Objective To evaluate the repair of benign constriction of the porta hepatic duct by pedicled autograft. Methods The clinical data of repair by pedicled valve of gallbladder in 38 cases, by pedicled gastric wall in 2 cases and by round ligament of the liver in 2 cases were retrospectively analyzed. Results There were no operative mortality and no complications such as bile leaks and hemorrhage. Cholangiography through T-tube showed patency in all postoperative patients. A follow-up from 6 months to 14 years in 40 out of 42 cases found cholangitis in 2 patients with good response to conservative therapy. Conclusions Treatment of benign stricture of porta hepatic duct by pedicled autograft is physiologic, convenient and effective.

Rhodotorula toruloides is a non-conventional red yeast that can synthesize various carotenoids and lipids. It can utilize a variety of cost-effective raw materials, tolerate and assimilate toxic inhibitors in lignocellulosic hydrolysate. At present, it is widely investigated for the production of microbial lipids, terpenes, high-value enzymes, sugar alcohols and polyketides. Given its broad industrial application prospects, researchers have carried out multi-dimensional theoretical and technological exploration, including research on genomics, transcriptomics, proteomics and genetic operation platform. Here we review the recent progress in metabolic engineering and natural product synthesis of R. toruloides, and prospect the challenges and possible solutions in the construction of R. toruloides cell factory.
Gene Editing ; Metabolic Engineering ; Rhodotorula/metabolism* ; Lipids

Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO) cells were transfected with pEGFP-N1-Fyn. The expression of Fyn mRNA and proteins was monitored by reverse transcription-PCR and Western blotting. Additionally, transfected cells were stained with 4',6-diamidino-2-phenylindole and a series of time-lapse images was taken. Sequences of the recombinant plasmids pMD18-T-Fyn and pEGFP-N1-Fyn were confirmed by sequence identification using National Center for Biotechnology Information in USA, and Fyn expression was detected by RT-PCR and Western blotting. The morphology of CHO cells transfected with the recombinant vector was significantly altered. Fyn expression induced filopodia and lamellipodia formation. Based on these results, we concluded that overexpression of mouse Fyn induces the formation of filopodia and lamellipodia in CHO cells, and promotes cell movement.
Animals ; Blotting, Western ; CHO Cells ; Cricetinae ; Cricetulus ; Genetic Vectors ; Green Fluorescent Proteins/genetics ; Mice ; Proto-Oncogene Proteins c-fyn/genetics/*metabolism ; Pseudopodia/*metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Time-Lapse Imaging ; Transfection

Objective To investigate the changes of electrocardiogram in elderly patients with acute cerebral ischemic infarction (CIS),and to analyze the relationship between the electrocardiogram and the prognosis of the patients.Methods 132 elderly patients with acute CIS in the hospital from January 2013 to December 2016 were enrolled.12 lead electrocardiogram was performed within 48 hours after onset and 7 days after onset,and the relationship between electrocardiogram abnormality and infarct type,severity,and prognosis were analyzed.The independent predictors of poor prognosis based on improved Rankin's score at discharge were evaluated.Results 83 cases (62.88％) had abnormal electrocardiogram.The main type of abnormal electrocardiogram was S-T segment abnormalities,followed by arrhythmia.The severity of illness in patients with abnormal electrocardiogram were significantly more serious than in those who did not detect abnormal electrocardiogram (P ＜ 0.05).The severity of illness in patients detected abnormal electrocardiogram over 2 times were significantly more serious than in those who detected abnormal electrocardiogram only within 48 hours after onset or 7 days after onset (P ＜ 0.05).Shorter time from onset to admission,complete anterior circulation infarction according to Oxfordshire Community Stroke Project (OCSP)classification,abnormal electrocardiogram (＞ 2 times) were independent risk factors for poor prognosis at discharge (P ＜ 0.05).Conclusions Electrocardiogram abnormity is common in elderly patients with CIS,and abnormal electrocardiogram detected over 2 times may indicate poor prognosis,which will benefit for the treatment schemes of patients.

OBJECTIVETo investigate the constituents of Ervatamia hainanensis systematically.

METHODVarious chromatographic techniques were applied to isolate and purify the constituents of this plant. The structures were elucidated by spectroscopic analysis.

RESULTEight compounds were obtained, which were identified as alpha-amyrin acetate (1), 11-oxo-alpha-amyrin acetate (2), beta-sitosterol (3), cycloart-23-ene-3beta, 25-diol(4), cycloart-25-ene-3beta, 24-diol (5), 5alpha, 8alpha-epidioxyergosta-6, 22-dien-3beta-ol (6), ibogamin-3-one (7), beta-daucosterol (8).

CONCLUSIONCompounds 1, 2, 4- 7 were isolated from this plant for the first time.

