Based on the problems existing in the original specimen management process of the First Affiliated Hospital of Guangdong College of Pharmacy,the paper proposes achieving the closed-loop management process of laboratory specimens through wireless network technology,PDA,laboratory specimen bar code,QR codes of patients and interaction of multiple systems,and describes the functional design and application effect of the system.The application of the closed-loop management process for laboratory specimens can better standardize and normalize the management of specimens.

Objective To explore the effect of risk management on the perioperative nursing of elderly patients undergoing cardiovascular surgery. Method Sixty-two elderly patients undergoing cardiovascular surgeries were managed by management, which included establishing risk management team, identity and evatuating risk factors, applying risk manadement. Result There was no nursing risk events among them. Conclusion For elderly patients with cardiovascular surgery, risk management can enhance nurses′ability of discerning, assessing and managing the nursing risks so that it is effective in avoiding possible risks, reducing nursing adverse events during perioperative period, improving nursing quality and ensuring the safety of surgery.

Objective To investigate the diagnostic threshold value and the influencing factors of high‐sensitivity troponin I measurement in the diagnosis of patients with acute myocardial infarction .Methods Collecting 9 236 patients who are the first time hs‐cTnI testing in the internal emergency department of our hospital(including 9 099 patients with non‐AMI and 137 patients with AMI) as participants ,then dividing non‐AMI patients into elder group(>60 years old) and younger group(≤60 years old) ,hs‐cTnI concentrations were compared between the elder and the younger group with different gender .The optimal threshold value of hs‐cT‐nI for acute myocardial infarction was determined by receiver operating characteristic(ROC) curve of AMI patients with different age and gender .Results The hs‐cTnI concentrations of non‐AMI patients in the elder male group were 39 .21 ng/L [(37 .48-40 .93)ng/L] ,which was higher than those of non‐AMI patients in the younger male group 22 .38 ng/L[(21 .16-23 .6)ng/L] and the difference was statistically significant(P<0 .05) ,the same result was seen in the elder female group and the younger female group .The total male group was 31 .26 ng/L [(30 .17 -32 .34)ng/L] ,it was higher than the total female group 26 .8 ng/L [(25 .73-27 .86)ng/L] .According to the ROC curve ,the optimal threshold value for AMI diagnosis was 45 ng/L in total AMI group and 135 ng/L in the elder male group and 45 ng/L in the elder female group ,which was different with the cut off value(120 ng/L) .Conclusion the hs‐cTnI levels were related with age and gender ,it is very important for us to consider these factors when we make the optimal threshold value with our own data .

Objective To investigate the characteristics of bone mineral density (BMD) in the patients with maintenance hemodi‐alysis(M HD) and relevant influencing factors to achieve the purpose of early diagnosis of osteoporosis .Methods Seventy‐two pa‐tients undergoing MHD and 76 healthy individuals(control group) were selected .The BMD values of lumbar spine and hip were measured in all subjects by using the dual energy X‐ray absorptiometry(DEXA) .The abnormal situation of BMD and the change of serum bone metabolism indicators in the patients with M HD were analyzed .Then the related factors affecting BMD were further ex‐plored .Results Compared with healthy controls ,the average BMD of lumbar and hip joints in M HD patients was significantly de‐creased ,which in elderly women was most remarkable ,and the incidence of osteoporosis was also significantly increased (P<0 .05) . Meanwhile ,the serum detection results showed that the levels of AKP ,iPTH ,P ,osteocalcin(OC) ,procollagen 1 N‐terminal peptide (P1NP) and β‐crosslaps (β‐CTX) in the NHD patients were significantly increased ;however the levels of Vit D and Ca were markedly decreased .The correlation analysis results showed that BMD was positively correlated with BMI and blood Ca ,while neg‐atively correlated with age ,dialysis age ,serum AKP and OC levels .Conclusion M HD patients are easily to appear the osteoporosis phenomenon ,in which the gender ,age ,dialysis age ,BMI ,serum AKP ,Ca and OC are the factors affecting BMD .

