Objective To investigate the roles of phosphatidylinositol 3 kinase (PI3K) and phosphorylated Akt (P-Akt) in the pathogenesis of cervical squamous cell carcinoma and condyloma acuminatum.Methods Immunohistochemistry and Western blot were used to detect the expressions of PI3K and P-Akt in tissue specimens from the lesions of 30 cases of cervical squamous cell carcinoma,30 cases of condyloma acuminatum and the prepuce of 15 normal human controls.The average optical density and gray scale values were calculated and analyzed by t test and F test respectively.Results The expressions of PI3K and P-Akt were observed in only the basal layer of the epidermis of control specimens,but in the whole epidermis of condyloma acuminatum tissue specimens.Cervical squamous cell carcinoma tissue specimens displayed a stronger expression of PI3K and P-Akt compared with the control and condyloma acuminatum tissue specimens.As immunohistochemistry revealed,the average absorbance value for PI3K and P-Akt was 0.28 ±0.05 and 0.20 ± 0.07 respectively in cervical squamous cell carcinoma tissue specimens,0.22 ± 0.04 and 0.17 ± 0.03 respectively in condyloma acuminatum tissue specimens,and 0.16 ± 0.04 and 0.10 ± 0.02 respectively in the control tissue specimens; significant differences were observed in the expressions of PI3K and P-Akt among the three groups of tissue specimens (F =44.87,20.64,respectively,both P ＜ 0.01 ).The results of Western blot were consistent with those of immunohistochemistry,and there was a significant difference in the gray scale value for PI3K and P-Akt between cervical squamous cell carcinoma,condyloma acuminatum and control tissue specimens (3.48 ± 0.48 vs.1.99 ± 0.11 vs.1.00 ± 0.03,F=354.83,P＜ 0.01; 3.33 ± 0.26 vs.1.96 ± 0.11 vs.1.00 ± 0.03,F=302.33,P＜ 0.01 ).Conclusions The PI3K/Akt signaling pathway is abnormally activated in condyloma acuminatum and cervical squamous cell carcinoma,and human papilloma virus may cause the abnormal proliferation of infected epithelium likely by affecting the upregnlated expression of PI3K/P-Akt.

Objective To investigate the clinical value of vitamin D receptor ( VDR) gene polymorphism in lung cancer screening .Methods From January 2017 to September 2017 ,2000 lung cancer screening patients in the Fifth People's Hospital of Foshan were selected in the research .The VDR gene polymorphism was determined by phenol-chloroform method .DNA was extracted from the peripheral blood of patients ,different VDR genotypes [ Bsm1 bb and Bb),Apal(aa,Aa and AA)] were analyzed by univariate analysis to determine the correlation between lung cancer and VDR gene polymorphism and lung cancer incidence by multivariate non -conditional logistic regression analysis, thus to explore the relationship between different VDR genotypes and risk factors of lung cancer . Results The level of serum vitamin D in the non -lung cancer patients was (46.5 ±2.3) ng/L,which was signifi-cantly higher than (26.5 ±1.1)ng/L in the lung cancer patients (t=49.614,P=0.000).The Bsm1bp genotype, Apal aa genotype and Apal Aa genotype were the risk factors of lung cancer .The Bsm1bp and Apal Aa genotypes were independent risk factors of lung cancer .Conclusion The Bsm1 locus and Apal locus Aa genotype in VDR receptor are high risk population of lung cancer .Therefore,we should pay more attention to the clinical screening and avoid misdiagnosis and missed diagnosis .