Apocynaceae ; chemistry ; Ergosterol ; analogs & derivatives ; chemistry ; isolation & purification ; Oleanolic Acid ; analogs & derivatives ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Triterpenes ; chemistry ; isolation & purification

Hepatitis B virus X protein (HBx) has various functions and plays a crucial role in the development of hepatocellular carcinoma (HCC). However, due to different transfection efficiency levels and experimental approaches, it is difficult to correlate the exact functions of HBx to HBV-associated HCC. In this study, we constructed two prokaryotic expression vectors, pGEX-HBx-EGFP-TLM and pGEX-EGFP-TLM, which expressed HBx-EGFP-TLM and EGFP-TLM fusion proteins respectively. Both vectors contained a coding sequence of TLM transduction motif derived from the PreS2-domain of Hepatitis B Virus surface antigens. In addition, EGFP was expressed as a reporter reflecting the transduction efficiency of TLM. The fusion protein HBx-EGFP-TLM or EGFP-TLM purified from Escherichia coli BL21(DE3) by AKTA Purifier system was incubated with AML12 and SMMC-7721 cells. Both Western blotting and laser confocal results indicated that the translocation motif TLM could lead HBx-EGFP and EGFP into the cytoplasm. Dual-Luciferase Reporter Assay revealed that the activity of mEZH2 promoter could be up-regulated by the recombinant HBx. In conclusion, we expressed a cell-permeable HBx, which could provide a new method to study the functions of HBx.
Amino Acid Motifs ; genetics ; Cell-Penetrating Peptides ; biosynthesis ; genetics ; Escherichia coli ; genetics ; metabolism ; Genetic Vectors ; genetics ; Green Fluorescent Proteins ; biosynthesis ; genetics ; Hepatitis B Surface Antigens ; genetics ; Protein Precursors ; genetics ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; isolation & purification ; Trans-Activators ; biosynthesis ; genetics

Objective To investigate the knowledge,attitude and practice (KAP) of blood glucose monitoring and management among non-diabetes specialized nurses in a tertiary hospital of Xiamen,and provide basis for the late intervention.Methods With convenient sampling method,409 non-diabetes specialized nurses from a tertiary hospital of Xiamen were selected as the research object,and they were investigated by self-designed blood sugar monitoring and nursing KAP questionnaire.Results The score of knowledge related to blood sugar monitoring management was (6.52±1.39);the score of attitude related to blood sugar monitoring management was (45.94±4.18);the score of practice related to blood sugar monitoring management was (56.16±6.37);the attitude related to blood glucose monitoring management of non-diabetes specialized nurses was positively correlated with their practice (r=0.119,P=0.016);and nurses' practice was also positively correlated with their knowledge (r=0.190,P=0.027). Among 409 nurses,the percentage of nurses' self-rating mastery degree,general mastery degree,less familiar and unfamiliar in blood sugar management knowledge were 54.8%,37.9%,4.4 and 0.5% %,and only 2.4% of nurses thought they completely master the knowledge. 85.5% of nurses had received training,training organized by nursing department and training in department which includes diabetes link nurses were dominant,and they were 64.1% and 31.5%. The training method was single (72.6%). Theoretical teaching in hospital was still the most popular way,and it accounted for 78.5%. By multiple linear regression analysis,service year was the influencing factors of blood sugar monitoring management. ConclusionsThe knowledge that non-diabetes specialist nurses master is not optimistic,and the level of KAP in blood sugar monitoring still needs to be improved,while full standardization and diversity training are effective methods.

To optimize program of bovine somatic nuclear transfer, we used two different enucleation procedures (by Spindle-view system & Hoechst 33342 staining), two different procedures to introduce donor nuclei (by ooplasm microinjection & electrofusion), and three different group electrofusion parameters (group 1: 1.9 kV/cm, 10 micros, two; group 2: 1.5 kV/cm, 25 micros, two; group 3: 0.6 kV/cm, 100 micros, one) to reconstruct bovine cloned embryos. The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 micros, two) to reconstruct bovine cloned embryos. Then the excellent blastocysts were transferred to fosters for producing cloned cattle 80 high-quality cloned blastocysts were transferred into 33 fosters, two cloned calves were produced. According to the results, the optimized program could be used to produce cloned cattle.
Animals ; Cattle ; Cell Nucleus ; physiology ; Cloning, Organism ; veterinary ; Embryo Transfer ; methods ; Embryo, Mammalian ; cytology ; physiology ; Female ; Microinjections ; Nuclear Transfer Techniques ; veterinary ; Oocytes ; cytology ; physiology

Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migration of both early- and late-born neurons during mouse corticogenesis. Moreover, Cotl1 overexpression delayed, rather than blocked, neuronal migration in late-born neurons. Cotl1 expression disturbed the morphology of migrating neurons, lengthening the leading processes. This study is the first to investigate the function of Cotl1, and the results indicate that Cotl1 is involved in the regulation of neuronal migration and morphogenesis.
Actins ; Animals ; Dictyostelium ; Humans ; Mice ; Morphogenesis ; Neurons