Objective To improve the objectivity and consistency of microscopically comparison in morphology by using SP-1000i and CellaVision DM96.Methods Ten cases of venous blood samples were collected.The morphological comparison of white blood cells were performed between the traditional method and the new method (by using SP-1000i and CellaVision DM96),at the same time,between 2 veterans and other 10 common operators.According to the same methods,the time was compared.Results By using this new method,the consistency of microscopically comparison was greatly improved (P<0.01),the number of people who need to compare again was decreased obviously(P<0.01),and the time of the procedure was also shortened evidently(P<0.05).Conclusion The quality control of clinic laboratory will must be greatly improved with timesaving and labour-saving when SP-1000i and CellaVision DM96 are applied in microscopically comparison in mor-phology.

Objective To investigate the effects of gemcitabine,a cytotoxic drug,combined with photodynamic therapy (PDT) in treatment of human pancreatic cancer xenograft in nude mice.Methods The animal model of human pancreatic cancer was developed by suturing small pieces of SW1990 tumors into the dorsum of nude mice.Sixty animal models were randomly divided into five groups with 12 each:control group ( without any treatment ), photosensitizer group ( 2 mg/kg of Photosan, without illumination ),chemotherapy group (receiving 50 mg/kg of gemcitabine i.p. on day 0,3,6 and 9 after transplantation),photodynamic group (2 mg/kg photosan combined with laser irradiation) and combination group (50 mg/kg of gemcitabine and 2 mg/kg of photosan combined with laser irradiation). The tumor sizes were measured twice every week.All mice were sacrificed after 21 days.The tumor volume was calculated,and inhibitory effect and changes before and after treatment were analyzed.Results The tumor was grown bigger in control,photosensitizer and chemotherapy groups (all P value ＜0.05).On day 6,9,12,15,18and 21, the tumor size was significantly smaller in photodynamic group than those in control and photosensitize groups.While the tumor size on day 18 and 21 was smaller in combined group than those in photodynamic group(all P value ＜0.05). The tumor mass was (0.29 ± 0.20) g in combined group,which was lower than that in photodynamic,control,photosensitize and chemotherapy groups[(0.69±0.23) g,(1.65±0.21) g,(1.62±0.12) g,(1.37±0.19) g,respectively,P＜0.05].The inhibitory effect were 82.420%00 and 58.18% in combined group and photodynamic group,respectively(P＜0.05),while there was 1.80% and 17.00% in photosensitize and chemotherapy groups,respectively.Conclusions Photodynamic therapy has significant and short anti-tumor effect,but it can be significantly enhanced by combined with small dose of gemcitabine.

Objective To investigate the anti-tumor effects of photodynamie therapy(PDT)on human pancreatic cancer xenograft in nude mice and the time-effect relationship of PDT.Methods The animal model of human pancreatic cancer was developed by suturing small pieces of SW1990 tumors into the dorsum of nude mice.When the size of the tumors increased to 0.8～1.0 cm.36 mice were randomly divided into three groups:control group(no treatment),photosensitizer group(Photosan 2mg/kg,abdominal cavity injection),photodynamic group(Photosan 2 mg/kg injection+laser irradiation).Each group included 12 mice.The tumor sizes were measured twice per week and the weight8 and volumes of the tumors were measured,and the tumor inhibitory rate was calculated three weeks later.Results The tumor volume of photodynamic group was (0.22±0.12)mm3 at the 6th day,which was significantly smaller than(0.43 s0.18)mm3 of control group and(0.39±0.15)mm3 of photosensitizer group(P＜0.05).15 days later,the tumor volumes of PDT groups increased.21 days later.the weight of the tumors in photodynamie group was(0.69±0.23)g,which was significantly lower than(1.65 ±0.21)g of control group and(1.62±0.12)g of photosensitizer group(P＜0.05).The tumor inhibitory rate in photodynamic group was 58.18%,which was significantly higher than that of photoseasitizer group(1.8%,P＜0.05).Conclusions Photodynamie therapy had significant anti-tumor effect on human pancreatic cancer with quick-acting efficacy,but photodynamie therapy alone exeaed efficacy only in the shoa term.

