Objective To investigate the characteristics of contact allergens in eczema and dermatitis for further diagnosis and treatment. Methods 340 cases with eczema and dermatitis were tested with a standardized screening antigen kit. Results (80. 88% ) of the 340 patients tested had at least one positive allergen among 18 of 20 standardized screening antigen. The first six allergens were nickel sulfate ( 29. 70% ), potassium dichroroate (27.64% ) ,fragrant mix(22.35% ) ,carba mix(20.29% ) ,thimerosal( 18. 82% ) ,benzocaine( 17.94% ). Conclusion Nickel sulfate,potassium dichromate,fragrance mix and carba mix were the main allergens causing dermatitis and eczema presently. Patch-testing was helpful in identifying the property of contact allergens in para eczema and dermatitis.

Objective To establish the mathematical model and perform experimental mensuration on effective slice width for 4-slice spiral CT. Methods First, assuming the spiral slice sensitivity profile (SSP) was Gauss distribution, a formula about effective slice width, defined as the full width at half maximum (FWHM) of SSP, was get. Then, using Philips Mx8000 4-slice spiral CT system and the same CT technique as those used for clinical CT, CTP 528 of Phantom Catphan 500 was scanned with parameters as follows: 120 kV, 150 mAs, 180 mm FOV, 512×512 Matrix, 180° linear interpolation, different pitch and 0.5 mm detector width. Finally, FWHM was got from the coronal multiplanar reconstruction image passing by the center of tungsten ball in CTP528 for each sequence. Results With pitch of 0.500, 0.625, 0.875, 1.000, 1.250, 1.500 and 1.750, the measured FWHM was 0.7, 0.7, 0.7, 0.8, 0.7, 0.7 and 0.7 mm, respectively. With detector width of 0.5, 1.0 and 5.0 mm, the measured FWHM was 0.7, 1.2, and 6.7 mm, respectively. All the measured effective slices were higher than those of theoretical values. Conclusion The relativity between effective slice width and detector width is direct proportion, while that between effective slice width and pitch is non-linear.

Objective To observe the clinical effect of drug moxibustion in the treatment of chronic obstructive pulmonary disease (COPD).Methods A total of 90 patients with acute myocardial infarction in Taihe Hospital emergency department were randomly divided into the control group (n=30) and the treatment group (n=90).The patients in the control group were treated with routine western medicine, while treatment group was treated with drug moxibustion on the basis of the control group treatment. Both groups were treated for 6 weeks.The pulmonary function was assessed, and the clinical effect was evaluated.Results The total effective rate was 86.7% (52/60) in the treatment group and 70.0% (21/30) in the control group. The difference between the 2 groups was statistically significant (χ2=6.059,P=0.048). After treatment, the forced expiratory volume in 1 second (FEV1) (1.07 ± 0.3l L vs. 1.05 ± 0.41 L,t=15.272) in the treatment group were significantly higher than that in the control group (P<0.01).Conclusions The drug-separated moxibustion can improve the curative effect and improve the lung function of patients with stable COPD.

Objective To explore the effects of stress management on the quality of life ( QOL) of patients with chronic pelvic pain syndromes .Methods By convenience sampling method , 60 cases of patients with chronic pelvic pain syndromes in the urology department , were divided into the experimental group and the control group with 30 cases in each group .Patients in the control group were given routine nursing and guidance , while patients in the experiental group were given stress management intervention and health education guidance . National institutes of health-chronic prostatitis symptom index (NIH-CPSI) and quality of life measurement scale ( WHOQOL-BRIEF) were used to measure some index before and after intervention .Results Before the intervention, there was no sigificant difference in the scores of WHOQOL-BRIEF and NIH-CPSI between two groups ( P>0.05 ) .After intervention , the scores of physiological field , psychological field , social field and environment in QOL in the experimental group were (67.85±18.81), (59.37±15.36), (65.27±13.28) and (63.34±17.59), and they were all obviously higher than that in the control group with (51.27±14.96),(56.62± 17.28),(58.36±16.72) and (57.94±14.93) (t=3.276,3.732,2.365,3.210;P<0.05).The total NIH-CPSI score of the experimental group were (16.20±2.18), which was significantly lower than that of the control group (27.16±2.06) (t=19.947,P<0.05).Conclusions Stress management can notablely improve the QOL and symptornsof patients with chronic pelvic pain syndromes .

