Objective To investigate speed-thorn Ghost-point combined with three-edged needle puncture bleeding and cupping in treatment of breast hyperplasia in order to expand the scope of the treatment of Ghost-point.Methods 35 patients with breast hyperplasia were treated three courses with speed-thorn Ghost-point combined with three-edged needle puncture bleeding and cupping.The changes of breast pain and tumor size were observed before and after the treatment.Results 35 patients with breast hyperplasia were significantly improved in their symptoms of breast pain by Ridit analysis(P<0.01),and compared with those before treatment after treatment there was signifi-cant difference,and a total effective rate was 86%.Conclusion The speed-thorn Ghost-point combined with three-edged needle puncture bleeding and treatment of cupping could effectively reduce breast pain and tunlor size in pa-tients with breast hyperplasia,and the method was simple and curative effect.

Objective:The human parental brain is a discrete set of interacting brain circuits that related with child-care feelings and behaviors.The research on it can help us to understand the essence of human parent-child re-lationship formation.In order to provide materials for the research of human parental brain and related fields,the study is ready to establish a set of standardized Chinese Infant Affective Face Picture System.Methods:By using convenience sampling,a total of 360 infant facial expression pictures of 211 Chinese infants aged from 3 to 6 months were collected.After standard processing on the photos,196 unmarried and nulliparous college students re-cruited from two universities in Chongqing and Guiyang were asked to assess them.One hundred subjects assessed the emotion categories and intensity of the pictures,and other 96 subjects assessed the emotion valence,arousal and dominance of the selected pictures.Results:The Chinese Infant Affective Face Picture System,containing 117 hap-py,92 neutral,108 sad expression pictures,was established.At the same time,the corresponding average identifica-tion rate were 89%,77%,92%,the intensity were 5.83,5.15,and 6.19,the valence were 5.92,4.55 and 2.70,the arousal were 5.11,4.18,3.77,and the dominance were 5.02,4.93,and 4.91.The system could effectively stimu-late participants'emotional reactions.There were statistically significant differences in scores of the established va-lence,arousal and dominance index between the 3 kinds of expression pictures (Ps <0.01).Conclusion:The Chi-nese Infant Affective Face Picture System has higher reliability and validity.The system could be used as emotional stimulus materials for the study of parental brain or emotion researches.

OBJECTIVE:To prepare transferrin(TF)modified tetrandrine(TET)and vincristine(VCR)active targeting lipo-somes,and to optimize its formulation. METHODS:TF modified TET and VCR liposomes were prepared by ammonium sulfate gradient method. Using comprehensive score of encapsulation efficiency of TET and VCR as index,central composite design-re-sponse surface method was used to optimize and validate mole ratio of EPC/Chol,mole ratio of EPC/PEG2000-DSPE and TF mass fraction. RESULTS:The optimal formulation was that the mole ratios of EPC/Chol and EPC/PEG2000-DSPE were 1.5:1 and 20:1, TF mass fraction was 0.10%. The encapsulation efficiency of TET and VCR were 97.80% and 93.00%,respectively. The compre-hensive score was 94.44(n=3)which was close to the predicted value of 93.81. CONCLUSIONS:The optimal formulation is sta-ble and can be used for the preparation of TF modified TET and VCR liposomes.

Objective To examine the relationship of coping style,psychological capital and vocational selection anxiety for female college graduates.Methods A sample of 330 female college graduates filled out measures for the simplified coping styles questionnaire (SCSQ),Psychological Capital Questionnaire (PCQ) and vocational selection anxiety Questionnaire(VSAQ).Results Correlation analysis showed that except the self-efficacy and positive coping style collected with concerns about job prospects was not significant (r=-0.08-0.05,P＞0.05),each dimensions of PCQ and SCSQ significantly correlated with the each dimensions of VSAQ (r=-0.44--0.13 or r=0.21-0.28,P＜0.01).Regression analysis showed that negative coping style,resilience,hope and positive coping style could directly predict vocational selection anxiety.Psychological capital were the mediators between positive coping styles and vocational selection anxiety.Conclusion Coping styles and psychological capital are closely related to the female college graduates' vocational selection anxiety,using appropriate coping styles and improving mental ability can effectively reduce vocational selection anxiety.

