Dens invaginatus is an unusual malformation.The difficulty in recognizing a tooth with dens invaginatus frequently results in the delayed diagnosis followed by unpromising treatment outcome because the abnormality cannot be seen until the tooth is involved with pulp necrosis even apical periodontitis.The application of CBCT scanning and dental microscope is playing a significantly important role in both diagnosis and treatment of dens invaginatus.This paper presents 2 cases with dens invaginatus which were well diagnosed and properly trea-ted,reviews the pathology and intends to get the principle ideas for the diagnosis and treatment of dens invaginatus.

Objective To observe the clinical efficacy of acupuncture plus Nao Xue Shu oral liquid in treating vascular dementia (VD) due to blood stasis blocking collaterals. Method A total of 135 patients with VD due to blood stasis blocking collaterals were randomized into a basic group, a medication group, and an acupuncture-medication group, 45 cases in each group. The basic group was intervened by conventional treatment, while the control group was additionally given Nao Xue Shu oral liquid, and the acupuncture-medication group was additionally intervened by acupuncture plus Nao Xue Shu oral liquid, 60 d as a treatment course. The Mini-mental State Examination (MMSE), Brief Screening Scale for Dementia (BSSD) and the syndrome of blood stasis blocking collaterals subscale in the syndrome differentiation scale of vascular dementia (SDSVDA) were scored before and after the treatment, and the changes of calcitonin gene-related peptide (CGRP), lipid peroxidation (LPO) and serum uric acid (SUA) were also observed. Result One case dropped out in the basic group. After 60-day treatment, the total effective rate was 91.1%(41/45) in the acupuncture-medication group, significantly higher than 65.9% (29/44) in the basic group (P<0.01) and 75.6% (34/45) in the medication group (P<0.05). The improvements of MMSE, BSSD and SDSVDA scores in the acupuncture-medication group were more significant than those in the basic group and medication group (P<0.05). The improvements of CGRP, LPO and SUA levels in the acupuncture-medication group were superior to those in the basic group and medication group (P<0.05). Conclusion Traditional acupuncture method plus Nao Xue Shu oral liquid can produce a significant efficacy in treating VD due to blood stasis blocking collaterals.

OBJECTIVE To analyze the morbidity,pathogens,risk factors and prevention management measures.METHODS The data of the infected patients,reasons,bacteria isolates and antibiotic susceptibity tests were retrospectively analyzed.RESULTS A total of 277 patients(11.32%) among the 2248 patients during 3 years were infected in the hospital.The frequency of hospital infecton was 13.44%.Respiratary tract infection(57.14%) was the most common part,31.00% in upper respiratory tract infection and 26.14% in lower respiratory tract.G-bacilli(78.56%) were the predominant bacteria.G+ cocci(47.06%) were the predominant bacteria isolated from meningitis patients.CONCLUSIONS The hospital infection rate in neurosurgery department is higher than other departments.The risk factors of hospital infection are invasive procedure,antibiotic usage and hypoimmunity.

Objective To study the effect of crystallins on survival and growth of rat retinal ganglion cells (RGCs) in vitro and study elementarily the exact substance of the injured lens promoting RGCs survival and axonal regeneration. Methods RGCs in Long Evans rats were cultured in DMEM, crystallins and crystallins activated macrophage-conditioned media. The growth regularity and survival time of RGCs in vitro were observed under phase-contrast microscope. The number of RGCs with processes, length of the longest processes and cells activity were measured when RGCs were cultured for 1, 3 and 5 days respectively. Results (1) RGCs could survive for 12-14 days in DMEM containing crystallins, and the survival time of RGCs was longer significantly than other two groups (P

By analyzing major characteristics of professionalism education for medical students in certain western developed countries,this paper proposes some measures to strengthen the current professionalism education for medical students in China.

Humeral condylar fracture is one of the common clinical fractures,the treatment is difficult for it often accompanied with their joint surface injury,Learn from past experience combined with our department for humeral condylar fracture treatment,this article was designed to review the different treatment of humerus fractures,analyze the advantages and disadvantages of each treatment and indications for detail,with an emphasis on the application of artificial elbow Joint replacement in the humeral fractures.With a view to enhance the understanding to the treatment of Humeral condylar fracture.

