four neonatal Sprague Dawley (SD) rats at postnatal day 7 (P7) were randomly divided into control group or sevoflurane group ( 12 rat pups for each group) .Rats in the control group were exposed to air for 4 h,whereas rats in the sevoflurane group were exposed to 2.8%sevoflurane for 4 h.The hippocam-pus of some rats were collected,and the expressions of CRMP1,CRMP2 and CRMP4 proteins and phospho-rylation of CRMP2 protein at Ser522,Thr514 and Thr555 were detected by Western blot 6h after exposure ( n=6) .The rest rats were housed till P30,the expression of CRMP1,CRMP2 and CRMP4 proteins in the hip-pocampus were detected by Western blot ( n=6) and the morphology changes of dendrites in the dentate gy-rus ( DG) of hippocampal neurons were detected by Golgi-Cox Staining ( n=6) .Results The expression of CRMP1,CRMP2 and CRMP4 proteins of rats at P7 in the sevoflurane group was decreased by 35.0%( P=0.004) ,27.5%( P=0.015) and 12.0%( P=0.003) ,respectively,and the phosphorylation of CRMP2 pro-tein at Ser522 and Thr514 in the sevoflurane group were increased by 68.3%( P<0.01) ,74.5%( P<0.01) , respectively,6 h after exposure compared with control rats.However,the phosphorylation of CRMP2 protein at Thr555 was not significantly changed after sevoflurane exposure.At P30,both total dendrite length ( P=0.001) and the dendrites length at level 2 and 3 ( P=0.033, P<0.01,respectively) were shorter and the dendritic branching at 120,140 and 160 μm rings in Sholl analysis were less ( P=0.009, P=0.028, P=0.048,respectively) for rats in the sevoflurane group,compared with control rats.There were no significant changes at the expressions of CRMP1,CRMP2 and CRMP4 proteins.Conclusion Sevoflurane inhibits the development of dendrites in the hippocampal DG area of developing rats,which may be related to inhibition of CRMP1,CRMP2 and CRMP4 proteins expression and hyperphosphorylation of CRMP2 Ser522 and Thr514.

Objective To evaluate the reliability and validity of Chinese version of the competency inventory for nursing students(CINS).Methods The competency inventory for nursing students was translated and revised in Chinese version.Totally,266 baccalaureate nursing students were tested by the Chinese version CINS.SPSS17.0 was used to test the reliability and validity.Results The Cronbach' s α of CINS was 0.966 and Cronbach' sα of each dimensionality was ranged from 0.827 to 0.951.The Split-half reliability of Guttman of CINS was 0.828 and Split-half reliability of Guttman of each dimensionality was ranged from 0.724 to 0.942.The test-retest reliability was 0.737 and the content validity was 0.956.The correlation coefficients among dimensionalities were ranged from 0.491 to 0.781 (P＜0.01).The correlation coefficients between dimensionalities and the total scale were ranged from 0.738 to 0.884(P＜0.01).Five factors were extracted by exploratory factor analysis,accounting for 66.404％ of the total variance in the scale.Conclusion Chinese version of CINS is reliable and valid,and can be used to assess the competency of nursing students.

Objective To investigate the detection of BJ-75A-9 mHNA, WNX and CK19 mRNA in peripheral blood cells of lung cancer patients and evaluate its diagnostic value. Methods The expression of BJ-TSA-9, LUNX and CK19 mRNA in peripheral blood cells was delected from 84 lung cancer patients, 32 benign lung lesions and 20 healthy volunteers by nested reverse transcription polymerase chain re-action (Nested-RT-PCR). Results The positive detection rates of BJ-75A-9,LUNX and CK19 mRNA in the peripheral blood of the pa-tients with lung cancer were 59.5%,40.4%and 22.6% respectively. In the 32 peripheral blood samples of the patients with benign lung le-sions,the positive detection rate of BJ-TSA-9,LUNX and CK19 mRNA were 9.3%, 12.5% and 6.3% respectively. No expression of BJ-TSA-9,LUNX and CK19 was detected in lhe samples of the healthy volunteers. The expression level of BJ-TSA-9, WNX and CKI9 mRNA in the peripheral blood of lung cancer stage IV patients was significantly higher than those of stages II and ID (P< 0.05). Conclusion RT-PCR amplification of BJ-TSA-9, LUNX and CK19 mRNA are an efficient way to detect early haematogenous dissemination of cancer cells for lung cancer patients. The detection of BJ-TSA-9 expression is sensitive and specific for lung cancer,and it is superior to both LUNX and CK19 mRNA. The expression of BJ-7SA-9 mRNA in peripheral blood might predict the hematogenous metastatic spreading of lung cancer cells.

Objective To investigate the effects of acupoint catgut-embedding therapy(ACT) on hypothalamic of hypothalamic norepinephrine(NE),dopamine(DA),5-hydroxytryptamine(5-HT),5-hydroxyindole acetic acid(5-HIAA) in menopause rats,and to explore the preventive and therapeutic mechanism of ACT for climacteric syndrome.Methods Sixty female SD rats were equally randomized into treatment group,model group and sham-operation group.Except the rats in the sham-operation group,the rats in other groups received removal of bilateral ovaries to establish ovariectomized menopause rat models.The treatment group received catgut-embedding on acupoints of Shenshu,Sanyinjiao and Zigong,once per week,and the treatment lasted 4 weeks.After treatment,the hypothalamic NE,DA,5-HT,5-HIAA levels were measured by radioimmunoassay.Results NE and DA levels were significantly decreased(P

Objective To explore what brain regions are modulated by heroin addiction and withdrawal.MethodsWe used functional magnetic resonance imaging to investigate the brain function in 15 heroin-dependent patients 3 days (acute) and 1 month (protracted) after heroin abstinence.Sixteen normal controls were included.Results The blood oxygen level-dependent signal in the orbitofrontal cortex of the brain of heroin-dependent patients was significantly elevated 3 days after the withdrawal.Hyperfunction of the orbitofrontal cortex declined 1 month after the withdrawal.Conclusion Heroin-dependent subjects at both 3 days and 1 month abstinence have persistent abnormalities in the brain function.Although some tangible beneficial effects are noted following 1month of detoxification,possible permanent damage to the brain caused by heroin use is suggested.

Objective To examine white matter integrity in heroin-dependent patients and matched normal controls with diffusion tensor imaging (DTI).Methods The fractional anisotropy was compared between 15 heroin-dependent patients and 15 controls.Results We found the fractional anisotropy was significantly decreased in specific brain regions of the heroin-dependent patients (P ＜ 0.001 uncorrected),including the frontal gyrus,the parietal lobule,the insula,and the corpus callosum.Conclusion The presence of microstructural abnormality is found in the white matter of several brain regions of heroin-dependent patients.