Objective To investigate the value of the combined test of five tumor markers for gastric cancer diagnosis.Methods The electrochemical luminescence analyzer was used to measure the serum concentrations of CEA,CA125,CA199,CA724 and AFP in 127 gastric patients and 186 controls,and calculated the sensitivity and specificity.Results The concentrations of CEA (52.9 ±25.5)ng/mL,CA125 (54.2 ±40.6)U /mL,CA199 (42.4 ± 28.8)U /mL,CA724 (9.3 ±6.6)U /mL and AFP (22.6 ±11.4)ng/mL in the gastric cancer group were significantly higher than those in the controls,and the differences were statistically significant (t =6.006,7.118,6.033,6.683, 5.362,all P <0.05 ).The sensitivity in gastric cancer diagnosis with the combined test of five tumor markers (88.2%)was higher than the test of CEA (63.8%),CA125 (59.1%),CA199 (41.7%),CA724 (37.0%)and AFP (46.5%)alone,and the differences were statistically significant (χ2 =20.733,27.754,60.209,71.046, 50.270,all P <0.05).Moreover,the specificity in gastric cancer diagnosis with the combined test of five tumor markers (90.3%)was higher than the test of CA125 (79.3%),while lower than the test of CA724 (97.3%),and the differences were statistically significant (χ2 =9.137,7.832,all P <0.05).Conclusion The combined test of five tumor markers (CEA,CA125,CA199,CA724 and AFP)could increase the rate of gastric cancer diagnosis.

Objective To observe the clinical efficacy of needling the three trembling points plus rehabilitation and taking levodopaand benserazide hydrochlo-ride.Method Ninety-three patients with early Parkinson's disease were randomized into group A of 32 cases, group B of 30 cases, and group C of 31 cases. Group A was intervened by needling the three trembling points plus rehabilitation and taking levodopaand benserazide hydrochlo-ride, group B by rehabilitation and taking levodopaand benserazide hydrochlo-ride, while group C by taking levodopaand benserazide hydrochlo-ride. UPDRSⅢ scores and Berg balance scale (BBS) scores were observed.Result After intervention, UPDRSⅢ scores and BBS scores were significantly changed in all groups (P<0.05). UPDRSⅢ scores of group A were markedly different form that of group B and C (P<0.05). BBS scores of group A and B were markedly different form that of group C (P<0.05).Conclusion Needling the three trembling points plus rehabilitation and taking levodopaand benserazide hydrochlo-ride can improve motor function and equilibrium function in the early Parkinson's disease patients.

The effects of intramuscular or intracerebroventricular injection of penta-gastrin(PG) on the action potential amplitude (APA) of the myocardial cells and the heart rate(HR) were studied. The results were as follows:1 ) Injection of 10?g/10?l of PG into one of the lateral ventricles of the ratfailed to produce any effect on APA or HR. When the dosage of PG was doubled(20?g/10?l), then HR could be slowed down significantly (P

Dementia is a syndrome mainly characterized by acquired cognitive impairment, which is mainly manifested by the decrease of cognitive functions such as understanding, orientation, and visuospatial ability. Due to different intervention methods for different types of dementia, differential diagnosis is extremely important. Positron emission tomography (PET) can reflect the changes of brain function from multiple angles through different tracers, providing imaging basis for the differential diagnosis of dementia. This article reviews the characteristics of PET in patients with different types of dementia in order to provide ideas for the differential diagnosis of patients with different types of dementia.

