Aim To explore the protective role of schisandrin B (SchB)against HK-2 damage induced by Cisplatin and the probable mechanism.Methods Cultivated HK-2 was interacted by Cisplatin and SchB with different concentrations.Invert microscope showed cellular form change and Flow Cyto Meter told apopto-sis.Combined with test result on the change of L-DH, MDA and LPO,probable protective effect of SchB on HK-2 was studied and analyzed.Result Cisplatin in-hibited cells′growth and induced apoptosis.20 μmol · L-1 SchB significantly reduced apoptosis of Cispla-tin-induced HK-2,and offered the best protection. SchB could reduce MDA,LPO,LDH significantly in-creased by cisplatin.Conclusion SchB can be an ef-fective inhibition agent of Cisplatin-induced renal tubu-lar epithelial cell apoptosis (HK-2 ).The mechanism can be the inhibition of Cisplatin-induced oxidative stress by antioxidant effect.

Humans ; Stomach Neoplasms ; pathology

OBJECTIVEThis research aimed to fabricate a hydroxyapatite (HA)-chitosan scaffold via simulated body fluid (SBF) biomimetic mineralization and determine how mineralization time affects scaffold construction and cell compatibility.

METHODSThe HA-chitosan scaffolds were fabricated by freeze-drying technique and then subjected to precalcification, also known as alternative soaking method. Afterward, precalcificated scaffolds were placed into the SBF to conduct the mineralization process. Mineralization time was set at 7, 14, and 21 days, corresponding to three experimental groups. Pure chitosan scaffolds acted as the control group, and the physical and chemical properties of the four groups were tested. Osteogenic-induced adipose-derived stem cells (ADSCs) were seeded into the scaffolds to investigate the scaffolds' cell compatibility.

RESULTSThe mineral substance of the 14-day group exhibited a uniform distribution. The crystal composition of the mineral substance suited the HA's features. The compressive elastic modulus increased along with the extension of mineralization time. The 21-day group showed a statistically significant increase in compressive elastic modulus compared with the control group (P < 0.05). The cell proliferation level of the 14-day group was significantly the highest among the three experimental groups (P < 0.05). The calcium ion and the type I collagen had the highest secretion amount when the cells were seeded into the 14-day group.

CONCLUSIONThe SBF biomimetic mineralization method can be used to fabricate HA-chitosan bone-tissue-engineering scaffolds. The biological compatibility, as well as the chemical and physical properties, reached the optimum levels at day 14.

Objective To study the chemical constituents of bamboo root.Methods The chemical constituents were isolated by chromatographic methods and their structures were elucidated by analysis of spectral data and physicochemical properties.Results Nine compounds were isolated and identified as 3- (p-hydroxyphenyl)-(2,3) oxirane-1-propionic acid(Ⅰ),2,3-dihydroxy-1-(4-hydroxy-3,5-dimethoxy-phenyl) -1-propanone(Ⅱ),(+)-lirioresinol B(Ⅲ),protocatechuic aldehyde(Ⅳ),protocatechuic acid (Ⅴ),orientin(Ⅵ),vitexin(Ⅶ),homoorientin(Ⅷ),and isovitexin(Ⅸ).Conclusion CompoundⅠis a new compound named epoxy coumaric acid.

Objective To discuss the main high-risk factors,clinical features and prognosis of global developmental delay(GDD),so as to provide effective basis for reducing incidence of children with GDD,early diagnosis,early intervention and improving prognosis.Methods One hundred and eighty-five cases of children with GDD,who were first diagnosed and treated in the Pediatric Neurology Rehabilitation Center,the First Affiliated Hospital of Anhui Medical University from October 2011 to September 2013,were included and high-risk factors,clinical features,and prognosis were analyzed.At the same time,the patients were followed up for 2 years and the children with abnormal development received continuous intervention and treatment during the follow-up.x2 test was used to compare high-risk factors and prognosis of different clinical features and Logistic regression models were selected to analyze high-risk factors influencing prognosis.Results In 185 cases with GDD,there were 119 children (64.3％) with motor and language developmental delay,which were the most common features,and followed by types of motor combined cognitive and language developmental delay which make up 30 cases (16.2％) and cognitive merged language developmental delay which make up 22 cases (11.9％) and the rarest type of 14 cases (7.6％) was motor and cognitive developmental delay.The main high-risk factors included neonatal asphyxia,premature birth,pathologic jaundice,intrauterine growth retardation,intrauterine hypoxia,neonatal hypoxic-ischemic encephalopathy (HIE),neonatal infection and pregnancy-induced hypertension syndrome and the differences of various clinical features with premature birth,intrauterine growth retardation,pathologic jaundice were statistically significant.Up to 2 years of follow-up,40 cases (21.6％) turned normal,but 145 children (78.4％) were still abnormal,including 97 children (52.5％) having significantly improved after intervention,30 cases(16.2％)of intellectual developmental disorder and 18 cases (9.7％) of cerebral palsy.The differences in various clinical features showed statistically significance (x2=60.960,P=0.017).The main high-risk factors affecting prognosis were intrauterine growth retardation [β=0.777,odds ratio (OR)=2.174],intrauterine hypoxia (β=0.706,OR=2.026),HIE(β=0.547,OR=1.729) and neonatal asphyxia (β=0.070,OR =1.073).Conclusion Causes of GDD are complex and prognosis is poor and the etiology and prognosis of children with different clinical features are also different.It is important to enhance perinatal care,early diagnosis and intervention for reducing the incidence of GDD and improving prognosis.

