ObjectiveTo establish a method for the determination of Nuezhenide and Apiganin 7-O-neohesperidoside in Shanxiangyuan tablets.Methods The Luna C1s column was used,the mobile phase consisted of HCN-0.5％H3PO4(41 ∶ 59),the flow rate was 1.0 ml/min,the column temperature was 30℃,the UV detector was set at 336nm.Results The linear response range was 0.016～0.32 mg/ml (r=0.9997) and 0.006～0.11 mg/ml respectively.The average recovery rate was 99.0％ and 97.5％ respectively,RSD was 1.83％ and 1.57％ respectively,ConclusionThe method was simple,accurate and repeatable.It can be applied in quantitative determination of Nuezhenide and Apigenin 7-O-neohesperidoside in Shanxiangyuan tablets.

Objective To compare the difference in cost formation between hemodialysis and peritoneal dialysis so as to provide basis for the formulation and perfection of medical security policies. Methods An analysis was made of the cost formation and profits of hemodialysis and peritoneal dialysis in 4 hospitals of Shanghai in 1999. Results Of the average cost of each performance of hemodialysis, the cost of materials accounted for 35% to 47% and the cost of equipment accounted for 38% to 44%, while the scope of increase in profits was affected both by the number of patients and the average profits per performance. Of the average cost of each performance of peritoneal dialysis, the cost of materials exceeded 97% while profits were related only to the number of patients. Conclusion There is a marked difference in profits between the two forms of dialysis, leading to an improper expansion in the use of hemodialysis and an inhibition in the rational use of the technique of peritoneal dialysis.

Objective:To elucidate the signal transduction mechanism of the modulation of immune cells by estrogen,the effect of 17?-estradiol(E2)on the production of nitric oxide (NO)from spleen mononuclear cells(MNC)of rats and the expression of inductible NO synthase(iNOS) in estrogen receptor(ER) knockout mice were examined.Methods:Culture of the spleen MNC from normal,Freund's incomplete adjuvant(FIA)or myelin basic protein(MBP) immunized Lewis rats.Detection NO production in the supernatant of culture was determined by Griess reagent.The expression of iNOS in spleen sections were observed by immunofluorescence staining.Results:①E2 dose-dependently induce NO production in spleen MNC from normal rats,MNC from FIA or MBP immunized rats manifest in the same manner.iNOS INHIBITOR l-NAME or MAPK blocker could suppress the induction.②In vivo E2 enhanced iNOS expression in wild type mice(WT),but had no effect in ER ? knockout mice(EPKO).ER? knockout mice(BERKO)appear the same with wt.Conclusion:E2 could modulate the immune system via induction of NO,which involves both genomic and nongenomic mechanisms.

Objective To investigate the effects of sea voyage on cellular immune function and nutrition status of naval servicemen. Methods A total of 173 male naval servicemen were included in the present study. The percentage of T, B and NK cell subpopulations from peripheral blood were assessed by flow cytometry. CD3+, CD3+CD4+, CD3+CD8+ and CD4+/CD8+ were employed as the markers of T cell subpopulation, CD3-CD19+ served as the marker of B cell subpopulation, and CD3-CD56+ served as the marker of NK cell subpopulation. The serum total protein (TP), albumin (ALB), glucose (Glu), total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HLD-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein A1 (ApoA1), apolipoprotein A2 (ApoA2), apolipoprotein B (ApoB), apolipoprotein C2 (ApoC2), apolipoprotein C3 (ApoC3), apolipoprotein E (ApoE), lipoprotein a (LPa), Fe2+, total iron-binding capacity (TIBC), Hb, K+, Na+, Cl-, Ca2+ and Mg2+ levels were determined by 7600DDP. Results Compared with that before sailing, the percentage of CD3+, CD3+CD4+ and CD4+/CD8+ elevated significantly during voyage (P

ObjectiveTo evaluate the anti-glaucoma effect of 1％ D-timolol on patients with primary open-angle glaucoma （POAG） and ocular hypertension （OH）．MethodsThirty-six patients with POAG or OH were included．They were divided into two groups randomly for treating with either 1％ D-timolol or 0.5% L-timolol twice daily．The intraocular pressure（IOP） changes and side effects on eyes and cardiovascular system were noticed．Data of visual field and ocular blood flow were compared with the baseline after given the eyedrops for 3 month．ResultsAfter given one drop of the drug，intraocular pressure dropped significantly in both of the two groups．The IOP day curve at 1 month also dropped when compared with the baseline in both groups．There was no significant difference between the two drugs in magitude of hypotensive effect after 3 months．And the higher the IOP was，the more the IOP dropped when using D-timolol．There were no serious side effects noted．No significant change of ocular blood flow was found by color doppler imagine．Mean sensitivity of visual field was raised significantly in D-timolol-treated group，while in L-timolol-treated group it was not changed much．Conclusion1％ D-timolol is a favorable eyedrop. It is effective and safe for the treatment of glaucoma．