OBJECTIVE: To determine the relationship between the ear protective measures during winter swimming and the prevalence, severity of the incidence of external auditory canal exostoses. METHOD: Three hundred and fifteen people who enjoyed swimming were recruited as research subject (62.2% males and 37.8% females; age distribution: 4.4% were ≤ 40, 40.3% were 41 to 60, and 55.2% were > 60 years). People fill in the questionnaires including personal information, the ear protection measures, the time of winter swimming, the history of ear infections and so on, and questionnaires were correlated with otoscopic findings. We classify external auditory canal exostoses according to the degree of the stenosis of external auditory canal s. Grades of normal,mild, moderate and severe corresponded to 100%, 99% to 66%, 65% to 33%, and less than 33% respectively. RESULT: Of 110 ears with exostoses, 63.6% were mild, 23.6% were moderate, and 12.7% were severe. Among the group of without ear protection during winter swimming, about 33.0% have auditory canal exostoses and 4.6% were severely affected. In comparison, in the group that had ear protection, only 2.8% had external auditory canal exostoses and no one had severely obstructed auditory canal. CONCLUSION A negative association exists between the ear protection measures during winter swimming and the prevalence and severity of exostoses of the external auditory canal. That means taking ear protective measures when having winter swimming can prevent the onset of the external auditory canal exostoses and reduce its severity.
Adult ; Age Distribution ; Constriction, Pathologic ; Ear Canal ; pathology ; Ear Diseases ; epidemiology ; Exostoses ; epidemiology ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Prevalence ; Seasons ; Surveys and Questionnaires ; Swimming

Objective To establish a new ELISA method by use of shorted recombinant antigen pp65 for detection of IgM antibodies to human cytomegalovirus (HCMV).Method According to HCMV pp65 sequence of nucleotide and amino acid, the shorted pp65 was decided by computer soft ware of protein and attained through gene engineering technique. The by computer soft ware of protein and attained through gene engineering technique. The recombinant enzyme- linked immunosorbent assay (REC-ELISA ) method was developed using pp65 recombinant protein as antigen and was applied to detecting anti-HCMV-IgM in sera. It was compared to ELISA by use of whole virus as antigen and Biocheck CMV IgM enzyme immunoassay test kit.Results To detect HCMV IgM by REC-ELISA, the best quantity of pp65 was 3.5?g percent cavity, the best dilution of HRP~*anti-HCMV IgM was 1∶[KG-*2]800 and that of serum was 1∶[KG-*2]100. The positive coefficient of variation (CV) was 9.5% in stability assay. The average CV of Inter- assay and Intra-assay was 4.5% and 9.6% respectively. The positive value of REC- ELISA was 44%, that of ELISA using whole virus was 50% and BioCheck was 45%. REC-ELISA using most suitable condition was concordant with BioCheck, which was 97.0%. Its youden′s index was 92.8% and its specificity (98.2%) was higher than that of ELISA using whole virus as antigen (90.9%), too.Conclusion REC-RLISA had good sensitivity, specificity and reproducibility. The method was easy and quick. Recombinant protein was harmless and easy gained compared with the whole virus. It can be large-scale production and standardization. Therefore, the application value and potential of REC-ELISA was very large.

Objective To discuss the effects of vitamin C in vitro,on human cancer HeLa cell multiplication and apoptosis, in order to promote vitamin C in the tumor treatment application. Methods the morphology changeof cervical cancer Hela cell was observed under the inverted microscope;the effects of vitamin e on Hela cell by MTT assay; cell cyele and weakly perishing rate was analysed rate was analysed by Flow CytoMeter;applying the agarose gelatin electricity swims to analyse weakly perishes in the process the cell gene group DNA change. Results Vitamin C hinders the HeLa cell by the density and the time dependence way to the G0/G1 period, suppresses it to grow and to induce weakly perishes. Conclusions Vitamin C may suppress the HeLa cell to grow, causes the cell to hinder to the G0/G1 period and induces cell weakly perishing.Its reason possibly was HPV E6 protein expression surrenders to restoring the function of P53 gene.

Aim To study tumor suppressor gene OPCML,DAPK methylation changes during the process of CasKi cell apoptosis induced by trichosanthin, and to explore the correlation and the role between cervical cancer cell apoptosis and tumor suppressor gene methylation so as to find new demethylation drugs.Methods ① MTT was applied to assay the inhibition of TCS to CasKi cell proliferation and flow cytometry was used to analyze cervical CasKi cell apoptosis induced by trichosanthin; ② Methylation-specific PCR(MSP) technology was applied to detect cervical cancer and during cell apoptosis process OPCML and DAPK gene promoter methylation status of CpG islands.Results In CasKi cervical cancer cells,OPCML and DAPK gene promoter region showed a high degree of CpG island methylation status, by trichosanthin treatment,the growth of CasKi markedly was inhibited, and flow cytometry analysed the characteristic sub-G1 peak,OPCML and DAPK gene promoter region showed no CpG island methylation of performance.Conclusions During the process of CasKi cell apoptosis induced by trichosanthin,OPCML and DAPK gene demethylated significantly,accordingly, trichosanthin might be a new methylation inhibitor,and there might be some correlation between cell apoptosis and tumor suppressor gene methylation.And OPCML and DAPK gene methylation tests might become new clinical indicators for the early detection of cervical cancer.