A 3-year-old boy presented with a 3-month history of brown-yellow papules scattered on the trunk.The first skin biopsy showed a dermal infiltrate of many mononuclear histiocytes,eosinophilic granulocytes and a small number of lymphocytes.Immunohistochemical examination of the lesions demonstrated positive reactions with anti-CD68,-S100 and-CD1a (partial) antibodies.After the biopsy,the skin lesions gradually turned dark and partially regressed leaving hyperpigmentation,but new lesions continuously appeared.Four months later,a second biopsy was performed,and showed a dermal infiltrate of histiocytes with eosinophilic granulocytes and a few multinucleated giant cells.Immunohistochemistry showed that the histiocytes stained positive for CD68,but negative for S100 and CD1a.Based on the above findings,the patient was diagnosed with juvenile xanthogranuloma.

OBJECTIVE To probe cleaning methods of reusable puncture needles and improve the quality of cleaning. METHODS The 200 sets of puncture needles with blood were divided into two groups (groups A and B). Ultrasonic cleaning was used in two groups,but in group A was with 3% hydrogen peroxide solution and in group B with 1∶150 low foam multi-enzyme cleaning liquid for 20 minutes,after rinsing,the group A used 95% ethanol 2 ml/piece for dry,but the Group B used 90 ℃ 30 min,and then the bacterial endo toxins and 3% hydrogen peroxide tests,the cost of cleaning was compared. RESULTS The positive rates of the bacterial endo toxins and 3% hydrogen peroxide test in group A were conducted,at last were 6% and 5%,while group B all were negative (?2=6.19,5.13,P

OBJECTIVE To improve the management quality by discussing the usage of "Five Regular Methods" in hospital′s Sterilization Supply Center.METHODS The "Five Regular Methods" managment model into the following aspects of quality,service,safety,efficiency,people′s competency and environment was introduced.RESULTS After 6 months implementation of the model the efficiency and quality of service improved significantly.The environment became clean and shiny.Objects were put on their right place. And we realized the right integration in time and space for people,objects and places.CONCLUSIONS The "Five Regular Methods" is a set of perfect guidance for quality management.It is a management system by providing guidance from inside to outside,people to objects,soft ware to hard ware,theory to practice and rules to process.It is a good method for enhancing the service quality from all aspects.

Objective To explore the effect of psychological intervention on patients with advanced ovarian cancer. Methods A total of 80 patients with advanced ovarian cancer patients combined with carboplatin or Western him paclitaxel combined with cisplatin in the treatment of patients, the implementation of grouping according to nursing, patients in the control group for routine care, patients in the observation group in the control group on the basis of psychological nursing. The two groups were observed and compared the clinical effect of intervention. Results The observation group the effective rate of the treatment and nursing satisfaction rate were significantly higher than the control group; the implementation of group nursing, observation group patients psychological status and improve the psychological status of patients in the observation group after treatment were better, and the control group have obvious difference. Conclusion For patients with advanced ovarian cancer chemotherapy, combined with psychological intervention on the basis of routine nursing, can significantly improve the treatment efficiency, improve the psychological status of patients, is worthy of clinical application

Objective To explore the value of the combined detection of helper T lymphocyte (Th)1 and 2 type cytokines ex‐pression in the evaluation of acute pancreatitis severity and treatment efficacy .Methods From May 2009 to June 2013 ,the enzyme‐linked immunosorbent assay (ELISA ) was adopted to detect the expression levels of Th1‐type cytokineinterferon‐γ (IFN‐γ) and Th2‐type cytokine interleukin (IL)‐4 in 32 cases of mild acute pancreatitis (MAP group) and 18 cases of severe acute pancreatitis (SAP group) on 1 ,4 ,7 d after admission as well as 15 cases of normal healthy people (control group) .Results The test results of IFN‐γ on 1 ,4 ,7 d after admission in the MAP group were (31 .47 ± 4 .35) ,(36 .92 ± 4 .12) ,(49 .09 ± 4 .97)ng/L ,which of IL‐4 were (54 .72 ± 5 .52) ,(45 .26 ± 4 .89) ,(39 .79 ± 3 .85)ng/L .The test results of IFN‐γ on 1 ,4 ,7 d after admission in the SAP group were (23 .97 ± 5 .39) ,(26 .29 ± 3 .47) ,(33 .64 ± 4 .47)ng/L ,and which of IL‐4 were (70 .45 ± 5 .05) ,(65 .99 ± 4 .58) ,(56 .23 ± 4 .23)ng/L .The test results of IFN‐γ and IL‐4 in the normal control group were (49 .26 ± 20 .67)ng/L and (35 .15 ± 16 .32)ng /L respectively .Compared with the normal control group ,the expressions of Th1‐type cytokine IFN‐γ in the MAP group (except which on 7 d) and peripheral blood Th1‐type cytokine IFN‐γ in the SAP group were decreased and the expression of Th2‐type cyto‐kine IL‐4 was increased ,the difference was statistically significant (P< 0 .05) ;The test results of each index in the same treatment time had statistically significant differences between the SAP group and the MAP group (P< 0 .05) .Conclusion The obvious po‐larization of Th1‐type cytokine and Th2‐type cytokine exists in acute pancreatitis ,and the polarization degree is related with the se‐verity of disease .The combined detection of Th1‐type cytokine and Th2‐type cytokine is beneficial to judge the extent of serious condition and treatment effect of acute pancreatitis .