Background Pars plana vitrectomy (PPV) is the main therapy for advanced stage of proliferative diabetic retinopathy (PDR).Intravitreal injection of anti-vascular endothelial growth factor(VEGF) drug before PPV for PDR has been proved to decrease the occurrence of postoperative complications.However,the effect of conbercept on PPV was rarely reported.Objective This study was to observe the efficacy of intravitreal injection of conbercept (IVC) on PPV for PDR.Methods A nonrandomized control clinical trial was performed.A total of 47 PDR patients (51 eyes) undergoing PPV were enrolled in Yuebei People's Hospital from June 2015 to May 2016.All subjects were divided into IVC group (24 patients,26 eyes) and control group (23 patients,25 eyes).The incidence of iatrogenic retinal hole,rate of using intraocular tamponade,incidence of postoperative complications,central retinal thickness (CRT) and the best corrected visual acuity (BCVA) were comparatively analyzed.Results The incidence of iatrogenic retinal hole in IVC group was 7.69％,lower than 32.00％ in the control group,the difference was statistically significant (P=0.038).The percentage of using intraocular tamponade in IVC group was 19.23％,lower than 52.00％ in the control group,the difference was statistically significant (x2 =5.993,P =0.014).Four months after surgery,the incidence of postoperative vitreous re-hemorrhage in IVC group was significantly lower than that in the control group,the difference was statistically significant (P =0.024).Three months after surgery,the CRT in IVC group was (278.04 ± 43.46) μm,which is thinner than (340.76 ± 84.91) μm in the control group,the difference was statistically significant (t =-3.340,P =0.002).Three months after surgery,the BCVA in IVC group was better than that in control group,with significant difference between the two groups (Z =-2.114,P=0.034).No adverse effect was observed after IVC.Conclusions IVC before PPV for PDR can reduce the incidence of iatrogenic retinal hole and the rate of using intraocular tamponade,decrease the incidence of postoperative complications and macular retinal thickness,and improve the visual acuity.

ObjectiveTo analyze the supplementary test results of HIV screening positive samples in Jianyang City, Chengdu from 2019 to 2022, to evaluate different HIV testing methods, and to provide a basis for the development of HIV testing strategies in the local area. MethodsWestern blotting (WB) supplementary test was conducted on 1 172 screening positive samples from the HIV confirmatory laboratory in 2019‒2022 according to the national technical specifications. The samples were tested by the rapid test, enzyme-linked immunoassay (ELISA), and chemiluminescence immunoassay (CLIA). The test results of the three HIV screening methods were collected and a database was established for statistical analysis. ResultsA total of 1 172 samples were tested through supplementary test, of which 1 022 samples were tested positive (87.20%), 75 were uncertain (6.40%), and 75 were negative (6.40%). The positive results of the three different HIV screening methods were consistent with the supplementary test. The rapid test had the highest positively supplementary rate of 88.54%, followed by ELISA of 86.98%, and CLIA of 85.92%. The difference was statistically significant (χ²=9.505, P<0.05). The detection rate of WB band patterns in positive samples were the highest at 100.00% for gp160 and gp120, and lowest at 50.68% and 63.41% for p55 and p17, respectively. The WB band patterns of uncertain samples were mainly gp120 (81.33%) and p24 (46.67%). Among the 75 uncertain samples, 39 were followed up and 29 of which turned positive, with a high positive conversion rate of 74.36%. ConclusionIt is necessary to directly add HIV nucleic acid testing to samples with positive WB supplementary test results and samples with uncertain WB supplementary test results in combination with CLIA, so as to avoid the spread of HIV infection caused by missed detections.