Objective To investigate the effect of unilateral basal ganglia hemorrhage on long -term behavio-ral development of neonatal SD rats.Methods Forty -eight neonatal SD rats (1 0 days after birth)were equally divided into cerebral hemorrhage (CH)group,sham operation (SH)group and normal control (NC)group randomly, 1 6 cases for each group.Stereotaxic apparatus was used to inject autologous blood 25 μL into rats′brain caudate nu-cleus to establish basal ganglia hemorrhage model,while SH group was injected with nothing,and NC group received no treatment.Berderson scoring method was used to test rats′neurological functions on the first day,the third day,the seventh day,and the fourteenth day after operation,respectively.The open -field environment test and the Lat maze were used to assess behavior of the rats on the fourteenth day after operation,lasting for 3 days continuously.Results (1 )After being modeled rats appeared with different degrees of neurological function damage.SH group rats′neurologi-cal function damage was slight,which was completely restored in the 72 hours after surgery.CH group rats appeared hemiplegia and muscle tension change.CH rats neurological scores′on the first and third day after modeling scores were separate (3.40 ±0.83)scores and (1 .1 3 ±0.92)scores respectively,and the scores were statistically higher than SH group [(0.73 ±0.59)scores and (0.1 3 ±0.35)scores]and NC group (all P <0.05).And then Bederson score de-creased,and on the seventh and fourteenth day after modeling,the Bederson scores′difference was not statistically sig-nificant among the 3 groups(P >0.05).(2)On the fourteenth day after modeling,in open -field test and the Lat maze,the number of passed panels,straightening(times)and grooming(times)in CH group were more than those in SH group and NC group respectively (all P <0.05).The difference in the number of passed panels,straightening (times)and grooming (times)between SH group and NC group was not statistically significant (P >0.05 ). Conclusions Neonatal rats focal hemorrhage in unilateral basal ganglia is strongly repairable and compensatory,which leaves no severe neurological dysfunction.The neurological function damage which is caused by unilateral basal ganglia hemorrhage can lead to the increase of autonomic activities,the decrease of non -selective attention level,attention defi-cit and other long -term behavioral abnormalities.

Apoptosis is one of the most important causes, which results in the central neuronal system complication in hypoxic-ischemic brain damage (HIBD). Apoptosis occurs in the developing brain more than in the developed brain. Apoptosis can last several weeks and may be inverted its pathology by appropriate therapy. Caspase inhibitor, neurotrophic factors, anti-apoptosis gene Bcl-2, mild hypothermia,and early intervention play important roles in promoting neuronal cell survival and preventing from apoptosis through different mechanisms. It may be a new way for rehabilitation of HIBD. [

Objective To explore the influence of environment early in life on learning and memory abilities and neuroglobin (Ngb) expression. MethodsForty-five newborn,male,Sprague-Dawley rats were randomly divided into an EE (enriched environment) group,an EI (isolated environment) group and a normal control group,with 15 rats in each group.The EE and EI group rats lived in those environments for their first 28 days of life.The step-down test was used to measure the rats'learning and memory abilities on the 29th day.Ngb expression was examined using immunohistochemical methods. ResultsThe step-down test showed learning and memory scores for the EE group rats of (9.67±0.49) and (9.80±0.56) respectively,significantly higher than those of the control group,(8.67±0.72) and (8.93±1.10).The learning and memory abilities of the EI group were the lowest among the three groups,with scores of only (7.07±1.98) and (7.67±0.98 ),respectively.The latency of the first electric shock was (166.33±36.08)s in the EE group and significantly shorter than that in the control group (108.93±73.26) s.The EI group showed the longest average latency (44.93±45.03)s.Average Ngb expression as reflected by the integrated optical density in the EE group was (224.56±89.09) in the frontal cortex and (127.70 ± 28.16)in the hippocampus,a significant increase over expression in the control group.The Ngb expressions of the EI group were the lowest among the 3 groups-(88.78 ± 18.84) and ( 63.42 ± 16.41 ) in the frontal cortex and hippocampus respectively. ConclusionsEarly exposure to an enriched environment can increase Ngb expression and improve learning and memory ability,but early isolation will reduce Ngb expression and learning and memory function.

Objective To review the advantages and disadvantages of common attention deficit hyperactivity disorder(ADHD) animal models,published in 2000-2014 at home and abroad,with an attempt to provide new ideas for the future study of the pathogenesis in ADHD.Methods Major online database including CNKI,Wan Fang databases,OVID,Pubmed databases were searched in January 2015,using the key wordsattention deficit hyperactivity disorder, animal modelsand so on,to analysis the advantages and disadvantages of each type of experimental animal models and research value in ADHD.Results Totally 29 studies were enrolled,and the analysis show that the current commonly used experimental animal models of ADHD are spontaneously hypertensive rats,dopamine transporter gene knockout mice,lack of mutant mice model,neonatal 6 hydroxy dopamine damage of juvenile rat model,neonatal hypoxic rats model,X-ray irradiation injury model of rat hippocampus and other brain tissue damage model and isolated feeding model,and each animal model has the advantages,disadvantages and special research value of the pathogenesis of ADHD.Conclusion The specific pathogenesis of ADHD is unclear,but the neuroendocrine changes especially HPA axis attracted much attention in recent years.this paper summarizes the research at home and abroad comparison of several common animal models of ADHD.neuroendocrine changes in SHR and ADHD children have a certain degree of consistency in HPA axis,looking for ADHD children and SHR in neuroendocrine aspects of consistency is likely to open a new path to search the pathogenesis in ADHD.

