Objective To explore the clinical eficacy of umbilical cord blood stem cell transplantation in treatment of decompensated cirrhosis.Methods Thirty patients with decompensated cirrhosis were given umbilical cord blood stem cell transplantation (treatment group) and 30 patients with decompensated cirrhosis were given traditional treatment (control group).Liver function and blood coagulation function was tested after 4,8 weeks treatment respectively,and adverse effects were recorded at the same time.Results After 8 weeks treatment,total bilirubin,albumin and prothrombin time in treatment group was improved compared with that before treatment[(71.3 ± 37.8) μ mol/L vs.(107.3 ± 53.2) μ mol/L,(30.1 ± 4.9) g/L vs.(27.5 ± 5.1) g/L,(15.0 ± 2.9) s vs.(16.7 ± 3.9) s],and there was significant difference (P ＜ 0.05).There was no significant difference in the index before and after treatment in control group (P＞ 0.05).No obvious adverse reactions were observed in the process of umbilical cord blood stem cell transplantation.Conclusion Umbilical cord blood stem cell transplantation is safe and effective in treatment of deeompensated cirrhosis.

Objective To study the effects of overexpression of HRAD17 on radiation-induced apoptosis of A549 cells.Methods Transfection of FLAC-tagged HRAD17 expression plasmid into human A549 cells was carried out and flow cytometry was employed tO observe the apoptotic effect.In addition,phosphorylation of p53 Ser46 was detected by Westem blot and the transcription of p53 AIP1 was measured by RT-PCR.Results Overexpression of HRAD17 gene significantly increased radiation-induced apoptosis of A549 cells.Further.Ser46 phosphorylation of P53 protein was increased and transcription of p53 AIP1 gene was elevated in cells overexpressing HRAD17.Conclusions HRAD17 protein can sensitize A549 cells to radiation-induced apoptosis in part by increasing p53 AIP1 expression.

Objective To explore the diagnostic and therapeutic effects of laparoscopy for early atypical tubal pregnancy.Methods Laparoscopy was conducted for diagnosing and treating 38 cases of early or atypical tubal pregnancy.For patients with blue and purple pregnant swellings seen clearly in the fallopian tubes,or those with one side of fallopian tube locally swollen and purple without obvious pregnant swellings observed,combination of fallopian tubes incision to take out embryo and salpingorrhaphy was performed.For those cases with normal fallopian tubes on both sides in appearance and without current desire of pregnancy,diagnostic uterine curettage was applied.After the diagnosis of tubal pregnancy was confirmed,30 mg of MTX was injected into ampulla of both sides.For patients with demand of reproduction,diagnostic uterine curettage was not performed.Results Five cases were misdiagnosed before operation,the misdiagnosis rate was 13%.Three cases were misdiagnosed by laparoscopy,and the rate was 8%.Fallopian tubes incision for embryo-taking under laparoscope combined with salpingorrhaphy were applied to 30 cases.Four cases were treated conservatively with injecting 30 mg of MTX into the fallopian tubes.The success rate was 100%.Blood ?-hCG was back to the normal level(4.2?3.1)days after surgery.Conclusions Laparoscopy is the optimal technique for the diagnosis and treatment of early atypical tubal pregnancy.

Objective To evaluate the clinical significance of measurement of urinary leukotrience E4(LTE4) in patients with asthma.Methods Urinary leukotriene E4 in 28 patients with asthma who experienced acute attack period and asymptomatic period and 18 controls was measured by ACETM competitive enzyme immunoassay and expressed as pg/mg creatinine.In addition,forced expiratory volume in one second was investigated in the patient in acute attack period.Results Urinary LTE4 in acute attack period was significantly higher than that in asymptomatic period(P

Objective To understand the current situation of hospital infection management in some hospitals in China and explore its development tendency. Methods Surveys were made by means of questionnaires on hospital infection management in 16 hospitals of various tiers in Beijing, Shanghai and Guangzhou and the results were compared with those of the two nation-wide surveys conducted respectively in 1994 and 1999. At the same time a dynamic assessment was made of hand hygiene implementation. Results The infection management systems in large and medium-sized hospitals in China were becoming increasingly perfect and a fairly high level was reached in function construction, team structure and expertise of hospital infection management departments. However, there were still many problems. 60% of medical staff washed their hands after patient-contact while only 35% did so before patient-contact and after item contact. Conclusion Hospital infection management in China has reached an important stage of accelerated development with substantial accumulation and more solid foundation. However, as far as development tendency is concerned, we should introduce technical indexes for quality control, put more emphasis on process-evaluation rather than result-evaluation in quality control management, stress the guiding role of evidence-based medicine in hospital management and promote a benign interaction between the hospital infection management and medical treatment process.