Objective To explore the effect of levamlodipine on contraction of isolated rat basilar arteries induced by 5-hydroxytryptamine (5-HT) in hypoxic condition and its mechanism.Methods Basilar arteries in SD rats were applied in this experiment,and divided into vascular endothelial group and endothelium intact group,and the arteries in the vascular endothelial group were perfused low ionic strength solution to remove the vascular endothelium.Mixed gases were passed continuously into the bath liquid during the experiment;according to the different objectives,95％ O2+5％ CO2 mixed gas was passed at normoxia condition,while 95％ N2+5％ CO2 gas mixture at hypoxia.Blood vessels from the two groups were carried out vascular endothelial integrity and stability test.After passing the test,5× 10-7 mol/L 5-HT was used to pre-contract blood vessel;(1) vessels were respectively incubated with different concentrations (10-8-10-3 mol/L) of levamlodipine for 15 min,and the diameter changes of vessels were determined at each time point using vascular tension system;(2) 10-4 mol/L L-NAME solution (nitric oxide synthase inhibitor) for 30 min at hypoxia and 10-4 mol/L of amlodipine for 15 min at normoxia condition were incubated into the vessels,respectively,and the diameter changes of rat basilar artery precontracted by 5-HT were also recorded.Results (1)The inhibitory percentage of levamlodipine at different concentration (10-8-10-3 mol/L) in the endothelium intact group was 0.0436％± 0.0116％,0.0530％±0.0134％,0.0696％±0.0103％,0.1265％±0.0284％,0.2362％±0.0275％,0.4084％± 0.0260％,0.5185％±0.0238％ and 0.5358％±0.0160％,respectively,and that in the vascular endothelial group was 0.0607％±0.0084％,0.0852％±0.0138％,0.1676％±0.0247％,0.3285％±0.0250％,0.4161％± 0.284％ and 0.4219％±0.260％;the inhibiting effect in the endothelium intact group was more obvious than that in the vascular endothelial group at hypoxic condition with significant difference (P＜0.05).(2) At the normal condition,the inhibitory percentage of levamlodipine was 0.9626％±0.0224％ and 0.7358％ ± ±0.0149％ before and after L-NAME treatment;at the hypoxic condition,the inhibitory percentage of levamlodipine was 0.9485％±0.0129％ 、0.8979％±0.0150％ before and after L-NAME treatment;the difference between the two conditions were significantly different (P＜0.05) Conclusion Levamlodipine has concentration-dependent inhibitory effects on vasoconstriction of rat basilar artery induced by 5-HT in hypoxic condition,which may be related to receptor-operated calcium channels and the release ofendothelium derived relaxing factor NO.

Objective:To explore the clinical characteristics of superior mesenteric artery ischemic diseases.Methods:The clinical and followup data of 141 hospitalized patients with ischemic disease of superior mesenteric artery in the Affiliated Hospital of Southwest Medical University from 1999 to 2021 were reviewed.Results:There were 99 males (70.2%) and 42 females (29.8%). The average age of the patients was (63.75±13.16) years; 127 patients (90.1%) complained abdominal pain.The number of all-cause deaths in the perioperative period was 27 (19.1%). ROC curve analysis showed that the optimal cutoff value of the age with predictive value was 64.5 years. The results of multivariate COX regression analysis showed that age ≥65 years old ( HR=3.855, 95% CI: 1.739-8.545), neutrophil count ( HR=1.072, 95% CI: 1.018-1.130), heart failure ( HR=2.863, 95% CI: 1.332-6.154), creatinine ( HR=1.009, 95% CI: 1.003-1.015), D-Dimer ( HR=1.112, 95% CI: 1.025-1.205) was an independent risk factor for all-cause death from superior mesenteric artery ischemic disease. Long-term survival rate of SMAD group was significantly higher than that of other SMAID; By comparing the clinical characteristics of different SMAID, neutrophil count, neutrophil ratio, D-dimer of SMAE group and SMAT group were significantly higher than that of SMAD group and ASSMA group. Conclusions:SMAID usually occurs in elderly men over 65 years old, with abdominal pain as the main symptom and often accompanied by hypertension. Risk factors included age, increased WBC count and D-dimer; The neutrophil count, neutrophil ratio, D-dimer in SMAE and SMAT group were significantly higher than that of SMAD and ASSMA group.