ObjectiveTo summarize and investigate the occurrence,clinical features and treatment of cesarean scar pregnancy.Methods33 cases of cesarean scar pregnancy were retrospectively analyzed.Results10 cases of vaginal massive hemorrhage after uterine curettage,9 cases of subtotal hysterectomy,1 case was cured by MTX treatment,13 cases were cured by conservative treatment of uterine curettage after chemotherapy treatment,10 cases of successful uterus-saving by excision of lesion and uterine repair.ConclusionTransvaginal sonography was an important method in diagnosis of cesarean scar pregnancy and,the key to success was to select the appropriate individual treatment according to classification by transvaginal sonography.

Astrocytes play many important roles in the nervous system.In recent years,researchers are paying attention to the roles of astrocytes in the process of stroke.This article mainly reviews the biological functions of astrocytes and the gene expression changes of astrocytes after ischemic stroke,thereby providing new potential therapeutic targets for the treatment of ischemic stroke.

Objective To investigate the association between renin-like activity (RA) and angiotensin Ⅱ (AⅡ) and the ovarian hyperstimulation syndrome (OHSS). Methods Blood samples were taken from 42 patients undergoing in vitro fertilization (IVF). According to the ovarian stimulating response, the patients were divided into 4 groups: group Ⅰ, low responders, 7 cases; group Ⅱ, moderate responders, 8 cases; group Ⅲa high responders without using albumin, 7 cases, group Ⅲb, high responders with albumin, 10 cases; group Ⅳ, severe and moderate OHSS, 10 cases. 5 patients for intrauterin insemination with natural cycle as control. Follicular fluid (FF) was collected at oocyte retrieval from 28 IVF patients (including 7 OHSS patients). Fluid from ascites and hydrothorax was obtained from 3 OHSS patients, and peritoneal fluid obtained from 5 infertility patients by laparoscopy and ascitic fluid obtained from 6 ovarian carcinoma patients served as control. RA and AⅡ levels were measured by radioimmunoassay. Results plasma RA and AⅡ levels at mid-luteal phase from OHSS patients [(19.9?19.0)?g?L -1 ?h -1 and (397.0?378.2) ng/L] were significantly higher than those from the other patients ( P

Objective To estabilish a HPLC method for the determination of Geniposide in Jigucao Capsule. Methods The column was Lichrospher-C18(5 ?m,4.6 mm?250 mm). The determination was carried out with acetonitrile-0.1% phosphoric acid(10∶90) as the mobile phase,a flow rate of 1.0 mL/min and detected at 238 nm,and temperature of the column was 35 ℃. Results The calibration curve showed good linearity in the range of 0.14～1.68 ?g,Y =1 552.15X-6.42,r=0.999 9. The mean recovery was 97.53%(RSD=1.24%,n =6). Conclusion The method is accurate,reliable,and can be used for quality control of the preparation.

Osteogenic differentiation of mesenchymal stem cells (MSCs) is an important link of bone metabolism and bone repair, the mechanism of which is usually complicated. Recent studies have found that BMP, FGF, Wnt, Hedgehog, NELL1, IGF, Notch and othersignaling molecules are involved in the regulation of osteogenic differentiation of MSCs. These signaling molecules themselves have been shown to have a strong ability to promote osteogenic differentiation. They can activate the corresponding receptors by specific ligands, thus initiating the specific signal transduction pathways in the cell, and finally promote the transcription and translation of the genes related to bone formation. Furthermore, all the signaling pathways form a complex network which regulates and influences the osteogenic differentiation ofMSCs. Further researches on the mechanism of the interaction of these signaling pathways is of great significance in promoting the development of bone tissue engineering and the treatment of bone metabolic diseases.

Objective To survey the status quo of cervical cancer and cervical precancerous lesion among married women age 35 to 55 in Taishan City,Province and to investigate precautions against cervical cancer.Methods By the request of the government,free cervical cancer screening was carried out from 2010 to 2011,26 879 women in 2010 and 23 197 women in 2011 completed the questionnaire and were checked by gynecological examination and thinprep cytologic test(TCT) voluntarily,and women screened positive by TCT accepted the colposcopy-guided biopsy.Results In 2010,2491 cases of screening positive by TCT,accoanted for 9.47％ ;665 cases of cervical intraepithelial neoplasia ( CIN ),incidence rate was 2.47％,35 cases of invasive cervical cancer ( ICC ),incidence rate was 0.13 ％.In 2011,2038 cases of screening positive by TCT,accounted for 8.78 ％ ;314 cases of cervical intraepithelial neoplasia( CIN ),incidence rate was 1.35％,16 cases of ICC,incidence rate was 0.07％.Conclusion Carrying out cervical cancer screening,could detect CIN in time,give treatment of precancerous lesions as soon as possible and prevent cervical cancer.