Smooth muscle cell (SMC) proliferation, platelet free calcium level and aggregation of experimental atherosclerotic rabbits were investigated in this study. Aortic SMC ofhyperlipidemic rabbits in vitro showed higher growth activity than did normal rabbit SMC. And also hyperlipidemic serum stimulated SMC to proliferate at a significantly greater rate than control serum. Moreover, the level of platelet free calcium and the platelet aggregation was also higher in hyperlipidemic rabbits, indicating that activitated platelets possibly release more PDGF to act as a stimulator to SMC proliferation and calcium is an important factor to activate platelets. Furthermore, SMC from 8501-treated rabbits appeared lower proliferative rate than thecells from hyperlipidemic rabbits. And serum from those rabbits inhibited SMC proliferation compared with hyperlipidemic serum, the inhibitory effect was even stronger than that of normal serum. It may be relevant to the favorable effects of 8501 to TXA2/PGI2 balance.

[Objective] To optimize the preparation technology of Maodongqing Drop Pill (MDP). [Methods] Orthogonal design was used to observe the influence of preparation conditions such as dry extract content, dry extract gram size, gel forming matrix and cooling agents on the quality of MDP. [Results] MDP made under the optimized conditions was good in shape, and color and luster, and not adhesive, indicating the good quality of the product. [Conclusion] This optimized preparation technology of MDP is simple with satisfied technique parameters and is suitable for mass production in the enterprises.

Objective To analyze the clinical features of hyperperfusion syndrome occurring after carotid artery stenting, and to discuss its nursing measures. Methods Among 220 patients who received carotid artery stenting, nine developed hyperperfusion syndrome after stent implantation. Their clinical materials were retrospectively analyzed. The nursing measures, including properly controlling blood pressure, relieving brain edema, monitoring cerebral blood flow, medication with sedation drug, stopping or reducing antiplatelet therapy, close observation of blood pressure and clinical symptoms, cooperation with physicians to control the blood pressure and to dynamically make reexamination, etc. Results Of the nine patients with hyperperfusion syndrome, complete recovery was achieved in eight at the time of discharge and death due to intracranial hemorrhage occurred in one. Conclusion The key point of nursing for patients with hyperperfusion syndrome is close cooperation with physicians to control the patient ’s blood pressure so as to ensure a proper cerebral blood flow.

Objective To study the effect of computerized pressure hemostat on lower limb deep vein thrombosis in high-risk elderly patients.Methods From September to December 2013,36 orthopedic hospitalized patients who received operations were divided into the hemostat group and the non-hemostat group with 18 patients in each group.D-dimer changes and lower limb deep vein thrombosis were observed in both groups.Results D-dimer was significantly increased in both groups compared with that before operation,but the extent of elevation in the non-hemostat group was lower than that in the hemostat group.D-dimer and cases of lower limb deep vein thrombosis in the hemostat group was higher than those in the non-hemostat group after operation,which showed significant difference.Conclusions The use of computerized pressure hemostat will increase the risk of lower limb deep vein thrombosis in elderly patients,so the technical operation procedures should be strictly enforced accompany with safely use of computerized pressure hemostat.

Objective To study the clinical features of hyperkalemic periodic paralysis (hyperKPP) and the relationship with SCN4A gene in a Chinese family Methods The clinical features of 7 patients in a Chinese family with hyperKPP were summarized All 24 exons of SCN4A gene were screened with denaturing high performance liquid chromatography (DHPLC) technology, and then sequence analysis was performed on those with abnormal elution peak Results This family showed typical clinical features of hyperKPP but without myotonia Three mutations were found in exon 13, 23 and 24 respectively Linkage analysis and direct sequencing showed the mutation in exon 24 was a synonymous mutation The mutation in exon 23 was a missense mutation, but proved to be a benign polimophism; the mutation in exon 13 was proved leading to the best known amino acid exchange Thr704Met Conclusion SCN4A gene should be related to hyperKPP, and Thr704Met be responsible for hyperKPP in this Chinese family

Objective To study the effect of nursing intervention on preoperative anxiety of patients with gynecological carcinoma.Methods 60 patients with gynecological carcinoma in our hospital who were to receive operations were randomly distributed into the control group and the intervention group with 30 in each group.The control group was given routine nursing,while the intervention group received nursing intervention based upon routine nursing.Indexes such as preoperative anxiety,sleeping quality and satisfaction degree to nursing care were evaluated within them.Results Through targeted nursing intervention preoperative anxiety of patients in the intervention group alleviated,their confidence enhanced and satisfaction degree to nursing care improved compared to those of the control group.Conclusions Targeted nursing intervention can reduce preoperative anxiety of patients with gynecological carcinoma and increase satisfaction degree to nursing care.