Objective:To analyze the difference in immune microenvironment between primary tumor tissues and metastatic tumor tissues of metastatic colorectal cancer, and to screen specific immune-related differentially expressed genes (DEG) related to prognosis of metastatic colorectal cancer via bioinformatics methods.Methods:The GSE131418 microarray dataset of colorectal cancer and metastases was downloaded from gene expression omnibus (GEO) database, including 517 samples from the MCC cohort and 618 samples from the Consortium cohort in Moffitt Cancer Center. Immune-related gene sets were downloaded from immunology database and analysis portal IMMPORT, including 2 483 immune-related genes. A total of 695 cases of RNA sequencing data and 627 cases of clinical information of colorectal cancer tumors and adjacent tissues were downloaded from Cancer Genome Atlas (TCGA) data. The stroma cell score, immune cell score and stromal immune total score of metastatic tumor tissues and primary tumor tissues were calculated by using ESTIMATE algorithm, and 22 kinds of immune cell infiltration in primary tumor and metastatic tumor tissues of colorectal cancer were compared and analyzed by using CIBERSORT deconvolution algorithm. Immune-related DEG were screened to make Kyoto Encyclopedia of Genes and Gnomes (KEGG) signaling pathway enrichment analysis. The patients were divided into high and low expression groups according to the median expression levels of immune-related DEG. The Kaplan-Meier method and Cox regression risk model were used to analyze immune-related DEG, and the genes significantly related to prognosis in the results of the two methods were screened (all P < 0.01), and multivariate analysis was performed by using Cox regression method. The expression differences of each gene in tumor tissues, adjacent tissues, primary tumor tissues and metastatic tissues in GSE131418 data sets of TCGA database and GEO database were compared, and survival analysis was also performed. Results:The stroma cell score, immune cell score and stromal immune total score of colorectal cancer metastatic tissues were lower than those of primary tumor tissues (all P < 0.001). Compared with primary tumor tissues, the proportion of activated natural killer (NK) cells, monocytes, CD8 + T cells, T cells, activated dendritic cells in metastatic colorectal cancer tissues was increased, while the proportion of inactive mast cells, inactive dendritic cells, inactive NK cells, activated memory CD4 + T cells, M1 macrophages, and neutrophils was decreased. There were 289 immune-related DEG in metastatic tissues and primary tumor tissues of metastatic colorectal cancer, including 101 up-regulated genes and 188 down-regulated genes. KEGG signaling pathway enrichment analysis showed that in the immune microenvironment of metastatic tissues in metastatic colorectal cancer, vascular endothelial growth factor (VEGF) signaling pathway, programmed death ligand 1 (PD-L1) expression and programmed death 1 (PD-1) checkpoint pathway, T helper cell (Th) 1, Th2 and Th17 cell differentiation, NF-kappa B signaling pathway, interleukin 17 (IL-17) signaling pathway, chemokine signaling pathway, T cell receptor signaling pathway, MAPK signaling pathway, and NK cell-mediated cytotoxicity pathways enrichment were detected. Immune-related DEG related to prognosis including ANGPTL5, FPR1, HSPA8, NR2E3, PSMD2, PSMD8 and SBDS were screened out. Cox regression multivariate analysis showed that immune-related DEG ANGPTL5 ( HR = 2.69, 95% CI 1.22-5.92, P < 0.05), HSPA8 ( HR = 0.57, 95% CI 0.33-0.97, P < 0.05), and SBDS ( HR = 2.23, 95% CI 1.18-4.21, P < 0.05) were independent prognostic factors for metastatic colorectal cancer. The expression of ANGPTL5 in tumor tissues was lower than that in normal tissues, and the expression of ANGPTL5 in metastatic tissues was higher than that in primary tumor tissues. Patients with high expression of ANGPTL5 in tumor tissues had worse prognosis. The expression of HSPA8 in tumor tissues was higher than that in normal tissues, and the expression of HSPA8 in metastatic tissues was lower than that in primary tumor tissues. Patients with high expression of HSPA8 in tumor tissues had a better prognosis. The expression of SBDS in tumor tissues was lower than that in normal tissues, and the expression of SBDS in metastatic tissues was lower than that in primary tumor tissues. Patients with high expression of SBDS in tumor tissues had worse prognosis. Conclusions:Immune microenvironment of metastatic colorectal cancer is quite different from that of primary tumor. The degree of immune cell infiltration is reduced and the whole is immunosuppressed. The specific immune-related DEG related to prognosis of metastatic colorectal cancer may be new therapeutic targets of metastatic colorectal cancer.

Objective To evaluate the effects of mindfulness training on diabetes-related distress and quality of life in patients with type 2 diabetes mellitus, so as to provide evidence for nursing interventions. Methods A total of 90 inpatients with type 2 diabetes mellitus were randomly assigned to a control group and an observation group of 45 cases. The control group received routine health education, while the observation group received mindfulness training by the mindfulness training team composed of seven members including psychological counselors and nurse educators. Both groups were followed up for 6 months, and the scores of diabetes-related distress and quality of life were measured before and 6 months after the intervention. t-test was adopted to compare the difference between the two groups. Results After the intervention, the total score and scores of each dimension of diabetes-related distress in the observation group were (1.81±0.40), (1.99±0.45), (1.45±0.20), (2.01±0.27), and (1.51±0.21) respectively. Correspondingly, the scores of the control group were (2.07±0.24), (2.21±0.40), (1.61±0.19), (2.57±0.25) and (2.20±0.23), which were all higher than the observation group, with statistical differences (t=3.54, 2.32, 3.69, 9.68, 14.11;P<0.05). The scores of quality of life in disease dimension, physiological dimension, psychological dimension, social dimension and satisfaction dimension were (28.41±5.40), (21.05±4.16), (21.08±5.16), (19.04±3.30) and (22.84±3.79) in the observation group, which were all lower than those in the control group respectively [(36.08±5.20), (23.80±5.00), (24.11±5.22), (21.34±3.47), (24.64±3.50)], the differences were statistically significant (t=6.51, 2.69, 2.63, 3.06, 2.22;P< 0.05). Conclusions Mindfulness training can reduce the degree of diabetes-related distress and improve the quality of life in patients with type 2 diabetes mellitus.

