OBJECTIVETo establish a HPLC method for the analysis of Danshensu (DSS), protocatechuic acid (PA) and protocatechuic aldehyde (PAL) of Xiangdan injection in rat's plasma, and to study pharmacokinetic characteristics of Xiangdan injection components in rats with m-hydroxybenzoic acid as internal standard.

METHODprotein was precipitated by 10% trichloroacetic acid and extracted by ethyl acetate. The plasma concentration was detected by HPLC, The pharmacokinetics parameters of DSS, PA and PAL were calculated by DAS2.0 software after iv injection.

RESULTDSS, PA and PAL have a good linear relationship in 0.68-44.0 mg x L(-1), 0.43-14.0 mg x L(-1) and 0.38-12.0 mg x L(-1), respectively. The average recoveries were more than 92% and the RSD of precision and stability of the test were between 0.4%-4.8%. DSS, PA and PAL showed a two-compartment open model, the half-life of absorption (t(1/2alpha)) were (6.26 +/- 4.6), (5.93 +/- 4.9), (18.44 +/- 2.4) min, the half-life of elimination (t(1/2beta)) were (64.11 +/- 8.8), (63.28 +/- 0.13), (69.315 +/- 0) min, the area under curve(AUC(0-infinity)) were (852.98 +/- 175.6), (83.84 +/- 58.8), (147.79 +/- 12.3) mg x min(-1) x L(-1).

CONCLUSIONA method with high recovery rate and good stability was established to determine the blood concentration of DSS, PA, PAL in Xiangdan injection and applied in its pharmacokinetics successfully.

Animals ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; Female ; Hydroxybenzoates ; blood ; pharmacokinetics ; Injections ; Limit of Detection ; Linear Models ; Male ; Rats ; Rats, Sprague-Dawley

Objective To investigate clinical characteristics and risk factors in early and advanced menopausal women with coronary heart disease (CHD).Methods A total of 259 menopausal women with CHD confirmed by coronary angiography in our hospital were selected as research objects,among whom 102 cases were in menopause≤ 10 years group,and 157 cases in menopause ＞ 10 years group.The clinical characteristics and risk factors of two groups were compared.Results The menopause ≤ 10 years group had higher incidence rate of hypertension and estrogen levels(P ＜ 0.05),but there was no significant difference in HLD-C,and TC levels,and carotid intima-media thickness (IMT) reached the degree of atherosclerosis.Menopause ＞ 10 years group showed a significantly higher incidence of diabetes,but LDL-C level and estrogen levels was significantly lower (P ＜ 0.05).The coronary artery disease in the menopause≤ 10 years group mainly occurred in single lesion,while the menopause ＞ 10 years group in double or multi-vessel lesion (P ＜0.05).Multivariate analysis showed that hypertension,diabetes,triglycerides,low-density lipoprotein cholesterol were risk factors for coronary heart disease,while estradiol was a protective factor.Conclusion Hypertension,diabetes,triglycerides,and low-density lipoprotein cholesterol and decreased estrogen are risk factors for women with coronary heart disease.Conditions of vascular endothelium and atherosclerosis of early postmenopausal women are better than the advanced postmenopausal women,and estrogen alternative is favorable for the patients.

Objective To investigate clinical characteristics and risk factors in early and advanced menopausal women with coronary heart disease (CHD).Methods A total of 259 menopausal women with CHD confirmed by coronary angiography in our hospital were selected as research objects,among whom 102 cases were in menopause≤ 10 years group,and 157 cases in menopause ＞ 10 years group.The clinical characteristics and risk factors of two groups were compared.Results The menopause ≤ 10 years group had higher incidence rate of hypertension and estrogen levels(P ＜ 0.05),but there was no significant difference in HLD-C,and TC levels,and carotid intima-media thickness (IMT) reached the degree of atherosclerosis.Menopause ＞ 10 years group showed a significantly higher incidence of diabetes,but LDL-C level and estrogen levels was significantly lower (P ＜ 0.05).The coronary artery disease in the menopause≤ 10 years group mainly occurred in single lesion,while the menopause ＞ 10 years group in double or multi-vessel lesion (P ＜0.05).Multivariate analysis showed that hypertension,diabetes,triglycerides,low-density lipoprotein cholesterol were risk factors for coronary heart disease,while estradiol was a protective factor.Conclusion Hypertension,diabetes,triglycerides,and low-density lipoprotein cholesterol and decreased estrogen are risk factors for women with coronary heart disease.Conditions of vascular endothelium and atherosclerosis of early postmenopausal women are better than the advanced postmenopausal women,and estrogen alternative is favorable for the patients.