TrkB was designated as the functional receptor of br ain derived neurotrophic factor． In these study, we investigated the distributi on of trkB-like immunoreactivity(trkB-IR) in spinal cord of adult cats using spe cific antiserum for trkB by immunohistochemistry ABC method． The results were a s following: trkB-IR was mainly distributed in neurons of spinal cord including ventrolateral horn, intermedius are and dorsal horn． 　　 The subcellular localization of positiveproduct was mainly in cytoplasm． Our results provided morphological evidence on the distribution of trkB in spinal cord of adult cats． It indicated the role o f trkB involve in physiological function of spinal cord of adult cat．

Objective To explore the application value of high frequency probes in ultrasonic diagnosis of acute appendicitis.Methods Using high frequency probe to detect 356 patients with right lower quadrant abdominal ultrasound,and the results were analyzed.281 cases were confirmed by operation and pathology.Results 240 cases of 356 patients were detected low frequency transducer,and the detection rate was 67.4％.Application of high frequency probe were detected in 322 cases,and the detection rate was 90.4％.Pathological progress of appendicitis with appendicitis,display rates have increased,there was a significant correlation between the two.Conclusion The use of high frequency color ultrasound imaging for suspected acute appendicitis in patients has definite diagnosis and differential diagnosis.

Objective To discuss the feasibility of the lung transplantation as an effective treatment for end-stage pulmonary diseases domestically and to compare the major issues involving the practice of lung transplantation domestically and overseas.Methods After the foundation of group of lung transplantation in May 2002, lung transplantation models of porcine were set up. From September 2002 to April 2005, in the 18 cases undergoing lung transplantation, there were subjected to single-lung transplantion (SLT, 83.3%) and 3 bilateral sequential single lung transplants without CPB (DSSLT, 16.7%). Indications for SLT (n=15) included emphysema (n=9), pulmonary fibrosis (n=3), pneumosilicosis (n=1), lymphangioleiomyomatosis (n=1) and ventricular septal defect (VSD) induced Eisenmenger's syndrome (n=1); for DSSLT (n=3) bronchiectasis (n=1) and pulmonary emphysema (n=2). Among the 15 SLTs, there were 9 cases of left SLT and 6 right SLT. Among them, 2 cases shared one same donor's lung block, one case received contralateral lung transplantation―a left donor lung implanted in the recipient’s right thorax, and one case simultaneous right SLT and VSD repair. Results In hospital mortality (HM) was 3/18 ( 16.7%). Among SLTs, early death was due to severe rejection on the 30th postoperative day in one patient and acute rejection on the 15th postoperative day in other patients, and another patient died due to pulmonary vein embolism on the 36th day. There were 3 and 2 patients with the survival time longer than 1 and 2 years respectively. The median overall survival was 10 (2 to 32) months. Conclusions Our LT program shows similar results to those reported by the International Society for Heart and Lung Transplantation for developed countries. The key of successful operation depends on the establishment of group of lung transplantation and cooperation of multi-department. Ischemic-reperfusion injury, acute-rejection and infection are the major reasons of deaths shortly after the operations.

OBJECTIVE:To establish a method for the identification of Chongqing local medicinal material Heracleum hemsley-anum. METHODS:The original plant morphology,morphological characteristics,microscopic characteristics (transverse section and powder),were identified. RESULTS:H. hemsleyanum had cone shape root,brown,many-branched with special fragrance;the microscopic characteristics of taproot a transverse showed no differences with lateral root transverse section,primary xylem was di-arch,secondary phloem was more developed than secondary xylem,secondary phloem was rich in the oil chamber. Vascular tissues contained a large number of parenchyma cells. In TLC chromatrogram,the test sample showed the same color fluorescent spots with-the substance reference of osthole and columbianadin in the corresponding locations. CONCLUSIONS:The established method is simple with high accuracy,and can be used as the basis for identification of H. hemsleyanum.