Objective To summarize the experience of diagnosis and treatment of gastric non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue.Methods Twenty-seven patients,proved by pathology,were included in the study.Results Among clinical presentations,the upper abdominal pain,intestinal bleeding,and weight loss were common.Only 1 case was diagnosed definitely from 18 cases with the examination of X-ray barium meal,84.6%(24 of 26 cases)were miss-diagnosed under gastroscopy.All cases underwent operation,among them 25 performed a radical operation.Twenty-four patients were followed up.Conclusion The multiple biopsy sampling from submueosal layer via gastroscope may improve diagnostic rate on gastric non-Hodgkin's lymphoma of mucosa-associated lymphoid tissue.Operative removal of the tumor should be the first choice of treatment.Additional chemotherapy after the surgery increases survival rate.

Objective To investigate the diagnosis and treatment of acute acalculous gangrenous cholecystitis (AAGC). Methods The clinical data of 46 patients with AAGC treated in our hospital from 1990 to 2006 were retrospocfively analyzed. Results All patients with AAGC were surgically treated when the final diagnosis was confirmed. Of the 46 patients, 32 underwent cholecystectomy, 14 partial cholecystectomy and 5 cholecystectomy, exploration of common bile duct and T-tube drainage. Tow patients died after operation due to toxic shock and multiple organ failure. Conclusions B-mode ultrasonography, laboratory examination, symptoms and clinical signs are the main methods for diagnosis of AAGC. Early diagnosis, careful preoperative preparation and surgical therapy are important to raise curative rate of the disease.

Objective To explore the clinical features and treatment of the cystic change of thyroid canc-er. Methods A retrospective analysis was made based on the clinical data of 25 patients with cystic change of thyroid cancer from 1994 to 2008. Results lacking the specificity in clinical diagnose, the di-agnosis accuracy of the disease before operation was very low and the misdiagnosis rate was very high. A-mong 25 cases, only one was accurately diagnosed before operation, 3 were reoperated after the accurate di-agnosis by using intra-operative frozen section examination. Conclusions Clinical physicians should raise their awareness to such a disease. To improve the accurate diagnosis of the misdiagnosis of the cystic degen-eration of thyroid cancer, clinical physicians should integrate the examinations including B-ultrasound, preop-erative fine-needle aspiration biopsy,and intra-operative frozen pathological section.

Objective To explore the diagnostic and therapeutic effects of laparoscopy for early atypical tubal pregnancy.Methods Laparoscopy was conducted for diagnosing and treating 38 cases of early or atypical tubal pregnancy.For patients with blue and purple pregnant swellings seen clearly in the fallopian tubes,or those with one side of fallopian tube locally swollen and purple without obvious pregnant swellings observed,combination of fallopian tubes incision to take out embryo and salpingorrhaphy was performed.For those cases with normal fallopian tubes on both sides in appearance and without current desire of pregnancy,diagnostic uterine curettage was applied.After the diagnosis of tubal pregnancy was confirmed,30 mg of MTX was injected into ampulla of both sides.For patients with demand of reproduction,diagnostic uterine curettage was not performed.Results Five cases were misdiagnosed before operation,the misdiagnosis rate was 13%.Three cases were misdiagnosed by laparoscopy,and the rate was 8%.Fallopian tubes incision for embryo-taking under laparoscope combined with salpingorrhaphy were applied to 30 cases.Four cases were treated conservatively with injecting 30 mg of MTX into the fallopian tubes.The success rate was 100%.Blood ?-hCG was back to the normal level(4.2?3.1)days after surgery.Conclusions Laparoscopy is the optimal technique for the diagnosis and treatment of early atypical tubal pregnancy.

A microbial consortium of Trichoderma reesei AS3 3711, Aspergillus niger AS3 316 and Saccharomyces cerevisiae AS2 399 was constructed to decomposed rice chaff on the basis of the characters of each microorganism and the mechanism of cellulases In this experiment, rice chaff was pretreated with NaOH before fermentation so that the lignin structure of rice chaff was degraded and hemi cellulose was dissolved partly, which remove the protection of lignin and hemi cellulose on cellulose and demolish its special crystal structure After pretreatment, rice chaff can be degraded more easily with the microbial consortium The optimal technical paths and technological methods were achieved for intenerating rice chaff with the microbial consortium perfectly through orthogonal experiment According to the technological methods, some experiments were done at 30℃ with pH 4 5 It was found that the highest filter paper enzyme activity (FPA) was 5 64U/g and the ratio of cellulose degradation (RCD) was 28 05%