Objective To evaluate hand hygiene compliance (HHC) after patient contact and the influence of different factors on HHC in China. Methods HHC by different levels of medical workers from different departments were observed on 8 tertiary hospitals in Beijing, Shanghai and Guangzhou. Results The average HHC was 56. 5%( 61. 0% by the doctors and 53. 8% by the nurses). HHC was 52. 8% in the presence of gloves and 82. 0% in the absence of gloves; the difference was statistically significant. When alcohol-based hand rub or soap (hand sanitizer) was provided, HHC was respectively 58. 7% and 61. 3%; when they were not provided, it was respectively 51. 2% and 48. 4% (P

Objective To assess the value of the myocardial segment number of color change in the bull's eyes of three-dimensional speckle tracking echocardiography (3D-STE)in the detection of multi-vessel coronary stenosis at the resting state.Methods A total of 125 consecutive patients were enrolled and divided into the following three groups according to the coronary angiography(CAG):multi-vessel coronary stenosis group (n=48),single-vessel stenosis group(n =34)and control group (n =43).All patients underwent two-dimensional (2D)and three-dimensional echocardiography (3D)and three-dimensional speckle tracking echocardiography (STE).The bull's eyes of left ventricular 17 myocardial segments including longitudinal strain(LS),circumferential strain (CS),area strain(AS)and radial strain(RS)were acquired by 3D-STE.The homogeneous colors were regarded as normal wall motion myocardial segment (LS,CS,AS is a uniform red,RS for uniform blue ),the colors were uneven or shallower,or changes were regarded as abnormal wall motion myocardial segment.The myocardial segment number of color change in the bull's eyes can be calculated.Receiver operating characteristic (ROC ) curves were computed to determine optimal strain cutoff values to predict multi-vessel coronary stenosis.Results The myocardial segments of abnormal wall motion in the bull's eyes (LS,CS,AS,and RS)were significantly increased compared with the control group(P <0.001).In particular,the multi-vessel coronary stenosis group were dramatically increased than the single-vessel coronary stenosis group.Meanwhile,these parameters were higher in patients with multi-vessel coronary stenosis than in those with single-vessel coronary stenosis (P <0.05).ROC analysis demonstrated areas under the curve of 0.84 for 3D GLS,0.89 for 3D GCS,0.95 for 3D GAS,and 0.89 for 3D GRS.An optimal cutoff value of magnitude,sensitivity and specificity were LS,12,89.6%,76.7%;CS,11,89.6%,74.4%;AS,12,91.7%,88.4%;RS,13,81.2%,86.0%,respectively. Conclusions The myocardial segment number of color change in the bull's eyes by 3D-STE is useful to detect multi-vessel coronary stenosis,where in GAS are more valuable indicators with higher sensitivity and specificity.

Objective Study on the diagnosis value of expression of serum macrophage inhibitory factor 1 (MIC-1) combined with alpha-fetoprotein isoforms 3 (AFP-L3) in primary liver cancer detected by enzyme linked immunosorbent assay (ELISA).Methods MIC-1 and AFP-L3 concentrations were detected by ELISA from selected 116 patients of primary liver cancer.Results were compared both in combined and sigle detection.Results In primary liver cancer group AFP-L3 concentration [(127.12±51.43) ngmml] was significantly higher than that in normal control group [(27.11±7.26) ng/ml,P ＜ 0.001].With AFP-L3 ＞ 38.0 ng/ml as the critical value,the sensitivity was 85.34 ％ (99/116),specificity was 88.33 ％ (53/60) and the diagnostic accuracy was 86.36 ％ (152/176).In primary liver cancer group MIC-1 concentration [(3140.43±1138.23) pg/ml]was significantly higher than that in normal the control group [(701.88±302.34) pg/ml,P ＜ 0.001],the sensitivity was 91.38 ％ (106/116),specificity was 85.00 ％ (51/60),the diagnostic accuracy was 89.20 ％(157/176).The two combined detection sensitivity was 83.62 ％ (97/116),specificity was 91.67 ％ (55/60),diagnostic accuracy was 86.36 ％ (152/176).Conclusion MIC-1 combined with AFP-L3 concentration detection can improve the specificity of the diagnosis of primary liver cancer,which has certain clinical value.

Simple sequence repeat (SSR) was used to investigate the genetic diversity and structure of Dendrobium officinale. A total of 15 primer pairs with stable and repeatable polymorphism were screened out from 60 SSR primer pairs developed by the method of microsatellite enrichment by magnetic beads. Forty-eight samples of Dendrobium officinale were analyzed in genetic polymorphism. These loci were polymorphic and displayed 3 to 9 alleles per locus with a mean number of 6.1. The observed and expected heterozygosities ranged from 0.60 to 0.85 and from 0.49 to 0.85 respectively. The polymorphic information content (PIC) of each SSR locus varied from 0.437 to 0.829 with an average of 0.702. Fifteen primer pairs were used in Dendrobium cross-species amplification and totally 13 primer pairs were proved to have the transferability in D. officinale related species. In addition, 500 tissue culture plantlets of D. officinale were tested for purity identification by means of PCR amplification with four SSR primer pairs. The results showed that SSR technique is a feasible, simple and inexpensive method for determining adulterants in germplasm identification.

Objective To explore the effect of experimental design in the experimental teaching of fundamental nursing. Methods 168 and 199 baccalaureate nursing students were in the control and experimental group respectively, the control group received traditional experimental teaching method, and the experimental group must complete the experimental design before entering the lab. The teaching effect between the two groups were compared. Results The mean operation score of the experimental group was significantly higher than the control group, the proportion of those students who reflected their teaching method could enhance creative ability, problem-solving ability and practical ability was higher in the experimental group, 100% teachers reflected experimental design could improve operation level, strengthen theoretical knowledge, animate teaching atmosphere. Conclusions Experimental design could arouse students' enthusiasm and initiative to study, advocate them to think and ask.