AIM: To investigate the growth inhibition effects of CDA-II on breast cancer cells. METHODS: The effects of CDA-II on growth curve and morphology of breast carcinoma cell lines MCF-7 and MDA-MB-231 were observed in vitro cultures. RESULTS: CDA-II reduced the growth speed and proliferation ability. CONCLUSION: CDA-II has remarkable effects on anti-cell-proliferation and shows good prospects of application in the treatment of patients with breast carcinoma.

Objective:To investigate the effect of rosuvastatin on carotid arterial intima-media thickness of elderly patients and its comparison with simvastatin.Method:126 patients hospitalized during January and August 2008 were randomly divided into a treatment group and a cotrolled group.All the patients were male and older than 70 years old,and their mean age was 78.9?8.8 years.63 patients accepted the rosovastatin therapy and 63 patients accepted the simvastatin therapy.Result:After 8-weeks'therapy,CIMT was reduced from 1.58?0.24mm before the treatment to 1.44?0.22mm after it.There was no difference between the two groups in decreasing CIMT.No adverse events related to the study medication were observed.Conclusion:The treatment with rsuvastatin can prevent the increase of carotid arteriosclerosis in eldlerly patients without any difference in decreasing CIMT,compared with simvastatin.

Objective To explore the influence of nursing intervention on patients with schizophrenia receiving repetitive transcranial magnetic stimulation (rTMS) treatment.Methods A total of 80 patients with schizophrenia receiving (rTMS) treatment were randomly divided into intervention group 40 cases and control group 40 cases by random digits table method.The control group was given routine care, and the intervention group was given routine nursing and nursing intervention.Before and after treatment, the curative effect was evaluated by the Positive And Negative Symptoms Scale, and the completion of the treatment between two groups was compared.Results The Positive Scale score, Negative Scale score, General Psychopathology Scale, total score in intervention group after treatment were lower than those in control group:(13.1±2.3) points vs.(16.7±2.1) points,(11.5±2.6) points vs.(15.3±2.3) points, (28.3±2.3) points vs.(30.9±2.2) points,(56.9±1.8) points vs.(67.4±1.7) points, and there were significant differences, t=2.398,2.401,2.452,2.191 ,P＜0.05.There was no significant difference in the rTMS completion, x2=5.541, P＜0.05.Conclusions Nursing intervention can effectively improve the patients with schizophrenia completion of rTMS treatment to improve mental symptoms, so as to improve the clinical curative effect, promote patients recovery of disease.

Objective To determine that atorvastatin might have anti-inflammatory effects in acute coronary syndromes(ACS) with C-reactive protein(CRP) reduction.Methods Ninety-two patients with ACS were assigned to three groups: high dose atorvastatin group(31 cases),taking atorvastatin 40 mg daily.Standard dose atorvastatin group(31 cases),taking atorvastatin 10 mg daily,and control group(30 cases),only receiving conventional therapy.CRP levels,lipid profiles were measured at first and fifth day and 1 month later.Results The study suggested:(1)CRP levels significantly decreased from baseline to the fifth day and 1 month later in high dose atorvastatin group(P

Objective To investigate the role of vascular cell adhesion molecule 1 (VCAM 1) in pathogenesis of pregnancy induced hypertension (PIH) complicated by fetal growth restriction (FGR). Methods The placenta samples were collected from 30 pregnant women with PIH complicated by FGR, 28 FGR patients without PIH and 30 normal pregnant women. Immunohistochemical analysis was used to demonstrate the positive expression of VCAM 1 in placenta vascular endothelium and syncytiotrophoblast of these samples. Results In placentas of PIH complicated by FGR (80%),expression of VCAM 1 in the decidual vascular endothelium was significantly higher than those without PIH (25%) and normal placentas (15%)( P

Objective To investigate the expression of HLA DR on the platelet membrane and the activation of CD4 +T lymphocyte with idiopathic thrombocytopenic purpura (ITP) Methods By flow cytometry, HLA DR expressed on platelet membrane and IL 2 level produced by activated CD4 +T lymphocyte in 60 patients with ITP and 60 normal volunteers were measured Results The percentage of platelet expressing HLA DR (0 808?0 218)% measured in 60 patients with ITP by flow cytometry analysis was markedly higher than that in control subjects (0 025?0 019)% ( P

Complications of vascular access are main component of vascular complication in percutaneous cardiovascular intervention.The common complications of femoral access may include hemorrhage and hematoma at access site,vasovagal reflex,pseudoaneurysm,arteriovenous fistula,retroperitoneal hematoma and deep venous thrombosis.The common complications of radial access may include radial artery spasm,radial artery occlusion,vascular injuries or hematoma at forearm and other sites,and osteofascial compartment syndrome.The common complications of ulnar and brachial accesses may include hemorrhage and hematoma at access site,upper limb and hand ischemia,and nerve injuries.Effective prevention and treatment of vascular access complications are key steps to minimize the incidence and hazards of vascular complications in percutaneous cardiovascular intervention.

Objective To study the effect of mitochondrial membrane potential and cellular membrane permeability in the neurotoxicity of artemether. Methods Mitochondrial membrane potential and cellular membrane permeability in pheochromocytoma cell and primary cultured rat neuronal cell were measured by flow cytometry, using rhodamine 123(Rh123) and PI as fluorescent dye, respectively. Mitochondrial swelling was measured by spectrometer. Results Artemether could decrease the mitochondrial membrane potential and increase the cellular membrane permeability in both cell types in a dose-dependent manner. In addition, artemether led to mitochondrial swelling with dose-effect and time-effect relationships. Conclusion The decrease of mitochondrial membrane potential plays an important role in the neurotoxicity of artemether. The drug can change the mitochondrial membrane potential and mitochondrial swelling by affecting the permeability transition pore complex located in the mitochondrial membrane, increasing cellular membrane permeability, causing an obstruction and finally leading to neurotoxicity.

Objective To study the relationship between matrix metalloproteinase-9 in syncytiotrophoblast and placenta pathological change of fetal growth restriction. Methods The placental samples were collected from 30 patients with FGR and 30 normal pregnant women who delivered in our hospital from January 2001 to September 2001. The tissue sections were dealt with HE stain and PAS stain and then observed by microscope to find the morphological change. 5 another samples from each group were observed by 500H model electron microscope. Immunohistochemical analysis was employed to demonstrate the positive expression of the MMP-9 in syncytiotrophoblast of FGR placenta. Results There are significantly pathological change in 22 placentas of FGR and abnormal rate is 73.33%. The count of stroma fibrosis and fibrinoid necrosis of villi(63.3%), villous vascular decrease and gore(70.0%), villous syncytial knots(43.3%), cytotrophoblasts increase(50.0%) were more significant in the placenta of FGR group than that of normal group(6.7%,10.0%,3.3%,13.3%)(P

Objective To estabilish a HPLC method for the determination of Geniposide in Jigucao Capsule. Methods The column was Lichrospher-C18(5 ?m,4.6 mm?250 mm). The determination was carried out with acetonitrile-0.1% phosphoric acid(10∶90) as the mobile phase,a flow rate of 1.0 mL/min and detected at 238 nm,and temperature of the column was 35 ℃. Results The calibration curve showed good linearity in the range of 0.14～1.68 ?g,Y =1 552.15X-6.42,r=0.999 9. The mean recovery was 97.53%(RSD=1.24%,n =6). Conclusion The method is accurate,reliable,and can be used for quality control of the preparation.