OBJECTIVE: To analyze the recurrencerelative factors of early glottic carcinoma after carbon dioxide laser treatment. METHOD: A retrospective analysis of 134 early glottic carcinoma patients' clinical data was taken to analyze the relationships between recurrence and gender, age, pathologic degree, T stage, involvement of anterior commissure and involvement of thyroarytenoid muscle. RESULT: Recurrent ratio of well-differentiated, middle-differentiated and poorly-differentiated squamous cell carcinoma were 11.83%, 18.42% and 33.33% (P > 0.05) respectively. Recurrent ratio of T1a, T1b, T2 were 13.24%, 18.92%, 23.08% (P > 0.05) respectively. The recurrent rate was 33.33% of tumors offended the anterior commissure, versus 5.43% with no involvement of anterior commissure (P < 0.01). The recurrent rate was 59.09% of tumors offended the thyroarytenoid muscle, versus 5.36% with no involvement of thyroarytenoid muscle (P < 0.01). Unvaried and multivariate analysis indicated that the involvement of anterior commissure and the involvement of thyroarytenoid muscle were the factors of recurrence. CONCLUSION Recurrence of early glottic carcinoma after carbon dioxide laser treatment is effected by the involvement of anterior commissure and the involvement of thyroarytenoid muscle. Resection can be performed conservatively according to evaluation of macroscopic tumour extension.
Carcinoma, Squamous Cell ; surgery ; Glottis ; Humans ; Laryngeal Neoplasms ; surgery ; Laryngectomy ; Laser Therapy ; Lasers, Gas ; Microsurgery ; Neoplasm Recurrence, Local ; Retrospective Studies

A rapid method has been developed based on the sample preparation procedure named as QuEChERS (Quick,Easy,Cheap,Effective,Rugged and Safe),combined with reversed-phase high performance liquid chromatography with fluorescence detector and C18 column after precolumn derivatization using o-phthalaldehyde and 2-mercaptoethanol to determine dopamine in porcine muscle.Methanol and deionized water (0.1％ acetic acid,v/v) with a ratio of 60∶40 was used as mobile phase.The flow rate was 0.8 mL/min and dopamine was eluted within 15 min.The linearity range was 0.003-8 μg/mL with r=0.9992.The detection limit for dopamine was 4 μg/kg and the quantification limit was 9 μg/kg.Recovery studies were carried out at 0.1,0.5 and 1.0 mg/kg fortification levels and the average recoveries obtained ranged from 90.4％ to 98.2％ with relative standard deviations between 3.5％ and 8.1％.The method was found to be suitable for detection of dopamine in animal product tissues at the maximum residue level.

A rapid method has been developed based on the sample preparation procedure named as QuEChERS （Quick, Easy, Cheap, Effective, Rugged and Safe）, combined with reversed-phase high performance liquid chromatography with fluorescence detector and C18 column after precolumn derivatization using o-phthalaldehyde and 2-mercaptoethanol to determine dopamine in porcine muscle. Methanol and deionized water （0.1% acetic acid, v/v） with a ratio of 60：40 was used as mobile phase. The flow rate was 0.8 mL/min and dopamine was eluted within 15 min. The linearity range was 0.003-8 μg/mL with r=0.9992. The detection limit for dopamine was 4 μg/kg and the quantification limit was 9 μg/kg. Recovery studies were carried out at 0.1, 0.5 and 1.0 mg/kg fortification levels and the average recoveries obtained ranged from 90.4% to 98.2% with relative standard deviations between 3.5% and 8.1%. The method was found to be suitable for detection of dopamine in animal product tissues at the maximum residue level.

Diabetic foot infection may hamper wound healing and lead to non-traumatic amputation in diabetes patients and early diagnosis and proper treatment can improve prognosis.In this review,the progress in classification,influencing factors,diagnostic methods and local treatment of diabetic foot infection is introduced.

Objective To evaluate the role of several traditional laboratory examinations in identifying the cause of WBC count increase,which might be the adverse reaction of glucocorticoid or infection.Methods 74 patients treated in the hospital from Jan. 2011 to Dec.2012 were recruited in the study.Glucocorticoid without Infection(n =15 ),Infection without Glucocorticoid(n =29) and Infection with Glucocorticoid(n=30)Group were devided according to the type of disease and therapies.After being treated for 0,3,7 d,respectively venous blood samples of patients were collected to determine the concentration of CRP,WBC count,leukocyte classification and NAP score.CRP was used as the Judgment index for the degree of infection,the changes of other indexes were compared.Results Compared with the Infection without Glucocorticoid Group,the CRP concentration of the Infection with Glu-cocorcoid Group was becoming lower(P >0.05).The Non-infection with Glucocorticoid Group applied glucocorticoid for 3 days and 7 days respectively,the count of neutrophilic granulocyte and NAP score increased significantly compared with that before glucocor-ticoid application(P <0.01)while the count of lymphocyte reduced(P <0.05)and no significant change in the count of eosnophils (P >0.05).Conclusion The increase of WBC after glucocorticoid can not be identified whether it is the adverse reaction of glu-cocorticoid or aggravated infection by using classification of leukocyte,the increasing amount of WBC and NAP score.