1.Identification on biological characteristics of AIV H5N1 monoclonal antibody
Jingli LI ; Haixiang ZHANG ; Yangmeng FENG ; Guanghua WANG ; Yongnian LIU ; Jun HU
Chinese Journal of Immunology 2017;33(3):398-400,406
Objective:To investigate the biological characteristics of monoclonal antibodies against avian influenza virus (AIV).Methods:Monoclonal antibodies (mAbs) against AIV H5N1 were prepared and it′s characteristics were identified including subtype,titer,hemagglutination inhibition activity and cross-reactivity with other influenza viruses.Besides,Western blot and immuno-histochemical staining methods were conducted to test the combination of antibodies and antigen ( H5N1 ) and human normal tissues.Results:Immunohistochemical analysis showed that 2 mAbs (H5-32 and H5-35) cross-reacted with human tissues kidney and pancreas respectively.Conclusion:These data indicated that there have some association between the AIV H 5N1 with human tissues, which may provide reference for the study on avian influenza virus infection and pathogenicity .
2.Notoginsenoside Ft1 acts as a TGR5 agonist but FXR antagonist to alleviate high fat diet-induced obesity and insulin resistance in mice.
Lili DING ; Qiaoling YANG ; Eryun ZHANG ; Yangmeng WANG ; Siming SUN ; Yingbo YANG ; Tong TIAN ; Zhengcai JU ; Linshan JIANG ; Xunjiang WANG ; Zhengtao WANG ; Wendong HUANG ; Li YANG
Acta Pharmaceutica Sinica B 2021;11(6):1541-1554
Obesity and its associated complications are highly related to a current public health crisis around the world. A growing body of evidence has indicated that G-protein coupled bile acid (BA) receptor TGR5 (also known as Gpbar-1) is a potential drug target to treat obesity and associated metabolic disorders. We have identified notoginsenoside Ft1 (Ft1) from
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