OBJECTIVE:To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocar-dial cell of in vitro cultured heart failure model. METHODS:The myocardial cells of neonate rat were grouped into normal control group,model group,positive control group(Deslanoside injection,1×10-7 mol/L),ginsenosides Rg1 group(1×10-8 mol/L),acon-itine group (1 × 10-9 mol/L) or their compatibilities groups (1∶1,2∶1,1∶2,V/V). Except for normal control group,other groups were given 0.8%pentobarbital sodium to induce heart failure model of myocardial cells. After modeling,each group was given rele-vant medicine for 1 h,and then the activities of T-ATPase,Ca2+-Mg2+-ATPase,Na+-K+-ATPase in cells were all detected. The activi-ties of acyl carrier protein(ACP)and lactate dehydrogenase(LDH),and the contents of brain natriuretic party(BNP),TNF-α and total glycogen were measured in cell culture fluid. RESULTS:Compared with normal control group, T-ATPase and Ca2+-Mg2+-ATPase activities were decreased significantly in model group;meanwhile,Na+-K+-ATPase activity was increased signifi-cantly,and ACP,LDH activities and BNP content in cell culture fluid were increased significantly(P<0.05). Compared with mod-el group,the activities of T-ATPase in treatment groups were increased significantly,while the activity of LDH in cell culture fluid was decreased significantly;when the volume ratio of ginsenoside Rg1 to aconitine was 2∶1,protective effect was the strongest;the activities of Na+-K+-ATPase in aconitine group and compatibility groups were all decreased significantly,with statistical signifi-cance(P<0.05). The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups,but the content of to-tal glycogen in cells and the TNF-α content of in cell culture fluid had no change (P>0.05). CONCLUSIONS:Compatibility of ginsenosides Rg1 and aconitine can improve ATPase activities and membranous permeability,regulate BNP secretion and protect myocardial cell of heart failure model,especially the compatibility of ginsenosides Rg1 to aconitine of 2∶1 ratio.

Aim To establish two new rat models of visceral hypersensitivity in IBS by two chemical irritants.Methods Acetic acid or mustard was infused for six days in intestines of adult rats.After modeling,the rectal distention was performed and the thresholds of abdominal withdrawal reflex were measured.The frequency,peak value,peak-nadir value and area of the gastric and enteric electrical activity were recorded.And the contents of 5-HT in the blood serum were detected.Results Compared with the control group,the colon and rectum's sensitivity(P<0.05)and the frequency(P<0.01) of the acetic acid model were heightened.Meanwhile,the colon and rectum's sensitivity(P<0.01),the frequency(P<0.01),peak value(P<0.05),peak-nadir value(P<0.01)and area(P<0.01),and the contents of 5-HT(P<0.05)in serum of the mustard model were all changed,which indicated the increasing of sensitivity of the model.The colon and rectum's sensitivity,the gastric and enteric electrical activity and the contents of 5-HT in serum of the proving group were recovered to some extent.Conclusion The new rat model of visceral hypersensitivity in IBS is successfully set up by stimulating the intestines of adult rats with chemical substances.

Objective:To establish an UPLC method for the simultaneous determination of andrographolide sulfonate E and an-drographolide sulfonate F in andrographolide sulfonates. Methods: The sample was separated on a Shimadzu Shim-pack XR-ODSⅢcolumn (2. 0 m × 75 mm, 1. 6 μm) with acetonitrile( A) and potassium dihydrogen phosphate buffer solution( B:1. 361 g potassium dihydrogen phosphate and 1. 0g heptane-1-sodium sulfonate were dissolved in 1 000 ml water) as the mobile phase with gradient elu-tion, and the flow rate was 0. 4 ml·min-1 ,the column temperature was 30℃, and the detection wavelength was set at 225nm. Re-sults:The linear range of andrographolide sulfonate E was 4. 39-43. 88μg·ml-1(r=1. 000 0) and the average recovery was 98. 71%(RSD=1. 41%, n=6). The linear range of andrographolide sulfonate F was 4. 43-44. 32 g·ml-1(r=1. 000 0) and the average re-covery was 99. 29%(RSD=0. 76%, n=6). Conclusion:The method is reproducible, sensitive and accurate, which can be used in the quality control of andrographolide sulfonates.