Objective To find out the bacterial species in the vagina of postpartum women and the possible influencing factors on colonization. Methods From Jun. 2007 to Oct. 2007, 560 postparmm women from 7 hospitals in China were enrolled. Questionnaire survey, gynecological examination and Nngent score of vaginal smear and microbial spectrum study of the vaginal flora were completed. Results (1 ) According to the Nngent score, 48 out of the 560 women were normal (8.6%), 337 at the borderline (60. 2% ) and 175 (31.2%) were complicated with bacterial vaginosis (BV). Among the 560 women, Bacterium lacticum were identified in 74 cases (13. 2% ), but not in the rest 486 cases (86. 8% ). Gardnerella and bacteroids were detected in 322 women (57. 5% ) and small flectobacillus in 214 women (38. 2% ) out of the 560 subjects. (2) Influencing factors on vaginal microflora: among the 266 women who had normal vaginal delivery, 23 (9. 4% ) showed normal vaginal microflora, 148 (55.6% ) at borderline and BV was diagnosed in 93 women (35.0%). The corresponding figures among the 294 women who underwent cesarean section were 23 (7. 8% ), 189 (64. 3% ) and 82(27.9% ), respectively. However, the incidence of BV had no statistical difference between these two groups (P = 0. 204). In the 233 women who received episiotomy, 22 (9. 4% ) showed normal vaginal microflora, 135 (57.9%) at borderline and 76 presented with BV (32. 6% ), the corresponding figures among the 327 women without episiotomy were 26 (8. 0% ), 202 (61.8%) and 99 (30. 2% ), respectively. The incidence of BV did not show any statistical difference between the above two groups (P = 0. 790 ). (3 ) Prenatal vaginitis were reported in 46 women, among which 5 (10.9%) with normal vaginal flora, 26 (56.5%) at borderline and 15 (32.6%) with BV, and again in the 514 women without prenatal vaginits, the above figures changed to 43 (8.4%) , 311 (60. 5% ) and 160 (31.1%). No significant difference was found in the incidence of BV between the two groups (P =0. 962). The rate of BV in women without sex, with sex occasionally and with sex frequently during pregnancy was 27.5% (78/284), 35.6% (96/270) and 1/6, respectively (P = O. 185), and the numbers in women who had breast-feeding, bottle feeding and mixed feeding were 31.0% (67/216), 39.3% (35/89) and 28.6% (73/255), respectively (P=0.573). Conclusions The amount of Lactobacillus in vagina of postpartum women is greatly reduced leading to dysbacteria. The incidence of BV is not affected by vaginal delivery, episiotomy, vaginitis, prenatal intercourse and the way of feeding, but is higher in postpartum women.

Objective To investigate the clinical significance of the levels of D-dimer and hs-CRP in the elderly patients with coronary disease. Methods One hundred and twenty-two patients with coronary disease were divided into four groups according to their clinical manifestations, there were old myocardial infarction group ( OMI, n =27 ) , stable angina pectoris group ( SAP, n = 29), acute coronary syndromes group ( ACS, n = 32) and old myocardial infarction with acute cardiovascular syndrome group (OMI + ACS,n =34). The control group included the patients without coronary disease (n =30). The patients who had infection, malignant tumors, liver and kidney dysfunction, cerebral infarction, venous embolism in lower extremities and pulmonary embolism were not included, and the patients enrolled should not be on medication of antiplatelet drugs, anticoagulants and antibiotics within the last two weeks. The levels of D-dimer and hs-CRP in all groups were determined by immunoturbidimetric assay and immune scattering assay respectively. Results In each comparison between two groups, the level of D-dimer has no significant difference between the OMI and the SAP ( P > 0. 05 ) , ACS and the OMI + ACS ( P > 0. 05 ) ; but significant difference between the OMI and the control groups ( P < 0. 05) and more significant difference between the other groups were found (P < 0. 01). As for the level of hs-CRP, there was no significant difference between the OMI and the SAP groups (P > 0. 05) or between the ACS and the OMI + ACS groups (P > 0. 05); but significant difference between the other groups (P < 0. 01). the positive relationship between the levels of D-dimer and hs-CRP have in coronary disease in the elderly patients was also found(r = 0. 81 ,P <0.01). Conclusions D-dimer and hs-CRP can be used as the clinical makers to predict the occurrence of the coronary disease in the elderly patients. D-dimer and hs-CRP can be used as the clinical makers to determine the stability of cardiovascular atherosclerotic plaque and the risk of acute cardiovascular syndrome occurrence. They can also be used as the clinical makers to predict the recurrence of the acute cardiovascular syndrome in the patients with old myocardial infarction. However, they can not be used to determine whether the patients with acute cardiovascular syndrome had old myocardial infarction already or not. The levels of D - dimer and hs - CRP are closely related in coronary disease in the elderly patients.

