Objective To investigate the effect of nitric oxide (NO) on the scratching behavior evoked by substance P (SP) in mice. Methods Forty BALB/c mice were randomly divided into 5 groups to receive intradermal injection of different doses of SP (0, 20, 40, 80, 160 nmol/site) into the rostral part of the back to establish the acute itch model. Another 40 mice were randomly allocated to model, spantide, L-arginine,L-NAME and aminognanidine groups injected intracutaneously with normal saline (NS), spantide, L-arginine,L-NAME and aminoguanidine, respectively, 10 minutes before SP (80 nmol/site) injection. Subsequently,the scratching behavior was observed, iNOS expression and NO level in the injected skin were detected by immunohistochemical staining and nitrate reductase assay, respectively. Results The scratching bouts per hour induced by intradermal NS and SP of 20, 40, 80 and 160 nmol/site were 4.38±4.07, 5.38±3.78,12.75±6.52, 23.50±7.84 and 42.38±15.84, respectively, and only SP at higher doses (40 - 160 nmol/site)elicited a dose-dependent scratching response in mice (P < 0.01 or 0.05) compared with NS. The scratches over 1 hour in SP, L-arginine, spantide, L-NAME and aminognanidine group were 67.13±32.79, 70.75±34.80, 10.75±8.14, 29.00±21.19 and 35.38±22.83, respectively; of them, pretreatment with spantide,L-NAME and aminognanidine significantly inhibited SP-induced scratching (P < 0.01 or 0.05), but L-arginine showed no inhibitory effect (P > 0.05). Compared with SP, the pretreatment with spantide, L-NAME and aminoguanidine significantly downregulated the iNOS expression and NO content (P < 0.01 or 0.05) in the injected skin other than L-arginine (P > 0.05 ). Conclusion Intradermal SP could increase NO synthesis by neurokinin 1 receptor activation, resulting in the scratching behavior in BALB/c mice.

Objective To establish a rabbit model of restenosis and analyze the expressions of VEGFmRNA and TGF-?_1mRNA during the intimal proliferation.We also explored the relationship between VEGFmRNA,TGF-?_1mRNA and restenosis.Methods 40 healthy male New Zealand white rabbits were evenly divided into three injury groups and one control group.Right carotid arteries were injured with PCI balloon in the injury groups.10 rabbits of each injury group were sacrificed on weeks 1,2 and 4 after the injury.VEGFmRNA and TGF-?_1mRNA were examined by in situ hybridization.All the samples were analyzed using a computerized imaging analysis system.Results In the injury groups,neointimal areas were significantly larger than those in control group(P

Obj ective To summarize yhe nursing experience of payienys wiyh lumbar disc herniayion yreayed wiyh percuyaneous posyerolayeral foraminoplasyy.Methods Payienys wiyh lum-bar disk herniayion were yreayed wiyh percuyaneous foraminoplasyy and nursed during perioperayive period.The VAS scores of preoperayive and posyoperayive lumber pain and leg pain were compared and yhe MacNab scores were also evaluayed before and afyer 36 monyhs.Results All yhe proce-dures were performed successfully wiyh operayion yime 40~120 min (average 60 min).Follow-up was conyinued for more yhan 36 monyhs.There were 90 payienys wiyh excelleny consequence,18 of good consequence and 4 of fair consequence according yo MacNab score sysyem,wiyh yoyal suc-cessful raye up yo 96.4%.Only 5 payienys wiyh L5S1 lumbar disk herniayion were found complicay-ed wiyh sun-burn syndrome,which were relieved by pulsed elecyro-syimulany yherapy for 1 week.Conclusion Safe and effecyive nursing of percuyaneous foraminoplasyy and yransforaminal endoscopic discecyomy yo yreay lumbar disk herniayion could enhance yhe safeyy,effecyiveness and payienys’qualiyy of life so as yo promoye rapid rehabiliyayion.

Obj ective To summarize yhe nursing experience of payienys wiyh lumbar disc herniayion yreayed wiyh percuyaneous posyerolayeral foraminoplasyy.Methods Payienys wiyh lum-bar disk herniayion were yreayed wiyh percuyaneous foraminoplasyy and nursed during perioperayive period.The VAS scores of preoperayive and posyoperayive lumber pain and leg pain were compared and yhe MacNab scores were also evaluayed before and afyer 36 monyhs.Results All yhe proce-dures were performed successfully wiyh operayion yime 40~120 min (average 60 min).Follow-up was conyinued for more yhan 36 monyhs.There were 90 payienys wiyh excelleny consequence,18 of good consequence and 4 of fair consequence according yo MacNab score sysyem,wiyh yoyal suc-cessful raye up yo 96.4%.Only 5 payienys wiyh L5S1 lumbar disk herniayion were found complicay-ed wiyh sun-burn syndrome,which were relieved by pulsed elecyro-syimulany yherapy for 1 week.Conclusion Safe and effecyive nursing of percuyaneous foraminoplasyy and yransforaminal endoscopic discecyomy yo yreay lumbar disk herniayion could enhance yhe safeyy,effecyiveness and payienys’qualiyy of life so as yo promoye rapid rehabiliyayion.