Objective To investigate the effect of diet intervention based on action research method on dietary knowledge and blood glucose level in diabetic patients with stroke.Methods From April to June 2016, a total of 64 patients with diabetes mellitus combined with stroke were selected and divided into control group and intervention group by random number table method. The patients in the control group were given routine health education. In the intervention group, the action research method based diet intervention was implemented on the basis of the control group, including plan, action, observation, and reflection, 4 steps in total. Diet cognitive level, fasting blood glucose, 2 hours postprandial blood glucose were compared between two groups before and after the intervention.Results There were no significant differences in diet cognitive level, fasting blood glucose, 2 hours postprandial blood glucose between the two groups before the intervention (P>0.05). After the intervention, patients' diet cognitive level, fasting blood glucose, 2 hours postprandial blood glucose in the intervention group were all better than those in the control group, and the differences were statistically significant (P<0.05).Conclusions Dietary intervention based on action research method can improve the cognitive level of diet management and improve the blood glucose level of diabetic patients with stroke.

Objective To explore the effects of telephone follow-up on patients with sub-clinical hypothyroidism. Methods Of 120 patients who were diagnosed as sub-clinical hypothyroidism between January, 2014 and June,2014, 60 were included in control group and 60 were assigned in observation group by a random number table. Patients in control group received routine check-up and treatment protocols according to their condition, in contrast, patients in observation group had extra telephone follow-up. Patients′symptoms, the degree of goiter, thyroid ultrasound examination, thyroid function and thyroid peroxidase antibody (TPOAb), and other indicators were compared in the two groups after 12 weeks intervention. Results After the intervention, both groups had an improvement in slow reactions, chills, skin changes, body mass increase, etc. After invention, thyroid stimulating hormone and TPOAb in observation group were statistically different from those in control group [(1. 955 ± 1. 633) vs (2. 875 ± 3. 025) mIU/L, (204. 500 ± 825. 500) vs (438. 500 ± 1065. 250) IU/ml] (Z=2. 539,1. 451;P<0. 05). Patients with thyroid enlargementⅢdegrees reduced from 29 cases to 14 cases in observation group, which was better than that in control group (χ2 =2. 199, P<0. 05). Thyroid ultrasound results were similar between the two groups (χ2 =0. 043, P >0. 05). Conclusions Telephone follow-up intervention is helpful to improve the symptoms of patients with sub-clinical hypothyroidism, alleviate goiter and promote thyroid function back to normal.

Objective To observe the influences of nursing intervention in diabetics,guided by protection motivation theory (PMT),of their protection motivation of blood sugar monitoring,frequency of monitoring blood sugar and glycosylated hemoglobin,and level of their blood sugar and glycosylated hemoglobin.Methods A total of 100 diabetics eligible for this research were randomly divided into intervention group and control group,each with 50 cases.The patients in the control group were given routine nursing and the patients in the intervention group were given a further 6-month protection motivation theory-based nursing intervention.Changes in protection motivation of blood sugar monitoring,in frequency of monitoring blood sugar and glycosylated hemoglobin,and in index of blood sugar and glycosylated hemoglobin were compared.Results Before the intervention,comparison between scores in each dimension of protection motivation in blood sugar monitoring,frequency of monitoring blood sugar and glycosylated hemoglobin,and values of blood sugar and glycosylated hemoglobin between the two groups were not statistically significant (P ＞ 0.05).After the 6-month intervention in the intervention group,the susceptibility score was (6.530 ±0.952);the self-efficacy score was (9.462 ±0.617);blood glucose monitoring frequency was (24.126 ±2.712) times;glycosylated hemoglobin monitoring frequency was (2.080 ±0.868) times;fast blood sugar was (7.080 ± 0.824) mmol/L;glycosylated hemoglobin was (6.823 ± 0.158) ％;the scores and values in the control group were (3.820 ± 0.723),(6.729 ± 1.341),(18.528 ± 2.831) times,(1.812 ± 0.778) times,(8.488 ± 1.592) mmol/L,and (9.853 ± 0.899) ％,with significant differences (t =16.742,8.435,14.626,12.489,-15.537,3.395;P ＜ 0.05).Conclusions Nursing interventions guided by protection motivation theory can improve diabetics' protection motivation and promote behavior of blood sugar monitoring and their metabolic index up to standard.

Objective To observe clinical nurses′knowledge, attitude, and practice of evidence-based practice (EBP), and to explore its potential influencing factors, so as to provide targeted knowledge training for nurses.Methods The nurses′ information form and evidence-based practice questionnaire ( EBPQ) were used in this survey. Totally 140 nurses from a hospital in Beijing from June to July 2015 participated in this survey. Results The average score of practice, attitude, and knowledge on EBP were (22.94±7.32), (21.69±6.47) and (57.39±15.31). Statistically significant correlations were found among different nurses′ age, length of employment, educational background, job tittle and nurses′ practice, attitude, and knowledge score on EBP ( P<0.05) . Multivariate linear regression showed that the EBP of nurses was mainly decided by their knowledge level, attitude level, amount of literature reading, and endorsement related to EBP.Conclusions The status of nurses′knowledge and practice level related to EBP was low, and the attitude level was relatively positive. Knowledge training program should be established according to nurses′ age, length of employment, educational background, and job tittle, in order to raise the level of their knowledge, attitude and behavior on EBP. As well as the amount of literature reading and endorsement related to EBP are also primarily factors that influence the practice level.