Objective To investigate the curative effect of Aidi injection solution combined withcarboplatin on controlling malignant pleural effusion(MPE).Method A total of 86 cancer patients with appearedPME were selected as our subjected who hospitalized in the First People's Hospital of Shangqiu from Feb.2012to Mar.2013.They were randomly divided into the observation group(43 cases) and the control group(43cases).Patients in the oontrol group were treated with carboplatin at dose of 100 mg/per times which was putinto the thoracic cavity,and patients in the observation group was treated with Aidi injection solution at dose of100 mg/per times beside carbeplatin.The treatment period was 4 weeks.The effect information,quality of lifeand adverse reactions were recorded.Results The total effective rate and survival quality improvement rate inobservation group were 86.05％ (37/43) and 51.16％ (22/43),higher than those in control group (67.44％(19/43),30.23％ (13/43)),and the differences were significant(x2 =4.170,3.903 ; P ＜ 0.05).There wasno significant difference in terms of the related adverse reactions between two groups(P ＞0.05).ConclusionThe therapy of Aidi injection solution plus carboplatin on controlling MPE is proved to be effective and it canimprove patients' quality of life with better safety.Therefore it is worthy of recommendation.

Objective To investigate the expression of HLA DR on the platelet membrane and the activation of CD4 +T lymphocyte with idiopathic thrombocytopenic purpura (ITP) Methods By flow cytometry, HLA DR expressed on platelet membrane and IL 2 level produced by activated CD4 +T lymphocyte in 60 patients with ITP and 60 normal volunteers were measured Results The percentage of platelet expressing HLA DR (0 808?0 218)% measured in 60 patients with ITP by flow cytometry analysis was markedly higher than that in control subjects (0 025?0 019)% ( P

A series of multinuclear diethylenetriamine ligands were synthesized and used as artificial nuclease enzyme model. Target compounds were characterized by 1H NMR, 13C NMR, IR and ESI-MS. Preliminary studies on the cleavage of pUC19 DNA in the presence of metal complexes have also been performed and the results revealed that these complexes could act as powerful catalysts for the cleavage of pUC19 DNA after 48 h under physiological conditions. The hydrolytic cleavage mechanism of DNA plasmid by title compound was confirmed by T4 DNA ligase experiment.

Objective To study the changes of Prealbumin (PA),C-reactive protein (CRP) and D-dimer (DD) levels in septic shock patients due to pneumonia so as to explore the relation to the severity of disease and prognosis.Methods PA,CRP and DD levels of 48 sepsis patients were measured on 1st 、 3rd、5th day after admission and the last day before discharge or death.Meanwhile APACHE Ⅱ score were documented.According to prognosis,patients were divided into death group and non-death group.Results The levels of PA、CRP and DD on the 1 st day were of no significant difference between the death group and non-death group( t = 2.315 ,P ＞ 0.05;t =2.013,P ＞0.05; t =2.511 ,P ＞0.05).The level of PA in death group on the 3rd day,the 5th day and the last day was much lower than that in non-death group ( t = 2.422,P ＜ 0.05; t = 2.557,P ＜ 0.05; t = 2.906,P ＜ 0.05 ).The level of CRP and DD in death group on the 3rd day,the 5th day and the last day was much higher than those in nondeath group(t =2.061 ,P ＜0.05 ;t =2.843,P ＜0.05 ,t =3.519,P ＜0.01 and t = 2.576,P ＜0.05,t = 2.823,P ＜0.05; t = 3.371 ,P ＜ 0.01 ).There was a negative correlation between PA and APACHE Ⅱ scores ( r = - 0.631,P ＜0.01 ) ,while a positive correlation was found between CRP,DD and APACHE Ⅱ score(r =0.687 ,P ＜0.01 and r =0.651 ,P ＜ 0.01 ).Conclusions Persistent low level of PA and high level of CRP,DD indicated severe disease course and unflavored prognosis.It is of clinical value to evaluate the severity of disease and predict prognosis in sepsis by combined dynamic observation of PA,CRP and DD levels.