Objective:To systematically evaluate the efficacy and safety of demethylation drugs intreatment of myelodysplastic syndrome (MDS), and to provide reliable basis and guidance for clinical application of these drugs.Methods:The randomized controlled trials (RCT) of demethylation drugs for treatment of MDS published in the PubMed, Web of Science, Embase, Cochrane Library, Chinese Journal Full-text Database (CNKI), Chinese Biomedical Literature Database (CBM) and VIP database from January 2000 to December 2019 were searched by computer. RevMan 5.3 software was used for Meta-analysis of the efficacy and safety.Results:Seven RCT studies involving 1 172 patients were obtained. Meta-analysis showed that the complete remission rate ( OR = 6.26, 95% CI 1.74-22.49, P = 0.005), partial remission rate ( OR = 4.65, 95% CI 1.51-14.29, P = 0.007), overall response rate ( OR = 14.14, 95% CI 7.27-27.51, P < 0.01), hematology improves ( OR = 3.47, 95% CI 1.44-8.32, P = 0.005) in the demethylation drug treatment group were better than those in the best supportive treatment group. Meanwhile, the overall survival of patients in azacytidine group was improved ( HR = 0.62, 95% CI 0.50-0.77, P < 0.01). In terms of adverse reactions, demethylation drugs increased the incidence of neutropenic fever ( OR = 4.19, 95% CI 2.26-7.76, P < 0.01). However, there was no significant difference in the incidence of neutropenia, thrombocytopenia, anemia, infection, nausea, liver damage and fatigue between the two groups (all P > 0.05). Conclusions:The effect of demethylation drugs in treatment of MDS is obvious, the remission rate can be improved, and azacytidine can prolong the patients' survival, but demethylation drugs increase the incidence of neutropenia fever. In the clinical application of demethylation drugs, it is necessary to further carefully evaluate their clinical safety.

90 ). ③For the experimental group at baseline, DQ of the gross movement was the highest (71.5?10.5), while that of the other four areas were similar (fine movement 56.7?10.4, adaptability 59.6?12.5, language 54.3?11.7, social 56.8?14.7).Conclusions:Early intervention had significant effects for the development of children with mental retardation, which medicine has less role.

Objective To study the effects of early intervention on functional outcome and content of growth associated protein (GAP- 43) in the brain of rats with hypoxic-ischemic brain damage (HIBD). Methods The animal model of intra-uterus HIBD was established in 30 pregnant Sprague-Dawley rats, who were then divided randomly into 4 groups: the intervention HIBD group (HIBD-IT),the non-intervention HIBD group (HIBD-NIT),the intervention control group(control-IT),the non-intervention control group(control-NIT). From the second day after the operation, the rats of the HIBD-IT and control-IT groups were kept in an enriched environment and administered with the neonatal handling including massage for 14 days. On the 28th day, jumping stand test was administered to all the rats, at the same time, the expression of GAP- 43 in brain of the rats in different groups were measured by immunohistochemical method. Results On the jumping stand test, the performance of control-IT and HIBD-IT groups were significantly better than that of the control-NIT and HIBD-NIT groups(P

Object: To explore the effects and the influent agents of early education on the healthy termed infants. Methods: 52 healthy termed infants voluntary to the exercise center as experimental group were early educated. The other 52 infants as control group were in the same elementary conditions comparing to the experimental group. The experimental group was trained the corresponding projects and examined DQ at the beginning and the 3 rd,6 th,9 th month after training. The control group was only examined DQ at the beginning and after 9 months. Results:①At the 9 th month after training, DQ of the experimental group was improved 21.9?13.6, which was much 7 times higher than that of the control group, which was improved 3?3.0 (P

Objective: To explore the functions of psychological intervention in the integrative therapy for children's hematological tumor. Methods: The psychological status of 52 children with hematological tumor and their parents, and 48 healthy children and their parents were measured by means of the self-compiled psychological status scale and the symptom checklist 90 (SCL-90). Moreover, 26 children with hematological tumor and their parents, who were drawn out in random, were done by psychological intervention of 3 months as well as chemotherapy, and then their psychological status were evaluated. Results: The psychological problems were more prevalent in children with hematological tumor and their parents than those in the healthy children and their parents. Among the total, the problems of social behavior (3.9?2.9), personal emotion (7.1?4.7), habit (1.2?1.1), and learning (1.3?1.2) in the children with hematological tumor were more than those(1.5?1.6, 1.5?2.0, 0.5?0.7, 0.4?0.5) in the healthy children(P

Objective:To investigate the features of children's visual cognitive function of attention deficit hyperactivity disorder (ADHD).Methods:By Benton Visual Retention Test (BVRT) including three means of C, D, and E, Trail Making Test, and Symbol-Digit Modalities Test (SDMT), 50 cases of ADHD and 50 cases of normal children were examined in their visual consciousness, visual memory, visual space structure, visual motion attention, and visual scanning et al.Results:①By the means of BVRT, the correct score was significantly lower in ADHD group (4.9?2.0)than in control group(7.7?1.4, t=8.00,P