Objective: To understand the characteristics of sports load intensity of junior middle school physical education, and its relation with students’ physical fitness, and to provide suggestions for scientific arrangement of sports load of physical education. Methods: A total of 88 students from three grades in a middle school in Guangzhou were randomly selected. Polar heart rate tester was used to monitor the students’ heart rates during class, and physical fitness test was conducted on the students to obtain their physical fitness scores. Descriptive analysis, correlation analysis and one-way analysis of variance were used to process the data. Results: From the static characteristics, in junior high school physical education class, the mean of the lowest heart rate was 86.94 times/min, the mean of the average heart rate was 142.55 times/min, the mean of the maximum heart rate was 192.66 times/min, the average heart rate of junior grade one students was significantly lower than that of junior two students and junior three students (F=3.64，P<0.05). There was no significant difference in average heart rate between boys and girls (F=0.83，P>0.05); In terms of dynamic characteristics, heart rates at 3 and 31 minutes were significantly lower than those at 10, 17 and 24 minutes (F=13.87，P<0.01),in each part of junior high school physical education class, there were significant differences between grades, specifically, the heart rate of junior students was significantly lower than that of senior students (F=9.85，P<0.01); In terms of the correlation between exercise intensity and physical fitness, there was a significant negative correlation between the lowest heart rate, average heart rate, maximum heart rate and physical fitness(r=-0.34，-0.32，-0.29，P<0.01). Conclusion The physical activity intensity of junior middle school students is moderate and the average heart rate is within the range stipulated by "Chinese healthy physical education curriculum model".

Objective:To report embryonic testicular regression syndrome(ETRS) caused by DHX37 heterozygous variant for the first time in China and summarize the clinical manifestations of ETRS as to improve the understanding of doctors for this disease.Methods:The clinical data and whole exome sequencing results of five cases of ETRS from Shenzhen Children′s Hospital were collected. The reported cases of DHX37 heterozygous variant were reviewed.Results:Five patients with ETRS visited the doctors at the age of 2 months to 5 years and 5 months. Three patients raised as males came to hospital due to virilition and 2 female patients visited a doctor due to clitoral hypertrophy. No uterus was detected by ultrasound in all patients. The gonadal pathologies from 4 cases displayed no testicular tissue or gonadal dysgenesis, complicated with gonadoblastoma in one case. The genetic testing revealed that the heterozygous variant(c.923G>A, p. R308Q) in DHX37 was found in 2 cases, without variant in other 3 cases. According to the review, ETRS and 46, XY gonadal dysgenesis due to DHX37 herozygous variant was firstly reported in 2019. A total of 40 cases, including 21 cases of ETRS, presented with the virilition or female phenotype, with the disappearance of testicular tissue as the main pathologies. There is no report in China.Conclusion:The article summarized the clinical manifestations and whole exome sequencing results of 5 patients with ETRS, among which two cases were caused by DHX37 variants and one was complicated with gonadoblastoma.

Objective To compare the safety,efficacy and complications of laparoscopic pyelolithotomy (LPL) and percutaneous nephrolithotomy (PCNL) for treatment of renal pelvic stones larger than 2.5 cm.Methods From 2011 to 2016,32 patients underwent LPL and another 32 patients received PCNL for renal pelvic stones larger than 2.5 cm.The baseline characteristics of the patients,stone size,mean operative time,estimated blood loss,postoperative hospital stay,stone-free rate,postoperative analgesia,blood transfusion,and the intraoperative,early postoperative and long-term complications were compared between the two groups.Results The baseline characteristics and stone size were comparable between the two groups.The mean operative time of LPL and PCNL was 117±23.12 and 118.16±25.45 min,respectively (P＞0.05).The two groups showed significant differences in the mean estimated blood loss (63±11.25 vs 122±27.78 mL,P＜0.01) and blood transfusion rate (0 vs 6.2％,P＜0.01) but not in postoperative hospital stay (4.5±1.34 vs 4.8±2.2 days,P＞0.05),stone-free rate (93.1％ vs 87.5％,P＞0.05) or the postoperative analgesia time (1.7± 0.5 and 1.9 ± 0.6 days,P＞0.05).The incidence of intraoperative complications were significant lower in LPL group than in PCNL group (6.2％ vs 25.0％,P＜0.01),but the incidences of early postoperative complications (25.0％ vs 34.4％,P＞0.05) and long-term postoperative complications (9.4％ vs 12.5％,P＞0.05) were sinilar between them.Conclusion PCNL is the standard treatment for pelvic stones larger than 2.5 cm,but for urologists experienced with laparoscopic technique,LPL provides a feasible and safe option for management of such cases.