Objective To observe the clinical efficacy and safety of application of lafutidine treated in curing peptic ulcer.Methods Eighty-four patients with peptic ulcer were randomly assigned to receive either lafutidine(10 mg) twice daily in 42 patients of cured group or lansoprazole (30 mg) once daily for 4 weeks in 42 patients of control group.Ulcer healing rate and clinical performance were examined after treatment.Results The first,third and seventh day of peptic ulcer healing rate were 38.09％ (16/42), 71.42％ (30/42) and 90.47％ (38/42) in the lansoprazole group and 23.81％ (10/42), 76.19％ (32/42) and 85.71％ (36/42) in the lafutidine group.There were no siginificant difference between two groups (P =0.157,0.620, 0.736).Gastroscope examination showed the complete healing rate, average healing rate and general clinical effectiveness were 61.90％(26/42) ,30.95％(13/42) and 97.61％(41/42) in the lansoprazole group,while the number for lafutidine group were 57.14％ (24/42), 28.57％ (12/42) and 95.23％ (40/42).There were no siginificant difference between two groups (P =0.657, 0.811, 1.000).No severe side-effect was occurred in both groups.Conclusion Use Lafutidine to cure peptic ulcer can promote healing effectively, improve patients' clinical symptoms which is similar to lansoprazole.

Objective To systematically study the development and morphological changes of Taenia solium cysticerci in the period from the appearance to maturation. Methods Pigs were orally infected with T.solium eggs. Autopsies were made periodically to get tissue specimens and examine the distribution of cysticerci. Cysticerci were excised, pressed between two microscopic slides for microscopical observation. Sections were also made for histological study. Results Cysticerci distributed mainly in muscles, secondly in brain, liver, lung and kidney. The size of cysticerci was highly variable at different parts or even in the same part of the same host. The early stage cysticercus was observed on the 19th day without suckers and hooklets on the scolex region, and was only found in skeletal muscles. Histological examination revealed that early development of the scolex became obvious. No cysticerci were found in other tissues. At 30 d, the cysticerci possessed hooklets and brood suckers. With a longer duration of infection, the diameter of suckers and length of hooklets both increased. Larger suckers and more folds on scolex appeared 60 d later. Morphologically, cysticerci after 60 d were similar to those found at 60 d. Degenerated cysticerci were found at every stage. Conclusion Cysticerci appear in about 19 days after the pig was infected with eggs of T.solium and the duration needed for maturation is about 60 d.

Objective:To observe the protective effect of Shenkang injections in the rats with kidney injury induced by gentamicin. Methods:Forty-eight male SD rats were randomly divided into the control group,model group and treatment group with 16 ones in each. The rats were given gentamicin with intraperitoneal injection(100 mg·kg-1 ·d-1 )for 7 days in the model group and the treat-ment group,and the rats in the treatment group were given Shenkang injections with intraperitoneal injection additionally(2. 0 g·kg-1 ·d-1 ). Eight rats were sacrificed respectively on the 8th and 15th day in each group,and the detection of serum level of creatinine and cystatin-C,urinary concentration of kidney injury molecule-1(Kim-1)and neutrophil gelatinase-associated lipocalin(üGAL),activity of nuclear factor-κβ and Caspase-3,and expression of Bax mRüA and Bcl-2 mRüA in renal tissues as well as the observation of renal tissue apoptosis in each group were conducted. Results:Compared with those in the control group,the serum level of creatinine and cystatin-C,urinary concentration of Kim-1 and üGAL,renal tissue apoptosis,activity of nuclear factor-κβ and Caspase-3,expression of Bax mRüA and Bcl-2 mRüA in renal tissues on both 8th and 15th day were higher in the model group(P<0. 01). The serum level of creatinine and cystatin-C,urinary concentration of Kim-1 and üGAL,renal tissue apoptosis,activity of s nuclear factor-κβand Caspase-3,expression of Bax mRüA in renal tissues in the treatment group were lower at the two time points than those in the model group, while the expression of Bcl-2 mRüA in homogenate renal tissues were higher in the treatment group than that in the model group at the two time points(P<0. 01). All indices were higher at two time points in the treatment than those in the control group. Conclusion:Shenkang injections exhibit protective effect in rats with kidney injury induced by gentamicin through inhibiting renal tissue apoptosis.