This article was aimed to study the acute toxicities on intragastric administration of differentRadix Aconiti Lateralis Praeparataprocessed products to Beagle dogs. A total of 16 healthy and qualified Beagle dogs were randomly divided into the blank group,Pao-Fu-Pian(PFP) group,Pao-Tian-Xiong(PTX) group andHei-Shun-Pian(HSP) group according to the body weight. The intragastric administration of 4 g crude herb per kg was given. Before medication, 1 h, 24 h, and 3, 7, 14 days after medication, the body weight, food consumption, rectal temperature, electrocardiogram, blood routine and blood biochemistry were measured. The results showed that after medication, all dogs in three experimental groups were depressed. And there were significant differences in the electrolytes of blood. Among them, the HSP group was the most obvious one. The red blood cells, blood sugar and triglycerides of dogs in the PFP group had significant difference. The lymphocytes and blood sugar had significant difference of dogs in the PTX group. However, after the medication of HSP, the lymphocytes of the dogs were decreased significantly. It was concluded that the toxicity of three processed products followed the order of HSP > PFP > PTX.

AIM: To investigate the chromatographic fingerprints of Guangyanling Injection(Syringa oblata Lindl) by HPLC. METHODS: The separation was performed on a Water SunFire~ TM C_ 18 4.6 mm?250 mm 5 ?m analytical column with the mobile phase consisting of methanol-water as gradient eluent at the flow rate of 1 mL/min. The UV detection was set at 280 nm. RESULTS: The HPLC-UV fingerprints of Guangyanling Injection was obtained with perfect isolation. CONCLUSION: The fingerprints could be used for the control of Guangyanling Injection.

Objeoctive To compare the effects of etomidate and propofol on cerebral oxygen metabolism in patients undergoing abdominal surgery.Methods Thirty-six ASA Ⅰ or Ⅱ patients aged 30-64 yr weighing 42-73 kg undergoing abdominal surgery under general anesthesia were randomly divided into 2 groups (n=18 each):group E etomidate and group P propofol.Left radial artery was cannulated for continuous direct BP monitoring.A catheter was inserted into right internal jugular vein(LJV) and advanced cephalad until jngatar bulb for blood sampling.Both groups received midazolam 0.08 ms/ks,fentanyl 3μg/kg and vecurunium 0.1 mg/kg and in addition group P received propofol 1.5 mg/ks and group E etomidate 0.3 mg/kg respectively for induction of anesthesia.Anesthesia was maintained with propofol infusion at 4-6 mg·kg-1·h-1 in group P and etomidate infusion at 0.4-0.7 mg·kg-1·h-1 in group E and intermittent iv boluses of fentanyl and vecuronium.ECG,MAP,HR,SpO2 and PET CO2 were continuously monitored.Blood samples were taken from radial artery and IJV for blood gas analysis and lactic acid measurement before induction of anesthesia(T1),immediately after intubation (T2),30 min after skin incision (T3) and at the end of operation(T4).The rate of cerebral O2 extraction (CERO2) was calculated.Results The hemodynamic variables were within the normal range throughout the anesthesia and operation.The oxygen saturation and oxygen partial pressure of both arterial and venous blood(SaO2,SjvO2,PaO2,PjvO2) rose significantly after induction of anesthesia in both groups.There was no significant difference in arterial and venous blood lactic acid level and SaO2,SjvO2,CaO2,CjvO2,Da-jvO2 or CERO2 at all time points between the two groups.Conclusion Both etomidate and propofol combined with midazolam and fentanyl can decrease cerebral O2 metabolic rate and there is no significant difference between the two groups.