Objective To compare the safety,efficacy and complications of laparoscopic pyelolithotomy (LPL) and percutaneous nephrolithotomy (PCNL) for treatment of renal pelvic stones larger than 2.5 cm.Methods From 2011 to 2016,32 patients underwent LPL and another 32 patients received PCNL for renal pelvic stones larger than 2.5 cm.The baseline characteristics of the patients,stone size,mean operative time,estimated blood loss,postoperative hospital stay,stone-free rate,postoperative analgesia,blood transfusion,and the intraoperative,early postoperative and long-term complications were compared between the two groups.Results The baseline characteristics and stone size were comparable between the two groups.The mean operative time of LPL and PCNL was 117±23.12 and 118.16±25.45 min,respectively (P＞0.05).The two groups showed significant differences in the mean estimated blood loss (63±11.25 vs 122±27.78 mL,P＜0.01) and blood transfusion rate (0 vs 6.2％,P＜0.01) but not in postoperative hospital stay (4.5±1.34 vs 4.8±2.2 days,P＞0.05),stone-free rate (93.1％ vs 87.5％,P＞0.05) or the postoperative analgesia time (1.7± 0.5 and 1.9 ± 0.6 days,P＞0.05).The incidence of intraoperative complications were significant lower in LPL group than in PCNL group (6.2％ vs 25.0％,P＜0.01),but the incidences of early postoperative complications (25.0％ vs 34.4％,P＞0.05) and long-term postoperative complications (9.4％ vs 12.5％,P＞0.05) were sinilar between them.Conclusion PCNL is the standard treatment for pelvic stones larger than 2.5 cm,but for urologists experienced with laparoscopic technique,LPL provides a feasible and safe option for management of such cases.

Objectives The combined use of bedaquiline and delamanid(BDQ-DLM)is limited by an increased risk of prolonging the QTc interval.We retrospectively evaluated patients who received DLM/BDQ-containing regimens at a TB-specialized hospital.We aimed to present clinical efficacy and safety data for Chinese patients. Methods This case-control study included patients with multidrug-resistant tuberculosis(MDR-TB)treated with BDQ alone or BDQ plus DLM. Results A total of 96 patients were included in this analysis:64 in the BDQ group and 32 in the BDQ+DLM group.Among the 96 patients with positive sputum culture at the initiation of BDQ alone or BDQ combined with DLM,46 patients(71.9%)in the BDQ group and 29(90.6%)in the BDQ-DLM group achieved sputum culture conversion during treatment.The rate of sputum culture conversion did not differ between the two groups.The time to sputum culture conversion was significantly shorter in the BDQ-DLM group than in the BDQ group.The most frequent adverse event was QTc interval prolongation;however,the frequency of adverse events did not differ between the groups. Conclusion In conclusion,our results demonstrate that the combined use of BDQ and DLM is efficacious and tolerable in Chinese patients infected with MDR-TB.Patients in the BDQ-DLM group achieved sputum culture conversion sooner than those in the BDQ group.

Teaching in experiments of biology is important for the cultivation of life science talents. In view of the rapid development of life science and the increasing demand for research-oriented talent training, teaching in experiments of biology should set up a variety of learning outcomes: to train experimental skill, to cultivate students' experimental design and operation abilities, and to improve students' scientific thinking and innovative consciousness. We have carried out an educational reform on experimental genetic engineering blended course. In this paper, we introduced our methods of organizing online materials, the curriculum design of the blended course, the implementation details, and a preliminary analysis of teaching effects. We found that experimental genetic engineering blended course could support students' active learning and a learning-centered teaching model. Moreover, it could facilitate students' achievement of improving experimental skills, cultivating a rigorous scientific attitude, professional research quality and academic innovation ability.
Biological Science Disciplines ; Curriculum ; Genetic Engineering ; Humans ; Students