Objective Discussion leukoreduction of red blood cells suspended in two different storage bag placement and he-molysis rate impact on the supernatant free hemoglobin (FHb),to ensure the clinical transfusion is safe and effective.Meth-ods Selected 20 donors to sample 400 ml whole blood per person to make leukodepleted red blood cells,which were evenly divided into 10 bags.The 10 bags were randomly divided into two groups,one to the upright position,one group of horizon-tal.The two groups were stored under the same conditions.Respectively,in the 7,14,21,28,35 day,randomly removed one storage bag from each group,FHb and red blood cell hemolysis rate were measured and analyzed statistically.Results FHb and hemolysis rate results stored in the first 21 days of testing,uprightgroup were (217.310±48.477)mg/L and (0.250± 0.056)%,respectively horizontal group (173.972±39.027)mg/L and (0.189±0.045)%,the results set upright than hori-zontal group,the results were statistically(t=3.114,P =0.003<0.05 and t=3.798,P =0.001<0.05),the difference was statistically significant.Conclusion In the blood storage period,storage bags can be placed horizontally to reduce the de-struction of red blood cells,blood storage is more favorable.

A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reac-tion condition contained 1.5μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was validated by several intra-and inter-day assays. The production transitions of m/z 147.0/59.1 and m/z 154.0/59.1 were used to detect and quantify mevalonolactone (MVAL) and MVAL-D7, respectively. The accuracy and precision of the method were evaluated over the concentration range of 0.005–1.000μg/mL for MVAL and 0.010–0.500μg/mL for lovastatin acid in three validation batch runs. The lower limit of quantitation was found to be 0.005μg/mL for MVAL and 0.010μg/mL for lovastatin acid. Intra-day and inter-day precision ranged from 0.95%to 2.39%and 2.26%to 3.38%for MVAL, 1.46%to 3.89%and 0.57% to 5.10% for lovastatin acid, respectively. The results showed that the active ingredients in Xuezhikang capsules were 12.2 and 14.5 mg/g, respectively. This assay method could be successfully applied to the quality control study of Xuezhikang capsule for the first time.

Objective To compare the clinical characteristics of heart failure patients with preserved ejection fraction (HFPEF) and reduced ejection fraction (HFREF).Methods A total of 171 patients with heart failure from September 2015 to November 2017 in the Second Hospital of Shanxi Medical University were retrospectively analyzed . According to left ventricular ejection fraction (EF),the patients were divided into two groups : HEPEF [EF≥45%, 84 cases,mean age (64.8 ±2.2)years old,33 males and 51 females] and HFREF (EF＜45%,87 cases,mean age (74.6 ±1.5) years old,including 61 males and 26 females).The clinical data of the two groups including general information,underlying etiology,biochemical indicators,echocardiography results,medication in detail were recorded. Results Among the 171 patients, there were 69 patients with coronary heart disease , 44 patients with dilated cardiomyopathy,21 patients with hypertensive heart disease ,10 patients with pulmonary heart disease ,15 patients with rheumatic heart disease,7 patients with degenerative valvular disease ,2 patients with congenital heart disease ,2 cases of heart failure caused by atrial fibrillation ,1 case of viral myocarditis .The patients in the HFPEF group were older [(74.6 ±1.5)years old vs.(64.8 ±2.2) years old,t＝3.598,P＝0.015],more women(60.7%vs.29.9%,χ2＝16.410,P＝0.000),lower body weight [(62.7 ±13.4) kg vs.(68.6 ±14.9) kg,t ＝－2.395,P＝0.018],less likely to have renal insufficiency(36.7%vs.53.6%,χ2＝4.670,P＝0.041),and more likely to have hypertension (54.8%vs.37.9%,χ2＝4.871,P＝0.032) and atrial fibrillation(47.6% vs.13.8%,χ2＝23.107,P＝0.000). The HFPEF group had higher admission systolic blood pressure than the HFREF group [(131.2 ±22.2)mmHg vs. (124.1 ±24.9)mmHg,t＝2.058,P＝0.041].The BNP value[(874.2 ±912.3) pg/mL vs.(835.2 ±1 490.4)pg/mL,t＝－5.011,P＝0.000],hemoglobin value[(125.5 ±24.3)g/L vs.(134.7 ±23.9)g/L,t＝－2.460,P＝0.015] in the HFPEF group were lower than those in the HFREF group .The ESR in the HFPEF group was faster than that in HFREF group[(28.0 ±25.6)mm/h vs.(16.9 ±14.9)mm/h,t＝2.486,P＝0.017].The HFPEF group had smaller left ventricular size than the HFREF group [(50.9 ±6.4)mm vs.(67.3 ±8.5)mm,t＝－11.303,P＝0.000].RAS blockers (52.4% vs.86.2%,χ2＝23.107,P ＝0.000),spironolactone (72.6% vs.88.5%,χ2＝6.926,P ＝0.011),β－blockers (57.1% vs.75.9%,χ2＝6.739,P ＝0.015),statin (38.1% vs.54%,χ2＝4.362,P＝0.046) were used more in the HFREF group,while calcium channel blockers (38.1%vs.13.8%,χ2＝13.208,P＝0.000) and warfarin (16.7%vs.5.7%,χ2＝5.159,P＝0.129) were used more in the HFPEF group.Conclusion Different clinical characteristics were found in patients with HFPEF and HFREF ..Different types of heart failure need different treatment and prevention programs .