Objective:To construct the performance evaluation model of hospital information engineer to solve the scientific and objective problems of performance evaluation of hospital information engineer.Methods:AAccording to the requirements of the hospital for the overall performance objectives for the information department,in accordance with the principle of combining qualitative and quantitative analysis,the performance objectives were quantitatively decomposed into five first-level evaluation indicators of core literacy,skill level,work performance,scientific research and teaching,and major tasks,and 23 second-level indicators were refined.The subjective weighting method of the analytic hierarchy process(AHP)and the objective weighting method of entropy method were used to comprehensively calculate and establish the index weight coefficient,and the performance evaluation model of hospital information engineers was constructed.Five information engineers(marked as A,B,C,D and E)from the 900th Hospital of the Joint Logistics Support Force in 2022 were randomly selected by a simple random sampling method for performance evaluation.By collecting relevant data and using model evaluation calculation,the feasibility of the evaluation model was determined by comparing the annual performance level of engineers.Results:After comprehensive calculation,the comprehensive evaluation scores of five information engineers(A,B,C,D and E)were 87.46,80.39,60.38,76.33 and 70.33,respectively.The top engineers,A and B,with the total score were excellent in the annual performance evaluation of the hospital in 2022,and the information engineer C in the lower ranking was evaluated as qualified in the annual performance evaluation of the hospital in 2022,which were consistent with the results of comprehensive calculation and evaluation.Conclusion:The performance evaluation model of hospital information engineers based on AHP and entropy method can objectively and truly evaluate the performance of hospital information engineers,fully mobilize the enthusiasm and creativity of information engineers,promote the scientific and objective evaluation of the performance of hospital information engineers.It can also support the establishment of the evaluation mechanism and the implementation of incentive measures of hospital information department and ensure the construction requirements of information talent team for high-quality development of hospitals.

Objective To investigate the expression of long-chain non-coding RNA gastric cancer high expression transcription factor 1 (GHET1) in hepatocellular carcinoma (HCC) and the correlation with prognosis,cell proliferation,migration and invasion.Methods 20 HCC patients who underwent surgery from Fuzhou General Hospital of Chinese People's Liberation Army from March to May 2016 were included.The HCC tissue and adjacent normal tissue of 182 patients from June 2012 to December 2013 were retrospectively collected.According to the median value of GHET1 expression,it was divided into GHET1 high expression group and low expression group,91 cases each.Huh7 and HepG2 cells were divided into:blank control group (Con) with serum-free medium,siRNA-GHET1 group transfected with siRNA-GHET1,and negative control group (siRNA-NC) transfected with negative control sequence.The expression of GHET1 was detected by real-time fluorescence quantitative polymerase chain reaction,and the effect of GHET1 on HCC cells was analyzed by CCK-8,Transwell assay and Western blot.Results Compared with adjacent normal tissue,the relative expression of GHET1 mRNA in HCC tissues was significantly increased.Compared with LO2 cells,the mRNA expression of GHET1 in Huh7 and HepG2 cells was higher (P＜0.05).The GHET1 high expression group had tumor＞5 cm,vascular invasion,AFP＞400 μg/L,Edmonson grade Ⅰ,and the tumor-free ratio was lower in the expression group (P＜0.05).Survival analysis showed that HCC patients with high GHET1 expression had a poorer prognosis than patients with low expression.Multivariate Cox regression analysis showed that high expressed GHET1,vascular invasion (HR=2.067,95％ CI:1.350 to 3.162),and without tumor capsule are independent predictors of recurrence in HCC patients.After transfection with Huh7 and HepG2 cells,the proliferation of siRNA-GHET1 group was significantly decreased comparing with Con and siRNA-NC groups.Compared with siRNA-NC group,the migration and invasion ability of siRNA-GHET1 group decreased,and E-cadherin expression increased.The expression of fibronectin and vimentin decreased,and the difference was statistically significant (P＜0.05).Conclusions The expression of GHET1 in HCC tissue is higher comparing with normal tissue,which increases the proliferation,migration and invasion of hepatoma cells.It is an independent predictor of prognosis in HCC patients and a potential target for clinical treatment.